Strand‐specific transcription of the simple sequences poly[(dG‐dT):(dC‐dA)] and poly[(dG‐dA):(dC‐dT)] in D. melanogaster embryos revealed by S1 nuclease treatment

The transcription of different strands of the simple sequences poly[(dG‐dT):(dC‐dA)] and poly[(dG‐dA):(dT‐dC)] in Drosophila melanogaster embryos was studied. After selective labelling of one strand, the polymers were hybridized with immobilized RNA and hybrids were treated on filters with S1 nuclea...

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Veröffentlicht in:	FEBS letters 1988-05, Vol.232 (1), p.235-237
Hauptverfasser:	Vashakidze, R.P., Mamulashvili, N.A., Kalandarishvili, K.G., Kolchinsky, A.M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Collodion DNA Transposable Elements Drosophila melanogaster Drosophila melanogaster - embryology Drosophila melanogaster - genetics Endonucleases - metabolism Nuclease S1 Nucleic Acid Hybridization Polydeoxyribonucleotides - genetics Polydeoxyribonucleotides - metabolism RNA - genetics Simple nucleotide sequence Single-Strand Specific DNA and RNA Endonucleases Transcription Transcription, Genetic
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creator	Vashakidze, R.P. Mamulashvili, N.A. Kalandarishvili, K.G. Kolchinsky, A.M.
description	The transcription of different strands of the simple sequences poly[(dG‐dT):(dC‐dA)] and poly[(dG‐dA):(dT‐dC)] in Drosophila melanogaster embryos was studied. After selective labelling of one strand, the polymers were hybridized with immobilized RNA and hybrids were treated on filters with S1 nuclease. Appropriate control has shown that this treatment destroys sandwich‐like complexes and leaves only true hybrids. Transcription of poly[(dG‐dA):(dT‐dC)] proved to be nearly symmetric, while poly(dG‐dT) is transcribed 3‐times more intensively than poly(dA‐dC). Our approach allowed us to estimate the relative content of simple sequences in RNA.
doi_str_mv	10.1016/0014-5793(88)80423-X
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source	MEDLINE; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection
subjects	Animals Collodion DNA Transposable Elements Drosophila melanogaster Drosophila melanogaster - embryology Drosophila melanogaster - genetics Endonucleases - metabolism Nuclease S1 Nucleic Acid Hybridization Polydeoxyribonucleotides - genetics Polydeoxyribonucleotides - metabolism RNA - genetics Simple nucleotide sequence Single-Strand Specific DNA and RNA Endonucleases Transcription Transcription, Genetic
title	Strand‐specific transcription of the simple sequences poly[(dG‐dT):(dC‐dA)] and poly[(dG‐dA):(dC‐dT)] in D. melanogaster embryos revealed by S1 nuclease treatment
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