Detection of Pneumocystis carinii sequences in serum by polymerase chain reaction
The clinical significance of the detection of Pneumocystis carinii DNA was evaluated, as well as the detection of circulating P. carinii antigen from serum using previously collected samples. Fourteen serum samples from 13 patients were diagnosed positively for P. carinii DNA by polymerase chain rea...
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| Veröffentlicht in: | Respiratory medicine 1996-03, Vol.90 (3), p.153-157 |
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| container_title | Respiratory medicine |
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| creator | Miyawaki, H. Fujita, J. Hojo, S. Harada, M. Yamaji, Y. Suguri, S. Takahara, J. |
| description | The clinical significance of the detection of
Pneumocystis carinii DNA was evaluated, as well as the detection of circulating
P. carinii antigen from serum using previously collected samples. Fourteen serum samples from 13 patients were diagnosed positively for
P. carinii DNA by polymerase chain reaction (PCR). Ten of 14 episodes (71·4%) of pulmonary complications were compatible with
P. carinii pneumonia. Two patients were definitely diagnosed as having had
P. carinii pneumonia at autopsy. All patients positive for circulating antigens were also positive for
P. carinii DNA, suggesting that the detection of
P. carinii DNA by PCR is more sensitive compared to the detection of circulating antigens by the Ouchterlony method. It is concluded that the detection of
P. carinii DNA in serum by PCR provides useful information for identifying
P. carinii pneumonia. |
| doi_str_mv | 10.1016/S0954-6111(96)90157-2 |
| format | Article |
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Pneumocystis carinii DNA was evaluated, as well as the detection of circulating
P. carinii antigen from serum using previously collected samples. Fourteen serum samples from 13 patients were diagnosed positively for
P. carinii DNA by polymerase chain reaction (PCR). Ten of 14 episodes (71·4%) of pulmonary complications were compatible with
P. carinii pneumonia. Two patients were definitely diagnosed as having had
P. carinii pneumonia at autopsy. All patients positive for circulating antigens were also positive for
P. carinii DNA, suggesting that the detection of
P. carinii DNA by PCR is more sensitive compared to the detection of circulating antigens by the Ouchterlony method. It is concluded that the detection of
P. carinii DNA in serum by PCR provides useful information for identifying
P. carinii pneumonia.</description><identifier>ISSN: 0954-6111</identifier><identifier>EISSN: 1532-3064</identifier><identifier>DOI: 10.1016/S0954-6111(96)90157-2</identifier><identifier>PMID: 8736207</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Amibiasis ; Antigens, Fungal - blood ; Base Sequence ; Biological and medical sciences ; DNA Primers - genetics ; DNA, Fungal - blood ; Female ; Human mycoses ; Human protozoal diseases ; Humans ; Infectious diseases ; Male ; Medical sciences ; Middle Aged ; Miscellaneous ; Molecular Sequence Data ; Mycoses ; Mycoses of the respiratory system ; Parasitic diseases ; Pneumocystis - genetics ; Pneumocystis - immunology ; Pneumonia, Pneumocystis - diagnosis ; Polymerase Chain Reaction ; Predictive Value of Tests ; Protozoal diseases</subject><ispartof>Respiratory medicine, 1996-03, Vol.90 (3), p.153-157</ispartof><rights>1996</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-f9dc9a9b3316f3a4f5f1608f382064df81feeeda839aed49345f11adfdb7f5ef3</citedby><cites>FETCH-LOGICAL-c436t-f9dc9a9b3316f3a4f5f1608f382064df81feeeda839aed49345f11adfdb7f5ef3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0954-6111(96)90157-2$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3050473$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8736207$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miyawaki, H.</creatorcontrib><creatorcontrib>Fujita, J.</creatorcontrib><creatorcontrib>Hojo, S.</creatorcontrib><creatorcontrib>Harada, M.</creatorcontrib><creatorcontrib>Yamaji, Y.</creatorcontrib><creatorcontrib>Suguri, S.</creatorcontrib><creatorcontrib>Takahara, J.</creatorcontrib><title>Detection of Pneumocystis carinii sequences in serum by polymerase chain reaction</title><title>Respiratory medicine</title><addtitle>Respir Med</addtitle><description>The clinical significance of the detection of
