Molecular cloning and nucleotide sequence of the cDNA for human liver glutamate dehydrogenase precursor
Two cDNA clones (λGDHh1 and λGDHn61) for glutamate dehydrogenase (GDH) were isolated from a human liver cDNA library in λtll. The clone, λGDHh1, was isolated from the library using a synthetic 45mer oligodeoxy-ribonucleotide, the sequence of which was derived from the known amino acid sequence near...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1988-05, Vol.152 (3), p.1395-1400 |
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| creator | Amuro, Naoki Yamaura, Mayumi Goto, Yoshitaka Okazaki, Taro |
| description | Two cDNA clones (λGDHh1 and λGDHn61) for glutamate dehydrogenase (GDH) were isolated from a human liver cDNA library in λtll. The clone, λGDHh1, was isolated from the library using a synthetic 45mer oligodeoxy-ribonucleotide, the sequence of which was derived from the known amino acid sequence near the NH
2-terminus of human liver GDH. Subsequently, λGDHn61 was isolated from the same library using λGDHh1 as a probe. The inserts of both clones contained an overlapping cDNA sequence for human liver GDH, consisting of a 5′-untranslated region of 70 bp, an open reading frace of 1677 bp, a 3′-untranslated region of 1262 bp and a 15 base poly(A) tract. The predicted amino acid sequence revealed that the human liver GDH precursor consisted of a total of 558 amino acid residues including the NH
2-terminal presequence of 53 amino acids. The sequence deduced for the mature enzyme showed 94% homology to the previously reported amino acid sequence of human liver GDH. |
| doi_str_mv | 10.1016/S0006-291X(88)80440-6 |
| format | Article |
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2-terminus of human liver GDH. Subsequently, λGDHn61 was isolated from the same library using λGDHh1 as a probe. The inserts of both clones contained an overlapping cDNA sequence for human liver GDH, consisting of a 5′-untranslated region of 70 bp, an open reading frace of 1677 bp, a 3′-untranslated region of 1262 bp and a 15 base poly(A) tract. The predicted amino acid sequence revealed that the human liver GDH precursor consisted of a total of 558 amino acid residues including the NH
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2-terminus of human liver GDH. Subsequently, λGDHn61 was isolated from the same library using λGDHh1 as a probe. The inserts of both clones contained an overlapping cDNA sequence for human liver GDH, consisting of a 5′-untranslated region of 70 bp, an open reading frace of 1677 bp, a 3′-untranslated region of 1262 bp and a 15 base poly(A) tract. The predicted amino acid sequence revealed that the human liver GDH precursor consisted of a total of 558 amino acid residues including the NH
2-terminal presequence of 53 amino acids. The sequence deduced for the mature enzyme showed 94% homology to the previously reported amino acid sequence of human liver GDH.</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>DNA - analysis</subject><subject>Enzyme Precursors - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes. Genome</subject><subject>Glutamate Dehydrogenase - genetics</subject><subject>Humans</subject><subject>Liver - enzymology</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v3CAQxVHVKN2k_QiROFRVc3AyYIzhVEXpXylpD22l3hAL410qbLZgR8q3r51d7TVz4fB-zDy9R8gFgysGTF7_BABZcc3-vFfqUoEQUMkXZMVAQ8UZiJdkdURekbNS_gIwJqQ-Jad13S6zIpv7FNFN0WbqYhrCsKF28HSYXMQ0Bo-04L8JB4c0dXTcInUfv9_QLmW6nXo70BgeMNNNnEbb2xGpx-2jz2mDgy1Id3lenkvKr8lJZ2PBN4f3nPz-_OnX7dfq7seXb7c3d5UTHMZq3bSO1aKTVmiwdu06LmvlUQqnG8E7xda81a2VAM2sWWDaC6wbzWWrpZD1OXm337vLafZdRtOH4jBGO2CaimkVF6oR4lmQNTU0ki9gswddTqVk7Mwuh97mR8PALE2YpybMErNRyjw1YRYnF4cD07pHf_x1iH7W3x50W5yNXbaDC-WItTXnSusZ-7DHcE7tIWA2xYWlDx_mbEfjU3jGyH_a1aWL</recordid><startdate>19880516</startdate><enddate>19880516</enddate><creator>Amuro, Naoki</creator><creator>Yamaura, Mayumi</creator><creator>Goto, Yoshitaka</creator><creator>Okazaki, Taro</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19880516</creationdate><title>Molecular cloning and nucleotide sequence of the cDNA for human liver glutamate dehydrogenase precursor</title><author>Amuro, Naoki ; Yamaura, Mayumi ; Goto, Yoshitaka ; Okazaki, Taro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-b57c134f6a490aabcf2638de64c9542f81b2797a6005cf2a019d4e35926796463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cloning, Molecular</topic><topic>DNA - analysis</topic><topic>Enzyme Precursors - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes. Genome</topic><topic>Glutamate Dehydrogenase - genetics</topic><topic>Humans</topic><topic>Liver - enzymology</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Amuro, Naoki</creatorcontrib><creatorcontrib>Yamaura, Mayumi</creatorcontrib><creatorcontrib>Goto, Yoshitaka</creatorcontrib><creatorcontrib>Okazaki, Taro</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Amuro, Naoki</au><au>Yamaura, Mayumi</au><au>Goto, Yoshitaka</au><au>Okazaki, Taro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning and nucleotide sequence of the cDNA for human liver glutamate dehydrogenase precursor</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1988-05-16</date><risdate>1988</risdate><volume>152</volume><issue>3</issue><spage>1395</spage><epage>1400</epage><pages>1395-1400</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>Two cDNA clones (λGDHh1 and λGDHn61) for glutamate dehydrogenase (GDH) were isolated from a human liver cDNA library in λtll. The clone, λGDHh1, was isolated from the library using a synthetic 45mer oligodeoxy-ribonucleotide, the sequence of which was derived from the known amino acid sequence near the NH
2-terminus of human liver GDH. Subsequently, λGDHn61 was isolated from the same library using λGDHh1 as a probe. The inserts of both clones contained an overlapping cDNA sequence for human liver GDH, consisting of a 5′-untranslated region of 70 bp, an open reading frace of 1677 bp, a 3′-untranslated region of 1262 bp and a 15 base poly(A) tract. The predicted amino acid sequence revealed that the human liver GDH precursor consisted of a total of 558 amino acid residues including the NH
2-terminal presequence of 53 amino acids. The sequence deduced for the mature enzyme showed 94% homology to the previously reported amino acid sequence of human liver GDH.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>3377777</pmid><doi>10.1016/S0006-291X(88)80440-6</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1988-05, Vol.152 (3), p.1395-1400 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Amino Acid Sequence Base Sequence Biological and medical sciences Cloning, Molecular DNA - analysis Enzyme Precursors - genetics Fundamental and applied biological sciences. Psychology Genes. Genome Glutamate Dehydrogenase - genetics Humans Liver - enzymology Molecular and cellular biology Molecular genetics Molecular Sequence Data |
| title | Molecular cloning and nucleotide sequence of the cDNA for human liver glutamate dehydrogenase precursor |
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