A method for the preparation of mononuclear cells devoid of platelet contamination and its application to the evaluation of putative α-receptors in normal and asthmatic subjects

Receptor studies of human mononuclear leukocytes (MNLs) are complicated by the presence of contaminating platelets which have common receptors. A method was devised to produce MNLs free of platelets (< 1%) and consists of sequential Ficoll-Hypaque gradients, a BSA gradient and a washing step. Lac...

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Veröffentlicht in:	Journal of immunological methods 1988-06, Vol.110 (2), p.193-202
Hauptverfasser:	Lad, P.M., Easton, J., Niedzin, H., Olson, C.V., Goldberg, B.J., Kaplan, M., Parkman, R., Learn, D.B., Reisinger, D.M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenylyl Cyclases - blood Adult Asthma Asthma - blood Biological and medical sciences Blood Platelets - metabolism Cell Membrane - metabolism Cell Separation - methods Centrifugation, Density Gradient Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Kinetics Lymphocytes - metabolism Molecular immunology Mononuclear cell Receptors, Adrenergic, alpha - analysis Serum Albumin, Bovine Techniques Yohimbine - blood α-Receptor
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