Mercury and tetracycline resistance genes and flanking repeats associated with methicillin resistance on the chromosome of Staphylococcus aureus
Summary Sections of a cloned 27 kb segment of chromosomal DNA, associated with resistance to four antimicrobial agents in a clinical isolate of methicillin‐resistant Staphylococcus aureus (MRSA), were tested for their ability to determine resistance when transformed into a sensitive laboratory strai...
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| Veröffentlicht in: | Molecular microbiology 1988-03, Vol.2 (2), p.289-292 |
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| container_title | Molecular microbiology |
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| creator | Skinner, S. Inglis, B. Matthews, P. R. Stewart, P. R. |
| description | Summary
Sections of a cloned 27 kb segment of chromosomal DNA, associated with resistance to four antimicrobial agents in a clinical isolate of methicillin‐resistant Staphylococcus aureus (MRSA), were tested for their ability to determine resistance when transformed into a sensitive laboratory strain of S. aureus. This was achieved by inserting the sections into a newly constructed shuttle vector, amplifying the recombinant DNA In E. coli, and transforming protoplasts of the sensitive S. aureus strain. Two sections of the cloned DNA were found to determine resistance separately to mercuric ion and to tetracycline, in both S. aureus and Escherichia coli. |
| doi_str_mv | 10.1111/j.1365-2958.1988.tb00030.x |
| format | Article |
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Sections of a cloned 27 kb segment of chromosomal DNA, associated with resistance to four antimicrobial agents in a clinical isolate of methicillin‐resistant Staphylococcus aureus (MRSA), were tested for their ability to determine resistance when transformed into a sensitive laboratory strain of S. aureus. This was achieved by inserting the sections into a newly constructed shuttle vector, amplifying the recombinant DNA In E. coli, and transforming protoplasts of the sensitive S. aureus strain. Two sections of the cloned DNA were found to determine resistance separately to mercuric ion and to tetracycline, in both S. aureus and Escherichia coli.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1111/j.1365-2958.1988.tb00030.x</identifier><identifier>PMID: 2837617</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Bacteriology ; Biological and medical sciences ; Chromosomes, Bacterial - physiology ; Cloning, Molecular ; DNA Restriction Enzymes ; Drug Resistance, Microbial - genetics ; Fundamental and applied biological sciences. Psychology ; Genes, Bacterial ; Genetics ; Mercury - pharmacology ; Methicillin - pharmacology ; Microbiology ; Nucleotide Mapping ; Repetitive Sequences, Nucleic Acid ; Staphylococcus aureus - drug effects ; Staphylococcus aureus - genetics ; Tetracycline Resistance - genetics</subject><ispartof>Molecular microbiology, 1988-03, Vol.2 (2), p.289-292</ispartof><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3989-b1583a6a268b67f5232a687da4e779fc3918559c689685be3febe426a7da11493</citedby><cites>FETCH-LOGICAL-c3989-b1583a6a268b67f5232a687da4e779fc3918559c689685be3febe426a7da11493</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2958.1988.tb00030.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2958.1988.tb00030.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7002905$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2837617$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Skinner, S.</creatorcontrib><creatorcontrib>Inglis, B.</creatorcontrib><creatorcontrib>Matthews, P. R.</creatorcontrib><creatorcontrib>Stewart, P. R.</creatorcontrib><title>Mercury and tetracycline resistance genes and flanking repeats associated with methicillin resistance on the chromosome of Staphylococcus aureus</title><title>Molecular microbiology</title><addtitle>Mol Microbiol</addtitle><description>Summary
Sections of a cloned 27 kb segment of chromosomal DNA, associated with resistance to four antimicrobial agents in a clinical isolate of methicillin‐resistant Staphylococcus aureus (MRSA), were tested for their ability to determine resistance when transformed into a sensitive laboratory strain of S. aureus. This was achieved by inserting the sections into a newly constructed shuttle vector, amplifying the recombinant DNA In E. coli, and transforming protoplasts of the sensitive S. aureus strain. Two sections of the cloned DNA were found to determine resistance separately to mercuric ion and to tetracycline, in both S. aureus and Escherichia coli.