O-specific polysaccharide chain of Campylobacter fetus serotype B lipopolysaccharide is a D-rhamnan terminated with 3-O-methyl-D-rhamnose (D-acofriose)
An O-specific polysaccharide was liberated from Campylobacter fetus subsp. fetus serotype B lipopolysaccharide by mild acid hydrolysis followed by gel chromatography. This polysaccharide was found to contain D-rhamnose and 3-O-methyl-D-rhamnose (D-Rha3Me, D-acofriose) in a ratio of approximately 24:...
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| Veröffentlicht in: | European journal of biochemistry 1996-07, Vol.239 (2), p.434-438 |
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| container_title | European journal of biochemistry |
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| creator | Senchenkova, S.N Shashkov, A.S Knirel, Y.A McGovern, J.J Moran, A.P |
| description | An O-specific polysaccharide was liberated from Campylobacter fetus subsp. fetus serotype B lipopolysaccharide by mild acid hydrolysis followed by gel chromatography. This polysaccharide was found to contain D-rhamnose and 3-O-methyl-D-rhamnose (D-Rha3Me, D-acofriose) in a ratio of approximately 24:1, as well as lipopolysaccharide core constituents. The structure of the polysaccharide was studied by 1H-NMR and 13C-NMR spectroscopy, which included two-dimensional COSY, rotating-frame NOE spectroscopy (ROESY), and computer-assisted analysis of the 13C-NMR spectrum. Methylation analysis using [2H3]methyl iodide and Smith degradation followed by GLC/MS of the derived acetylated oligosaccharide-alditols was used to determine the location of D-acofriose. The O-specific polysaccharide is linear, consists on average of 12 disaccharide repeating units, and is terminated by a residue of D-acofriose. The following structure of the D-rhamnan chain was established: (...) |
| doi_str_mv | 10.1111/j.1432-1033.1996.0434u.x |
| format | Article |
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This polysaccharide was found to contain D-rhamnose and 3-O-methyl-D-rhamnose (D-Rha3Me, D-acofriose) in a ratio of approximately 24:1, as well as lipopolysaccharide core constituents. The structure of the polysaccharide was studied by 1H-NMR and 13C-NMR spectroscopy, which included two-dimensional COSY, rotating-frame NOE spectroscopy (ROESY), and computer-assisted analysis of the 13C-NMR spectrum. Methylation analysis using [2H3]methyl iodide and Smith degradation followed by GLC/MS of the derived acetylated oligosaccharide-alditols was used to determine the location of D-acofriose. The O-specific polysaccharide is linear, consists on average of 12 disaccharide repeating units, and is terminated by a residue of D-acofriose. The following structure of the D-rhamnan chain was established: (...)</description><identifier>ISSN: 0014-2956</identifier><identifier>EISSN: 1432-1033</identifier><identifier>DOI: 10.1111/j.1432-1033.1996.0434u.x</identifier><identifier>PMID: 8706751</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>3‐O‐methyl‐d‐rhamnose ; animal diseases ; animal health ; bacterial infections ; Campylobacter fetus ; Campylobacter fetus - chemistry ; Campylobacter fetus - immunology ; Carbohydrate Conformation ; Carbohydrate Sequence ; Chromatography, Gel ; Deoxy Sugars - analysis ; Deoxy Sugars - chemistry ; d‐rhamnose ; Gas Chromatography-Mass Spectrometry ; human health and safety ; Hydrocarbons, Iodinated ; lipopolysaccharide ; Lipopolysaccharides - chemistry ; Lipopolysaccharides - isolation & purification ; Magnetic Resonance Spectroscopy ; Mannans - analysis ; Mannans - chemistry ; Mannose - analogs & derivatives ; Mannose - analysis ; medicine ; Methylation ; Molecular Sequence Data ; O Antigens - chemistry ; O Antigens - isolation & purification ; O‐specific polysaccharide ; Software</subject><ispartof>European journal of biochemistry, 1996-07, Vol.239 (2), p.434-438</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c470U-f89d74be8ba8f782a29cf5a18ef968311f313448bb5cfd4212b7a419ff484dc93</citedby><cites>FETCH-LOGICAL-c470U-f89d74be8ba8f782a29cf5a18ef968311f313448bb5cfd4212b7a419ff484dc93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1432-1033.1996.0434u.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1432-1033.1996.0434u.