Light microscopy diagnosis of Babesia bovis and Babesia bigemina kinetes in the haemolymph of artificially infected Boophilus microplus engorged female ticks
The length, width and position of the nucleus of Babesia bovis and Babesia bigemina kinetes from the haemolymph of Boophilus microplus engorged female ticks were recorded. Additionally, the shape of Babesia bovis kinetes were registered as curved, semi-curved or straight. To this aim Boophilus micro...
Gespeichert in:
| Veröffentlicht in: | Veterinary parasitology 1996, Vol.61 (1), p.15-20 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 20 |
|---|---|
| container_issue | 1 |
| container_start_page | 15 |
| container_title | Veterinary parasitology |
| container_volume | 61 |
| creator | Guglielmone, A.A. Gaido, A.B. Mangold, A.J. |
| description | The length, width and position of the nucleus of
Babesia bovis and
Babesia bigemina kinetes from the haemolymph of
Boophilus microplus engorged female ticks were recorded. Additionally, the shape of
Babesia bovis kinetes were registered as curved, semi-curved or straight. To this aim
Boophilus microplus tick larvae from a colony free of
Babesia were fed on splenectomised calves artificially infected with either
Babesia bovis or
Babesia bigemina pathogenic strains. Six engorged female ticks showing an infection of at least ten mature kinetes of
Babesia bovis in a sample of haemolymph 5 days after detachment were also monitored 7, 9 and 10 days after collection. The same procedure was followed with six engorged female ticks infected with
Babesia bigemina.
One hundred and twenty kinetes of each species of
Babesia were evaluated. The mean length ± standard deviation and ranges for
Babesia bovis kinetes were 14.30 ± 0.922
μm and 11.9−16.3
μm, while the corresponding measures for the kinetes of
Babesia bigemina were 11.27 ± 0.900
μm and 9.0−13.1
μm (
P < 0.001,
t-test). The width was 3.33 ± 0.315
μm, 2.6−4.0
μm for
Babesia bovis and 2.24 ± 0.287
μm, 1.5−2.8
μm for
Babesia bigemina kinetes (
P < 0.001). The most common position of the nucleus was central for both species of
Babesia. A total of 58% of
Babesia bovis kinetes showed the typical curved tail. No effect of time post-collection and individual host ticks in the kinete of
Babesia bigemina was found while an unexpected influence of individual host tick in the width of
Babesia bovis kinetes was detected (
P < 0.01, analysis of variance).
The overlap in the sizes of kinetes from both species of
Babesia makes it difficult to apply the results to ticks of unknown babesial infection status. This finding is further complicated by the intra-specific size variations of
Babesia kinetes from different geographical origins. |
| doi_str_mv | 10.1016/0304-4017(95)00813-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_78262177</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0304401795008136</els_id><sourcerecordid>15782148</sourcerecordid><originalsourceid>FETCH-LOGICAL-c473t-ad15cfd0120a3c748e5a00b59747295d797e0e9eb16e5ec18ac06b044846c6cd3</originalsourceid><addsrcrecordid>eNqFUcuK1UAQbUQZr6M_IApZiS6i1Uk_ks2ADr7gohtdN51OJbecJB27cwfux_ivdsxl3OmqijqPgnMYe8bhNQeu3kAJIhfA9ctavgKoeJmre2zHK13mhZRwn-3uKA_Zoxh_AIAApS_YRaVlWuod-7Wn_rBkI7ngo_PzKWvJ9pOPFDPfZe9sg5Fs1vjbdLBT-_dCPY402eyGJlwwZjRlywGzg8XRD6dxPqx6GxbqyJEdhlNidOgWTB7ezwcajnH7O68bTr0PfQI7HO2A2ULuJj5mDzo7RHxynpfs-4f3364_5fuvHz9fv93nTuhyyW3Lpeta4AXY0mlRobQAjay10EUtW11rBKyx4QolOl5ZB6oBISqhnHJteclebL5z8D-PGBczUnQ4DHZCf4xGV4UquNb_JXKZqFxUiSg24pprDNiZOdBow8lwMGt9Zu3GrN2YWpo_9RmVZM_P_sdmxPZOdO4r4U83vLPe2D5QNF_2tUryQibwagMxRXVLGEx0hJPDlkIK3rSe_v39N6_XtIc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15782148</pqid></control><display><type>article</type><title>Light microscopy diagnosis of Babesia bovis and Babesia bigemina kinetes in the haemolymph of artificially infected Boophilus microplus engorged female ticks</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Guglielmone, A.A. ; Gaido, A.B. ; Mangold, A.J.</creator><creatorcontrib>Guglielmone, A.A. ; Gaido, A.B. ; Mangold, A.J.</creatorcontrib><description>The length, width and position of the nucleus of
Babesia bovis and
Babesia bigemina kinetes from the haemolymph of
Boophilus microplus engorged female ticks were recorded. Additionally, the shape of
Babesia bovis kinetes were registered as curved, semi-curved or straight. To this aim
Boophilus microplus tick larvae from a colony free of
Babesia were fed on splenectomised calves artificially infected with either
Babesia bovis or
Babesia bigemina pathogenic strains. Six engorged female ticks showing an infection of at least ten mature kinetes of
Babesia bovis in a sample of haemolymph 5 days after detachment were also monitored 7, 9 and 10 days after collection. The same procedure was followed with six engorged female ticks infected with
Babesia bigemina.
