Nitric Oxide: A Mediator of Endotoxin-Induced Middle Ear Effusions
Using a rat model, the authors investigated the role of nitric oxide (NO) in endotoxin‐induced middle ear effusion (MEE). After the eustachian tube was obstructed, the middle ear was transtympanically injected with 35μL of either 1 mg/mL lipopolysaccharide (LPS) or LPS and 1 mmol/L N‐nitro‐L‐arginin...
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| Veröffentlicht in: | The Laryngoscope 1996-08, Vol.106 (8), p.1021-1027 |
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| container_issue | 8 |
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| container_title | The Laryngoscope |
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| creator | Ball, Steven S. Prazma, Jiri Dais, C.G. Dean Rosbe, Kristina W. Pillsbury, Harold C. |
| description | Using a rat model, the authors investigated the role of nitric oxide (NO) in endotoxin‐induced middle ear effusion (MEE). After the eustachian tube was obstructed, the middle ear was transtympanically injected with 35μL of either 1 mg/mL lipopolysaccharide (LPS) or LPS and 1 mmol/L N‐nitro‐L‐arginine methyl ester (L‐NAME), a competitive inhibitor of NO synthase. Over the next 6 hours, the fluid within the middle ear was collected every 2 hours, and the quantity of albumin in the fluid, an index of vascular leakage, was determined using enzyme‐linked immunosorbent assay.
L‐NAME significantly reduced LPS‐induced vascular extravasation into the middle ear. Inoculation of the ear with L‐arginine, the substrate for NO synthase, reversed the effects of L‐NAME. These results indicate that NO is a mediator of LPS‐induced MEE. Therefore, inhibition of NO synthase may represent a novel approach to the treatment of otitis media with effusion. |
| doi_str_mv | 10.1097/00005537-199608000-00022 |
| format | Article |
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L‐NAME significantly reduced LPS‐induced vascular extravasation into the middle ear. Inoculation of the ear with L‐arginine, the substrate for NO synthase, reversed the effects of L‐NAME. These results indicate that NO is a mediator of LPS‐induced MEE. Therefore, inhibition of NO synthase may represent a novel approach to the treatment of otitis media with effusion.</description><identifier>ISSN: 0023-852X</identifier><identifier>EISSN: 1531-4995</identifier><identifier>DOI: 10.1097/00005537-199608000-00022</identifier><identifier>PMID: 8699894</identifier><identifier>CODEN: LARYA8</identifier><language>eng</language><publisher>Hoboken, NJ: John Wiley & Sons, Inc</publisher><subject>Albumins - analysis ; Animals ; Arginine - administration & dosage ; Arginine - analogs & derivatives ; Biological and medical sciences ; Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology ; Enzyme Inhibitors - administration & dosage ; Enzyme-Linked Immunosorbent Assay ; Lipopolysaccharides - administration & dosage ; Medical sciences ; NG-Nitroarginine Methyl Ester ; Nitric Oxide - physiology ; Nitric Oxide Synthase - antagonists & inhibitors ; Non tumoral diseases ; Otitis Media with Effusion - drug therapy ; Otitis Media with Effusion - pathology ; Otitis Media with Effusion - physiopathology ; Otorhinolaryngology. Stomatology ; Rats ; Time Factors</subject><ispartof>The Laryngoscope, 1996-08, Vol.106 (8), p.1021-1027</ispartof><rights>Copyright © 1996 The Triological Society</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4672-af2fb690c8d3b57472a5d0c3ca4826f427d09a14ce8ec9cf2825bea5ef5b465f3</citedby><cites>FETCH-LOGICAL-c4672-af2fb690c8d3b57472a5d0c3ca4826f427d09a14ce8ec9cf2825bea5ef5b465f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>309,310,314,776,780,785,786,23910,23911,25119,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3189039$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8699894$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ball, Steven S.</creatorcontrib><creatorcontrib>Prazma, Jiri</creatorcontrib><creatorcontrib>Dais, C.G. Dean</creatorcontrib><creatorcontrib>Rosbe, Kristina W.</creatorcontrib><creatorcontrib>Pillsbury, Harold C.</creatorcontrib><title>Nitric Oxide: A Mediator of Endotoxin-Induced Middle Ear Effusions</title><title>The Laryngoscope</title><addtitle>The Laryngoscope</addtitle><description>Using a rat model, the authors investigated the role of nitric oxide (NO) in endotoxin‐induced middle ear effusion (MEE). After the eustachian tube was obstructed, the middle ear was transtympanically injected with 35μL of either 1 mg/mL lipopolysaccharide (LPS) or LPS and 1 mmol/L N‐nitro‐L‐arginine methyl ester (L‐NAME), a competitive inhibitor of NO synthase. Over the next 6 hours, the fluid within the middle ear was collected every 2 hours, and the quantity of albumin in the fluid, an index of vascular leakage, was determined using enzyme‐linked immunosorbent assay.
