The human osteoclast precursor circulates in the monocyte fraction
The osteoclast is known to be formed by fusion of circulating mononuclear precursor cells of haematopoietic origin. The precise nature of these circulating cells and, in particular, their relation to monocytes is unknown. We have developed an in vitro system of human osteoclast formation whereby hum...
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| Veröffentlicht in: | Endocrinology (Philadelphia) 1996-09, Vol.137 (9), p.4058-4060 |
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| creator | Fujikawa, Y Quinn, J M Sabokbar, A McGee, J O Athanasou, N A |
| description | The osteoclast is known to be formed by fusion of circulating mononuclear precursor cells of haematopoietic origin. The precise nature of these circulating cells and, in particular, their relation to monocytes is unknown. We have developed an in vitro system of human osteoclast formation whereby human monocytes [CD14, CD11a, CD11b and HLA-DR positive, and tartrate-resistant acid phosphatase (TRAP), calcitonin receptor (CTR), vitronectin receptor (VNR) negative] were isolated and cocultured for up to 21 days with UMR106 rat osteoblast-like cells or ST2 mouse preadipocytic bone marrow stromal cells in the presence of 1 alpha, 25 dihydroxyvitamin D3 (1,25(OH)2D3) and macrophage colony stimulating factor (M-CSF). Numerous TRAP, VNR and CTR positive multinucleated cells, capable of extensive lacunar bone resorption, formed in these cocultures; the absolute requirements for this to occur were contact with the above bone stromal cells, 1,25(OH)2D3, and M-CSF. These results show that the human mononuclear osteoclast precursor circulates in the monocyte fraction and exhibits a monocyte phenotype, acquiring osteoclast phenotypic features in the process of differentiation into mature functional osteoclasts. |
| doi_str_mv | 10.1210/en.137.9.4058 |
| format | Article |
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The precise nature of these circulating cells and, in particular, their relation to monocytes is unknown. We have developed an in vitro system of human osteoclast formation whereby human monocytes [CD14, CD11a, CD11b and HLA-DR positive, and tartrate-resistant acid phosphatase (TRAP), calcitonin receptor (CTR), vitronectin receptor (VNR) negative] were isolated and cocultured for up to 21 days with UMR106 rat osteoblast-like cells or ST2 mouse preadipocytic bone marrow stromal cells in the presence of 1 alpha, 25 dihydroxyvitamin D3 (1,25(OH)2D3) and macrophage colony stimulating factor (M-CSF). Numerous TRAP, VNR and CTR positive multinucleated cells, capable of extensive lacunar bone resorption, formed in these cocultures; the absolute requirements for this to occur were contact with the above bone stromal cells, 1,25(OH)2D3, and M-CSF. These results show that the human mononuclear osteoclast precursor circulates in the monocyte fraction and exhibits a monocyte phenotype, acquiring osteoclast phenotypic features in the process of differentiation into mature functional osteoclasts.</description><identifier>ISSN: 0013-7227</identifier><identifier>EISSN: 1945-7170</identifier><identifier>DOI: 10.1210/en.137.9.4058</identifier><identifier>PMID: 8756585</identifier><language>eng</language><publisher>United States: Endocrine Society</publisher><subject>Acid phosphatase ; Acid phosphatase (tartrate-resistant) ; Acid Phosphatase - metabolism ; Acid resistance ; Animals ; Bone marrow ; Bone Marrow - physiology ; Bone Marrow Cells ; Bone resorption ; Calcitonin ; Calcitriol ; Calcitriol - pharmacology ; CD11a antigen ; CD11b antigen ; CD14 antigen ; Cell Differentiation ; Cell fusion ; Coculture Techniques ; Colony-stimulating factor ; Humans ; Isoenzymes - metabolism ; Macrophage colony-stimulating factor ; Macrophage Colony-Stimulating Factor - pharmacology ; Macrophages ; Mice ; Monocytes ; Monocytes - cytology ; Monocytes - physiology ; Osteoblasts - physiology ; Osteoclastogenesis ; Osteoclasts ; Osteoclasts - physiology ; Phenotypes ; Precursors ; Rats ; Receptors ; Receptors, Calcitonin - metabolism ; Receptors, Vitronectin - metabolism ; Stem Cells - physiology ; Stromal cells ; Stromal Cells - cytology ; Stromal Cells - physiology ; Tartrate-Resistant Acid Phosphatase ; Vitamin D3 ; Vitronectin</subject><ispartof>Endocrinology (Philadelphia), 1996-09, Vol.137 (9), p.4058-4060</ispartof><rights>Copyright © 1996 by The Endocrine Society 1996</rights><rights>Copyright © 1996 by The Endocrine Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c379t-7d2291d44008bab22b437e20fd6384befd5e80c05cae2cc0347a90c89822a353</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8756585$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fujikawa, Y</creatorcontrib><creatorcontrib>Quinn, J