Characterization of a Chromosome-Specific Chimpanzee Alpha Satellite Subset: Evolutionary Relationship to Subsets on Human Chromosomes
Alpha satellite DNA is a tandemly repeated DNA family found at the centromeres of all primate chromosomes examined. The fundamental repeat units of alpha satellite DNA are diverged 169- to 172-bp monomers, often found to be organized in chromosome-specific higher-order repeat units. The chromosomes...
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| Veröffentlicht in: | Genomics 1996-04, Vol.33 (2), p.220-228 |
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| creator | Warburton, Peter E. Haaf, Thomas Gosden, John Lawson, Diane Willard, Huntington F. |
| description | Alpha satellite DNA is a tandemly repeated DNA family found at the centromeres of all primate chromosomes examined. The fundamental repeat units of alpha satellite DNA are diverged 169- to 172-bp monomers, often found to be organized in chromosome-specific higher-order repeat units. The chromosomes of human (Homo sapiens(HSA)), chimpanzee (Pan troglodytes(PTR) andPan paniscus), and gorilla (Gorilla gorilla) share a remarkable similarity and synteny. It is of interest to ask if alpha satellite arrays at centromeres of homologous chromosomes between these species are closely related (evolving in an orthologous manner) or if the evolutionary processes that homogenize and spread these arrays within and between chromosomes result in nonorthologous evolution of arrays. By using PCR primers specific for human chromosome 17-specific alpha satellite DNA, we have amplified, cloned, and characterized a chromosome-specific subset from the PTR chimpanzee genome. Hybridization both on Southern blots andin situas well as sequence analysis show that this subset is most closely related, as expected, to sequences on HSA 17. However,in situhybridization reveals that this subset is not found on the homologous chromosome in chimpanzee (PTR 19), but instead on PTR 12, which is homologous to HSA 2p. |
| doi_str_mv | 10.1006/geno.1996.0187 |
| format | Article |
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The fundamental repeat units of alpha satellite DNA are diverged 169- to 172-bp monomers, often found to be organized in chromosome-specific higher-order repeat units. The chromosomes of human (Homo sapiens(HSA)), chimpanzee (Pan troglodytes(PTR) andPan paniscus), and gorilla (Gorilla gorilla) share a remarkable similarity and synteny. It is of interest to ask if alpha satellite arrays at centromeres of homologous chromosomes between these species are closely related (evolving in an orthologous manner) or if the evolutionary processes that homogenize and spread these arrays within and between chromosomes result in nonorthologous evolution of arrays. By using PCR primers specific for human chromosome 17-specific alpha satellite DNA, we have amplified, cloned, and characterized a chromosome-specific subset from the PTR chimpanzee genome. Hybridization both on Southern blots andin situas well as sequence analysis show that this subset is most closely related, as expected, to sequences on HSA 17. However,in situhybridization reveals that this subset is not found on the homologous chromosome in chimpanzee (PTR 19), but instead on PTR 12, which is homologous to HSA 2p.</description><identifier>ISSN: 0888-7543</identifier><identifier>EISSN: 1089-8646</identifier><identifier>DOI: 10.1006/geno.1996.0187</identifier><identifier>PMID: 8660971</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Animals ; Base Sequence ; Biological and medical sciences ; BIOLOGICAL EVOLUTION ; BIOLOGY AND MEDICINE, BASIC STUDIES ; Cell Line ; CENTROMERES ; Chromosomes ; Classical genetics, quantitative genetics, hybrids ; COMPARATIVE EVALUATIONS ; DNA ; DNA HYBRIDIZATION ; DNA SEQUENCING ; DNA, Satellite ; DNA-CLONING ; ELECTROPHORESIS ; Electrophoresis, Gel, Pulsed-Field ; Fundamental and applied biological sciences. Psychology ; GENETIC MAPPING ; Genetics of eukaryotes. Biological and molecular evolution ; Gorilla gorilla ; Homo sapiens ; HUMAN CHROMOSOMES ; Humans ; IN-SITU HYBRIDIZATION ; Molecular Sequence Data ; Pan paniscus ; Pan troglodytes ; Pan troglodytes - genetics ; POLYMERASE CHAIN REACTION ; PRIMATES ; Vertebrata</subject><ispartof>Genomics, 1996-04, Vol.33 (2), p.220-228</ispartof><rights>1996 Academic Press</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c425t-6db2e2589a2f490968704aff80cc302ae2c92a5fcde45fa5a3af21229a0bf3683</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0888754396901871$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3062010$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8660971$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/465952$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Warburton, Peter E.