The expression in Escherichia coli of recombinant human platelet factor 4, a protein with immunoregulatory activity

In order to establish more firmly the immunoregulatory effect of platelet factor 4 (PF4) and develop a means to provide material for possible clinical use, the nucleotide sequence for PF4 was synthesized utilizing a ligation strategy of six duplexes ranging from 27 to 43 base pairs in length. The in...

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Veröffentlicht in:	The Journal of biological chemistry 1988-06, Vol.263 (18), p.8710-8715
Hauptverfasser:	Barone, A D, Ghrayeb, J, Hammerling, U, Zucker, M B, Thorbecke, G J
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Antibodies, Monoclonal Antigen-Antibody Complex - analysis Base Sequence Biological and medical sciences Biotechnology cloning Concanavalin A - pharmacology Erythrocytes - immunology Escherichia coli Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genes Genes, Synthetic Genetic engineering Genetic technics Humans immunosuppression Methods. Procedures. Technologies Molecular cloning oligonucleotides platelet factor 4 Platelet Factor 4 - genetics Platelet Factor 4 - immunology Platelet Factor 4 - isolation & purification Recombinant Proteins - immunology Recombinant Proteins - isolation & purification Sheep
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