Differentiation of yeast‐like fungi using the commercial Auxacolor system
Technical factors preclude the use of conventional fermentation and assimilation procedures for the routine laboratory differentiation of yeast‐like fungal isolates. Commercial biochemical differentiation systems are therefore used to remedy this situation. The predictive accuracy of three commercia...
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| Veröffentlicht in: | Mycoses 1996-03, Vol.39 (3‐4), p.135-140 |
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| creator | Hantschke, D. |
| description | Technical factors preclude the use of conventional fermentation and assimilation procedures for the routine laboratory differentiation of yeast‐like fungal isolates. Commercial biochemical differentiation systems are therefore used to remedy this situation. The predictive accuracy of three commercially available differentiation systems was tested using 120 yeast‐like fungal isolates. The morphological characteristics of these fungal strains were identical in the case of all three differentiation systems. The fungal isolates were correctly identified in 98.3% of cases with Auxacolor, in 90.8% of cases with API 20C AUX and in 76.7% of cases with the Mycotube system. The advantages and disadvantages of these biochemical differentiation systems are discussed.
Zusammenfassung. Mit den klassischen Methoden der Fermentation und der Assimilation ist es aus arbeitstechnischen Gründen nicht möglich, in Routinelaboratorien die anfallenden Hefepilzisolate zu differenzieren. Aus diesen Gründen werden als Hilfsmittel kommerzielle biochemische Differenzierungssysteme eingesetzt. Drei dieser käuflich zu erwerbenden Bunten Reihen wurden mit 120 Sproßpilzisolaten auf ihre Aussagefähigkeit überprüft. Die morphologischen Merkmale dieser Pilzstämme waren für alle drei Differenzierungssysteme einheitlich. Mit Auxacolor haben wir 98,3%, mit Api 20C AUX 90,8% und mit Mycotube 76,7% der Sproßpilzisolate korrekt bestimmt. Vor‐ und Nachteile dieser biochemi‐schen Differenzierungssysteme werden diskutiert. |
| doi_str_mv | 10.1111/j.1439-0507.1996.tb00116.x |
| format | Article |
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Zusammenfassung. Mit den klassischen Methoden der Fermentation und der Assimilation ist es aus arbeitstechnischen Gründen nicht möglich, in Routinelaboratorien die anfallenden Hefepilzisolate zu differenzieren. Aus diesen Gründen werden als Hilfsmittel kommerzielle biochemische Differenzierungssysteme eingesetzt. Drei dieser käuflich zu erwerbenden Bunten Reihen wurden mit 120 Sproßpilzisolaten auf ihre Aussagefähigkeit überprüft. Die morphologischen Merkmale dieser Pilzstämme waren für alle drei Differenzierungssysteme einheitlich. Mit Auxacolor haben wir 98,3%, mit Api 20C AUX 90,8% und mit Mycotube 76,7% der Sproßpilzisolate korrekt bestimmt. Vor‐ und Nachteile dieser biochemi‐schen Differenzierungssysteme werden diskutiert.</description><identifier>ISSN: 0933-7407</identifier><identifier>EISSN: 1439-0507</identifier><identifier>DOI: 10.1111/j.1439-0507.1996.tb00116.x</identifier><identifier>PMID: 8767008</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>API 20C AUX ; Auxacolor ; biochemical pattern ; Biological and medical sciences ; Candida - classification ; Candida - isolation & purification ; differentiation ; Fermentation ; Fundamental and applied biological sciences. Psychology ; Fungi - classification ; Fungi - isolation & purification ; Humans ; Microbiology ; morphological characteristics ; Mycological methods and techniques used in mycology ; Mycology ; Mycoses - microbiology ; Mycotube ; Reproducibility of Results ; Yeasts - classification ; Yeast‐like fungi</subject><ispartof>Mycoses, 1996-03, Vol.39 (3‐4), p.135-140</ispartof><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4305-e2ab102ea41e48ed7a92b14653d3594fcf0ebd222af3d13a535ff5039f74b4063</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1439-0507.1996.tb00116.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1439-0507.1996.tb00116.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3134477$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8767008$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hantschke, D.</creatorcontrib><title>Differentiation of yeast‐like fungi using the commercial Auxacolor system</title><title>Mycoses</title><addtitle>Mycoses</addtitle><description>Technical factors preclude the use of conventional fermentation and assimilation procedures for the routine laboratory differentiation of yeast‐like fungal isolates. Commercial biochemical differentiation systems are therefore used to remedy this situation. The predictive accuracy of three commercially available differentiation systems was tested using 120 yeast‐like fungal isolates. The morphological characteristics of these fungal strains were identical in the case of all three differentiation systems. The fungal isolates were correctly identified in 98.3% of cases with Auxacolor, in 90.8% of cases with API 20C AUX and in 76.7% of cases with the Mycotube system. The advantages and disadvantages of these biochemical differentiation systems are discussed.
