Langerhans cell density and serological changes following intradermal immunisation of mice with dengue 2 virus
1 Department of Pathobiology, Faculty of Science, Mahidol University, Rama 6 Road * Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Rama 6 Road, Bangkok 10400 Center for Vaccine Development, Institute of Science and Technology for Development, Mahidol University at Salaya, Nakhon...
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| Veröffentlicht in: | Journal of medical microbiology 1996-08, Vol.45 (2), p.138-145 |
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| creator | TAWEECHAISUPAPONG, SUWIMOL SRIURAIRATANA, SIRIPORN ANGSUBHAKORN, SUBHKIJ YOKSAN, SUTEE KHIN, MI MI SAHAPHONG, SOMPHONG BHAMARAPRAVATI, NATTH |
| description | 1 Department of Pathobiology, Faculty of Science, Mahidol University, Rama 6 Road
* Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Rama 6 Road, Bangkok 10400
Center for Vaccine Development, Institute of Science and Technology for Development, Mahidol University at Salaya, Nakhon Pathom 73170, Thailand
Corresponding author: Professor S. Angsubhakorn.
Received May 1, 1995
Accepted December 22, 1995
After the introduction of the dengue-2 (16681) virus by intradermal (i.d.) injection into the footpads of mice, Langerhans cells (LCs) increased in numbers within 24 h at the site of injection and neutralising antibody developed. On comparing the i.d. and intramuscular (i.m.) routes, antibody was produced more rapidly and at higher levels when the virus was injected by the i.d. route. Subsequent re-challenge by the i.d. route produced an even more rapid serological response with all mice producing significant neutralising titres within 12 h. Numbers of ATPase-positive LCs varied with time. A significant sharp drop in LC densities in the early post-injection phase directly correlated with the increased numbers of dendritic cells in the superficial dermis and interfollicular sinuses of draining lymph nodes (LN). Immunofluorescence showed the presence of viral antigen in the footpad epidermis and draining LN within minutes or within 2 h of challenge, respectively. |
| doi_str_mv | 10.1099/00222615-45-2-138 |
| format | Article |
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* Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Rama 6 Road, Bangkok 10400
Center for Vaccine Development, Institute of Science and Technology for Development, Mahidol University at Salaya, Nakhon Pathom 73170, Thailand
Corresponding author: Professor S. Angsubhakorn.
Received May 1, 1995
Accepted December 22, 1995
After the introduction of the dengue-2 (16681) virus by intradermal (i.d.) injection into the footpads of mice, Langerhans cells (LCs) increased in numbers within 24 h at the site of injection and neutralising antibody developed. On comparing the i.d. and intramuscular (i.m.) routes, antibody was produced more rapidly and at higher levels when the virus was injected by the i.d. route. Subsequent re-challenge by the i.d. route produced an even more rapid serological response with all mice producing significant neutralising titres within 12 h. Numbers of ATPase-positive LCs varied with time. A significant sharp drop in LC densities in the early post-injection phase directly correlated with the increased numbers of dendritic cells in the superficial dermis and interfollicular sinuses of draining lymph nodes (LN). Immunofluorescence showed the presence of viral antigen in the footpad epidermis and draining LN within minutes or within 2 h of challenge, respectively.</description><identifier>ISSN: 0022-2615</identifier><identifier>EISSN: 1473-5644</identifier><identifier>DOI: 10.1099/00222615-45-2-138</identifier><identifier>PMID: 8683550</identifier><identifier>CODEN: JMMIAV</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Analysis of the immune response. Humoral and cellular immunity ; Animals ; Antibodies, Viral - biosynthesis ; Antigens, Viral - analysis ; Biological and medical sciences ; Cell Count ; Dengue Virus - immunology ; dengue virus 2 ; Epidermis - cytology ; Epidermis - immunology ; Foot ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Immunity, Cellular ; Immunization, Secondary ; Immunobiology ; Injections, Intradermal ; Langerhans Cells - cytology ; Langerhans Cells - immunology ; Lymph Nodes - immunology ; Lymph Nodes - pathology ; Male ; Mice ; Mice, Inbred BALB C ; Neutralization Tests ; Organs and cells involved in the immune response ; Vaccines, Attenuated - administration & dosage ; Vaccines, Attenuated - immunology ; Viral Vaccines - administration & dosage ; Viral Vaccines - immunology</subject><ispartof>Journal of medical microbiology, 1996-08, Vol.45 (2), p.138-145</ispartof><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c431t-e134b157ec2f4baa9bbc00acab0872f7e31072fc57f097b961680d72ab0d56b63</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3746,3747,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3170029$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8683550$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>TAWEECHAISUPAPONG, SUWIMOL</creatorcontrib><creatorcontrib>SRIURAIRATANA, SIRIPORN</creatorcontrib><creatorcontrib>ANGSUBHAKORN, SUBHKIJ</creatorcontrib><creatorcontrib>YOKSAN, SUTEE</creatorcontrib><creatorcontrib>KHIN, MI MI</creatorcontrib><creatorcontrib>SAHAPHONG, SOMPHONG</creatorcontrib><creatorcontrib>BHAMARAPRAVATI, NATTH</creatorcontrib><title>Langerhans cell density and serological changes following intradermal immunisation of mice with dengue 2 virus</title><title>Journal of medical microbiology</title><addtitle>J Med Microbiol</addtitle><description>1 Department of Pathobiology, Faculty of Science, Mahidol University, Rama 6 Road
* Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Rama 6 Road, Bangkok 10400
Center for Vaccine Development, Institute of Science and Technology for Development, Mahidol University at Salaya, Nakhon Pathom 73170, Thailand
Corresponding author: Professor S. Angsubhakorn.
