Characterization of an Arabidopsis thaliana cDNA encoding an S-adenosylmethionine-sensitive threonine synthase Threonine synthase from higher plants

An Arabidopsis thaliana cDNA encoding an Sadenosylmethionine-sensitive threonine synthase (EC 4.2.99.2) has been isolated by functional complementation of an Escherichia coli mutant devoid of threonine synthase activity. Threonine synthase from A. thaliana was shown to be synthesized with a transit...

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Veröffentlicht in:	FEBS letters 1996-07, Vol.390 (1), p.85-90
Hauptverfasser:	Curien, Gilles, Dumas, Renaud, Ravanel, Stéphane, Douce, Roland
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Arabidopsis - enzymology Arabidopsis - genetics Arabidopsis thaliana Base Sequence Carbon-Oxygen Lyases Chloroplast Chromatography, Gel Chromatography, Ion Exchange Cloning, Molecular Conserved Sequence DNA Primers DNA, Complementary DTT, dithiothreitol Escherichia coli formula omitted IPTG, isopropyl β-d-thiogalactopyranoside Kinetics Lyases - biosynthesis Lyases - isolation & purification Lyases - metabolism Lysine Molecular Sequence Data o-Phospho-l-homoserine OPA, o-phthaldialdehyde OPH, o-phospho-l-homoserine PLP, pyridoxal 5′-phosphate Polymerase Chain Reaction Pyridoxal 5′-phosphate Recombinant Proteins - biosynthesis Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism S-adenosylmethionine S-Adenosylmethionine - pharmacology SAM, S-adenosylmethionine Sequence Homology, Amino Acid Threonine synthase
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