One hundred and twenty kinetes of each species of
Babesia were evaluated. The mean length ± standard deviation and ranges for
Babesia bovis kinetes were 14.30 ± 0.922
μm and 11.9−16.3
μm, while the corresponding measures for the kinetes of
Babesia bigemina were 11.27 ± 0.900
μm and 9.0−13.1
μm (
P < 0.001,
t-test). The width was 3.33 ± 0.315
μm, 2.6−4.0
μm for
Babesia bovis and 2.24 ± 0.287
μm, 1.5−2.8
μm for
Babesia bigemina kinetes (
P < 0.001). The most common position of the nucleus was central for both species of
Babesia. A total of 58% of
Babesia bovis kinetes showed the typical curved tail. No effect of time post-collection and individual host ticks in the kinete of
Babesia bigemina was found while an unexpected influence of individual host tick in the width of
Babesia bovis kinetes was detected (
P < 0.01, analysis of variance).
The overlap in the sizes of kinetes from both species of
Babesia makes it difficult to apply the results to ticks of unknown babesial infection status. This finding is further complicated by the intra-specific size variations of
Babesia kinetes from different geographical origins.</description><identifier>ISSN: 0304-4017</identifier><identifier>EISSN: 1873-2550</identifier><identifier>DOI: 10.1016/0304-4017(95)00813-6</identifier><identifier>PMID: 8750679</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Babesia - cytology ; Babesia - isolation & purification ; BABESIA BIGEMINA ; BABESIA BOVIS ; Babesia bovis - cytology ; Babesia bovis - growth & development ; Babesia bovis - isolation & purification ; BOOPHILUS MICROPLUS ; CALVES ; Cattle ; DIAGNOSIS ; Diagnosis-Protozoa ; DIAGNOSTIC ; DIAGNOSTICO ; Female ; HAEMOLYMPH ; HEMOLINFA ; Hemolymph - parasitology ; HEMOLYMPHE ; Ixodidae ; Microscopy - methods ; Species Specificity ; TERNERO ; Ticks - parasitology ; VEAU</subject><ispartof>Veterinary parasitology, 1996, Vol.61 (1), p.15-20</ispartof><rights>1996</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c473t-ad15cfd0120a3c748e5a00b59747295d797e0e9eb16e5ec18ac06b044846c6cd3</citedby><cites>FETCH-LOGICAL-c473t-ad15cfd0120a3c748e5a00b59747295d797e0e9eb16e5ec18ac06b044846c6cd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0304-4017(95)00813-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8750679$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guglielmone, A.A.</creatorcontrib><creatorcontrib>Gaido, A.B.</creatorcontrib><creatorcontrib>Mangold, A.J.</creatorcontrib><title>Light microscopy diagnosis of Babesia bovis and Babesia bigemina kinetes in the haemolymph of artificially infected Boophilus microplus engorged female ticks</title><title>Veterinary parasitology</title><addtitle>Vet Parasitol</addtitle><description>The length, width and position of the nucleus of
Babesia bovis and
Babesia bigemina kinetes from the haemolymph of
Boophilus microplus engorged female ticks were recorded. Additionally, the shape of
Babesia bovis kinetes were registered as curved, semi-curved or straight. To this aim
Boophilus microplus tick larvae from a colony free of
Babesia were fed on splenectomised calves artificially infected with either
Babesia bovis or
Babesia bigemina pathogenic strains. Six engorged female ticks showing an infection of at least ten mature kinetes of
Babesia bovis in a sample of haemolymph 5 days after detachment were also monitored 7, 9 and 10 days after collection. The same procedure was followed with six engorged female ticks infected with
Babesia bigemina.
