Vaccination of cattle with bovine papillomavirus type 4 L2 elicits the production of virus-neutralizing antibodies
1 Department of Veterinary Pathology and 2 the Beatson Institute for Cancer Research, Garscube Estate, Glasgow G61 1BD, UK Prophylactic vaccination of cattle with the N terminus (L2a, aa 11200) of the minor capsid protein L2 completely prevented bovine papillomavirus type 4 (BPV-4) infection of the...
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| Veröffentlicht in: | Journal of general virology 1996-07, Vol.77 (7), p.1577-1583 |
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| container_title | Journal of general virology |
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| creator | Gaukroger, J. M Chandrachud, L. M O'Neil, B. W Grindlay, G. J Knowles, G Campo, M. S |
| description | 1 Department of Veterinary Pathology
and 2 the Beatson Institute for Cancer Research, Garscube Estate, Glasgow G61 1BD, UK
Prophylactic vaccination of cattle with the N terminus (L2a, aa 11200) of the minor capsid protein L2 completely prevented bovine papillomavirus type 4 (BPV-4) infection of the alimentary canal. To investigate the mechanisms underlying protection from viral infection, sera from vaccinated animals were analysed in neutralization assays both in the nude mouse xenograft system and in cattle. BPV-4 retained its infectivity when incubated with pre-immune cattle sera, whereas, when incubated with immune sera from animals vaccinated with either whole L2 or its N terminus L2a, its infectivity was greatly reduced, indicating that the immune sera had neutralizing activity against the virus. This activity could be abrogated by absorbing the immune sera with L2 or L2a, thus indicating that virus neutralization was due to the presence in the immune sera of anti-L2 antibodies.
Received 25 January 1996;
accepted 1 March 1996. |
| doi_str_mv | 10.1099/0022-1317-77-7-1577 |
| format | Article |
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and 2 the Beatson Institute for Cancer Research, Garscube Estate, Glasgow G61 1BD, UK
Prophylactic vaccination of cattle with the N terminus (L2a, aa 11200) of the minor capsid protein L2 completely prevented bovine papillomavirus type 4 (BPV-4) infection of the alimentary canal. To investigate the mechanisms underlying protection from viral infection, sera from vaccinated animals were analysed in neutralization assays both in the nude mouse xenograft system and in cattle. BPV-4 retained its infectivity when incubated with pre-immune cattle sera, whereas, when incubated with immune sera from animals vaccinated with either whole L2 or its N terminus L2a, its infectivity was greatly reduced, indicating that the immune sera had neutralizing activity against the virus. This activity could be abrogated by absorbing the immune sera with L2 or L2a, thus indicating that virus neutralization was due to the presence in the immune sera of anti-L2 antibodies.
Received 25 January 1996;
accepted 1 March 1996.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-77-7-1577</identifier><identifier>PMID: 8758002</identifier><language>eng</language><publisher>England: Soc General Microbiol</publisher><subject>Animals ; Antibodies, Viral - immunology ; bovine papillomavirus ; Bovine papillomavirus 1 - immunology ; Bovine papillomavirus 4 ; Capsid - administration & dosage ; Capsid - immunology ; Capsid Proteins ; Cattle ; Female ; Mice ; Mice, Nude ; Neutralization Tests ; Papillomavirus Infections - immunology ; Papillomavirus Infections - prevention & control ; Recombinant Fusion Proteins - immunology ; Tumor Virus Infections - immunology ; Tumor Virus Infections - prevention & control ; Vaccination ; Vaccines, Synthetic - administration & dosage ; Vaccines, Synthetic - immunology ; Viral Vaccines - administration & dosage ; Viral Vaccines - immunology ; Virus Replication</subject><ispartof>Journal of general virology, 1996-07, Vol.77 (7), p.1577-1583</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-de6a23a2a07ca4676a809075c202ba15ffb755c1c88038b3bbc3d589f1db9abf3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3746,3747,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8758002$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gaukroger, J. M</creatorcontrib><creatorcontrib>Chandrachud, L. M</creatorcontrib><creatorcontrib>O'Neil, B. W</creatorcontrib><creatorcontrib>Grindlay, G. J</creatorcontrib><creatorcontrib>Knowles, G</creatorcontrib><creatorcontrib>Campo, M. S</creatorcontrib><title>Vaccination of cattle with bovine papillomavirus type 4 L2 elicits the production of virus-neutralizing antibodies</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>1 Department of Veterinary Pathology
and 2 the Beatson Institute for Cancer Research, Garscube Estate, Glasgow G61 1BD, UK
Prophylactic vaccination of cattle with the N terminus (L2a, aa 11200) of the minor capsid protein L2 completely prevented bovine papillomavirus type 4 (BPV-4) infection of the alimentary canal. To investigate the mechanisms underlying protection from viral infection, sera from vaccinated animals were analysed in neutralization assays both in the nude mouse xenograft system and in cattle. BPV-4 retained its infectivity when incubated with pre-immune cattle sera, whereas, when incubated with immune sera from animals vaccinated with either whole L2 or its N terminus L2a, its infectivity was greatly reduced, indicating that the immune sera had neutralizing activity against the virus. This activity could be abrogated by absorbing the immune sera with L2 or L2a, thus indicating that virus neutralization was due to the presence in the immune sera of anti-L2 antibodies.