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8706751$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Senchenkova, S.N</creatorcontrib><creatorcontrib>Shashkov, A.S</creatorcontrib><creatorcontrib>Knirel, Y.A</creatorcontrib><creatorcontrib>McGovern, J.J</creatorcontrib><creatorcontrib>Moran, A.P</creatorcontrib><title>O-specific polysaccharide chain of Campylobacter fetus serotype B lipopolysaccharide is a D-rhamnan terminated with 3-O-methyl-D-rhamnose (D-acofriose)</title><title>European journal of biochemistry</title><addtitle>Eur J Biochem</addtitle><description>An O-specific polysaccharide was liberated from Campylobacter fetus subsp. fetus serotype B lipopolysaccharide by mild acid hydrolysis followed by gel chromatography. This polysaccharide was found to contain D-rhamnose and 3-O-methyl-D-rhamnose (D-Rha3Me, D-acofriose) in a ratio of approximately 24:1, as well as lipopolysaccharide core constituents. The structure of the polysaccharide was studied by 1H-NMR and 13C-NMR spectroscopy, which included two-dimensional COSY, rotating-frame NOE spectroscopy (ROESY), and computer-assisted analysis of the 13C-NMR spectrum. Methylation analysis using [2H3]methyl iodide and Smith degradation followed by GLC/MS of the derived acetylated oligosaccharide-alditols was used to determine the location of D-acofriose. The O-specific polysaccharide is linear, consists on average of 12 disaccharide repeating units, and is terminated by a residue of D-acofriose. The following structure of the D-rhamnan chain was established: (...)</description><subject>3‐O‐methyl‐d‐rhamnose</subject><subject>animal diseases</subject><subject>animal health</subject><subject>bacterial infections</subject><subject>Campylobacter fetus</subject><subject>Campylobacter fetus - chemistry</subject><subject>Campylobacter fetus - immunology</subject><subject>Carbohydrate Conformation</subject><subject>Carbohydrate Sequence</subject><subject>Chromatography, Gel</subject><subject>Deoxy Sugars - analysis</subject><subject>Deoxy Sugars - chemistry</subject><subject>d‐rhamnose</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>human health and safety</subject><subject>Hydrocarbons, Iodinated</subject><subject>lipopolysaccharide</subject><subject>Lipopolysaccharides - chemistry</subject><subject>Lipopolysaccharides - isolation & purification</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Mannans - analysis</subject><subject>Mannans - chemistry</subject><subject>Mannose - analogs & derivatives</subject><subject>Mannose - analysis</subject><subject>medicine</subject><subject>Methylation</subject><subject>Molecular Sequence Data</subject><subject>O Antigens - chemistry</subject><subject>O Antigens - isolation & purification</subject><subject>O‐specific polysaccharide</subject><subject>Software</subject><issn>0014-2956</issn><issn>1432-1033</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUha2KqkwLj1DhFYKFg_-SOBukdtpCpUqzKLO2HMdmPEriYCdq8yS8Lk5n1AUb8ObaOud8V_IBABKckXS-7DPCGUUEM5aRqioyzBmfsucTsHoV3oAVxoQjWuXFW3Ae4x5jXFRFeQbORImLMicr8HuD4mC0s07DwbdzVFrvVHCNgWm6HnoL16ob5tbXSo8mQGvGKcJogh_nwcBr2LrB_xV1ESp4g8JOdb3qYYp1rlejaeCTG3eQoQ3qzLibW3Q0-WjgpxuktLfBpcfnd-DUqjaa98d5AbZ3tz_W39HD5tv9-uoBaV7iLbKiakpeG1ErYUtBFa20zRURxlaFYIRYRhjnoq5zbRtOCa1LxUllLRe80RW7AB8P3CH4X5OJo-xc1KZtVW_8FGVipq-l4p9GkpciL4qFKA5GHXyMwVg5BNepMEuC5VKe3MulI7l0JJfy5Et58jlFL487prozzWvw2FbSvx70J9ea-b-58u72-jFdtwnw4QCwykv1M7got48UE4ZJzijDgv0BE9K0XA</recordid><startdate>19960715</startdate><enddate>19960715</enddate><creator>Senchenkova, S.N</creator><creator>Shashkov, A.S</creator><creator>Knirel, Y.A</creator><creator>McGovern, J.J</creator><creator>Moran, A.P</creator><general>Blackwell Science Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19960715</creationdate><title>O-specific polysaccharide chain of Campylobacter fetus serotype B lipopolysaccharide is a D-rhamnan terminated with 3-O-methyl-D-rhamnose (D-acofriose)</title><author>Senchenkova, S.N ; Shashkov, A.S ; Knirel, Y.A ; McGovern, J.J ; Moran, A.P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c470U-f89d74be8ba8f782a29cf5a18ef968311f313448bb5cfd4212b7a419ff484dc93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>3‐O‐methyl‐d‐rhamnose</topic><topic>animal