Differential and developmental expression of beta-tubulins in a higher plant
By using two-dimensional gel electrophoresis and immunoblotting, we have analyzed the expression of beta-tubulin isotypes in the higher plant, carrot. We report a complex expression of beta-tubulins that is dependent on the developmental stage of the tissues analyzed. Consequently, each tissue exami...
Gespeichert in:
| Veröffentlicht in: | The Journal of biological chemistry 1988-04, Vol.263 (11), p.5474-5479 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 5479 |
|---|---|
| container_issue | 11 |
| container_start_page | 5474 |
| container_title | The Journal of biological chemistry |
| container_volume | 263 |
| creator | Hussey, P J Lloyd, C W Gull, K |
| description | By using two-dimensional gel electrophoresis and immunoblotting, we have analyzed the expression of beta-tubulin isotypes in the higher plant, carrot. We report a complex expression of beta-tubulins that is dependent on the developmental stage of the tissues analyzed. Consequently, each tissue examined can be identified by its unique composition of beta-tubulins. In total, there are six electrophoretically separable beta-tubulins. In no tissue, however, is there less than two or more than five beta-tubulins. Within this framework we have detected a beta-tubulin specific to seedling tissue beta 6, and a beta-tubulin, beta 5, that is found only in the vegetative tissues of the mature plant. Traced from stem to midrib to leaf lamina, the beta 5 isotype becomes progressively dominant relative to beta 1. Another beta-tubulin isotype, beta 4, appears in marked abundance in immature and mature stamens. In isolated mature pollen the beta 4-tubulin overwhelmingly predominates the ubiquitously expressed beta 2-tubulin isotype. The remaining beta-tubulin isotypes also have specific expression programs with beta 1 present in all tissues except pollen and beta 3 absent only from pollen and leafy tissues. |
| doi_str_mv | 10.1016/S0021-9258(18)60741-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_78164350</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0021925818607410</els_id><sourcerecordid>78164350</sourcerecordid><originalsourceid>FETCH-LOGICAL-c483t-1ee659cbfac4b15d34b8a7d515d80cfdf226b1a8595cc67967e353f4cca4b1803</originalsourceid><addsrcrecordid>eNqFkE2L1TAUhoMo43X0DwgDBUV0Uc1pPrsSGT_hgotxwF1I05O5kX5N0o76702nl7s1m4RznvMmeQi5APoWKMh3V5RWUNaV0K9Bv5FUcSjpA7IDqlnJBPx8SHYn5DF5ktIvmhev4YycsUpDzasd2X8M3mPEYQ62K-zQFi3eYTdOfS7lCv6ZIqYUxqEYfdHgbMt5aZYuDKkIQ2GLQ7g5YCymzg7zU_LI2y7hs-N-Tq4_f_px-bXcf__y7fLDvnRcs7kERClq13jreAOiZbzRVrUiHzV1vvVVJRuwWtTCOalqqZAJ5rlzNvOasnPyasud4ni7YJpNH5LDLr8BxyUZpUFyJlZQbKCLY0oRvZli6G38a4Ca1aK5t2hWRQa0ubdo1rmL4wVL02N7mjpqy_2Xx75NznY-2sGFdMKUpkrCGvNiw1ZJv0NE04TRHbA3lWQGwAiueKaeb5S3o7E3MQddX2nNVMXq3Hy_NTH7vAsYTXIBB4dtjnOzacfwn7_8AyOsomM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>78164350</pqid></control><display><type>article</type><title>Differential and developmental expression of beta-tubulins in a higher plant</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Hussey, P J ; Lloyd, C W ; Gull, K</creator><creatorcontrib>Hussey, P J ; Lloyd, C W ; Gull, K</creatorcontrib><description>By using two-dimensional gel electrophoresis and immunoblotting, we have analyzed the expression of beta-tubulin isotypes in the higher plant, carrot. We report a complex expression of beta-tubulins that is dependent on the developmental stage of the tissues analyzed. Consequently, each tissue examined can be identified by its unique composition of beta-tubulins. In total, there are six electrophoretically separable beta-tubulins. In no tissue, however, is there less than two or more than five beta-tubulins. Within this framework we have detected a beta-tubulin specific to seedling tissue beta 6, and a beta-tubulin, beta 5, that is found only in the vegetative tissues of the mature plant. Traced from stem to midrib to leaf lamina, the beta 5 isotype becomes progressively dominant relative to beta 1. Another beta-tubulin isotype, beta 4, appears in marked abundance in immature and mature stamens. In isolated mature pollen the beta 4-tubulin overwhelmingly predominates the ubiquitously expressed beta 2-tubulin isotype. The remaining beta-tubulin isotypes also have specific expression programs with beta 1 present in all tissues except pollen and beta 3 absent only from pollen and leafy tissues.