Synthesis, purification, and characterization of the cytoplasmic domain of the human insulin receptor using a baculovirus expression system

The cytoplasmic domain of the beta subunit of the human insulin receptor has been overexpressed in insect cells using the baculovirus expression system. A recombinant baculovirus (BIR-2) was constructed by inserting the human insulin proreceptor cDNA fragment that encodes the cytoplasmic domain of t...

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Veröffentlicht in:	The Journal of biological chemistry 1988-04, Vol.263 (12), p.5560-5568
Hauptverfasser:	Herrera, R, Lebwohl, D, Garcia de Herreros, A, Kallen, R G, Rosen, O M
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Chromatography Cytoplasm - metabolism DNA, Recombinant - metabolism Electrophoresis, Polyacrylamide Gel Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Humans Immunosorbent Techniques Insect Viruses - genetics Insect Viruses - metabolism Macromolecular Substances Manganese - pharmacology Methods. Procedures. Technologies Molecular cloning Molecular Weight Peptide Fragments Phosphorylation Phosphotyrosine Protein-Tyrosine Kinases - genetics Protein-Tyrosine Kinases - isolation & purification Protein-Tyrosine Kinases - metabolism Receptor, Insulin - genetics Receptor, Insulin - metabolism Transfection Trypsin Tyrosine - analogs & derivatives Tyrosine - metabolism
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