diseases</topic><topic>animal health</topic><topic>bacterial infections</topic><topic>Campylobacter fetus</topic><topic>Campylobacter fetus - chemistry</topic><topic>Campylobacter fetus - immunology</topic><topic>Carbohydrate Conformation</topic><topic>Carbohydrate Sequence</topic><topic>Chromatography, Gel</topic><topic>Deoxy Sugars - analysis</topic><topic>Deoxy Sugars - chemistry</topic><topic>d‐rhamnose</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>human health and safety</topic><topic>Hydrocarbons, Iodinated</topic><topic>lipopolysaccharide</topic><topic>Lipopolysaccharides - chemistry</topic><topic>Lipopolysaccharides - isolation & purification</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Mannans - analysis</topic><topic>Mannans - chemistry</topic><topic>Mannose - analogs & derivatives</topic><topic>Mannose - analysis</topic><topic>medicine</topic><topic>Methylation</topic><topic>Molecular Sequence Data</topic><topic>O Antigens - chemistry</topic><topic>O Antigens - isolation & purification</topic><topic>O‐specific polysaccharide</topic><topic>Software</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Senchenkova, S.N</creatorcontrib><creatorcontrib>Shashkov, A.S</creatorcontrib><creatorcontrib>Knirel, Y.A</creatorcontrib><creatorcontrib>McGovern, J.J</creatorcontrib><creatorcontrib>Moran, A.P</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Senchenkova, S.N</au><au>Shashkov, A.S</au><au>Knirel, Y.A</au><au>McGovern, J.J</au><au>Moran, A.P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>O-specific polysaccharide chain of Campylobacter fetus serotype B lipopolysaccharide is a D-rhamnan terminated with 3-O-methyl-D-rhamnose (D-acofriose)</atitle><jtitle>European journal of biochemistry</jtitle><addtitle>Eur J Biochem</addtitle><date>1996-07-15</date><risdate>1996</risdate><volume>239</volume><issue>2</issue><spage>434</spage><epage>438</epage><pages>434-438</pages><issn>0014-2956</issn><eissn>1432-1033</eissn><abstract>An O-specific polysaccharide was liberated from Campylobacter fetus subsp. fetus serotype B lipopolysaccharide by mild acid hydrolysis followed by gel chromatography. This polysaccharide was found to contain D-rhamnose and 3-O-methyl-D-rhamnose (D-Rha3Me, D-acofriose) in a ratio of approximately 24:1, as well as lipopolysaccharide core constituents. The structure of the polysaccharide was studied by 1H-NMR and 13C-NMR spectroscopy, which included two-dimensional COSY, rotating-frame NOE spectroscopy (ROESY), and computer-assisted analysis of the 13C-NMR spectrum. Methylation analysis using [2H3]methyl iodide and Smith degradation followed by GLC/MS of the derived acetylated oligosaccharide-alditols was used to determine the location of D-acofriose. The O-specific polysaccharide is linear, consists on average of 12 disaccharide repeating units, and is terminated by a residue of D-acofriose. The following structure of the D-rhamnan chain was established: (...)</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>8706751</pmid><doi>10.1111/j.1432-1033.1996.0434u.x</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | European journal of biochemistry, 1996-07, Vol.239 (2), p.434-438 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection |
| subjects | 3‐O‐methyl‐d‐rhamnose animal diseases animal health bacterial infections Campylobacter fetus Campylobacter fetus - chemistry Campylobacter fetus - immunology Carbohydrate Conformation Carbohydrate Sequence Chromatography, Gel Deoxy Sugars - analysis Deoxy Sugars - chemistry d‐rhamnose Gas Chromatography-Mass Spectrometry human health and safety Hydrocarbons, Iodinated lipopolysaccharide Lipopolysaccharides - chemistry Lipopolysaccharides - isolation & purification Magnetic Resonance Spectroscopy Mannans - analysis Mannans - chemistry Mannose - analogs & derivatives Mannose - analysis medicine Methylation Molecular Sequence Data O Antigens - chemistry O Antigens - isolation & purification O‐specific polysaccharide Software |
| title | O-specific polysaccharide chain of Campylobacter fetus serotype B lipopolysaccharide is a D-rhamnan terminated with 3-O-methyl-D-rhamnose (D-acofriose) |
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