Histological evaluation of skin reconstruction using artificial dermis
An artificial dermis, composed of a collagen matrix, was applied to a full-thickness skin defect prepared on the back of rats. Two weeks later, a thin split-thickness skin autograft was overlaid on the matrix at each recipient site. The dermal layer at the recipient sites was 1.02 mm thick with prio...
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| Veröffentlicht in: | Biomaterials 1996, Vol.17 (10), p.995-1000 |
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| creator | Matsui, Risako Okura, Naganari Osaki, Ken-ichi Konishi, Jun Ikegami, kazuhito Koide, Mikio |
| description | An artificial dermis, composed of a collagen matrix, was applied to a full-thickness skin defect prepared on the back of rats. Two weeks later, a thin split-thickness skin autograft was overlaid on the matrix at each recipient site. The dermal layer at the recipient sites was 1.02 mm thick with prior application of artificial dermis, as compared with the 0.46 mm thickness observed without such pretreatment. Histologically, the split-thickness skin graft normally lies with no gap on the artificial dermis, which looks like natural dermis. Six days after grafting, the epithelial basal cells in the grafts showed an active uptake of bromodeoxyuridine (a thymidine analogue), indicating high activity of cell proliferation. About 50 and 20% respectively of the artificial dermis remained at each recipient site at 12 and 20 weeks after its application (after the skin defect). This finding indicates that bovine collagen, which is a constituent of the artificial dermis, is gradually replaced by the host tissue. |
| doi_str_mv | 10.1016/0142-9612(96)84674-6 |
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Two weeks later, a thin split-thickness skin autograft was overlaid on the matrix at each recipient site. The dermal layer at the recipient sites was 1.02 mm thick with prior application of artificial dermis, as compared with the 0.46 mm thickness observed without such pretreatment. Histologically, the split-thickness skin graft normally lies with no gap on the artificial dermis, which looks like natural dermis. Six days after grafting, the epithelial basal cells in the grafts showed an active uptake of bromodeoxyuridine (a thymidine analogue), indicating high activity of cell proliferation. About 50 and 20% respectively of the artificial dermis remained at each recipient site at 12 and 20 weeks after its application (after the skin defect). This finding indicates that bovine collagen, which is a constituent of the artificial dermis, is gradually replaced by the host tissue.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/0142-9612(96)84674-6</identifier><identifier>PMID: 8736734</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Animals ; Artificial dermis ; Biological and medical sciences ; Cattle ; collagen matrix ; Evaluation Studies as Topic ; heat-denatured atelocollagen ; Medical sciences ; Rats ; Rats, Sprague-Dawley ; Skin - cytology ; Skin Physiological Phenomena ; Skin plastic surgery ; Skin Transplantation - methods ; Skin, Artificial ; split-thickness skin graft ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Wounds and Injuries - surgery</subject><ispartof>Biomaterials, 1996, Vol.17 (10), p.995-1000</ispartof><rights>1996</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c483t-29d6f6638fea86bcf000db2a518f76f6d0c57cc25e471ccca3478cea96eccb193</citedby><cites>FETCH-LOGICAL-c483t-29d6f6638fea86bcf000db2a518f76f6d0c57cc25e471ccca3478cea96eccb193</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0142961296846746$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,4009,27902,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3119591$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8736734$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Matsui, Risako</creatorcontrib><creatorcontrib>Okura, Naganari</creatorcontrib><creatorcontrib>Osaki, Ken-ichi</creatorcontrib><creatorcontrib>Konishi, Jun</creatorcontrib><creatorcontrib>Ikegami, kazuhito</creatorcontrib><creatorcontrib>Koide, Mikio</creatorcontrib><title>Histological evaluation of skin reconstruction using artificial dermis</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>An artificial dermis, composed of a collagen matrix, was applied to a full-thickness skin defect prepared on the back of rats. Two weeks later, a thin split-thickness skin autograft was overlaid on the matrix at each recipient site. The dermal layer at the recipient sites was 1.02 mm thick with prior application of artificial dermis, as compared with the 0.46 mm thickness observed without such pretreatment. Histologically, the split-thickness skin graft normally lies with no gap on the artificial dermis, which looks like natural dermis. Six days after grafting, the epithelial basal cells in the grafts showed an active uptake of bromodeoxyuridine (a thymidine analogue), indicating high activity of cell proliferation. About 50 and 20% respectively of the artificial dermis remained at each recipient site at 12 and 20 weeks after its application (after the skin defect). This finding indicates that bovine collagen, which is a constituent of the artificial dermis, is gradually replaced by the host tissue.