</description><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Chromosomes, Bacterial - physiology</subject><subject>Cloning, Molecular</subject><subject>DNA Restriction Enzymes</subject><subject>Drug Resistance, Microbial - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Bacterial</subject><subject>Genetics</subject><subject>Mercury - pharmacology</subject><subject>Methicillin - pharmacology</subject><subject>Microbiology</subject><subject>Nucleotide Mapping</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Staphylococcus aureus - genetics</subject><subject>Tetracycline Resistance - genetics</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkc1q3DAUhUVpSadpH6FgSunOjn5GstRFIYT-BDJk0Ra6E7LmOtbUtqaSTOK36CNH0zFDttVGcM93z5XORegdwRXJ52JXESZ4SRWXFVFSVqnBGDNcPTxDq5P0HK2w4rhkkv56iV7FuMOYMCzYGTqjktWC1Cv0dwPBTmEuzLgtEqRg7Gx7N0IRILqYzGihuIMR4j-i7c342413Wd2DSbkYo7fOJNgW9y51xQCpc9b12eKpgx-L1EFhu-AHH_2QK23xPZl9N_feemunbDUFmOJr9KI1fYQ3y32Ofn75_OPqW3lz-_X66vKmtExJVTaES2aEoUI2om45ZdQIWW_NGupatRkiknNlhVRC8gZYCw2sqTAZIWSt2Dn6cPTdB_9ngpj04KKFPn8Q_BR1LSlXQrEMfjyCNvgYA7R6H9xgwqwJ1od16J0-ZK4PmevDOvSyDv2Qm98uU6ZmgO2pdck_6-8X3URr-jbktFw8YTXGVGGesU9H7N71MP_HA_Rmc02lYo9R_qsX</recordid><startdate>198803</startdate><enddate>198803</enddate><creator>Skinner, S.</creator><creator>Inglis, B.</creator><creator>Matthews, P. R.</creator><creator>Stewart, P. R.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198803</creationdate><title>Mercury and tetracycline resistance genes and flanking repeats associated with methicillin resistance on the chromosome of Staphylococcus aureus</title><author>Skinner, S. ; Inglis, B. ; Matthews, P. R. ; Stewart, P. R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3989-b1583a6a268b67f5232a687da4e779fc3918559c689685be3febe426a7da11493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Chromosomes, Bacterial - physiology</topic><topic>Cloning, Molecular</topic><topic>DNA Restriction Enzymes</topic><topic>Drug Resistance, Microbial - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Bacterial</topic><topic>Genetics</topic><topic>Mercury - pharmacology</topic><topic>Methicillin - pharmacology</topic><topic>Microbiology</topic><topic>Nucleotide Mapping</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Staphylococcus aureus - genetics</topic><topic>Tetracycline Resistance - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Skinner, S.</creatorcontrib><creatorcontrib>Inglis, B.</creatorcontrib><creatorcontrib>Matthews, P. R.</creatorcontrib><creatorcontrib>Stewart, P. R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Skinner, S.</au><au>Inglis, B.</au><au>Matthews, P. R.</au><au>Stewart, P. R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mercury and tetracycline resistance genes and flanking repeats associated with methicillin resistance on the chromosome of Staphylococcus aureus</atitle><jtitle>Molecular microbiology</jtitle><addtitle>Mol Microbiol</addtitle><date>1988-03</date><risdate>1988</risdate><volume>2</volume><issue>2</issue><spage>289</spage><epage>292</epage><pages>289-292</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>Summary
Sections of a cloned 27 kb segment of chromosomal DNA, associated with resistance to four antimicrobial agents in a clinical isolate of methicillin‐resistant Staphylococcus aureus (MRSA), were tested for their ability to determine resistance when transformed into a sensitive laboratory strain of S. aureus. This was achieved by inserting the sections into a newly constructed shuttle vector, amplifying the recombinant DNA In E. coli, and transforming protoplasts of the sensitive S. aureus strain. Two sections of the cloned DNA were found to determine resistance separately to mercuric ion and to tetracycline, in both S. aureus and Escherichia coli.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>2837617</pmid><doi>10.1111/j.1365-2958.1988.tb00030.x</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0950-382X |
| ispartof | Molecular microbiology, 1988-03, Vol.2 (2), p.289-292 |
| issn | 0950-382X 1365-2958 |
| language | eng |
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| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Bacteriology Biological and medical sciences Chromosomes, Bacterial - physiology Cloning, Molecular DNA Restriction Enzymes Drug Resistance, Microbial - genetics Fundamental and applied biological sciences. Psychology Genes, Bacterial Genetics Mercury - pharmacology Methicillin - pharmacology Microbiology Nucleotide Mapping Repetitive Sequences, Nucleic Acid Staphylococcus aureus - drug effects Staphylococcus aureus - genetics Tetracycline Resistance - genetics |
| title | Mercury and tetracycline resistance genes and flanking repeats associated with methicillin resistance on the chromosome of Staphylococcus aureus |
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