Effects of brain ischemia on intermediate filaments of rat hippocampus
Neurofilaments subunits (NF-H, NF-M, NF-L) and glial fibrillary acidic protein (GFAP) were investigated in the hippocampus of rats after distinct periods of reperfusion (1 to 15 days) following 20 min of transient global forebrain ischemia in the rat. In vitro [14Ca]leucine incorporation was not alt...
Gespeichert in:
| Veröffentlicht in: | Neurochemical research 1996-05, Vol.21 (5), p.595-602 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 602 |
|---|---|
| container_issue | 5 |
| container_start_page | 595 |
| container_title | Neurochemical research |
| container_volume | 21 |
| creator | CAMARGO-DE-MORAIS, M DE FREITAS, M SOUZA, C. A. B PESSOA-PUREUR, R NETTO, C. A DE MATTOS, A. G SCHRÖDER, N ZILLES, A. C LISBOA, C. S. F ARTENI, N BARLEM, A SCHIERHOLT, R ZWETSCH, G |
| description | Neurofilaments subunits (NF-H, NF-M, NF-L) and glial fibrillary acidic protein (GFAP) were investigated in the hippocampus of rats after distinct periods of reperfusion (1 to 15 days) following 20 min of transient global forebrain ischemia in the rat. In vitro [14Ca]leucine incorporation was not altered until 48 h after the ischemic insult, however concentration of intermediate filament subunits significantly decreased in this period. Three days after the insult, leucine incorporation significantly increased while the concentration NF-H, NF-M, and NF-L were still diminished after 15 days of reperfusion. In vitro incorporation of 32P into NF-M and NF-L suffered immediately after ischemia, but returned to control values after two days of reperfusion. GFAP levels decreased immediately after ischemia but quickly recovered and significantly peaked from 7 to 10 days after the insult. These results suggest that transient ischemia followed by reperfusion causes proteolysis of intermediate filaments in the hippocampus, and the proteolysis could be facilitated by diminished phosphorylation levels of NF-M and NF-L. |
| doi_str_mv | 10.1007/BF02527758 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_78220286</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>78220286</sourcerecordid><originalsourceid>FETCH-LOGICAL-c311t-c46f440b605d540c8eb4a0085b88d0c12b19bf7c455d554935111b69e7a1b3d3</originalsourceid><addsrcrecordid>eNpFkE1LAzEQhoMotVYv3oU9iAdhdbL53KOWVoWCl96XJJvQyH6Z7B7890a61NMMvA8vMw9CtxieMIB4ft1CwQohmDxDS8wEyXkJ5BwtgXCaE1zCJbqK8Qsg4QVeoIUUBS-5XKLtxjlrxpj1LtNB-S7z0Rxs61XWp70bbWht7dVoM-cb1druyAY1Zgc_DL1R7TDFa3ThVBPtzTxXaL_d7Nfv-e7z7WP9sssNwXjMDeWOUtAcWM0oGGk1VQCSaSlrMLjQuNROGMpSzmhJGMZY89IKhTWpyQo9HGuH0H9PNo5Vm861TaM620-xErIooJA8gY9H0IQ-xmBdNQTfqvBTYaj-nFX_zhJ8N7dOOj17QmdJKb-fcxWNalxQnfHxhBGQBMuS_AJ3znH8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>78220286</pqid></control><display><type>article</type><title>Effects of brain ischemia on intermediate filaments of rat hippocampus</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>CAMARGO-DE-MORAIS, M ; DE FREITAS, M ; SOUZA, C. A. B ; PESSOA-PUREUR, R ; NETTO, C. A ; DE MATTOS, A. G ; SCHRÖDER, N ; ZILLES, A. C ; LISBOA, C. S. F ; ARTENI, N ; BARLEM, A ; SCHIERHOLT, R ; ZWETSCH, G</creator><creatorcontrib>CAMARGO-DE-MORAIS, M ; DE FREITAS, M ; SOUZA, C. A. B ; PESSOA-PUREUR, R ; NETTO, C. A ; DE MATTOS, A. G ; SCHRÖDER, N ; ZILLES, A. C ; LISBOA, C. S. F ; ARTENI, N ; BARLEM, A ; SCHIERHOLT, R ; ZWETSCH, G</creatorcontrib><description>Neurofilaments subunits (NF-H, NF-M, NF-L) and glial fibrillary acidic protein (GFAP) were investigated in the hippocampus of rats after distinct periods of reperfusion (1 to 15 days) following 20 min of transient global forebrain ischemia in the rat. In