Echinococcus multilocularis: Comparative study of glycogen synthase in metacestodes and in the livers of infected and control Meriones unguiculatus
A comparative study of the kinetic parameters of glycogen synthase was performed on Echinococcus multilocularis metacestodes and on the livers of infected and control host ( Meriones unguiculatus). The enzyme of the parasite was found to be different from the enzyme of infected host liver. The appar...
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| Veröffentlicht in: | Experimental parasitology 1988-06, Vol.66 (1), p.1-6 |
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| creator | Sarciron, M.Elisabeth Azzar, Gérard Got, René Petavy, Anne-Françoise |
| description | A comparative study of the kinetic parameters of glycogen synthase was performed on
Echinococcus multilocularis metacestodes and on the livers of infected and control host (
Meriones unguiculatus). The enzyme of the parasite was found to be different from the enzyme of infected host liver. The apparent
K
m
for UDP-glucose is 100 μ
M for the parasite and 400 μ
M for the host liver. The apparent
K
m
for glucose 6-phosphate is 4 m
M for the parasite and 2 m
M for the host liver. The apparent
K
m
for glycogen is 16 mg/ml for the parasite and 125 mg/ml for the host liver. The influence of glucose 6-phosphate and exogenous glycogen on the activity of glycogen synthase differs between the metacestode and the host liver. The enzyme of the metacestodes apparently does not need exogenous glycogen to work, contrary to the case for the liver host enzyme. The glycogen synthase of the parasite seems to be present in forms I and D, whereas the enzyme of the host liver appears in form I and that of the control liver in form D. |
| doi_str_mv | 10.1016/0014-4894(88)90043-4 |
| format | Article |
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Echinococcus multilocularis metacestodes and on the livers of infected and control host (
Meriones unguiculatus). The enzyme of the parasite was found to be different from the enzyme of infected host liver. The apparent
K
m
for UDP-glucose is 100 μ
M for the parasite and 400 μ
M for the host liver. The apparent
K
m
for glucose 6-phosphate is 4 m
M for the parasite and 2 m
M for the host liver. The apparent
K
m
for glycogen is 16 mg/ml for the parasite and 125 mg/ml for the host liver. The influence of glucose 6-phosphate and exogenous glycogen on the activity of glycogen synthase differs between the metacestode and the host liver. The enzyme of the metacestodes apparently does not need exogenous glycogen to work, contrary to the case for the liver host enzyme. The glycogen synthase of the parasite seems to be present in forms I and D, whereas the enzyme of the host liver appears in form I and that of the control liver in form D.</description><identifier>ISSN: 0014-4894</identifier><identifier>EISSN: 1090-2449</identifier><identifier>DOI: 10.1016/0014-4894(88)90043-4</identifier><identifier>PMID: 3130271</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cestode ; Chemical Phenomena ; Chemistry ; Echinococcosis - enzymology ; Echinococcosis - metabolism ; Echinococcus - analysis ; Echinococcus - enzymology ; Echinococcus - metabolism ; Echinococcus multilocularis ; Enzyme ; Gerbillinae ; Glucose 6-phosphate ; Glucosephosphates - metabolism ; Glycogen ; Glycogen - analysis ; Glycogen - biosynthesis ; Glycogen synthase ; Glycogen Synthase - metabolism ; Kinetics ; Liver ; Liver - analysis ; Liver - enzymology ; Liver - metabolism ; Meriones unguiculatus ; Uridine Diphosphate Glucose - metabolism</subject><ispartof>Experimental parasitology, 1988-06, Vol.66 (1), p.1-6</ispartof><rights>1988</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-2c46fdddc15830f82cbad30c7a3d21cd32bf3c8d84d86d4bdfe019382e06887f3</citedby><cites>FETCH-LOGICAL-c357t-2c46fdddc15830f82cbad30c7a3d21cd32bf3c8d84d86d4bdfe019382e06887f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-4894(88)90043-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3130271$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sarciron, M.Elisabeth</creatorcontrib><creatorcontrib>Azzar, Gérard</creatorcontrib><creatorcontrib>Got, René</creatorcontrib><creatorcontrib>Petavy, Anne-Françoise</creatorcontrib><title>Echinococcus multilocularis: Comparative study of glycogen synthase in metacestodes and in the livers of infected and control Meriones unguiculatus</title><title>Experimental parasitology</title><addtitle>Exp Parasitol</addtitle><description>A comparative study of the kinetic parameters of glycogen synthase was performed on
Echinococcus multilocularis metacestodes and on the livers of infected and control host (
Meriones unguiculatus). The enzyme of the parasite was found to be different from the enzyme of infected host liver. The apparent
K
m
for UDP-glucose is 100 μ
M for the parasite and 400 μ
M for the host liver. The apparent
K
m
for glucose 6-phosphate is 4 m
M for the parasite and 2 m
M for the host liver. The apparent
K
m
for glycogen is 16 mg/ml for the parasite and 125 mg/ml for the host liver. The influence of glucose 6-phosphate and exogenous glycogen on the activity of glycogen synthase differs between the metacestode and the host liver. The enzyme of the metacestodes apparently does not need exogenous glycogen to work, contrary to the case for the liver host enzyme. The glycogen synthase of the parasite seems to be present in forms I and D, whereas the enzyme of the host liver appears in form I and that of the control liver in form D.</description><subject>Animals</subject><subject>Cestode</subject><subject>Chemical Phenomena</subject><subject>Chemistry</subject><subject>Echinococcosis - enzymology</subject><subject>Echinococcosis - metabolism</subject><subject>Echinococcus - analysis</subject><subject>Echinococcus - enzymology</subject><subject>Echinococcus - metabolism</subject><subject>Echinococcus multilocularis</subject><subject>Enzyme</subject><subject>Gerbillinae</subject><subject>Glucose 6-phosphate</subject><subject>Glucosephosphates - metabolism</subject><subject>Glycogen</subject><subject>Glycogen - analysis</subject><subject>Glycogen - biosynthesis</subject><subject>Glycogen synthase</subject><subject>Glycogen Synthase - metabolism</subject><subject>Kinetics</subject><subject>Liver</subject><subject>Liver - analysis</subject><subject>Liver - enzymology</subject><subject>Liver - metabolism</subject><subject>Meriones unguiculatus</subject><subject>Uridine Diphosphate Glucose - metabolism</subject><issn>0014-4894</issn><issn>1090-2449</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1vEzEQhi0EKqHwD0DyCcFhwV_NejkgoagtSEVc4Gw549nEyGsHf1TK7-APs9tEPXIaad73mbHfIeQ1Zx844-uPjHHVKT2od1q_HxhTslNPyIqzgXVCqeEpWT1anpMXpfxmjGku1AW5kFwy0fMV-XsNex8TJIBW6NRC9SFBCzb78olu0nSw2VZ_j7TU5o40jXQXjpB2GGk5xrq3BamPdMJqAUtNDgu10S29ukcaZjSXBfNxRKjoHlRIseYU6HfMPsUZaXHX_LK3tvKSPBttKPjqXC_Jr5vrn5uv3d2P22-bL3cdyKu-dgLUenTOAb_Sko1awNY6yaC30gkOTortKEE7rZxeO7V1IzI-SC2QrbXuR3lJ3p7mHnL60-bHm8kXwBBsxNSK6bXgXEg9G9XJCDmVknE0h-wnm4-GM7PcwixBmyVoo7V5uIVRM_bmPL9tJ3SP0Dn8Wf980nH-5L3HbAp4jIDO5zkq45L__4J_hHqc_w</recordid><startdate>19880601</startdate><enddate>19880601</enddate><creator>Sarciron, M.Elisabeth</creator><creator>Azzar, Gérard</creator><creator>Got, René</creator><creator>Petavy, Anne-Françoise</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19880601</creationdate><title>Echinococcus multilocularis: Comparative study of glycogen synthase in metacestodes and in the livers of infected and control Meriones unguiculatus</title><author>Sarciron, M.Elisabeth ; Azzar, Gérard ; Got, René ; Petavy, Anne-Françoise</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-2c46fdddc15830f82cbad30c7a3d21cd32bf3c8d84d86d4bdfe019382e06887f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Animals</topic><topic>Cestode</topic><topic>Chemical Phenomena</topic><topic>Chemistry</topic><topic>Echinococcosis - enzymology</topic><topic>Echinococcosis - metabolism</topic><topic>Echinococcus - analysis</topic><topic>Echinococcus - enzymology</topic><topic>Echinococcus - metabolism</topic><topic>Echinococcus multilocularis</topic><topic>Enzyme</topic><topic>Gerbillinae</topic><topic>Glucose 6-phosphate</topic><topic>Glucosephosphates - metabolism</topic><topic>Glycogen</topic><topic>Glycogen - analysis</topic><topic>Glycogen - biosynthesis</topic><topic>Glycogen synthase</topic><topic>Glycogen Synthase - metabolism</topic><topic>Kinetics</topic><topic>Liver</topic><topic>Liver - analysis</topic><topic>Liver - enzymology</topic><topic>Liver - metabolism</topic><topic>Meriones unguiculatus</topic><topic>Uridine Diphosphate Glucose - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sarciron, M.Elisabeth</creatorcontrib><creatorcontrib>Azzar, Gérard</creatorcontrib><creatorcontrib>Got, René</creatorcontrib><creatorcontrib>Petavy, Anne-Françoise</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sarciron, M.Elisabeth</au><au>Azzar, Gérard</au><au>Got, René</au><au>Petavy, Anne-Françoise</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Echinococcus multilocularis: Comparative study of glycogen synthase in metacestodes and in the livers of infected and control Meriones unguiculatus</atitle><jtitle>Experimental parasitology</jtitle><addtitle>Exp Parasitol</addtitle><date>1988-06-01</date><risdate>1988</risdate><volume>66</volume><issue>1</issue><spage>1</spage><epage>6</epage><pages>1-6</pages><issn>0014-4894</issn><eissn>1090-2449</eissn><abstract>A comparative study of the kinetic parameters of glycogen synthase was performed on
Echinococcus multilocularis metacestodes and on the livers of infected and control host (
Meriones unguiculatus). The enzyme of the parasite was found to be different from the enzyme of infected host liver. The apparent
K
m
for UDP-glucose is 100 μ
M for the parasite and 400 μ
M for the host liver. The apparent
K
m
for glucose 6-phosphate is 4 m
M for the parasite and 2 m
M for the host liver. The apparent
K
m
for glycogen is 16 mg/ml for the parasite and 125 mg/ml for the host liver. The influence of glucose 6-phosphate and exogenous glycogen on the activity of glycogen synthase differs between the metacestode and the host liver. The enzyme of the metacestodes apparently does not need exogenous glycogen to work, contrary to the case for the liver host enzyme. The glycogen synthase of the parasite seems to be present in forms I and D, whereas the enzyme of the host liver appears in form I and that of the control liver in form D.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>3130271</pmid><doi>10.1016/0014-4894(88)90043-4</doi><tpages>6</tpages></addata></record> |
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| ispartof | Experimental parasitology, 1988-06, Vol.66 (1), p.1-6 |
| issn | 0014-4894 1090-2449 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Cestode Chemical Phenomena Chemistry Echinococcosis - enzymology Echinococcosis - metabolism Echinococcus - analysis Echinococcus - enzymology Echinococcus - metabolism Echinococcus multilocularis Enzyme Gerbillinae Glucose 6-phosphate Glucosephosphates - metabolism Glycogen Glycogen - analysis Glycogen - biosynthesis Glycogen synthase Glycogen Synthase - metabolism Kinetics Liver Liver - analysis Liver - enzymology Liver - metabolism Meriones unguiculatus Uridine Diphosphate Glucose - metabolism |
| title | Echinococcus multilocularis: Comparative study of glycogen synthase in metacestodes and in the livers of infected and control Meriones unguiculatus |
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