L‐NAME significantly reduced LPS‐induced vascular extravasation into the middle ear. Inoculation of the ear with L‐arginine, the substrate for NO synthase, reversed the effects of L‐NAME. These results indicate that NO is a mediator of LPS‐induced MEE. Therefore, inhibition of NO synthase may represent a novel approach to the treatment of otitis media with effusion.</description><subject>Albumins - analysis</subject><subject>Animals</subject><subject>Arginine - administration & dosage</subject><subject>Arginine - analogs & derivatives</subject><subject>Biological and medical sciences</subject><subject>Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology</subject><subject>Enzyme Inhibitors - administration & dosage</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Lipopolysaccharides - administration & dosage</subject><subject>Medical sciences</subject><subject>NG-Nitroarginine Methyl Ester</subject><subject>Nitric Oxide - physiology</subject><subject>Nitric Oxide Synthase - antagonists & inhibitors</subject><subject>Non tumoral diseases</subject><subject>Otitis Media with Effusion - drug therapy</subject><subject>Otitis Media with Effusion - pathology</subject><subject>Otitis Media with Effusion - physiopathology</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Rats</subject><subject>Time Factors</subject><issn>0023-852X</issn><issn>1531-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkNtuEzEQhi0EKqHwCEi-QNyZ-nzgLoTQVkpbhEAcbiyvD5Jhs9vauyJ9e0wTcs1I1mhmvvnH-gGABL8h2Kgz3EIIphAxRmLdKtQepY_AgghGEDdGPAaL1mJIC_rtKXhW60-MiWICn4ATLY3Rhi_Au-s8lezhzS6H-BYu4VUM2U1jgWOC6yGM07jLA7ocwuxjgFc5hD7CtStwndJc8zjU5-BJcn2NLw75FHz5sP68ukCbm_PL1XKDPJeKIpdo6qTBXgfWCcUVdSJgz7zjmsrEqQrYOMJ91NEbn6imootOxCQ6LkVip-D1Xve2jHdzrJPd5upj37shjnO1SlMsiMQN1HvQl7HWEpO9LXnryr0l2P61z_6zzx7tsw_2tdWXhxtzt43huHjwq81fHeaueten4gaf6xFjRBvMTMPe77HfuY_3_33ebpafvgvBCW7dh9-gvUyuU9wdZVz5ZaViStiv1-eWkh9Sm81Hu2J_AGW1l_8</recordid><startdate>199608</startdate><enddate>199608</enddate><creator>Ball, Steven S.</creator><creator>Prazma, Jiri</creator><creator>Dais, C.G. Dean</creator><creator>Rosbe, Kristina W.</creator><creator>Pillsbury, Harold C.</creator><general>John Wiley & Sons, Inc</general><general>Wiley-Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8BM</scope></search><sort><creationdate>199608</creationdate><title>Nitric Oxide: A Mediator of Endotoxin-Induced Middle Ear Effusions</title><author>Ball, Steven S. ; Prazma, Jiri ; Dais, C.G. Dean ; Rosbe, Kristina W. ; Pillsbury, Harold C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4672-af2fb690c8d3b57472a5d0c3ca4826f427d09a14ce8ec9cf2825bea5ef5b465f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Albumins - analysis</topic><topic>Animals</topic><topic>Arginine - administration & dosage</topic><topic>Arginine - analogs & derivatives</topic><topic>Biological and medical sciences</topic><topic>Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology</topic><topic>Enzyme Inhibitors - administration & dosage</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Lipopolysaccharides - administration & dosage</topic><topic>Medical sciences</topic><topic>NG-Nitroarginine Methyl Ester</topic><topic>Nitric Oxide - physiology</topic><topic>Nitric Oxide Synthase - antagonists & inhibitors</topic><topic>Non tumoral diseases</topic><topic>Otitis Media with Effusion - drug therapy</topic><topic>Otitis Media with Effusion - pathology</topic><topic>Otitis Media with Effusion - physiopathology</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Rats</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ball, Steven S.