Sex Differences in [3H]Nitrendipine Binding and Effects of Sex Steroid Hormones in Rat Cardiac and Cerebral Membranes

The sex differences and regulation by sex steroid hormones in calcium channels were studied by using [3H]nitrendipine binding to cardiac and cerebral membranes in 1 5-week old spontaneously hypertensive rats (SHRs). The maximal number of binding sites (Bmax) in the hippocampus of female SHRs increas...

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Veröffentlicht in:Japanese Journal of Pharmacology 1988, Vol.46 (2), p.117-125
Hauptverfasser: ISHII, Kenji, KANO, Takashi, ANDO, Joichi
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container_title Japanese Journal of Pharmacology
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creator ISHII, Kenji
KANO, Takashi
ANDO, Joichi
description The sex differences and regulation by sex steroid hormones in calcium channels were studied by using [3H]nitrendipine binding to cardiac and cerebral membranes in 1 5-week old spontaneously hypertensive rats (SHRs). The maximal number of binding sites (Bmax) in the hippocampus of female SHRs increased by 24.1% over that in male SHRs. In the females, the Bmax values in the cardiac, striatal, thalamic and hippocampal membranes from ovariectomized SHRs decreased by 34.7, 29.9, 29.3 and 26.9%, respectively, compared to normal SHRs. This phenomenon, except for the hippocampus, was inhibited by estradiol but not by testosterone. In the male, the Bmax values in cardiac and cerebral membranes showed almost no changes after orchidectomy or treatment with estradiol or testosterone. After gonadectomy, the Bmax values in the cardiac, striatal and thalamic membranes of females decreased by 30.2, 33.0 and 35.6%, respectively, compared to those in males. The changes in apparent dissociation constant (KD) values were less remarkable than those in the Bmax values. These findings suggest that sex differences exist in the calcium channels of the heart, striatum, thalamus and hippocampus, and they suggest that estradiol, but not testosterone, may play a part in the regulation of the calcium channels in female SHRs.
doi_str_mv 10.1016/S0021-5198(19)43326-X
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Drug treatments</topic><topic>Rats</topic><topic>Rats, Inbred SHR</topic><topic>Rats, Inbred WKY</topic><topic>Sex Factors</topic><topic>Thalamus - drug effects</topic><topic>Thalamus - metabolism</topic><topic>Thermodynamics</topic><toplevel>online_resources</toplevel><creatorcontrib>ISHII, Kenji</creatorcontrib><creatorcontrib>KANO, Takashi</creatorcontrib><creatorcontrib>ANDO, Joichi</creatorcontrib><creatorcontrib>Osaka Medical College</creatorcontrib><creatorcontrib>Department of Pharmacology</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Japanese Journal of Pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ISHII, Kenji</au><au>KANO, Takashi</au><au>ANDO, Joichi</au><aucorp>Osaka Medical College</aucorp><aucorp>Department of Pharmacology</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sex Differences in [3H]Nitrendipine Binding and Effects of Sex Steroid Hormones in Rat Cardiac and Cerebral Membranes</atitle><jtitle>Japanese Journal of Pharmacology</jtitle><addtitle>Jpn J Pharmacol</addtitle><date>1988</date><risdate>1988</risdate><volume>46</volume><issue>2</issue><spage>117</spage><epage>125</epage><pages>117-125</pages><issn>0021-5198</issn><eissn>1347-3506</eissn><coden>JJPAAZ</coden><abstract>The sex differences and regulation by sex steroid hormones in calcium channels were studied by using [3H]nitrendipine binding to cardiac and cerebral membranes in 1 5-week old spontaneously hypertensive rats (SHRs). 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ispartof Japanese Journal of Pharmacology, 1988, Vol.46 (2), p.117-125
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source J-STAGE Free; MEDLINE; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry
subjects Animals
Biological and medical sciences
Brain - drug effects
Brain - metabolism
Cardiovascular system
Corpus Striatum - drug effects
Corpus Striatum - metabolism
Female
Gonadal Steroid Hormones - pharmacology
Hippocampus - drug effects
Hippocampus - metabolism
In Vitro Techniques
Male
Medical sciences
Miscellaneous
Myocardium - metabolism
Nitrendipine - metabolism
Pharmacology. Drug treatments
Rats
Rats, Inbred SHR
Rats, Inbred WKY
Sex Factors
Thalamus - drug effects
Thalamus - metabolism
Thermodynamics
title Sex Differences in [3H]Nitrendipine Binding and Effects of Sex Steroid Hormones in Rat Cardiac and Cerebral Membranes
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