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(18)60741-0</identifier><identifier>PMID: 3281942</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: Elsevier Inc</publisher><subject>Biological and medical sciences ; CELL WALLS ; DAUCUS CAROTA ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Immunosorbent Techniques ; MEIOSE ; MEIOSIS ; Molecular and cellular biology ; Molecular genetics ; MORFOGENESIS ; MORPHOGENESE ; MORPHOGENESIS ; PARED CELULAR ; PAROI CELLULAIRE ; Plants - analysis ; POLEN ; POLLEN ; Tubulin - biosynthesis</subject><ispartof>The Journal of biological chemistry, 1988-04, Vol.263 (11), p.5474-5479</ispartof><rights>1988 © 1988 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.</rights><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c483t-1ee659cbfac4b15d34b8a7d515d80cfdf226b1a8595cc67967e353f4cca4b1803</citedby><cites>FETCH-LOGICAL-c483t-1ee659cbfac4b15d34b8a7d515d80cfdf226b1a8595cc67967e353f4cca4b1803</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7807610$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3281942$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hussey, P J</creatorcontrib><creatorcontrib>Lloyd, C W</creatorcontrib><creatorcontrib>Gull, K</creatorcontrib><title>Differential and developmental expression of beta-tubulins in a higher plant</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>By using two-dimensional gel electrophoresis and immunoblotting, we have analyzed the expression of beta-tubulin isotypes in the higher plant, carrot. We report a complex expression of beta-tubulins that is dependent on the developmental stage of the tissues analyzed. Consequently, each tissue examined can be identified by its unique composition of beta-tubulins. In total, there are six electrophoretically separable beta-tubulins. In no tissue, however, is there less than two or more than five beta-tubulins. Within this framework we have detected a beta-tubulin specific to seedling tissue beta 6, and a beta-tubulin, beta 5, that is found only in the vegetative tissues of the mature plant. Traced from stem to midrib to leaf lamina, the beta 5 isotype becomes progressively dominant relative to beta 1. Another beta-tubulin isotype, beta 4, appears in marked abundance in immature and mature stamens. In isolated mature pollen the beta 4-tubulin overwhelmingly predominates the ubiquitously expressed beta 2-tubulin isotype. The remaining beta-tubulin isotypes also have specific expression programs with beta 1 present in all tissues except pollen and beta 3 absent only from pollen and leafy tissues.</description><subject>Biological and medical sciences</subject><subject>CELL WALLS</subject><subject>DAUCUS CAROTA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Immunosorbent Techniques</subject><subject>MEIOSE</subject><subject>MEIOSIS</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>MORFOGENESIS</subject><subject>MORPHOGENESE</subject><subject>MORPHOGENESIS</subject><subject>PARED CELULAR</subject><subject>PAROI CELLULAIRE</subject><subject>Plants - analysis</subject><subject>POLEN</subject><subject>POLLEN</subject><subject>Tubulin - biosynthesis</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE2L1TAUhoMo43X0DwgDBUV0Uc1pPrsSGT_hgotxwF1I05O5kX5N0o76702nl7s1m4RznvMmeQi5APoWKMh3V5RWUNaV0K9Bv5FUcSjpA7IDqlnJBPx8SHYn5DF5ktIvmhev4YycsUpDzasd2X8M3mPEYQ62K-zQFi3eYTdOfS7lCv6ZIqYUxqEYfdHgbMt5aZYuDKkIQ2GLQ7g5YCymzg7zU_LI2y7hs-N-Tq4_f_px-bXcf__y7fLDvnRcs7kERClq13jreAOiZbzRVrUiHzV1vvVVJRuwWtTCOalqqZAJ5rlzNvOasnPyasud4ni7YJpNH5LDLr8BxyUZpUFyJlZQbKCLY0oRvZli6G38a4Ca1aK5t2hWRQa0ubdo1rmL4wVL02N7mjpqy_2Xx75NznY-2sGFdMKUpkrCGvNiw1ZJv0NE04TRHbA3lWQGwAiueKaeb5S3o7E3MQddX2nNVMXq3Hy_NTH7vAsYTXIBB4dtjnOzacfwn7_8AyOsomM</recordid><startdate>19880415</startdate><enddate>19880415</enddate><creator>Hussey, P J</creator><creator>Lloyd, C