</creatorcontrib><creatorcontrib>Haaf, Thomas</creatorcontrib><creatorcontrib>Gosden, John</creatorcontrib><creatorcontrib>Lawson, Diane</creatorcontrib><creatorcontrib>Willard, Huntington F.</creatorcontrib><title>Characterization of a Chromosome-Specific Chimpanzee Alpha Satellite Subset: Evolutionary Relationship to Subsets on Human Chromosomes</title><title>Genomics</title><addtitle>Genomics</addtitle><description>Alpha satellite DNA is a tandemly repeated DNA family found at the centromeres of all primate chromosomes examined. The fundamental repeat units of alpha satellite DNA are diverged 169- to 172-bp monomers, often found to be organized in chromosome-specific higher-order repeat units. The chromosomes of human (Homo sapiens(HSA)), chimpanzee (Pan troglodytes(PTR) andPan paniscus), and gorilla (Gorilla gorilla) share a remarkable similarity and synteny. It is of interest to ask if alpha satellite arrays at centromeres of homologous chromosomes between these species are closely related (evolving in an orthologous manner) or if the evolutionary processes that homogenize and spread these arrays within and between chromosomes result in nonorthologous evolution of arrays. By using PCR primers specific for human chromosome 17-specific alpha satellite DNA, we have amplified, cloned, and characterized a chromosome-specific subset from the PTR chimpanzee genome. Hybridization both on Southern blots andin situas well as sequence analysis show that this subset is most closely related, as expected, to sequences on HSA 17. However,in situhybridization reveals that this subset is not found on the homologous chromosome in chimpanzee (PTR 19), but instead on PTR 12, which is homologous to HSA 2p.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>BIOLOGICAL EVOLUTION</subject><subject>BIOLOGY AND MEDICINE, BASIC STUDIES</subject><subject>Cell Line</subject><subject>CENTROMERES</subject><subject>Chromosomes</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>COMPARATIVE EVALUATIONS</subject><subject>DNA</subject><subject>DNA HYBRIDIZATION</subject><subject>DNA SEQUENCING</subject><subject>DNA, Satellite</subject><subject>DNA-CLONING</subject><subject>ELECTROPHORESIS</subject><subject>Electrophoresis, Gel, Pulsed-Field</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENETIC MAPPING</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Gorilla gorilla</subject><subject>Homo sapiens</subject><subject>HUMAN CHROMOSOMES</subject><subject>Humans</subject><subject>IN-SITU HYBRIDIZATION</subject><subject>Molecular Sequence Data</subject><subject>Pan paniscus</subject><subject>Pan troglodytes</subject><subject>Pan troglodytes - genetics</subject><subject>POLYMERASE CHAIN REACTION</subject><subject>PRIMATES</subject><subject>Vertebrata</subject><issn>0888-7543</issn><issn>1089-8646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhS0EKtvClRtSkBC3LLYTOza3alUoUiUkFs7WrDMmRkkc7KRS-wP43TjdVcUFcbLk-ebNvHmEvGJ0yyiV73_gGLZMa7mlTDVPyIZRpUsla_mUbKhSqmxEXT0n5yn9pJTqSvEzcqakpLphG_J710EEO2P09zD7MBbBFVDsuhiGkMKA5X5C6523-c8PE4z3iMVlP3VQ7GHGvvczFvvlkHD-UFzdhn5ZVSDeFV-xf1BMnZ-KOZygVOQZ18sA419D0gvyzEGf8OXpvSDfP159212XN18-fd5d3pS25mIuZXvgyIXSwF2tqZaqoTU4p6i1FeWA3GoOwtkWa-FAQAWOM8410IOrpKouyJujbkizN8nm5W1nwziinU0thRY8M--OzBTDrwXTbAafbHYKI4YlmUaxpqqY-i_IhOS8Fiu4PYI2hpQiOjNFP-QbGUbNmqJZUzRrimZNMTe8PikvhwHbR_wUW66_PdUhWehdhNH69IhVVHLKaMbUEcN80luPcXWMo8XWx9VwG_y_NvgDkWy6xA</recordid><startdate>19960415</startdate><enddate>19960415</enddate><creator>Warburton, Peter E.</creator><creator>Haaf, Thomas</creator><creator>Gosden, John</creator><creator>Lawson, Diane</creator><creator>Willard, Huntington F.