Sequence variability and function of measles virus 3′ and 5′ ends and intercistronic regions
Sequences critical for the activity of the measles virus (MV) RNA polymerase in transcription and replication were analyzed using a MV genomic cDNA library containing overlapping clones encompassing the entire MV genome. Clones corresponding to the 3′ and 5′ ends of the MV genome were identified and...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1988-06, Vol.164 (2), p.498-506 |
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| container_title | Virology (New York, N.Y.) |
| container_volume | 164 |
| creator | Crowley, Joan C. Dowling, Peter C. Menonna, Joseph Silverman, Joel I. Schuback, Deborah Cook, Stuart D. Blumberg, Benjamin M. |
| description | Sequences critical for the activity of the measles virus (MV) RNA polymerase in transcription and replication were analyzed using a MV genomic cDNA library containing overlapping clones encompassing the entire MV genome. Clones corresponding to the 3′ and 5′ ends of the MV genome were identified and sequenced, and these sequences were confirmed by primer extension experiments. Neither (+) nor (−) strand leader RNAs were detected in MV-infected cell extracts, using high specific activity riboprobes made from these clones. Clones representing each of the MV gene boundaries were also sequenced, and variations including point mutations, insertions, and deletions were noted. Together with the sequence of the MV L gene region, this report completes the sequence determination of the MV genome. |
| doi_str_mv | 10.1016/0042-6822(88)90564-8 |
| format | Article |
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Clones corresponding to the 3′ and 5′ ends of the MV genome were identified and sequenced, and these sequences were confirmed by primer extension experiments. Neither (+) nor (−) strand leader RNAs were detected in MV-infected cell extracts, using high specific activity riboprobes made from these clones. Clones representing each of the MV gene boundaries were also sequenced, and variations including point mutations, insertions, and deletions were noted. 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Clones corresponding to the 3′ and 5′ ends of the MV genome were identified and sequenced, and these sequences were confirmed by primer extension experiments. Neither (+) nor (−) strand leader RNAs were detected in MV-infected cell extracts, using high specific activity riboprobes made from these clones. Clones representing each of the MV gene boundaries were also sequenced, and variations including point mutations, insertions, and deletions were noted. Together with the sequence of the MV L gene region, this report completes the sequence determination of the MV genome.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>DNA - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Viral</subject><subject>Genetic Variation</subject><subject>Genetics</subject><subject>Hydrogen Bonding</subject><subject>measles virus</subject><subject>Measles virus - genetics</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Conformation</subject><subject>RNA, Viral - genetics</subject><subject>Virology</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc-qEzEUh4Motbf6BgqzELkuRk8maf5sBCn3qlBwoa5jJnMikWmmJjOF7nwmH8knMdOWLnWVHM53Dsn3I-QZhdcUqHgDwJtaqKa5VeqVhrXgtXpAlhS0qIFx-pAsr8hjcpPzDyi1lLAgC8aEBg1L8u0z_pwwOqwONgXbhj6Mx8rGrvJTdGMYYjX4aoc295irQ0hTrtifX79PyHq-YOzyqQpxxORCHtMQg6sSfi_T-Ql55G2f8enlXJGv93dfNh_q7af3HzfvtrXjVI61ogxbJyi1yJltfEMdAjgrwbK2YYpzzyjtAK0THloB3mtsdOsYcNFJy1bk5XnvPg3lR3k0u5Ad9r2NOEzZyGIBqOT_BSnXQjCxLiA_gy4NOSf0Zp_CzqajoWDmBMys18x6jVLmlIBRZez5Zf_U7rC7Dl2Ul_6LS99mZ3ufbCzSrpiUWq8bWbC3ZwyLtEPAZLILc1BdSOhG0w3h3-_4C5GFpAI</recordid><startdate>19880601</startdate><enddate>19880601</enddate><creator>Crowley, Joan C.</creator><creator>Dowling, Peter C.</creator><creator>Menonna, Joseph</creator><creator>Silverman, Joel I.</creator><creator>Schuback, Deborah</creator><creator>Cook, Stuart D.</creator><creator>Blumberg, Benjamin M.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19880601</creationdate><title>Sequence variability and function of measles virus 3′ and 5′ ends and intercistronic regions</title><author>Crowley, Joan C. ; Dowling, Peter C. ; Menonna, Joseph ; Silverman, Joel I. ; Schuback, Deborah ; Cook, Stuart D. ; Blumberg, Benjamin M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-813ebc611ae43a2f21ce00ca70a3b23844f311d0eac6f0b60ff9e29bc3046d7a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cloning, Molecular</topic><topic>DNA - genetics</topic><topic>Fundamental and applied biological sciences. 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| identifier | ISSN: 0042-6822 |
| ispartof | Virology (New York, N.Y.), 1988-06, Vol.164 (2), p.498-506 |
| issn | 0042-6822 1096-0341 |
| language | eng |
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| source | MEDLINE; Free E-Journal (出版社公開部分のみ）; Elsevier ScienceDirect Journals Collection |
| subjects | Base Sequence Biological and medical sciences Cloning, Molecular DNA - genetics Fundamental and applied biological sciences. Psychology Genes, Viral Genetic Variation Genetics Hydrogen Bonding measles virus Measles virus - genetics Microbiology Molecular Sequence Data Nucleic Acid Conformation RNA, Viral - genetics Virology |
| title | Sequence variability and function of measles virus 3′ and 5′ ends and intercistronic regions |
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