Pneumocystis carinii DNA was evaluated, as well as the detection of circulating
P. carinii antigen from serum using previously collected samples. Fourteen serum samples from 13 patients were diagnosed positively for
P. carinii DNA by polymerase chain reaction (PCR). Ten of 14 episodes (71·4%) of pulmonary complications were compatible with
P. carinii pneumonia. Two patients were definitely diagnosed as having had
P. carinii pneumonia at autopsy. All patients positive for circulating antigens were also positive for
P. carinii DNA, suggesting that the detection of
P. carinii DNA by PCR is more sensitive compared to the detection of circulating antigens by the Ouchterlony method. It is concluded that the detection of
P. carinii DNA in serum by PCR provides useful information for identifying
P. carinii pneumonia.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Amibiasis</subject><subject>Antigens, Fungal - blood</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>DNA Primers - genetics</subject><subject>DNA, Fungal - blood</subject><subject>Female</subject><subject>Human mycoses</subject><subject>Human protozoal diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Miscellaneous</subject><subject>Molecular Sequence Data</subject><subject>Mycoses</subject><subject>Mycoses of the respiratory system</subject><subject>Parasitic diseases</subject><subject>Pneumocystis - genetics</subject><subject>Pneumocystis - immunology</subject><subject>Pneumonia, Pneumocystis - diagnosis</subject><subject>Polymerase Chain Reaction</subject><subject>Predictive Value of Tests</subject><subject>Protozoal diseases</subject><issn>0954-6111</issn><issn>1532-3064</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkF1LwzAUhoMoc05_gtALEb2oJk2aNlci8xMGKup1SJMTjPRjJq2wf2-2ld16FcL7nHNeHoROCb4imPDrdyxylnJCyIXglwKTvEizPTQlOc1SijnbR9MdcoiOQvjGGAvG8ARNyoLyDBdT9HYHPejedW3S2eS1haHp9Cr0LiRaedc6lwT4GaDVEBLXxo8fmqRaJcuuXjXgVYBEf6mYeFCbPcfowKo6wMn4ztDnw_3H_CldvDw-z28XqWaU96kVRgslKkoJt1Qxm1vCcWlpmcXuxpbEAoBRJRUKDBOURYAoY01V2BwsnaHz7d6l72LB0MvGBQ11rVrohiCLknBWZDSC-RbUvgvBg5VL7xrlV5JguVYpNyrl2pMUXG5UyizOnY4HhqoBs5sa3cX8bMxV0Kq2XrXahR1GcY5ZsT5_s8Ugyvh14GXQbu3TOB_NS9O5f4r8AYcoka4</recordid><startdate>19960301</startdate><enddate>19960301</enddate><creator>Miyawaki, H.</creator><creator>Fujita, J.</creator><creator>Hojo, S.</creator><creator>Harada, M.</creator><creator>Yamaji, Y.</creator><creator>Suguri, S.</creator><creator>Takahara, J.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19960301</creationdate><title>Detection of Pneumocystis carinii sequences in serum by polymerase chain reaction</title><author>Miyawaki, H. ; 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Pneumocystis carinii DNA was evaluated, as well as the detection of circulating
P. carinii antigen from serum using previously collected samples. Fourteen serum samples from 13 patients were diagnosed positively for
P. carinii DNA by polymerase chain reaction (PCR). Ten of 14 episodes (71·4%) of pulmonary complications were compatible with
P. carinii pneumonia. Two patients were definitely diagnosed as having had
P. carinii pneumonia at autopsy. All patients positive for circulating antigens were also positive for
P. carinii DNA, suggesting that the detection of
P. carinii DNA by PCR is more sensitive compared to the detection of circulating antigens by the Ouchterlony method. It is concluded that the detection of
P. carinii DNA in serum by PCR provides useful information for identifying
P. carinii pneumonia.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>8736207</pmid><doi>10.1016/S0954-6111(96)90157-2</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Respiratory medicine, 1996-03, Vol.90 (3), p.153-157 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals |
| subjects | Adult Aged Aged, 80 and over Amibiasis Antigens, Fungal - blood Base Sequence Biological and medical sciences DNA Primers - genetics DNA, Fungal - blood Female Human mycoses Human protozoal diseases Humans Infectious diseases Male Medical sciences Middle Aged Miscellaneous Molecular Sequence Data Mycoses Mycoses of the respiratory system Parasitic diseases Pneumocystis - genetics Pneumocystis - immunology Pneumonia, Pneumocystis - diagnosis Polymerase Chain Reaction Predictive Value of Tests Protozoal diseases |
| title | Detection of Pneumocystis carinii sequences in serum by polymerase chain reaction |
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