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>19960415</creationdate><title>Characterization of a Chromosome-Specific Chimpanzee Alpha Satellite Subset: Evolutionary Relationship to Subsets on Human Chromosomes</title><author>Warburton, Peter E. ; Haaf, Thomas ; Gosden, John ; Lawson, Diane ; Willard, Huntington F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c425t-6db2e2589a2f490968704aff80cc302ae2c92a5fcde45fa5a3af21229a0bf3683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>BIOLOGICAL EVOLUTION</topic><topic>BIOLOGY AND MEDICINE, BASIC STUDIES</topic><topic>Cell Line</topic><topic>CENTROMERES</topic><topic>Chromosomes</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>COMPARATIVE EVALUATIONS</topic><topic>DNA</topic><topic>DNA HYBRIDIZATION</topic><topic>DNA SEQUENCING</topic><topic>DNA, Satellite</topic><topic>DNA-CLONING</topic><topic>ELECTROPHORESIS</topic><topic>Electrophoresis, Gel, Pulsed-Field</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENETIC MAPPING</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Gorilla gorilla</topic><topic>Homo sapiens</topic><topic>HUMAN CHROMOSOMES</topic><topic>Humans</topic><topic>IN-SITU HYBRIDIZATION</topic><topic>Molecular Sequence Data</topic><topic>Pan paniscus</topic><topic>Pan troglodytes</topic><topic>Pan troglodytes - genetics</topic><topic>POLYMERASE CHAIN REACTION</topic><topic>PRIMATES</topic><topic>Vertebrata</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Warburton, Peter E.</creatorcontrib><creatorcontrib>Haaf, Thomas</creatorcontrib><creatorcontrib>Gosden, John</creatorcontrib><creatorcontrib>Lawson, Diane</creatorcontrib><creatorcontrib>Willard, Huntington F.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Genomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Warburton, Peter E.</au><au>Haaf, Thomas</au><au>Gosden, John</au><au>Lawson, Diane</au><au>Willard, Huntington F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of a Chromosome-Specific Chimpanzee Alpha Satellite Subset: Evolutionary Relationship to Subsets on Human Chromosomes</atitle><jtitle>Genomics</jtitle><addtitle>Genomics</addtitle><date>1996-04-15</date><risdate>1996</risdate><volume>33</volume><issue>2</issue><spage>220</spage><epage>228</epage><pages>220-228</pages><issn>0888-7543</issn><eissn>1089-8646</eissn><abstract>Alpha satellite DNA is a tandemly repeated DNA family found at the centromeres of all primate chromosomes examined. The fundamental repeat units of alpha satellite DNA are diverged 169- to 172-bp monomers, often found to be organized in chromosome-specific higher-order repeat units. The chromosomes of human (Homo sapiens(HSA)), chimpanzee (Pan troglodytes(PTR) andPan paniscus), and gorilla (Gorilla gorilla) share a remarkable similarity and synteny. It is of interest to ask if alpha satellite arrays at centromeres of homologous chromosomes between these species are closely related (evolving in an orthologous manner) or if the evolutionary processes that homogenize and spread these arrays within and between chromosomes result in nonorthologous evolution of arrays. By using PCR primers specific for human chromosome 17-specific alpha satellite DNA, we have amplified, cloned, and characterized a chromosome-specific subset from the PTR chimpanzee genome. Hybridization both on Southern blots andin situas well as sequence analysis show that this subset is most closely related, as expected, to sequences on HSA 17. However,in situhybridization reveals that this subset is not found on the homologous chromosome in chimpanzee (PTR 19), but instead on PTR 12, which is homologous to HSA 2p.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>8660971</pmid><doi>10.1006/geno.1996.0187</doi><tpages>9</tpages></addata></record> |
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| ispartof | Genomics, 1996-04, Vol.33 (2), p.220-228 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Base Sequence Biological and medical sciences BIOLOGICAL EVOLUTION BIOLOGY AND MEDICINE, BASIC STUDIES Cell Line CENTROMERES Chromosomes Classical genetics, quantitative genetics, hybrids COMPARATIVE EVALUATIONS DNA DNA HYBRIDIZATION DNA SEQUENCING DNA, Satellite DNA-CLONING ELECTROPHORESIS Electrophoresis, Gel, Pulsed-Field Fundamental and applied biological sciences. Psychology GENETIC MAPPING Genetics of eukaryotes. Biological and molecular evolution Gorilla gorilla Homo sapiens HUMAN CHROMOSOMES Humans IN-SITU HYBRIDIZATION Molecular Sequence Data Pan paniscus Pan troglodytes Pan troglodytes - genetics POLYMERASE CHAIN REACTION PRIMATES Vertebrata |
| title | Characterization of a Chromosome-Specific Chimpanzee Alpha Satellite Subset: Evolutionary Relationship to Subsets on Human Chromosomes |
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