vitro [14Ca]leucine incorporation was not altered until 48 h after the ischemic insult, however concentration of intermediate filament subunits significantly decreased in this period. Three days after the insult, leucine incorporation significantly increased while the concentration NF-H, NF-M, and NF-L were still diminished after 15 days of reperfusion. In vitro incorporation of 32P into NF-M and NF-L suffered immediately after ischemia, but returned to control values after two days of reperfusion. GFAP levels decreased immediately after ischemia but quickly recovered and significantly peaked from 7 to 10 days after the insult. These results suggest that transient ischemia followed by reperfusion causes proteolysis of intermediate filaments in the hippocampus, and the proteolysis could be facilitated by diminished phosphorylation levels of NF-M and NF-L.</description><identifier>ISSN: 0364-3190</identifier><identifier>EISSN: 1573-6903</identifier><identifier>DOI: 10.1007/BF02527758</identifier><identifier>PMID: 8726968</identifier><identifier>CODEN: NEREDZ</identifier><language>eng</language><publisher>New York, NY: Springer</publisher><subject>Animals ; Biological and medical sciences ; Carbon Radioisotopes ; Cytoskeletal Proteins - metabolism ; Glial Fibrillary Acidic Protein - biosynthesis ; Hippocampus - metabolism ; Ischemic Attack, Transient - metabolism ; Kinetics ; Leucine - metabolism ; Medical sciences ; Neurofilament Proteins - metabolism ; Neurology ; Phosphorus Radioisotopes ; Radioisotope Dilution Technique ; Rats ; Rats, Wistar ; Reperfusion ; Time Factors ; Vascular diseases and vascular malformations of the nervous system</subject><ispartof>Neurochemical research, 1996-05, Vol.21 (5), p.595-602</ispartof><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c311t-c46f440b605d540c8eb4a0085b88d0c12b19bf7c455d554935111b69e7a1b3d3</citedby><cites>FETCH-LOGICAL-c311t-c46f440b605d540c8eb4a0085b88d0c12b19bf7c455d554935111b69e7a1b3d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3083189$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8726968$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>CAMARGO-DE-MORAIS, M</creatorcontrib><creatorcontrib>DE FREITAS, M</creatorcontrib><creatorcontrib>SOUZA, C. A. B</creatorcontrib><creatorcontrib>PESSOA-PUREUR, R</creatorcontrib><creatorcontrib>NETTO, C. A</creatorcontrib><creatorcontrib>DE MATTOS, A. G</creatorcontrib><creatorcontrib>SCHRÖDER, N</creatorcontrib><creatorcontrib>ZILLES, A. C</creatorcontrib><creatorcontrib>LISBOA, C. S. F</creatorcontrib><creatorcontrib>ARTENI, N</creatorcontrib><creatorcontrib>BARLEM, A</creatorcontrib><creatorcontrib>SCHIERHOLT, R</creatorcontrib><creatorcontrib>ZWETSCH, G</creatorcontrib><title>Effects of brain ischemia on intermediate filaments of rat hippocampus</title><title>Neurochemical research</title><addtitle>Neurochem Res</addtitle><description>Neurofilaments subunits (NF-H, NF-M, NF-L) and glial fibrillary acidic protein (GFAP) were investigated in the hippocampus of rats after distinct periods of reperfusion (1 to 15 days) following 20 min of transient global forebrain ischemia in the rat. In vitro [14Ca]leucine incorporation was not altered until 48 h after the ischemic insult, however concentration of intermediate filament subunits significantly decreased in this period. Three days after the insult, leucine incorporation significantly increased while the concentration NF-H, NF-M, and NF-L were still diminished after 15 days of reperfusion. In vitro incorporation of 32P into NF-M and NF-L suffered immediately after ischemia, but returned to control values after two days of reperfusion. GFAP levels decreased immediately after ischemia but quickly recovered and significantly peaked from 7 to 10 days after the insult. These results suggest that transient ischemia followed by reperfusion causes proteolysis of intermediate filaments in the hippocampus, and the proteolysis could be facilitated by diminished phosphorylation levels of NF-M and NF-L.