A fluorometric assay for peptidyl α-amidation activity using high-performance liquid chromatography

A rapid and sensitive method for the determination of peptidyl α-amidation activity has been developed and is based on reverse-phase high-performance liquid chromatographic separation and fluorometric detection. A dansylated tripeptide, N-dansyl-Tyr-Val-Gly-OH, is used as the substrate in the assay...

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Veröffentlicht in:	Analytical biochemistry 1988-02, Vol.168 (2), p.272-279
Hauptverfasser:	Jones, Barry N., Tamburini, Paul P., Consalvo, Angelo P., Young, Stanley D., Lovato, Susan J., Gilligan, James P., Jeng, Arco Y., Wennogle, Lawrence P.
Format:	Artikel
Sprache:	eng
Schlagworte:	amidation enzyme Amides - analysis Biological and medical sciences Chromatography, High Pressure Liquid Dansyl Compounds - analysis Diverse techniques fluorescent substrate fluorometry Fluorometry - methods Fundamental and applied biological sciences. Psychology high-performance liquid chromatography HPLC Molecular and cellular biology peptide hormones peptide processing peptide α-amidation Peptides - analysis
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