M</creatorcontrib><creatorcontrib>Sabokbar, A</creatorcontrib><creatorcontrib>McGee, J O</creatorcontrib><creatorcontrib>Athanasou, N A</creatorcontrib><title>The human osteoclast precursor circulates in the monocyte fraction</title><title>Endocrinology (Philadelphia)</title><addtitle>Endocrinology</addtitle><description>The osteoclast is known to be formed by fusion of circulating mononuclear precursor cells of haematopoietic origin. The precise nature of these circulating cells and, in particular, their relation to monocytes is unknown. We have developed an in vitro system of human osteoclast formation whereby human monocytes [CD14, CD11a, CD11b and HLA-DR positive, and tartrate-resistant acid phosphatase (TRAP), calcitonin receptor (CTR), vitronectin receptor (VNR) negative] were isolated and cocultured for up to 21 days with UMR106 rat osteoblast-like cells or ST2 mouse preadipocytic bone marrow stromal cells in the presence of 1 alpha, 25 dihydroxyvitamin D3 (1,25(OH)2D3) and macrophage colony stimulating factor (M-CSF). Numerous TRAP, VNR and CTR positive multinucleated cells, capable of extensive lacunar bone resorption, formed in these cocultures; the absolute requirements for this to occur were contact with the above bone stromal cells, 1,25(OH)2D3, and M-CSF. These results show that the human mononuclear osteoclast precursor circulates in the monocyte fraction and exhibits a monocyte phenotype, acquiring osteoclast phenotypic features in the process of differentiation into mature functional osteoclasts.</description><subject>Acid phosphatase</subject><subject>Acid phosphatase (tartrate-resistant)</subject><subject>Acid Phosphatase - metabolism</subject><subject>Acid resistance</subject><subject>Animals</subject><subject>Bone marrow</subject><subject>Bone Marrow - physiology</subject><subject>Bone Marrow Cells</subject><subject>Bone resorption</subject><subject>Calcitonin</subject><subject>Calcitriol</subject><subject>Calcitriol - pharmacology</subject><subject>CD11a antigen</subject><subject>CD11b antigen</subject><subject>CD14 antigen</subject><subject>Cell Differentiation</subject><subject>Cell fusion</subject><subject>Coculture Techniques</subject><subject>Colony-stimulating factor</subject><subject>Humans</subject><subject>Isoenzymes - metabolism</subject><subject>Macrophage colony-stimulating factor</subject><subject>Macrophage Colony-Stimulating Factor - pharmacology</subject><subject>Macrophages</subject><subject>Mice</subject><subject>Monocytes</subject><subject>Monocytes - cytology</subject><subject>Monocytes - physiology</subject><subject>Osteoblasts - physiology</subject><subject>Osteoclastogenesis</subject><subject>Osteoclasts</subject><subject>Osteoclasts - physiology</subject><subject>Phenotypes</subject><subject>Precursors</subject><subject>Rats</subject><subject>Receptors</subject><subject>Receptors, Calcitonin - metabolism</subject><subject>Receptors, Vitronectin - metabolism</subject><subject>Stem Cells - physiology</subject><subject>Stromal cells</subject><subject>Stromal Cells - cytology</subject><subject>Stromal Cells - physiology</subject><subject>Tartrate-Resistant Acid Phosphatase</subject><subject>Vitamin D3</subject><subject>Vitronectin</subject><issn>0013-7227</issn><issn>1945-7170</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkT1rwzAQhkVpSdO0Y8eCoVC62D19RdLYhn5BoEt2IcsycbAtV5KH_Ps6JHTo0uk43oeDex-EbjEUmGB4cn2BqShUwYDLMzTHivFcYAHnaA6AaS4IEZfoKsbdtDLG6AzNpOBLLvkcvWy2LtuOnekzH5PztjUxZUNwdgzRh8w2wY6tSS5mTZ-lCe587-0-uawOxqbG99foojZtdDenuUCbt9fN6iNff71_rp7XuaVCpVxUhChcMQYgS1MSUjIqHIG6WlLJSldX3EmwwK1xxFqgTBgFVipJiKGcLtDD8ewQ_PfoYtJdE61rW9M7P0YtJOFEKfgXxFwINVU3gfd_wJ0fQz_9oCmmwBQQLCbq7kSNZecqPYSmM2GvTxVO-eMx9-PwG2LQBzna9XqSo5U-yKE_-qZ-CA</recordid><startdate>19960901</startdate><enddate>19960901</enddate><creator>Fujikawa, Y</creator><creator>Quinn, J M</creator><creator>Sabokbar, A</creator><creator>McGee, J O</creator><creator>Athanasou, N A</creator><general>Endocrine Society</general><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19960901</creationdate><title>The human osteoclast precursor circulates in the monocyte fraction</title><author>Fujikawa, Y ; Quinn, J M ; Sabokbar, A ; McGee, J O ; Athanasou, N A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c379t-7d2291d44008bab22b437e20fd6384befd5e80c05cae2cc0347a90c89822a353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Acid phosphatase</topic><topic>Acid phosphatase (tartrate-resistant)</topic><topic>Acid Phosphatase - metabolism</topic><topic>Acid resistance</topic><topic>Animals</topic><topic>Bone marrow</topic><topic>Bone