Received May 1, 1995
Accepted December 22, 1995
After the introduction of the dengue-2 (16681) virus by intradermal (i.d.) injection into the footpads of mice, Langerhans cells (LCs) increased in numbers within 24 h at the site of injection and neutralising antibody developed. On comparing the i.d. and intramuscular (i.m.) routes, antibody was produced more rapidly and at higher levels when the virus was injected by the i.d. route. Subsequent re-challenge by the i.d. route produced an even more rapid serological response with all mice producing significant neutralising titres within 12 h. Numbers of ATPase-positive LCs varied with time. A significant sharp drop in LC densities in the early post-injection phase directly correlated with the increased numbers of dendritic cells in the superficial dermis and interfollicular sinuses of draining lymph nodes (LN). Immunofluorescence showed the presence of viral antigen in the footpad epidermis and draining LN within minutes or within 2 h of challenge, respectively.</description><subject>Analysis of the immune response. Humoral and cellular immunity</subject><subject>Animals</subject><subject>Antibodies, Viral - biosynthesis</subject><subject>Antigens, Viral - analysis</subject><subject>Biological and medical sciences</subject><subject>Cell Count</subject><subject>Dengue Virus - immunology</subject><subject>dengue virus 2</subject><subject>Epidermis - cytology</subject><subject>Epidermis - immunology</subject><subject>Foot</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Immunity, Cellular</subject><subject>Immunization, Secondary</subject><subject>Immunobiology</subject><subject>Injections, Intradermal</subject><subject>Langerhans Cells - cytology</subject><subject>Langerhans Cells - immunology</subject><subject>Lymph Nodes - immunology</subject><subject>Lymph Nodes - pathology</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Neutralization Tests</subject><subject>Organs and cells involved in the immune response</subject><subject>Vaccines, Attenuated - administration & dosage</subject><subject>Vaccines, Attenuated - immunology</subject><subject>Viral Vaccines - administration & dosage</subject><subject>Viral Vaccines - immunology</subject><issn>0022-2615</issn><issn>1473-5644</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUuP1DAQhC0EWoaFH8AByQcEp4AfsZ0c0YqXNBIXOFu200m8iu3FThjtv8fRDHPl1If6ulpdhdBrSj5Q0vcfCWGMSSqaVjSsobx7gg60VbwRsm2fosOuNzvwHL0o5Z4Qqjjvb9BNJzsuBDmgeDRxgjybWLCDZcEDxOLXR2zigAvktKTJO7NgN-9gwWNalnTyccI-rtkMkENVfQhb9MWsPkWcRhy8A3zy67z7TRtghv_4vJWX6NlolgKvLvMW_fry-efdt-b44-v3u0_HxrWcrg1Q3loqFDg2ttaY3lpHiHHGkk6xUQGnpE4n1Eh6ZXtJZUcGxao-CGklv0Xvzr4POf3eoKw6-LL_ZyKkrWjVMSIYE_8FqZCKUNlXkJ5Bl1MpGUb9kH0w-VFTovcy9L8ydCs007WMuvPmYr7ZAMN145J-1d9edFNqxmM20flyxThV1XM__f6MzX6aTz6DniDWhHOyPun7EK4H_wJpD6CM</recordid><startdate>19960801</startdate><enddate>19960801</enddate><creator>TAWEECHAISUPAPONG, SUWIMOL</creator><creator>SRIURAIRATANA, SIRIPORN</creator><creator>ANGSUBHAKORN, SUBHKIJ</creator><creator>YOKSAN, SUTEE</creator><creator>KHIN, MI MI</creator><creator>SAHAPHONG, SOMPHONG</creator><creator>BHAMARAPRAVATI, NATTH</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19960801</creationdate><title>Langerhans cell density and serological changes following intradermal immunisation of mice with dengue 2 virus</title><author>TAWEECHAISUPAPONG, SUWIMOL ; SRIURAIRATANA, SIRIPORN ; ANGSUBHAKORN, SUBHKIJ ; YOKSAN, SUTEE ; KHIN, MI MI ; SAHAPHONG, SOMPHONG ; BHAMARAPRAVATI, NATTH</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-e134b157ec2f4baa9bbc00acab0872f7e31072fc57f097b961680d72ab0d56b63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Analysis of the immune response. 