Hypoxia preferentially destroys GABAergic neurons in developing rat neocortex explants in culture
The hypothesis that hypoxic ischemia before or during the human birth process preferentially destroys GABAergic nerve cells, particularly in the neocortex, was tested in a tissue culture model system. To that end, rat neocortex explants dissected from 6-day-old rat pups and cultured to a development...
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| Veröffentlicht in: | Experimental neurology 1988-05, Vol.100 (2), p.332-340 |
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| container_end_page | 340 |
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| container_title | Experimental neurology |
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| creator | Romijn, H.J. Ruijter, J.M. Wolters, P.S. |
| description | The hypothesis that hypoxic ischemia before or during the human birth process preferentially destroys GABAergic nerve cells, particularly in the neocortex, was tested in a tissue culture model system. To that end, rat neocortex explants dissected from 6-day-old rat pups and cultured to a developmental stage approximately comparable to that of the newborn human neocortex were exposed to hypoxia for different periods. Quantitative light microscopic and immunocytochemical evaluation of the cultures demonstrated that GABAergic neurons were indeed the first neurons to die during hypoxia. |
| doi_str_mv | 10.1016/0014-4886(88)90112-4 |
| format | Article |
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To that end, rat neocortex explants dissected from 6-day-old rat pups and cultured to a developmental stage approximately comparable to that of the newborn human neocortex were exposed to hypoxia for different periods. 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To that end, rat neocortex explants dissected from 6-day-old rat pups and cultured to a developmental stage approximately comparable to that of the newborn human neocortex were exposed to hypoxia for different periods. Quantitative light microscopic and immunocytochemical evaluation of the cultures demonstrated that GABAergic neurons were indeed the first neurons to die during hypoxia.</description><subject>Animals</subject><subject>Cell Survival</subject><subject>Cells, Cultured</subject><subject>Cerebral Cortex - pathology</subject><subject>gamma-Aminobutyric Acid</subject><subject>Hypoxia, Brain - pathology</subject><subject>Neurons - pathology</subject><subject>Rats</subject><issn>0014-4886</issn><issn>1090-2430</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkbFu2zAQhomiges6fYMW0FS0g5I7kRGppYBjNEmBAFmSmWDIk8FCFlVSMuy3Dx0bGdOJw__d8cd3jH1FuEDA-hIARSmUqn8o9bMBxKoUH9gcoYGyEhw-svkb8ol9TukvADSikjM247wGkNWcmbv9EHbeFEOkliL1ozddty8cpTGGfSpul9dLimtvi56mGPpU-D6nW-rC4Pt1Ec2Yk2BDHGlX0G7oTD--QnbqxinSOTtrTZfoy-ldsKeb34-ru_L-4fbPanlfWoFyLB02XGJDxBGUdHRVW-5aYdxz665qKRxHYbhTreBcVariqIQAaaoKjcKm5gv2_bh3iOHflOvrjU-WutyHwpS0VKhAcflfEEVuckAXTBxBG0NK2Y8eot-YuNcI-nACffCrD361Uvr1BFrksW-n_dPzhtzb0Ml5zn8dc8o2tp6iTtZTb8n5SHbULvj3P3gBq-SVqg</recordid><startdate>19880501</startdate><enddate>19880501</enddate><creator>Romijn, H.J.</creator><creator>Ruijter, J.M.</creator><creator>Wolters, P.S.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19880501</creationdate><title>Hypoxia preferentially destroys GABAergic neurons in developing rat neocortex explants in culture</title><author>Romijn, H.J. ; Ruijter, J.M. ; Wolters, P.S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-d193719ee31087de56c3df4adbfd5674d314a3d8f43382823184407a221a81963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Animals</topic><topic>Cell Survival</topic><topic>Cells, Cultured</topic><topic>Cerebral Cortex - pathology</topic><topic>gamma-Aminobutyric Acid</topic><topic>Hypoxia, Brain - pathology</topic><topic>Neurons - pathology</topic><topic>Rats</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Romijn, H.J.</creatorcontrib><creatorcontrib>Ruijter, J.M.</creatorcontrib><creatorcontrib>Wolters, P.S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental neurology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Romijn, H.J.</au><au>Ruijter, J.M.</au><au>Wolters, P.S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hypoxia preferentially destroys GABAergic neurons in developing rat neocortex explants in culture</atitle><jtitle>Experimental neurology</jtitle><addtitle>Exp Neurol</addtitle><date>1988-05-01</date><risdate>1988</risdate><volume>100</volume><issue>2</issue><spage>332</spage><epage>340</epage><pages>332-340</pages><issn>0014-4886</issn><eissn>1090-2430</eissn><abstract>The hypothesis that hypoxic ischemia before or during the human birth process preferentially destroys GABAergic nerve cells, particularly in the neocortex, was tested in a tissue culture model system. 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| ispartof | Experimental neurology, 1988-05, Vol.100 (2), p.332-340 |
| issn | 0014-4886 1090-2430 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Cell Survival Cells, Cultured Cerebral Cortex - pathology gamma-Aminobutyric Acid Hypoxia, Brain - pathology Neurons - pathology Rats |
| title | Hypoxia preferentially destroys GABAergic neurons in developing rat neocortex explants in culture |
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