Marrow - physiology</topic><topic>Bone Marrow Cells</topic><topic>Bone resorption</topic><topic>Calcitonin</topic><topic>Calcitriol</topic><topic>Calcitriol - pharmacology</topic><topic>CD11a antigen</topic><topic>CD11b antigen</topic><topic>CD14 antigen</topic><topic>Cell Differentiation</topic><topic>Cell fusion</topic><topic>Coculture Techniques</topic><topic>Colony-stimulating factor</topic><topic>Humans</topic><topic>Isoenzymes - metabolism</topic><topic>Macrophage colony-stimulating factor</topic><topic>Macrophage Colony-Stimulating Factor - pharmacology</topic><topic>Macrophages</topic><topic>Mice</topic><topic>Monocytes</topic><topic>Monocytes - cytology</topic><topic>Monocytes - physiology</topic><topic>Osteoblasts - physiology</topic><topic>Osteoclastogenesis</topic><topic>Osteoclasts</topic><topic>Osteoclasts - physiology</topic><topic>Phenotypes</topic><topic>Precursors</topic><topic>Rats</topic><topic>Receptors</topic><topic>Receptors, Calcitonin - metabolism</topic><topic>Receptors, Vitronectin - metabolism</topic><topic>Stem Cells - physiology</topic><topic>Stromal cells</topic><topic>Stromal Cells - cytology</topic><topic>Stromal Cells - physiology</topic><topic>Tartrate-Resistant Acid Phosphatase</topic><topic>Vitamin D3</topic><topic>Vitronectin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fujikawa, Y</creatorcontrib><creatorcontrib>Quinn, J M</creatorcontrib><creatorcontrib>Sabokbar, A</creatorcontrib><creatorcontrib>McGee, J O</creatorcontrib><creatorcontrib>Athanasou, N A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Endocrinology (Philadelphia)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fujikawa, Y</au><au>Quinn, J M</au><au>Sabokbar, A</au><au>McGee, J O</au><au>Athanasou, N A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The human osteoclast precursor circulates in the monocyte fraction</atitle><jtitle>Endocrinology (Philadelphia)</jtitle><addtitle>Endocrinology</addtitle><date>1996-09-01</date><risdate>1996</risdate><volume>137</volume><issue>9</issue><spage>4058</spage><epage>4060</epage><pages>4058-4060</pages><issn>0013-7227</issn><eissn>1945-7170</eissn><abstract>The osteoclast is known to be formed by fusion of circulating mononuclear precursor cells of haematopoietic origin. The precise nature of these circulating cells and, in particular, their relation to monocytes is unknown. We have developed an in vitro system of human osteoclast formation whereby human monocytes [CD14, CD11a, CD11b and HLA-DR positive, and tartrate-resistant acid phosphatase (TRAP), calcitonin receptor (CTR), vitronectin receptor (VNR) negative] were isolated and cocultured for up to 21 days with UMR106 rat osteoblast-like cells or ST2 mouse preadipocytic bone marrow stromal cells in the presence of 1 alpha, 25 dihydroxyvitamin D3 (1,25(OH)2D3) and macrophage colony stimulating factor (M-CSF). Numerous TRAP, VNR and CTR positive multinucleated cells, capable of extensive lacunar bone resorption, formed in these cocultures; the absolute requirements for this to occur were contact with the above bone stromal cells, 1,25(OH)2D3, and M-CSF. These results show that the human mononuclear osteoclast precursor circulates in the monocyte fraction and exhibits a monocyte phenotype, acquiring osteoclast phenotypic features in the process of differentiation into mature functional osteoclasts.</abstract><cop>United States</cop><pub>Endocrine Society</pub><pmid>8756585</pmid><doi>10.1210/en.137.9.4058</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Endocrinology (Philadelphia), 1996-09, Vol.137 (9), p.4058-4060 |
| issn | 0013-7227 1945-7170 |
| language | eng |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Acid phosphatase Acid phosphatase (tartrate-resistant) Acid Phosphatase - metabolism Acid resistance Animals Bone marrow Bone Marrow - physiology Bone Marrow Cells Bone resorption Calcitonin Calcitriol Calcitriol - pharmacology CD11a antigen CD11b antigen CD14 antigen Cell Differentiation Cell fusion Coculture Techniques Colony-stimulating factor Humans Isoenzymes - metabolism Macrophage colony-stimulating factor Macrophage Colony-Stimulating Factor - pharmacology Macrophages Mice Monocytes Monocytes - cytology Monocytes - physiology Osteoblasts - physiology Osteoclastogenesis Osteoclasts Osteoclasts - physiology Phenotypes Precursors Rats Receptors Receptors, Calcitonin - metabolism Receptors, Vitronectin - metabolism Stem Cells - physiology Stromal cells Stromal Cells - cytology Stromal Cells - physiology Tartrate-Resistant Acid Phosphatase Vitamin D3 Vitronectin |
| title | The human osteoclast precursor circulates in the monocyte fraction |
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