One hundred and twenty kinetes of each species of
Babesia were evaluated. The mean length ± standard deviation and ranges for
Babesia bovis kinetes were 14.30 ± 0.922
μm and 11.9−16.3
μm, while the corresponding measures for the kinetes of
Babesia bigemina were 11.27 ± 0.900
μm and 9.0−13.1
μm (
P < 0.001,
t-test). The width was 3.33 ± 0.315
μm, 2.6−4.0
μm for
Babesia bovis and 2.24 ± 0.287
μm, 1.5−2.8
μm for
Babesia bigemina kinetes (
P < 0.001). The most common position of the nucleus was central for both species of
Babesia. A total of 58% of
Babesia bovis kinetes showed the typical curved tail. No effect of time post-collection and individual host ticks in the kinete of
Babesia bigemina was found while an unexpected influence of individual host tick in the width of
Babesia bovis kinetes was detected (
P < 0.01, analysis of variance).
The overlap in the sizes of kinetes from both species of
Babesia makes it difficult to apply the results to ticks of unknown babesial infection status. This finding is further complicated by the intra-specific size variations of
Babesia kinetes from different geographical origins.</description><subject>Animals</subject><subject>Babesia - cytology</subject><subject>Babesia - isolation & purification</subject><subject>BABESIA BIGEMINA</subject><subject>BABESIA BOVIS</subject><subject>Babesia bovis - cytology</subject><subject>Babesia bovis - growth & development</subject><subject>Babesia bovis - isolation & purification</subject><subject>BOOPHILUS MICROPLUS</subject><subject>CALVES</subject><subject>Cattle</subject><subject>DIAGNOSIS</subject><subject>Diagnosis-Protozoa</subject><subject>DIAGNOSTIC</subject><subject>DIAGNOSTICO</subject><subject>Female</subject><subject>HAEMOLYMPH</subject><subject>HEMOLINFA</subject><subject>Hemolymph - parasitology</subject><subject>HEMOLYMPHE</subject><subject>Ixodidae</subject><subject>Microscopy - methods</subject><subject>Species Specificity</subject><subject>TERNERO</subject><subject>Ticks - parasitology</subject><subject>VEAU</subject><issn>0304-4017</issn><issn>1873-2550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUcuK1UAQbUQZr6M_IApZiS6i1Uk_ks2ADr7gohtdN51OJbecJB27cwfux_ivdsxl3OmqijqPgnMYe8bhNQeu3kAJIhfA9ctavgKoeJmre2zHK13mhZRwn-3uKA_Zoxh_AIAApS_YRaVlWuod-7Wn_rBkI7ngo_PzKWvJ9pOPFDPfZe9sg5Fs1vjbdLBT-_dCPY402eyGJlwwZjRlywGzg8XRD6dxPqx6GxbqyJEdhlNidOgWTB7ezwcajnH7O68bTr0PfQI7HO2A2ULuJj5mDzo7RHxynpfs-4f3364_5fuvHz9fv93nTuhyyW3Lpeta4AXY0mlRobQAjay10EUtW11rBKyx4QolOl5ZB6oBISqhnHJteclebL5z8D-PGBczUnQ4DHZCf4xGV4UquNb_JXKZqFxUiSg24pprDNiZOdBow8lwMGt9Zu3GrN2YWpo_9RmVZM_P_sdmxPZOdO4r4U83vLPe2D5QNF_2tUryQibwagMxRXVLGEx0hJPDlkIK3rSe_v39N6_XtIc</recordid><startdate>1996</startdate><enddate>1996</enddate><creator>Guglielmone, A.A.</creator><creator>Gaido, A.B.</creator><creator>Mangold, A.J.</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>1996</creationdate><title>Light microscopy diagnosis of Babesia bovis and Babesia bigemina kinetes in the haemolymph of artificially infected Boophilus microplus engorged female ticks</title><author>Guglielmone, A.A. ; Gaido, A.B. ; Mangold, A.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c473t-ad15cfd0120a3c748e5a00b59747295d797e0e9eb16e5ec18ac06b044846c6cd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Babesia - cytology</topic><topic>Babesia - isolation & purification</topic><topic>BABESIA BIGEMINA</topic><topic>BABESIA BOVIS</topic><topic>Babesia bovis - cytology</topic><topic>Babesia bovis - growth & development</topic><topic>Babesia bovis - isolation & purification</topic><topic>BOOPHILUS MICROPLUS</topic><topic>CALVES</topic><topic>Cattle</topic><topic>DIAGNOSIS</topic><topic>Diagnosis-Protozoa</topic><topic>DIAGNOSTIC</topic><topic>DIAGNOSTICO</topic><topic>Female</topic><topic>HAEMOLYMPH</topic><topic>HEMOLINFA</topic><topic>Hemolymph - parasitology</topic><topic>HEMOLYMPHE</topic><topic>Ixodidae</topic><topic>Microscopy - methods</topic><topic>Species Specificity</topic><topic>TERNERO</topic><topic>Ticks - parasitology</topic><topic>VEAU</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guglielmone, A.A.</creatorcontrib><creatorcontrib>Gaido, A.B.</creatorcontrib><creatorcontrib>Mangold, A.J.