Species Specificity of Iron Delivery in Hybridomas
Studies with Human x Human (HxH), Human x Mouse (HxM), and Mouse x Mouse (MxM) hybridomas have enabled us to define specific factors that affect hybridoma growth in a species-specific manner. Three transferons and three lipophilic iron chelates have been tested for their ability to support hybridoma...
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| Veröffentlicht in: | In Vitro Cellular & Developmental Biology 1988-05, Vol.24 (5), p.413-419 |
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| container_title | In Vitro Cellular & Developmental Biology |
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| creator | Ill, Charles R. Tammy Brehm Bjorn K. Lydersen Rachel Hernandez Burnett, Karen G. |
| description | Studies with Human x Human (HxH), Human x Mouse (HxM), and Mouse x Mouse (MxM) hybridomas have enabled us to define specific factors that affect hybridoma growth in a species-specific manner. Three transferons and three lipophilic iron chelates have been tested for their ability to support hybridoma proliferation and antibody production. The results of these studies demonstrate that HxH hybridomas do not respond to bovine transferrin$a^\dag$concentrations up to$100 \mu g/ml$and are approximately 100-fold less responsive to mouse transferrin than to human transferrin. HxM and MxM hybridomas respond equally to human or mouse transferrin but are 100-fold less sensitive to bovine transferrin. An antibody to the human transferrin receptor inhibited the growth-promoting activity of human or mouse transferrin on HxH hybridomas but was ineffective on HxM hybridomas. This demonstrated the functionality of the human transferrin receptor in HxH hybridomas and that human, mouse, and bovine transferrin were interacting through the mouse transferrin receptor in HxM hybridomas. HxH and HxM hybridomas respond similarly to three different iron chelates exhibiting 80 to 110% of the growth response to human transferrin. MxM hybridomas fail to respond to the iron chelates at similar concentrations, suggesting that the human genome present in the other hybridoma species confers a unique ability for utilizing iron when delivered in this form. |
| doi_str_mv | 10.1007/BF02628492 |
| format | Article |
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Lydersen ; Rachel Hernandez ; Burnett, Karen G.</creator><creatorcontrib>Ill, Charles R. ; Tammy Brehm ; Bjorn K. Lydersen ; Rachel Hernandez ; Burnett, Karen G.</creatorcontrib><description>Studies with Human x Human (HxH), Human x Mouse (HxM), and Mouse x Mouse (MxM) hybridomas have enabled us to define specific factors that affect hybridoma growth in a species-specific manner. Three transferons and three lipophilic iron chelates have been tested for their ability to support hybridoma proliferation and antibody production. The results of these studies demonstrate that HxH hybridomas do not respond to bovine transferrin$a^\dag$concentrations up to$100 \mu g/ml$and are approximately 100-fold less responsive to mouse transferrin than to human transferrin. HxM and MxM hybridomas respond equally to human or mouse transferrin but are 100-fold less sensitive to bovine transferrin. An antibody to the human transferrin receptor inhibited the growth-promoting activity of human or mouse transferrin on HxH hybridomas but was ineffective on HxM hybridomas. This demonstrated the functionality of the human transferrin receptor in HxH hybridomas and that human, mouse, and bovine transferrin were interacting through the mouse transferrin receptor in HxM hybridomas. HxH and HxM hybridomas respond similarly to three different iron chelates exhibiting 80 to 110% of the growth response to human transferrin. MxM hybridomas fail to respond to the iron chelates at similar concentrations, suggesting that the human genome present in the other hybridoma species confers a unique ability for utilizing iron when delivered in this form.</description><identifier>ISSN: 0883-8364</identifier><identifier>ISSN: 0073-5655</identifier><identifier>EISSN: 2327-431X</identifier><identifier>EISSN: 1475-2689</identifier><identifier>DOI: 10.1007/BF02628492</identifier><identifier>PMID: 3372446</identifier><identifier>CODEN: ICDBEO</identifier><language>eng</language><publisher>Largo, MD: Tissue Culture Association, Inc</publisher><subject>Animal cells ; Animals ; Antibodies ; Biological and medical sciences ; Biotechnology ; Cattle ; Cell cultures. Hybridization. Fusion ; Cell growth ; Cell hybridization, cell fusion, hybridomas ; Cell Line ; Cell lines ; Chelates ; chelating agents ; Citrates ; Electrophoresis, Polyacrylamide Gel ; Enzyme-Linked Immunosorbent Assay ; Eukaryotic cell cultures ; Fundamental and applied biological sciences. Psychology ; Humans ; hybridoma ; Hybridomas ; Hybridomas - metabolism ; iron ; Iron - metabolism ; Methods. Procedures. Technologies ; Mice ; Molecular and cellular biology ; Receptors, Transferrin - metabolism ; Species Specificity ; transferrin ; Transferrin - pharmacology ; Transferrin receptors ; Transferrins ; Ungulates</subject><ispartof>In Vitro Cellular & Developmental Biology, 1988-05, Vol.24 (5), p.413-419</ispartof><rights>Copyright 1988 Tissue Culture Association</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c363t-ab15daca57868c702930b051fe96ba5db0d2ee9196d0d8ab7a41a5efe55ef6a43</citedby><cites>FETCH-LOGICAL-c363t-ab15daca57868c702930b051fe96ba5db0d2ee9196d0d8ab7a41a5efe55ef6a43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4296236$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4296236$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27923,27924,58016,58249</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7071516$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3372446$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ill, Charles R.</creatorcontrib><creatorcontrib>Tammy Brehm</creatorcontrib><creatorcontrib>Bjorn K. Lydersen</creatorcontrib><creatorcontrib>Rachel Hernandez</creatorcontrib><creatorcontrib>Burnett, Karen G.</creatorcontrib><title>Species Specificity of Iron Delivery in Hybridomas</title><title>In Vitro Cellular & Developmental Biology</title><addtitle>In Vitro Cell Dev Biol</addtitle><description>Studies with Human x Human (HxH), Human x Mouse (HxM), and Mouse x Mouse (MxM) hybridomas have enabled us to define specific factors that affect hybridoma growth in a species-specific manner. Three transferons and three lipophilic iron chelates have been tested for their ability to support hybridoma proliferation and antibody production. The results of these studies demonstrate that HxH hybridomas do not respond to bovine transferrin$a^\dag$concentrations up to$100 \mu g/ml$and are approximately 100-fold less responsive to mouse transferrin than to human transferrin. HxM and MxM hybridomas respond equally to human or mouse transferrin but are 100-fold less sensitive to bovine transferrin. An antibody to the human transferrin receptor inhibited the growth-promoting activity of human or mouse transferrin on HxH hybridomas but was ineffective on HxM hybridomas. This demonstrated the functionality of the human transferrin receptor in HxH hybridomas and that human, mouse, and bovine transferrin were interacting through the mouse transferrin receptor in HxM hybridomas. HxH and HxM hybridomas respond similarly to three different iron chelates exhibiting 80 to 110% of the growth response to human transferrin. MxM hybridomas fail to respond to the iron chelates at similar concentrations, suggesting that the human genome present in the other hybridoma species confers a unique ability for utilizing iron when delivered in this form.</description><subject>Animal cells</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cattle</subject><subject>Cell cultures. Hybridization. Fusion</subject><subject>Cell growth</subject><subject>Cell hybridization, cell fusion, hybridomas</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>Chelates</subject><subject>chelating agents</subject><subject>Citrates</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Eukaryotic cell cultures</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>hybridoma</subject><subject>Hybridomas</subject><subject>Hybridomas - metabolism</subject><subject>iron</subject><subject>Iron - metabolism</subject><subject>Methods. Procedures. Technologies</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Receptors, Transferrin - metabolism</subject><subject>Species Specificity</subject><subject>transferrin</subject><subject>Transferrin - pharmacology</subject><subject>Transferrin receptors</subject><subject>Transferrins</subject><subject>Ungulates</subject><issn>0883-8364</issn><issn>0073-5655</issn><issn>2327-431X</issn><issn>1475-2689</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0L1Lw0AYBvBDlFqri7NCBnEQou99X8ZarS0UHFRwC5fLBa7ko96lQv57Uxvq6PK-w_PjGR6ELjHcYwD58DgHIohiCTlCY0KJjBnFn8doDErRWFHBTtFZCGsACoKQERpRKgljYozI28YaZ0P0-wtnXNtFTREtfVNHT7Z039Z3kaujRZd5lzeVDufopNBlsBfDn6CP-fP7bBGvXl-Ws-kqNlTQNtYZ5rk2mksllJFAEgoZcFzYRGSa5xnkxNoEJyKHXOlMaoY1t4Xl_RGa0Qm63fdufPO1taFNKxeMLUtd22YbUqkIwyCSfyHmAIwI2sO7PTS-CcHbIt14V2nfpRjS3ZLp35I9vh5at1ll8wMdpuvzmyHXweiy8Lo2LhyYBIk53rGrPVuHtvGHmJFEkH6nH2o2gow</recordid><startdate>19880501</startdate><enddate>19880501</enddate><creator>Ill, Charles R.</creator><creator>Tammy Brehm</creator><creator>Bjorn K. Lydersen</creator><creator>Rachel Hernandez</creator><creator>Burnett, Karen G.