Received 25 January 1996;
accepted 1 March 1996.</description><subject>Animals</subject><subject>Antibodies, Viral - immunology</subject><subject>bovine papillomavirus</subject><subject>Bovine papillomavirus 1 - immunology</subject><subject>Bovine papillomavirus 4</subject><subject>Capsid - administration & dosage</subject><subject>Capsid - immunology</subject><subject>Capsid Proteins</subject><subject>Cattle</subject><subject>Female</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Neutralization Tests</subject><subject>Papillomavirus Infections - immunology</subject><subject>Papillomavirus Infections - prevention & control</subject><subject>Recombinant Fusion Proteins - immunology</subject><subject>Tumor Virus Infections - immunology</subject><subject>Tumor Virus Infections - prevention & control</subject><subject>Vaccination</subject><subject>Vaccines, Synthetic - administration & dosage</subject><subject>Vaccines, Synthetic - immunology</subject><subject>Viral Vaccines - administration & dosage</subject><subject>Viral Vaccines - immunology</subject><subject>Virus Replication</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFr3DAQhUVpSTZpf0Ep6BR6cSrJliUfQ0ibwEIvba9iJMu7U2xrI8kJya-vtrtNjoUBgd43T-I9Qj5ydslZ131hTIiK11xVqkzFpVJvyIo3raxE0d-S1QtxSs5S-s0YbxqpTsiJVlIXcUXiL3AOZ8gYZhoG6iDn0dNHzFtqwwPOnu5gh-MYJnjAuCSan3aeNnQtqB_RYS432wLF0C_un8tfspr9kiOM-IzzhsKc0YYefXpP3g0wJv_heJ6Tn19vflzfVuvv3-6ur9aVa1iXq963IGoQwJSDplUtaNYxJZ1gwgKXw2CVlI47rVmtbW2tq3upu4H3tgM71Ofk4uBb_na_-JTNhMn5cYTZhyUZpUXdcN38F-SyFZJrXsD6ALoYUop-MLuIE8Qnw5nZV2L2gZt94EaVMftKytano_1iJ9-_7Bw7KPrng77FzfYRozcbP09Y3rAYTIny1eoPOi-XJw</recordid><startdate>19960701</startdate><enddate>19960701</enddate><creator>Gaukroger, J. 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S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-de6a23a2a07ca4676a809075c202ba15ffb755c1c88038b3bbc3d589f1db9abf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Antibodies, Viral - immunology</topic><topic>bovine papillomavirus</topic><topic>Bovine papillomavirus 1 - immunology</topic><topic>Bovine papillomavirus 4</topic><topic>Capsid - administration & dosage</topic><topic>Capsid - immunology</topic><topic>Capsid Proteins</topic><topic>Cattle</topic><topic>Female</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>Neutralization Tests</topic><topic>Papillomavirus Infections - immunology</topic><topic>Papillomavirus Infections - prevention & control</topic><topic>Recombinant Fusion Proteins - immunology</topic><topic>Tumor Virus Infections - immunology</topic><topic>Tumor Virus Infections - prevention & control</topic><topic>Vaccination</topic><topic>Vaccines, Synthetic - administration & dosage</topic><topic>Vaccines, Synthetic - immunology</topic><topic>Viral Vaccines - administration & dosage</topic><topic>Viral Vaccines - immunology</topic><topic>Virus Replication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gaukroger, J. M</creatorcontrib><creatorcontrib>Chandrachud, L. M</creatorcontrib><creatorcontrib>O'Neil, B. W</creatorcontrib><creatorcontrib>Grindlay, G. J</creatorcontrib><creatorcontrib>Knowles, G</creatorcontrib><creatorcontrib>Campo, M. S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gaukroger, J. M</au><au>Chandrachud, L. M</au><au>O'Neil, B. W</au><au>Grindlay, G. J</au><au>Knowles, G</au><au>Campo, M. S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Vaccination of cattle with bovine papillomavirus type 4 L2 elicits the production of virus-neutralizing antibodies</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1996-07-01</date><risdate>1996</risdate><volume>77</volume><issue>7</issue><spage>1577</spage><epage>1583</epage><pages>1577-1583</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><abstract>1 Department of Veterinary Pathology
and 2 the Beatson Institute for Cancer Research, Garscube Estate, Glasgow G61 1BD, UK
Prophylactic vaccination of cattle with the N terminus (L2a, aa 11200) of the minor capsid protein L2 completely prevented bovine papillomavirus type 4 (BPV-4) infection of the alimentary canal. To investigate the mechanisms underlying protection from viral infection, sera from vaccinated animals were analysed in neutralization assays both in the nude mouse xenograft system and in cattle. BPV-4 retained its infectivity when incubated with pre-immune cattle sera, whereas, when incubated with immune sera from animals vaccinated with either whole L2 or its N terminus L2a, its infectivity was greatly reduced, indicating that the immune sera had neutralizing activity against the virus. This activity could be abrogated by absorbing the immune sera with L2 or L2a, thus indicating that virus neutralization was due to the presence in the immune sera of anti-L2 antibodies.
Received 25 January 1996;
accepted 1 March 1996.</abstract><cop>England</cop><pub>Soc General Microbiol</pub><pmid>8758002</pmid><doi>10.1099/0022-1317-77-7-1577</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Antibodies, Viral - immunology bovine papillomavirus Bovine papillomavirus 1 - immunology Bovine papillomavirus 4 Capsid - administration & dosage Capsid - immunology Capsid Proteins Cattle Female Mice Mice, Nude Neutralization Tests Papillomavirus Infections - immunology Papillomavirus Infections - prevention & control Recombinant Fusion Proteins - immunology Tumor Virus Infections - immunology Tumor Virus Infections - prevention & control Vaccination Vaccines, Synthetic - administration & dosage Vaccines, Synthetic - immunology Viral Vaccines - administration & dosage Viral Vaccines - immunology Virus Replication |
| title | Vaccination of cattle with bovine papillomavirus type 4 L2 elicits the production of virus-neutralizing antibodies |
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