Giardia duodenalis: Exposure to Metronidazole Inhibits Competitive Interactions between Isolates of the Parasite In vitro
The competitive interactions of genetically distinct isolates of Giardia duodenalis with different growth rates were studied in vitro. Electrophoretic analysis of mixed cultures showed that competition between 2 cloned isolates occurs under normal in vitro culture conditions, with faster-growing iso...
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| Veröffentlicht in: | The Journal of parasitology 1996-08, Vol.82 (4), p.679-683 |
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| container_end_page | 683 |
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| container_issue | 4 |
| container_start_page | 679 |
| container_title | The Journal of parasitology |
| container_volume | 82 |
| creator | Thompson, R. C. A. Lymbery, A. J. Pearce, D. A. Finn, K. C. Reynoldson, J. A. Meloni, B. P. |
| description | The competitive interactions of genetically distinct isolates of Giardia duodenalis with different growth rates were studied in vitro. Electrophoretic analysis of mixed cultures showed that competition between 2 cloned isolates occurs under normal in vitro culture conditions, with faster-growing isolates outcompeting those with slower growth rates. The addition of sublethal concentrations of metronidazole to clonal mixtures in vitro prevented the competitive exclusion, which was seen in normal culture. This apparently occurred because the drug reduced the growth rate of the faster-growing but not the slower-growing clone. |
| doi_str_mv | 10.2307/3283808 |
| format | Article |
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C. A. ; Lymbery, A. J. ; Pearce, D. A. ; Finn, K. C. ; Reynoldson, J. A. ; Meloni, B. P.</creator><creatorcontrib>Thompson, R. C. A. ; Lymbery, A. J. ; Pearce, D. A. ; Finn, K. C. ; Reynoldson, J. A. ; Meloni, B. P.</creatorcontrib><description>The competitive interactions of genetically distinct isolates of Giardia duodenalis with different growth rates were studied in vitro. Electrophoretic analysis of mixed cultures showed that competition between 2 cloned isolates occurs under normal in vitro culture conditions, with faster-growing isolates outcompeting those with slower growth rates. The addition of sublethal concentrations of metronidazole to clonal mixtures in vitro prevented the competitive exclusion, which was seen in normal culture. This apparently occurred because the drug reduced the growth rate of the faster-growing but not the slower-growing clone.</description><identifier>ISSN: 0022-3395</identifier><identifier>EISSN: 1937-2345</identifier><identifier>DOI: 10.2307/3283808</identifier><identifier>PMID: 8691391</identifier><identifier>CODEN: JOPAA2</identifier><language>eng</language><publisher>Lawrence, KS: American Society of Parasitologists</publisher><subject>Animals ; Antibiotics. Antiinfectious agents. 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Drug treatments ; Phosphoglucomutase - analysis ; Phosphoglucomutase - genetics ; Population genetics ; Research Notes ; Trophozoites</subject><ispartof>The Journal of parasitology, 1996-08, Vol.82 (4), p.679-683</ispartof><rights>Copyright 1996 American Society of Parasitologists</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c398t-7950d635da14105bc38520bae69bb6aae032aa5d155e69b376d0b554f251e9933</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3283808$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3283808$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3181258$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8691391$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thompson, R. C. A.</creatorcontrib><creatorcontrib>Lymbery, A. J.</creatorcontrib><creatorcontrib>Pearce, D. A.</creatorcontrib><creatorcontrib>Finn, K. C.</creatorcontrib><creatorcontrib>Reynoldson, J. A.</creatorcontrib><creatorcontrib>Meloni, B. P.</creatorcontrib><title>Giardia duodenalis: Exposure to Metronidazole Inhibits Competitive Interactions between Isolates of the Parasite In vitro</title><title>The Journal of parasitology</title><addtitle>J Parasitol</addtitle><description>The competitive interactions of genetically distinct isolates of Giardia duodenalis with different growth rates were studied in vitro. Electrophoretic analysis of mixed cultures showed that competition between 2 cloned isolates occurs under normal in vitro culture conditions, with faster-growing isolates outcompeting those with slower growth rates. The addition of sublethal concentrations of metronidazole to clonal mixtures in vitro prevented the competitive exclusion, which was seen in normal culture. This apparently occurred because the drug reduced the growth rate of the faster-growing but not the slower-growing clone.