</description><subject>Animals</subject><subject>Artificial dermis</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>collagen matrix</subject><subject>Evaluation Studies as Topic</subject><subject>heat-denatured atelocollagen</subject><subject>Medical sciences</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Skin - cytology</subject><subject>Skin Physiological Phenomena</subject><subject>Skin plastic surgery</subject><subject>Skin Transplantation - methods</subject><subject>Skin, Artificial</subject><subject>split-thickness skin graft</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Wounds and Injuries - surgery</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1KJDEURoMo2vb4Bgq1kEEXNZNUfmsjiIy2IMxG1yF960ai1RVNqoR5-0nbTS91k5B857sJh5BTRn8xytRvykRTt4o1F626NEJpUas9MmNGm1q2VO6T2Q45Isc5v9BypqI5JIdGc6W5mJHbRchj7ONzANdX-OH6yY0hDlX0VX4NQ5UQ4pDHNMHn9ZTD8Fy5NAYfIJRKh2kV8g9y4F2f8WS7z8nT7Z_Hm0X98Pfu_ub6oQZh-Fg3bae8Utx4dEYtwVNKu2XjJDNel6SjIDVAI1FoBgCOC20AXasQYMlaPic_N3PfUnyfMI-2PA7Y927AOGWrTSMk5fpbkEnNG8ZkAcUGhBRzTujtWworl_5ZRu3as11LtGuJZbGfnq0qtbPt_Gm5wm5X2oot-fk2d7mY9ckNEPIO44y1smUFu9pgWKR9BEw2Q8ABsAtF_Gi7GL7-x39wLZpG</recordid><startdate>1996</startdate><enddate>1996</enddate><creator>Matsui, Risako</creator><creator>Okura, Naganari</creator><creator>Osaki, Ken-ichi</creator><creator>Konishi, Jun</creator><creator>Ikegami, kazuhito</creator><creator>Koide, Mikio</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>1996</creationdate><title>Histological evaluation of skin reconstruction using artificial dermis</title><author>Matsui, Risako ; Okura, Naganari ; Osaki, Ken-ichi ; Konishi, Jun ; Ikegami, kazuhito ; Koide, Mikio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c483t-29d6f6638fea86bcf000db2a518f76f6d0c57cc25e471ccca3478cea96eccb193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Artificial dermis</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>collagen matrix</topic><topic>Evaluation Studies as Topic</topic><topic>heat-denatured atelocollagen</topic><topic>Medical sciences</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Skin - cytology</topic><topic>Skin Physiological Phenomena</topic><topic>Skin plastic surgery</topic><topic>Skin Transplantation - methods</topic><topic>Skin, Artificial</topic><topic>split-thickness skin graft</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Wounds and Injuries - surgery</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Matsui, Risako</creatorcontrib><creatorcontrib>Okura, Naganari</creatorcontrib><creatorcontrib>Osaki, Ken-ichi</creatorcontrib><creatorcontrib>Konishi, Jun</creatorcontrib><creatorcontrib>Ikegami, kazuhito</creatorcontrib><creatorcontrib>Koide, Mikio</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Matsui, Risako</au><au>Okura, Naganari</au><au>Osaki, Ken-ichi</au><au>Konishi, Jun</au><au>Ikegami, kazuhito</au><au>Koide, Mikio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Histological evaluation of skin reconstruction using artificial dermis</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>1996</date><risdate>1996</risdate><volume>17</volume><issue>10</issue><spage>995</spage><epage>1000</epage><pages>995-1000</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>An artificial dermis, composed of a collagen matrix, was applied to a full-thickness skin defect prepared on the back of rats. Two weeks later, a thin split-thickness skin autograft was overlaid on the matrix at each recipient site. The dermal layer at the recipient sites was 1.02 mm thick with prior application of artificial dermis, as compared with the 0.46 mm thickness observed without such pretreatment. Histologically, the split-thickness skin graft normally lies with no gap on the artificial dermis, which looks like natural dermis. Six days after grafting, the epithelial basal cells in the grafts showed an active uptake of bromodeoxyuridine (a thymidine analogue), indicating high activity of cell proliferation. About 50 and 20% respectively of the artificial dermis remained at each recipient site at 12 and 20 weeks after its application (after the skin defect). This finding indicates that bovine collagen, which is a constituent of the artificial dermis, is gradually replaced by the host tissue.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>8736734</pmid><doi>10.1016/0142-9612(96)84674-6</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biomaterials, 1996, Vol.17 (10), p.995-1000 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Artificial dermis Biological and medical sciences Cattle collagen matrix Evaluation Studies as Topic heat-denatured atelocollagen Medical sciences Rats Rats, Sprague-Dawley Skin - cytology Skin Physiological Phenomena Skin plastic surgery Skin Transplantation - methods Skin, Artificial split-thickness skin graft Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Wounds and Injuries - surgery |
| title | Histological evaluation of skin reconstruction using artificial dermis |
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