</creatorcontrib><creatorcontrib>Prazma, Jiri</creatorcontrib><creatorcontrib>Dais, C.G. Dean</creatorcontrib><creatorcontrib>Rosbe, Kristina W.</creatorcontrib><creatorcontrib>Pillsbury, Harold C.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>ComDisDome</collection><jtitle>The Laryngoscope</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ball, Steven S.</au><au>Prazma, Jiri</au><au>Dais, C.G. Dean</au><au>Rosbe, Kristina W.</au><au>Pillsbury, Harold C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nitric Oxide: A Mediator of Endotoxin-Induced Middle Ear Effusions</atitle><jtitle>The Laryngoscope</jtitle><addtitle>The Laryngoscope</addtitle><date>1996-08</date><risdate>1996</risdate><volume>106</volume><issue>8</issue><spage>1021</spage><epage>1027</epage><pages>1021-1027</pages><issn>0023-852X</issn><eissn>1531-4995</eissn><coden>LARYA8</coden><abstract>Using a rat model, the authors investigated the role of nitric oxide (NO) in endotoxin‐induced middle ear effusion (MEE). After the eustachian tube was obstructed, the middle ear was transtympanically injected with 35μL of either 1 mg/mL lipopolysaccharide (LPS) or LPS and 1 mmol/L N‐nitro‐L‐arginine methyl ester (L‐NAME), a competitive inhibitor of NO synthase. Over the next 6 hours, the fluid within the middle ear was collected every 2 hours, and the quantity of albumin in the fluid, an index of vascular leakage, was determined using enzyme‐linked immunosorbent assay.
L‐NAME significantly reduced LPS‐induced vascular extravasation into the middle ear. Inoculation of the ear with L‐arginine, the substrate for NO synthase, reversed the effects of L‐NAME. These results indicate that NO is a mediator of LPS‐induced MEE. Therefore, inhibition of NO synthase may represent a novel approach to the treatment of otitis media with effusion.</abstract><cop>Hoboken, NJ</cop><pub>John Wiley & Sons, Inc</pub><pmid>8699894</pmid><doi>10.1097/00005537-199608000-00022</doi><tpages>7</tpages></addata></record> |
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| ispartof | The Laryngoscope, 1996-08, Vol.106 (8), p.1021-1027 |
| issn | 0023-852X 1531-4995 |
| language | eng |
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| source | MEDLINE; Journals@Ovid Ovid Autoload |
| subjects | Albumins - analysis Animals Arginine - administration & dosage Arginine - analogs & derivatives Biological and medical sciences Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology Enzyme Inhibitors - administration & dosage Enzyme-Linked Immunosorbent Assay Lipopolysaccharides - administration & dosage Medical sciences NG-Nitroarginine Methyl Ester Nitric Oxide - physiology Nitric Oxide Synthase - antagonists & inhibitors Non tumoral diseases Otitis Media with Effusion - drug therapy Otitis Media with Effusion - pathology Otitis Media with Effusion - physiopathology Otorhinolaryngology. Stomatology Rats Time Factors |
| title | Nitric Oxide: A Mediator of Endotoxin-Induced Middle Ear Effusions |
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