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Carbon Radioisotopes</subject><subject>Cytoskeletal Proteins - metabolism</subject><subject>Glial Fibrillary Acidic Protein - biosynthesis</subject><subject>Hippocampus - metabolism</subject><subject>Ischemic Attack, Transient - metabolism</subject><subject>Kinetics</subject><subject>Leucine - metabolism</subject><subject>Medical sciences</subject><subject>Neurofilament Proteins - metabolism</subject><subject>Neurology</subject><subject>Phosphorus Radioisotopes</subject><subject>Radioisotope Dilution Technique</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Reperfusion</subject><subject>Time Factors</subject><subject>Vascular diseases and vascular malformations of the nervous system</subject><issn>0364-3190</issn><issn>1573-6903</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE1LAzEQhoMotVYv3oU9iAdhdbL53KOWVoWCl96XJJvQyH6Z7B7890a61NMMvA8vMw9CtxieMIB4ft1CwQohmDxDS8wEyXkJ5BwtgXCaE1zCJbqK8Qsg4QVeoIUUBS-5XKLtxjlrxpj1LtNB-S7z0Rxs61XWp70bbWht7dVoM-cb1druyAY1Zgc_DL1R7TDFa3ThVBPtzTxXaL_d7Nfv-e7z7WP9sssNwXjMDeWOUtAcWM0oGGk1VQCSaSlrMLjQuNROGMpSzmhJGMZY89IKhTWpyQo9HGuH0H9PNo5Vm861TaM620-xErIooJA8gY9H0IQ-xmBdNQTfqvBTYaj-nFX_zhJ8N7dOOj17QmdJKb-fcxWNalxQnfHxhBGQBMuS_AJ3znH8</recordid><startdate>19960501</startdate><enddate>19960501</enddate><creator>CAMARGO-DE-MORAIS, M</creator><creator>DE FREITAS, M</creator><creator>SOUZA, C. A. B</creator><creator>PESSOA-PUREUR, R</creator><creator>NETTO, C. A</creator><creator>DE MATTOS, A. G</creator><creator>SCHRÖDER, N</creator><creator>ZILLES, A. C</creator><creator>LISBOA, C. S. F</creator><creator>ARTENI, N</creator><creator>BARLEM, A</creator><creator>SCHIERHOLT, R</creator><creator>ZWETSCH, G</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19960501</creationdate><title>Effects of brain ischemia on intermediate filaments of rat hippocampus</title><author>CAMARGO-DE-MORAIS, M ; DE FREITAS, M ; SOUZA, C. A. B ; PESSOA-PUREUR, R ; NETTO, C. A ; DE MATTOS, A. G ; SCHRÖDER, N ; ZILLES, A. C ; LISBOA, C. S. F ; ARTENI, N ; BARLEM, A ; SCHIERHOLT, R ; ZWETSCH, G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c311t-c46f440b605d540c8eb4a0085b88d0c12b19bf7c455d554935111b69e7a1b3d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Carbon Radioisotopes</topic><topic>Cytoskeletal Proteins - metabolism</topic><topic>Glial Fibrillary Acidic Protein - biosynthesis</topic><topic>Hippocampus - metabolism</topic><topic>Ischemic Attack, Transient - metabolism</topic><topic>Kinetics</topic><topic>Leucine - metabolism</topic><topic>Medical sciences</topic><topic>Neurofilament Proteins - metabolism</topic><topic>Neurology</topic><topic>Phosphorus Radioisotopes</topic><topic>Radioisotope Dilution Technique</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Reperfusion</topic><topic>Time Factors</topic><topic>Vascular diseases and vascular malformations of the nervous system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CAMARGO-DE-MORAIS, M</creatorcontrib><creatorcontrib>DE FREITAS, M</creatorcontrib><creatorcontrib>SOUZA, C. A. B</creatorcontrib><creatorcontrib>PESSOA-PUREUR, R</creatorcontrib><creatorcontrib>NETTO, C. A</creatorcontrib><creatorcontrib>DE