Organotypic cortex-striatum-mesencephalon cultures: the nigrostriatal pathway

We were successful in developing cortex-striatum-mesencephalon organotypic cultures from the rat brain after 4–9 weeks in vitro. A modification of the ‘roller tube’ technique was employed where slices were embedded in a plasma/thrombin clot onto a Millicell membrane on a coverslip. The underlying me...

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