W</creator><creator>Gull, K</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19880415</creationdate><title>Differential and developmental expression of beta-tubulins in a higher plant</title><author>Hussey, P J ; Lloyd, C W ; Gull, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c483t-1ee659cbfac4b15d34b8a7d515d80cfdf226b1a8595cc67967e353f4cca4b1803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Biological and medical sciences</topic><topic>CELL WALLS</topic><topic>DAUCUS CAROTA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Immunosorbent Techniques</topic><topic>MEIOSE</topic><topic>MEIOSIS</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>MORFOGENESIS</topic><topic>MORPHOGENESE</topic><topic>MORPHOGENESIS</topic><topic>PARED CELULAR</topic><topic>PAROI CELLULAIRE</topic><topic>Plants - analysis</topic><topic>POLEN</topic><topic>POLLEN</topic><topic>Tubulin - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hussey, P J</creatorcontrib><creatorcontrib>Lloyd, C W</creatorcontrib><creatorcontrib>Gull, K</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hussey, P J</au><au>Lloyd, C W</au><au>Gull, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential and developmental expression of beta-tubulins in a higher plant</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1988-04-15</date><risdate>1988</risdate><volume>263</volume><issue>11</issue><spage>5474</spage><epage>5479</epage><pages>5474-5479</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>By using two-dimensional gel electrophoresis and immunoblotting, we have analyzed the expression of beta-tubulin isotypes in the higher plant, carrot. We report a complex expression of beta-tubulins that is dependent on the developmental stage of the tissues analyzed. Consequently, each tissue examined can be identified by its unique composition of beta-tubulins. In total, there are six electrophoretically separable beta-tubulins. In no tissue, however, is there less than two or more than five beta-tubulins. Within this framework we have detected a beta-tubulin specific to seedling tissue beta 6, and a beta-tubulin, beta 5, that is found only in the vegetative tissues of the mature plant. Traced from stem to midrib to leaf lamina, the beta 5 isotype becomes progressively dominant relative to beta 1. Another beta-tubulin isotype, beta 4, appears in marked abundance in immature and mature stamens. In isolated mature pollen the beta 4-tubulin overwhelmingly predominates the ubiquitously expressed beta 2-tubulin isotype. The remaining beta-tubulin isotypes also have specific expression programs with beta 1 present in all tissues except pollen and beta 3 absent only from pollen and leafy tissues.</abstract><cop>Bethesda, MD</cop><pub>Elsevier Inc</pub><pmid>3281942</pmid><doi>10.1016/S0021-9258(18)60741-0</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9258 |
| ispartof | The Journal of biological chemistry, 1988-04, Vol.263 (11), p.5474-5479 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78164350 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Biological and medical sciences CELL WALLS DAUCUS CAROTA Fundamental and applied biological sciences. Psychology Gene expression Immunosorbent Techniques MEIOSE MEIOSIS Molecular and cellular biology Molecular genetics MORFOGENESIS MORPHOGENESE MORPHOGENESIS PARED CELULAR PAROI CELLULAIRE Plants - analysis POLEN POLLEN Tubulin - biosynthesis |
| title | Differential and developmental expression of beta-tubulins in a higher plant |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T00%3A46%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Differential%20and%20developmental%20expression%20of%20beta-tubulins%20in%20a%20higher%20plant&rft.jtitle=The%20Journal%20of%20biological%20chemistry&rft.au=Hussey,%20P%20J&rft.date=1988-04-15&rft.volume=263&rft.issue=11&rft.spage=5474&rft.epage=5479&rft.pages=5474-5479&rft.issn=0021-9258&rft.eissn=1083-351X&rft.coden=JBCHA3&rft_id=info:doi/10.1016/S0021-9258(18)60741-0&rft_dat=%3Cproquest_cross%3E78164350%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=78164350&rft_id=info:pmid/3281942&rft_els_id=S0021925818607410&rfr_iscdi=true |