MATTOS, A. G</creatorcontrib><creatorcontrib>SCHRÖDER, N</creatorcontrib><creatorcontrib>ZILLES, A. C</creatorcontrib><creatorcontrib>LISBOA, C. S. F</creatorcontrib><creatorcontrib>ARTENI, N</creatorcontrib><creatorcontrib>BARLEM, A</creatorcontrib><creatorcontrib>SCHIERHOLT, R</creatorcontrib><creatorcontrib>ZWETSCH, G</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Neurochemical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CAMARGO-DE-MORAIS, M</au><au>DE FREITAS, M</au><au>SOUZA, C. A. B</au><au>PESSOA-PUREUR, R</au><au>NETTO, C. A</au><au>DE MATTOS, A. G</au><au>SCHRÖDER, N</au><au>ZILLES, A. C</au><au>LISBOA, C. S. F</au><au>ARTENI, N</au><au>BARLEM, A</au><au>SCHIERHOLT, R</au><au>ZWETSCH, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of brain ischemia on intermediate filaments of rat hippocampus</atitle><jtitle>Neurochemical research</jtitle><addtitle>Neurochem Res</addtitle><date>1996-05-01</date><risdate>1996</risdate><volume>21</volume><issue>5</issue><spage>595</spage><epage>602</epage><pages>595-602</pages><issn>0364-3190</issn><eissn>1573-6903</eissn><coden>NEREDZ</coden><abstract>Neurofilaments subunits (NF-H, NF-M, NF-L) and glial fibrillary acidic protein (GFAP) were investigated in the hippocampus of rats after distinct periods of reperfusion (1 to 15 days) following 20 min of transient global forebrain ischemia in the rat. In vitro [14Ca]leucine incorporation was not altered until 48 h after the ischemic insult, however concentration of intermediate filament subunits significantly decreased in this period. Three days after the insult, leucine incorporation significantly increased while the concentration NF-H, NF-M, and NF-L were still diminished after 15 days of reperfusion. In vitro incorporation of 32P into NF-M and NF-L suffered immediately after ischemia, but returned to control values after two days of reperfusion. GFAP levels decreased immediately after ischemia but quickly recovered and significantly peaked from 7 to 10 days after the insult. These results suggest that transient ischemia followed by reperfusion causes proteolysis of intermediate filaments in the hippocampus, and the proteolysis could be facilitated by diminished phosphorylation levels of NF-M and NF-L.</abstract><cop>New York, NY</cop><pub>Springer</pub><pmid>8726968</pmid><doi>10.1007/BF02527758</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0364-3190 |
| ispartof | Neurochemical research, 1996-05, Vol.21 (5), p.595-602 |
| issn | 0364-3190 1573-6903 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78220286 |
| source | MEDLINE; SpringerLink Journals |
| subjects | Animals Biological and medical sciences Carbon Radioisotopes Cytoskeletal Proteins - metabolism Glial Fibrillary Acidic Protein - biosynthesis Hippocampus - metabolism Ischemic Attack, Transient - metabolism Kinetics Leucine - metabolism Medical sciences Neurofilament Proteins - metabolism Neurology Phosphorus Radioisotopes Radioisotope Dilution Technique Rats Rats, Wistar Reperfusion Time Factors Vascular diseases and vascular malformations of the nervous system |
| title | Effects of brain ischemia on intermediate filaments of rat hippocampus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T20%3A48%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effects%20of%20brain%20ischemia%20on%20intermediate%20filaments%20of%20rat%20hippocampus&rft.jtitle=Neurochemical%20research&rft.au=CAMARGO-DE-MORAIS,%20M&rft.date=1996-05-01&rft.volume=21&rft.issue=5&rft.spage=595&rft.epage=602&rft.pages=595-602&rft.issn=0364-3190&rft.eissn=1573-6903&rft.coden=NEREDZ&rft_id=info:doi/10.1007/BF02527758&rft_dat=%3Cproquest_cross%3E78220286%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=78220286&rft_id=info:pmid/8726968&rfr_iscdi=true |