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guglielmone, A.A.</au><au>Gaido, A.B.</au><au>Mangold, A.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Light microscopy diagnosis of Babesia bovis and Babesia bigemina kinetes in the haemolymph of artificially infected Boophilus microplus engorged female ticks</atitle><jtitle>Veterinary parasitology</jtitle><addtitle>Vet Parasitol</addtitle><date>1996</date><risdate>1996</risdate><volume>61</volume><issue>1</issue><spage>15</spage><epage>20</epage><pages>15-20</pages><issn>0304-4017</issn><eissn>1873-2550</eissn><abstract>The length, width and position of the nucleus of
Babesia bovis and
Babesia bigemina kinetes from the haemolymph of
Boophilus microplus engorged female ticks were recorded. Additionally, the shape of
Babesia bovis kinetes were registered as curved, semi-curved or straight. To this aim
Boophilus microplus tick larvae from a colony free of
Babesia were fed on splenectomised calves artificially infected with either
Babesia bovis or
Babesia bigemina pathogenic strains. Six engorged female ticks showing an infection of at least ten mature kinetes of
Babesia bovis in a sample of haemolymph 5 days after detachment were also monitored 7, 9 and 10 days after collection. The same procedure was followed with six engorged female ticks infected with
Babesia bigemina.
One hundred and twenty kinetes of each species of
Babesia were evaluated. The mean length ± standard deviation and ranges for
Babesia bovis kinetes were 14.30 ± 0.922
μm and 11.9−16.3
μm, while the corresponding measures for the kinetes of
Babesia bigemina were 11.27 ± 0.900
μm and 9.0−13.1
μm (
P < 0.001,
t-test). The width was 3.33 ± 0.315
μm, 2.6−4.0
μm for
Babesia bovis and 2.24 ± 0.287
μm, 1.5−2.8
μm for
Babesia bigemina kinetes (
P < 0.001). The most common position of the nucleus was central for both species of
Babesia. A total of 58% of
Babesia bovis kinetes showed the typical curved tail. No effect of time post-collection and individual host ticks in the kinete of
Babesia bigemina was found while an unexpected influence of individual host tick in the width of
Babesia bovis kinetes was detected (
P < 0.01, analysis of variance).
The overlap in the sizes of kinetes from both species of
Babesia makes it difficult to apply the results to ticks of unknown babesial infection status. This finding is further complicated by the intra-specific size variations of
Babesia kinetes from different geographical origins.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>8750679</pmid><doi>10.1016/0304-4017(95)00813-6</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0304-4017 |
| ispartof | Veterinary parasitology, 1996, Vol.61 (1), p.15-20 |
| issn | 0304-4017 1873-2550 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78262177 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Babesia - cytology Babesia - isolation & purification BABESIA BIGEMINA BABESIA BOVIS Babesia bovis - cytology Babesia bovis - growth & development Babesia bovis - isolation & purification BOOPHILUS MICROPLUS CALVES Cattle DIAGNOSIS Diagnosis-Protozoa DIAGNOSTIC DIAGNOSTICO Female HAEMOLYMPH HEMOLINFA Hemolymph - parasitology HEMOLYMPHE Ixodidae Microscopy - methods Species Specificity TERNERO Ticks - parasitology VEAU |
| title | Light microscopy diagnosis of Babesia bovis and Babesia bigemina kinetes in the haemolymph of artificially infected Boophilus microplus engorged female ticks |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T06%3A23%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Light%20microscopy%20diagnosis%20of%20Babesia%20bovis%20and%20Babesia%20bigemina%20kinetes%20in%20the%20haemolymph%20of%20artificially%20infected%20Boophilus%20microplus%20engorged%20female%20ticks&rft.jtitle=Veterinary%20parasitology&rft.au=Guglielmone,%20A.A.&rft.date=1996&rft.volume=61&rft.issue=1&rft.spage=15&rft.epage=20&rft.pages=15-20&rft.issn=0304-4017&rft.eissn=1873-2550&rft_id=info:doi/10.1016/0304-4017(95)00813-6&rft_dat=%3Cproquest_cross%3E15782148%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15782148&rft_id=info:pmid/8750679&rft_els_id=0304401795008136&rfr_iscdi=true |