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Psychology</topic><topic>Fundamental immunology</topic><topic>Immunity, Cellular</topic><topic>Immunization, Secondary</topic><topic>Immunobiology</topic><topic>Injections, Intradermal</topic><topic>Langerhans Cells - cytology</topic><topic>Langerhans Cells - immunology</topic><topic>Lymph Nodes - immunology</topic><topic>Lymph Nodes - pathology</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Neutralization Tests</topic><topic>Organs and cells involved in the immune response</topic><topic>Vaccines, Attenuated - administration & dosage</topic><topic>Vaccines, Attenuated - immunology</topic><topic>Viral Vaccines - administration & dosage</topic><topic>Viral Vaccines - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>TAWEECHAISUPAPONG, SUWIMOL</creatorcontrib><creatorcontrib>SRIURAIRATANA, SIRIPORN</creatorcontrib><creatorcontrib>ANGSUBHAKORN, SUBHKIJ</creatorcontrib><creatorcontrib>YOKSAN, SUTEE</creatorcontrib><creatorcontrib>KHIN, MI MI</creatorcontrib><creatorcontrib>SAHAPHONG, SOMPHONG</creatorcontrib><creatorcontrib>BHAMARAPRAVATI, NATTH</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TAWEECHAISUPAPONG, SUWIMOL</au><au>SRIURAIRATANA, SIRIPORN</au><au>ANGSUBHAKORN, SUBHKIJ</au><au>YOKSAN, SUTEE</au><au>KHIN, MI MI</au><au>SAHAPHONG, SOMPHONG</au><au>BHAMARAPRAVATI, NATTH</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Langerhans cell density and serological changes following intradermal immunisation of mice with dengue 2 virus</atitle><jtitle>Journal of medical microbiology</jtitle><addtitle>J Med Microbiol</addtitle><date>1996-08-01</date><risdate>1996</risdate><volume>45</volume><issue>2</issue><spage>138</spage><epage>145</epage><pages>138-145</pages><issn>0022-2615</issn><eissn>1473-5644</eissn><coden>JMMIAV</coden><abstract>1 Department of Pathobiology, Faculty of Science, Mahidol University, Rama 6 Road
* Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Rama 6 Road, Bangkok 10400
Center for Vaccine Development, Institute of Science and Technology for Development, Mahidol University at Salaya, Nakhon Pathom 73170, Thailand
Corresponding author: Professor S. Angsubhakorn.
Received May 1, 1995
Accepted December 22, 1995
After the introduction of the dengue-2 (16681) virus by intradermal (i.d.) injection into the footpads of mice, Langerhans cells (LCs) increased in numbers within 24 h at the site of injection and neutralising antibody developed. On comparing the i.d. and intramuscular (i.m.) routes, antibody was produced more rapidly and at higher levels when the virus was injected by the i.d. route. Subsequent re-challenge by the i.d. route produced an even more rapid serological response with all mice producing significant neutralising titres within 12 h. Numbers of ATPase-positive LCs varied with time. A significant sharp drop in LC densities in the early post-injection phase directly correlated with the increased numbers of dendritic cells in the superficial dermis and interfollicular sinuses of draining lymph nodes (LN). Immunofluorescence showed the presence of viral antigen in the footpad epidermis and draining LN within minutes or within 2 h of challenge, respectively.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>8683550</pmid><doi>10.1099/00222615-45-2-138</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of medical microbiology, 1996-08, Vol.45 (2), p.138-145 |
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| language | eng |
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| source | MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Analysis of the immune response. Humoral and cellular immunity Animals Antibodies, Viral - biosynthesis Antigens, Viral - analysis Biological and medical sciences Cell Count Dengue Virus - immunology dengue virus 2 Epidermis - cytology Epidermis - immunology Foot Fundamental and applied biological sciences. Psychology Fundamental immunology Immunity, Cellular Immunization, Secondary Immunobiology Injections, Intradermal Langerhans Cells - cytology Langerhans Cells - immunology Lymph Nodes - immunology Lymph Nodes - pathology Male Mice Mice, Inbred BALB C Neutralization Tests Organs and cells involved in the immune response Vaccines, Attenuated - administration & dosage Vaccines, Attenuated - immunology Viral Vaccines - administration & dosage Viral Vaccines - immunology |
| title | Langerhans cell density and serological changes following intradermal immunisation of mice with dengue 2 virus |
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