</creator><general>Tissue Culture Association, Inc</general><general>Society for In Vitro Biology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19880501</creationdate><title>Species Specificity of Iron Delivery in Hybridomas</title><author>Ill, Charles R. ; Tammy Brehm ; Bjorn K. Lydersen ; Rachel Hernandez ; Burnett, Karen G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c363t-ab15daca57868c702930b051fe96ba5db0d2ee9196d0d8ab7a41a5efe55ef6a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Animal cells</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cattle</topic><topic>Cell cultures. Hybridization. Fusion</topic><topic>Cell growth</topic><topic>Cell hybridization, cell fusion, hybridomas</topic><topic>Cell Line</topic><topic>Cell lines</topic><topic>Chelates</topic><topic>chelating agents</topic><topic>Citrates</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Eukaryotic cell cultures</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>hybridoma</topic><topic>Hybridomas</topic><topic>Hybridomas - metabolism</topic><topic>iron</topic><topic>Iron - metabolism</topic><topic>Methods. Procedures. Technologies</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Receptors, Transferrin - metabolism</topic><topic>Species Specificity</topic><topic>transferrin</topic><topic>Transferrin - pharmacology</topic><topic>Transferrin receptors</topic><topic>Transferrins</topic><topic>Ungulates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ill, Charles R.</creatorcontrib><creatorcontrib>Tammy Brehm</creatorcontrib><creatorcontrib>Bjorn K. Lydersen</creatorcontrib><creatorcontrib>Rachel Hernandez</creatorcontrib><creatorcontrib>Burnett, Karen G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>In Vitro Cellular & Developmental Biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ill, Charles R.</au><au>Tammy Brehm</au><au>Bjorn K. Lydersen</au><au>Rachel Hernandez</au><au>Burnett, Karen G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Species Specificity of Iron Delivery in Hybridomas</atitle><jtitle>In Vitro Cellular & Developmental Biology</jtitle><addtitle>In Vitro Cell Dev Biol</addtitle><date>1988-05-01</date><risdate>1988</risdate><volume>24</volume><issue>5</issue><spage>413</spage><epage>419</epage><pages>413-419</pages><issn>0883-8364</issn><issn>0073-5655</issn><eissn>2327-431X</eissn><eissn>1475-2689</eissn><coden>ICDBEO</coden><abstract>Studies with Human x Human (HxH), Human x Mouse (HxM), and Mouse x Mouse (MxM) hybridomas have enabled us to define specific factors that affect hybridoma growth in a species-specific manner. Three transferons and three lipophilic iron chelates have been tested for their ability to support hybridoma proliferation and antibody production. The results of these studies demonstrate that HxH hybridomas do not respond to bovine transferrin$a^\dag$concentrations up to$100 \mu g/ml$and are approximately 100-fold less responsive to mouse transferrin than to human transferrin. HxM and MxM hybridomas respond equally to human or mouse transferrin but are 100-fold less sensitive to bovine transferrin. An antibody to the human transferrin receptor inhibited the growth-promoting activity of human or mouse transferrin on HxH hybridomas but was ineffective on HxM hybridomas. This demonstrated the functionality of the human transferrin receptor in HxH hybridomas and that human, mouse, and bovine transferrin were interacting through the mouse transferrin receptor in HxM hybridomas. HxH and HxM hybridomas respond similarly to three different iron chelates exhibiting 80 to 110% of the growth response to human transferrin. MxM hybridomas fail to respond to the iron chelates at similar concentrations, suggesting that the human genome present in the other hybridoma species confers a unique ability for utilizing iron when delivered in this form.</abstract><cop>Largo, MD</cop><pub>Tissue Culture Association, Inc</pub><pmid>3372446</pmid><doi>10.1007/BF02628492</doi><tpages>7</tpages></addata></record> |
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| ispartof | In Vitro Cellular & Developmental Biology, 1988-05, Vol.24 (5), p.413-419 |
| issn | 0883-8364 0073-5655 2327-431X 1475-2689 |
| language | eng |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; SpringerLink Journals - AutoHoldings |
| subjects | Animal cells Animals Antibodies Biological and medical sciences Biotechnology Cattle Cell cultures. Hybridization. Fusion Cell growth Cell hybridization, cell fusion, hybridomas Cell Line Cell lines Chelates chelating agents Citrates Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Humans hybridoma Hybridomas Hybridomas - metabolism iron Iron - metabolism Methods. Procedures. Technologies Mice Molecular and cellular biology Receptors, Transferrin - metabolism Species Specificity transferrin Transferrin - pharmacology Transferrin receptors Transferrins Ungulates |
| title | Species Specificity of Iron Delivery in Hybridomas |
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