Clinical application of autologous cultured epithelia for the treatment of burn wounds and burn scars
This report presents our experience with autologous cultured human epithelia grafting on burn wounds, burn scars, and skin-graft donor sites in seven patients. Dispersed epidermal cells were cultured with 3T3 cells treated with mitomycin C. After 2 to 3 weeks, cultured epithelia (total 350 to 2250 c...
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| Veröffentlicht in: | Plastic and reconstructive surgery (1963) 1988-07, Vol.82 (1), p.99-108 |
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| container_title | Plastic and reconstructive surgery (1963) |
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| creator | Kumagai, N Nishina, H Tanabe, H Hosaka, T Ishida, H Ogino, Y |
| description | This report presents our experience with autologous cultured human epithelia grafting on burn wounds, burn scars, and skin-graft donor sites in seven patients. Dispersed epidermal cells were cultured with 3T3 cells treated with mitomycin C. After 2 to 3 weeks, cultured epithelia (total 350 to 2250 cm2) were grafted to the wound. The results showed that cultured epithelia grafts did not take so completely compared to the meshed skin grafts used for the coverage of burn wounds. However, cultured grafts placed on aseptic wounds adhered well and showed good appearance. In the histologic findings, normal differentiation of epidermal cells was found. Cultured grafts were bordered from subepidermal granulative tissue with basement membrane. A rete ridge and the adnexal structures were absent in the specimens that adhered to the burn wounds. However, in the specimens that took on abraded wounds, a gently sloping rete ridge and elastic fibers were seen. The histologic findings showed structures resembling normal skin. |
| doi_str_mv | 10.1097/00006534-198882010-00017 |
| format | Article |
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Dispersed epidermal cells were cultured with 3T3 cells treated with mitomycin C. After 2 to 3 weeks, cultured epithelia (total 350 to 2250 cm2) were grafted to the wound. The results showed that cultured epithelia grafts did not take so completely compared to the meshed skin grafts used for the coverage of burn wounds. However, cultured grafts placed on aseptic wounds adhered well and showed good appearance. In the histologic findings, normal differentiation of epidermal cells was found. Cultured grafts were bordered from subepidermal granulative tissue with basement membrane. A rete ridge and the adnexal structures were absent in the specimens that adhered to the burn wounds. However, in the specimens that took on abraded wounds, a gently sloping rete ridge and elastic fibers were seen. 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Dispersed epidermal cells were cultured with 3T3 cells treated with mitomycin C. After 2 to 3 weeks, cultured epithelia (total 350 to 2250 cm2) were grafted to the wound. The results showed that cultured epithelia grafts did not take so completely compared to the meshed skin grafts used for the coverage of burn wounds. However, cultured grafts placed on aseptic wounds adhered well and showed good appearance. In the histologic findings, normal differentiation of epidermal cells was found. Cultured grafts were bordered from subepidermal granulative tissue with basement membrane. A rete ridge and the adnexal structures were absent in the specimens that adhered to the burn wounds. However, in the specimens that took on abraded wounds, a gently sloping rete ridge and elastic fibers were seen. The histologic findings showed structures resembling normal skin.