Quantification of apoptotic cells with fluorescein isothiocyanate‐labeled annexin V in chinese hamster ovary cell cultures treated with cisplatin

Plasma membrane binding of annexin V was used to detect and quantitate apoptotic cells induced by cytotoxic drug treatment in epithelial cell cultures. Chinese hamster ovary (CHO) cells were incubated for 2 h with the ID90 concentration of Cisplatin (20 μM), and 24, 48, 72, and 96 h later the unfixe...

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Veröffentlicht in:	Cytometry (New York, N.Y.) N.Y.), 1996-06, Vol.24 (2), p.123-130
Hauptverfasser:	Boersma, Antonius W. M., Nooter, Kees, Oostrum, Robert G., Stoter, Gerrit
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Annexin A5 - metabolism annexin V Apoptosis - physiology CHO Cells Cisplatin - pharmacology Cisplatin‐induced apoptosis Cricetinae Flow Cytometry - methods Fluorescein-5-isothiocyanate - chemistry Fluorescent Dyes - chemistry quantification of early and late apoptotic cells by flow cytometry
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