</description><subject>Animals</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Antiparasitic agents</subject><subject>Antiprotozoal Agents - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Electrophoresis, Starch Gel</subject><subject>Genetic diversity</subject><subject>Genetic heterogeneity</subject><subject>Genetic Variation</subject><subject>Giardia - drug effects</subject><subject>Giardia - enzymology</subject><subject>Giardia - genetics</subject><subject>Giardia - growth & development</subject><subject>Giardia duodenalis</subject><subject>Hexokinase - analysis</subject><subject>Hexokinase - genetics</subject><subject>Human genetics</subject><subject>Humans</subject><subject>Ice</subject><subject>Medical genetics</subject><subject>Medical sciences</subject><subject>Metronidazole - pharmacology</subject><subject>Parasites</subject><subject>Parasitology</subject><subject>Pharmacology. Drug treatments</subject><subject>Phosphoglucomutase - analysis</subject><subject>Phosphoglucomutase - genetics</subject><subject>Population genetics</subject><subject>Research Notes</subject><subject>Trophozoites</subject><issn>0022-3395</issn><issn>1937-2345</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9LHEEQxZsQMRsNfgKhDyGexvSf7Zlub7KoWVDiQc9DzXQNtsxOr109JubTO4uDOYUcioL3fryCV4wdSXGqtKi-a2W1FfYDW0inq0LppfnIFkIoVWjtzCf2mehRCGGm2Wf7tnRSO7lgL1cBkg_A_Rg9DtAHOuMXv7eRxoQ8R36DOcUhePgTe-Tr4SE0IRNfxc0Wc8jheSdmTNDmEAfiDeZfiANfU-whI_HY8fyA_BYSUMg7mj-HKfOQ7XXQE36Z9wG7v7y4W_0orn9erVfn10Wrnc1F5YzwpTYe5FIK07TaGiUawNI1TQmAQisA46UxO0lXpReNMctOGYnOaX3Avr3lblN8GpFyvQnUYt_DgHGkurJKTV3Y_4LSlE6rpZvAkzewTZEoYVdvU9hAeqmlqHffqOdvTOTxHDk2G_Tv3Fz_5H-dfaAW-i7B0AZ6x7S0Uhn7F3ukHNM_r70Cbuyc4g</recordid><startdate>19960801</startdate><enddate>19960801</enddate><creator>Thompson, R. C. 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P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-7950d635da14105bc38520bae69bb6aae032aa5d155e69b376d0b554f251e9933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>Antiparasitic agents</topic><topic>Antiprotozoal Agents - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Electrophoresis, Starch Gel</topic><topic>Genetic diversity</topic><topic>Genetic heterogeneity</topic><topic>Genetic Variation</topic><topic>Giardia - drug effects</topic><topic>Giardia - enzymology</topic><topic>Giardia - genetics</topic><topic>Giardia - growth & development</topic><topic>Giardia duodenalis</topic><topic>Hexokinase - analysis</topic><topic>Hexokinase - genetics</topic><topic>Human genetics</topic><topic>Humans</topic><topic>Ice</topic><topic>Medical genetics</topic><topic>Medical sciences</topic><topic>Metronidazole - pharmacology</topic><topic>Parasites</topic><topic>Parasitology</topic><topic>Pharmacology. Drug treatments</topic><topic>Phosphoglucomutase - analysis</topic><topic>Phosphoglucomutase - genetics</topic><topic>Population genetics</topic><topic>Research Notes</topic><topic>Trophozoites</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thompson, R. C. A.</creatorcontrib><creatorcontrib>Lymbery, A. J.</creatorcontrib><creatorcontrib>Pearce, D. A.</creatorcontrib><creatorcontrib>Finn, K. C.</creatorcontrib><creatorcontrib>Reynoldson, J. A.</creatorcontrib><creatorcontrib>Meloni, B. 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P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Giardia duodenalis: Exposure to Metronidazole Inhibits Competitive Interactions between Isolates of the Parasite In vitro</atitle><jtitle>The Journal of parasitology</jtitle><addtitle>J Parasitol</addtitle><date>1996-08-01</date><risdate>1996</risdate><volume>82</volume><issue>4</issue><spage>679</spage><epage>683</epage><pages>679-683</pages><issn>0022-3395</issn><eissn>1937-2345</eissn><coden>JOPAA2</coden><abstract>The competitive interactions of genetically distinct isolates of Giardia duodenalis with different growth rates were studied in vitro. Electrophoretic analysis of mixed cultures showed that competition between 2 cloned isolates occurs under normal in vitro culture conditions, with faster-growing isolates outcompeting those with slower growth rates. The addition of sublethal concentrations of metronidazole to clonal mixtures in vitro prevented the competitive exclusion, which was seen in normal culture. This apparently occurred because the drug reduced the growth rate of the faster-growing but not the slower-growing clone.</abstract><cop>Lawrence, KS</cop><pub>American Society of Parasitologists</pub><pmid>8691391</pmid><doi>10.2307/3283808</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of parasitology, 1996-08, Vol.82 (4), p.679-683 |
| issn | 0022-3395 1937-2345 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78221398 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing |
| subjects | Animals Antibiotics. Antiinfectious agents. Antiparasitic agents Antiparasitic agents Antiprotozoal Agents - pharmacology Biological and medical sciences Electrophoresis, Starch Gel Genetic diversity Genetic heterogeneity Genetic Variation Giardia - drug effects Giardia - enzymology Giardia - genetics Giardia - growth & development Giardia duodenalis Hexokinase - analysis Hexokinase - genetics Human genetics Humans Ice Medical genetics Medical sciences Metronidazole - pharmacology Parasites Parasitology Pharmacology. Drug treatments Phosphoglucomutase - analysis Phosphoglucomutase - genetics Population genetics Research Notes Trophozoites |
| title | Giardia duodenalis: Exposure to Metronidazole Inhibits Competitive Interactions between Isolates of the Parasite In vitro |
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