</description><subject>Adult</subject><subject>Burns - surgery</subject><subject>Cells, Cultured</subject><subject>Child</subject><subject>Cicatrix - surgery</subject><subject>Epithelium - transplantation</subject><subject>Female</subject><subject>Humans</subject><subject>Male</subject><subject>Skin - cytology</subject><subject>Skin Transplantation</subject><subject>Transplantation, Autologous - methods</subject><subject>Wound Healing</subject><issn>0032-1052</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kMlOwzAQhn0AlVJ4BCSfuAW8JLZzRBWbVIkLnCPHnkCQEwfbEeLtcWjpXGb9ZzQfQpiSG0pqeUuyiYqXBa2VUoxQUuQKlSdoTQhnBSUVO0PnMX4uVS6qFVpxpmoi5BrB1vVjb7TDeppcDlLvR-w7rOfknX_3c8RmdmkOYDFMffoA12vc-YBziFMAnQYY0yJp5zDibz-PNmI92n0ejQ7xAp122kW4PPgNenu4f90-FbuXx-ft3a4wXIpUMFUJZbQBW1aCaKC8NK2QslW2kkzmruAAhCptTdkaazSU-RNWt4IK2jK-Qdf7vVPwXzPE1Ax9NOCcHiF_0kjFBC3FMqj2gyb4GAN0zRT6QYefhpJmodr8U22OVJs_qll6dbgxtwPYo_CAlP8CMB12GQ</recordid><startdate>19880701</startdate><enddate>19880701</enddate><creator>Kumagai, N</creator><creator>Nishina, H</creator><creator>Tanabe, H</creator><creator>Hosaka, T</creator><creator>Ishida, H</creator><creator>Ogino, Y</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19880701</creationdate><title>Clinical application of autologous cultured epithelia for the treatment of burn wounds and burn scars</title><author>Kumagai, N ; Nishina, H ; Tanabe, H ; Hosaka, T ; Ishida, H ; Ogino, Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c376t-28568caced4560ae134cb677b8d572785663ee018adc4bcdcae432829b6161b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Adult</topic><topic>Burns - surgery</topic><topic>Cells, Cultured</topic><topic>Child</topic><topic>Cicatrix - surgery</topic><topic>Epithelium - transplantation</topic><topic>Female</topic><topic>Humans</topic><topic>Male</topic><topic>Skin - cytology</topic><topic>Skin Transplantation</topic><topic>Transplantation, Autologous - methods</topic><topic>Wound Healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kumagai, N</creatorcontrib><creatorcontrib>Nishina, H</creatorcontrib><creatorcontrib>Tanabe, H</creatorcontrib><creatorcontrib>Hosaka, T</creatorcontrib><creatorcontrib>Ishida, H</creatorcontrib><creatorcontrib>Ogino, Y</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plastic and reconstructive surgery (1963)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kumagai, N</au><au>Nishina, H</au><au>Tanabe, H</au><au>Hosaka, T</au><au>Ishida, H</au><au>Ogino, Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Clinical application of autologous cultured epithelia for the treatment of burn wounds and burn scars</atitle><jtitle>Plastic and reconstructive surgery (1963)</jtitle><addtitle>Plast Reconstr Surg</addtitle><date>1988-07-01</date><risdate>1988</risdate><volume>82</volume><issue>1</issue><spage>99</spage><epage>108</epage><pages>99-108</pages><issn>0032-1052</issn><abstract>This report presents our experience with autologous cultured human epithelia grafting on burn wounds, burn scars, and skin-graft donor sites in seven patients. Dispersed epidermal cells were cultured with 3T3 cells treated with mitomycin C. After 2 to 3 weeks, cultured epithelia (total 350 to 2250 cm2) were grafted to the wound. The results showed that cultured epithelia grafts did not take so completely compared to the meshed skin grafts used for the coverage of burn wounds. However, cultured grafts placed on aseptic wounds adhered well and showed good appearance. In the histologic findings, normal differentiation of epidermal cells was found. Cultured grafts were bordered from subepidermal granulative tissue with basement membrane. A rete ridge and the adnexal structures were absent in the specimens that adhered to the burn wounds. However, in the specimens that took on abraded wounds, a gently sloping rete ridge and elastic fibers were seen. The histologic findings showed structures resembling normal skin.</abstract><cop>United States</cop><pmid>3289067</pmid><doi>10.1097/00006534-198882010-00017</doi><tpages>10</tpages></addata></record> |
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| ispartof | Plastic and reconstructive surgery (1963), 1988-07, Vol.82 (1), p.99-108 |
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| language | eng |
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| source | MEDLINE; Journals@Ovid Complete |
| subjects | Adult Burns - surgery Cells, Cultured Child Cicatrix - surgery Epithelium - transplantation Female Humans Male Skin - cytology Skin Transplantation Transplantation, Autologous - methods Wound Healing |
| title | Clinical application of autologous cultured epithelia for the treatment of burn wounds and burn scars |
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