Zusammenfassung. Mit den klassischen Methoden der Fermentation und der Assimilation ist es aus arbeitstechnischen Gründen nicht möglich, in Routinelaboratorien die anfallenden Hefepilzisolate zu differenzieren. Aus diesen Gründen werden als Hilfsmittel kommerzielle biochemische Differenzierungssysteme eingesetzt. Drei dieser käuflich zu erwerbenden Bunten Reihen wurden mit 120 Sproßpilzisolaten auf ihre Aussagefähigkeit überprüft. Die morphologischen Merkmale dieser Pilzstämme waren für alle drei Differenzierungssysteme einheitlich. Mit Auxacolor haben wir 98,3%, mit Api 20C AUX 90,8% und mit Mycotube 76,7% der Sproßpilzisolate korrekt bestimmt. Vor‐ und Nachteile dieser biochemi‐schen Differenzierungssysteme werden diskutiert.</description><subject>API 20C AUX</subject><subject>Auxacolor</subject><subject>biochemical pattern</subject><subject>Biological and medical sciences</subject><subject>Candida - classification</subject><subject>Candida - isolation & purification</subject><subject>differentiation</subject><subject>Fermentation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungi - classification</subject><subject>Fungi - isolation & purification</subject><subject>Humans</subject><subject>Microbiology</subject><subject>morphological characteristics</subject><subject>Mycological methods and techniques used in mycology</subject><subject>Mycology</subject><subject>Mycoses - microbiology</subject><subject>Mycotube</subject><subject>Reproducibility of Results</subject><subject>Yeasts - classification</subject><subject>Yeast‐like fungi</subject><issn>0933-7407</issn><issn>1439-0507</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1O20AQgFeIKg3QR0CyEOrN7uyfN3upFKVQEEFcyqGn1dqeTTf4J_XaIrnxCDxjn6SOYuXKXObwfZrDfIRcUUjoMN_WCRVcxyBBJVTrNOkyAErTZHtCpkd0SqagOY-VAPWZnIWwHiSlWTohk5lKFcBsSh5-eOewxbrztvNNHTUu2qEN3b-399K_YOT6euWjPvh6FXV_MMqbqsI297aM5v3W5k3ZtFHYhQ6rC_LJ2TLgl3Gfk-fbm1-Lu3j59PN-MV_GueAgY2Q2o8DQCopihoWymmVUpJIXXGrhcgeYFYwx63hBuZVcOieBa6dEJiDl5-Tr4e6mbf72GDpT-ZBjWdoamz4YNWMi1QAfilRqJkHLQbwcxT6rsDCb1le23ZnxTQO_HrkNuS1da-vch6PGKRdCqUH7ftBefYm7I6Zg9tnM2uzbmH0bs89mxmxmax5_LyiX_D_sJoy0</recordid><startdate>199603</startdate><enddate>199603</enddate><creator>Hantschke, D.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>199603</creationdate><title>Differentiation of yeast‐like fungi using the commercial Auxacolor system</title><author>Hantschke, D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4305-e2ab102ea41e48ed7a92b14653d3594fcf0ebd222af3d13a535ff5039f74b4063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>API 20C AUX</topic><topic>Auxacolor</topic><topic>biochemical pattern</topic><topic>Biological and medical sciences</topic><topic>Candida - classification</topic><topic>Candida - isolation & purification</topic><topic>differentiation</topic><topic>Fermentation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungi - classification</topic><topic>Fungi - isolation & purification</topic><topic>Humans</topic><topic>Microbiology</topic><topic>morphological characteristics</topic><topic>Mycological methods and techniques used in mycology</topic><topic>Mycology</topic><topic>Mycoses - microbiology</topic><topic>Mycotube</topic><topic>Reproducibility of Results</topic><topic>Yeasts - classification</topic><topic>Yeast‐like fungi</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hantschke, D.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Mycoses</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hantschke, D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differentiation of yeast‐like fungi using the commercial Auxacolor system</atitle><jtitle>Mycoses</jtitle><addtitle>Mycoses</addtitle><date>1996-03</date><risdate>1996</risdate><volume>39</volume><issue>3‐4</issue><spage>135</spage><epage>140</epage><pages>135-140</pages><issn>0933-7407</issn><eissn>1439-0507</eissn><abstract>Technical factors preclude the use of conventional fermentation and assimilation procedures for the routine laboratory differentiation of yeast‐like fungal isolates. Commercial biochemical differentiation systems are therefore used to remedy this situation. The predictive accuracy of three commercially available differentiation systems was tested using 120 yeast‐like fungal isolates. The morphological characteristics of these fungal strains were identical in the case of all three differentiation systems. The fungal isolates were correctly identified in 98.3% of cases with Auxacolor, in 90.8% of cases with API 20C AUX and in 76.7% of cases with the Mycotube system. The advantages and disadvantages of these biochemical differentiation systems are discussed.
Zusammenfassung. Mit den klassischen Methoden der Fermentation und der Assimilation ist es aus arbeitstechnischen Gründen nicht möglich, in Routinelaboratorien die anfallenden Hefepilzisolate zu differenzieren. Aus diesen Gründen werden als Hilfsmittel kommerzielle biochemische Differenzierungssysteme eingesetzt. Drei dieser käuflich zu erwerbenden Bunten Reihen wurden mit 120 Sproßpilzisolaten auf ihre Aussagefähigkeit überprüft. Die morphologischen Merkmale dieser Pilzstämme waren für alle drei Differenzierungssysteme einheitlich. Mit Auxacolor haben wir 98,3%, mit Api 20C AUX 90,8% und mit Mycotube 76,7% der Sproßpilzisolate korrekt bestimmt. Vor‐ und Nachteile dieser biochemi‐schen Differenzierungssysteme werden diskutiert.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>8767008</pmid><doi>10.1111/j.1439-0507.1996.tb00116.x</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | API 20C AUX Auxacolor biochemical pattern Biological and medical sciences Candida - classification Candida - isolation & purification differentiation Fermentation Fundamental and applied biological sciences. Psychology Fungi - classification Fungi - isolation & purification Humans Microbiology morphological characteristics Mycological methods and techniques used in mycology Mycology Mycoses - microbiology Mycotube Reproducibility of Results Yeasts - classification Yeast‐like fungi |
| title | Differentiation of yeast‐like fungi using the commercial Auxacolor system |
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