Determination of transforming growth factor activity in effusions from cancer patients
Transforming growth factors (TGF) are polypeptides that stimulate anchorage‐independent growth of various nontransformed cells in vitro. Transforming growth factors have been found in tumor extracts and in the urine of cancer patients. The specific questions of our study were whether TGF activity ca...
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| Veröffentlicht in: | Cancer 1988-05, Vol.61 (9), p.1832-1837 |
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| container_title | Cancer |
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| creator | Hanauske, Axel‐R. Arteaga, Carlos L. Clark, Gary M. Buchok, Joy Marshall, Martha Hazarika, Parul Pardue, Robert L. Von Hoff, Daniel D. |
| description | Transforming growth factors (TGF) are polypeptides that stimulate anchorage‐independent growth of various nontransformed cells in vitro. Transforming growth factors have been found in tumor extracts and in the urine of cancer patients. The specific questions of our study were whether TGF activity can be detected in malignant effusions, how different assays for TGF correlate with each other, and how assays for TGF correlate with soft agar tumor colony formation as measured by the human tumor cloning assay (HTCA). The TGF activity was measured by a normal rat kidney transformation assay (NRKA), a 125I‐EGF radioreceptor assay (RRA), and a radioimmunoassay (RIA) for TGF‐α. Cells from effusions were cytologically examined and plated in the HTCA. A total of 104 effusions from cancer patients and 17 effusions from non‐cancer patients was tested. Transforming growth factor activity was detected in some specimens. Only the TGF‐α RIA and the HTCA showed significant differences between cancer and noncancer patients. Immunoreactive TGF‐α was measurable in some cases by RIA even when cytologic testing failed to detect malignant cells. Spearman correlations between assays indicated that RIA results correlate significantly with all other assays. It is concluded that TGF‐α activity might be important for in vitro colony formation of human tumor cells. |
| doi_str_mv | 10.1002/1097-0142(19880501)61:9<1832::AID-CNCR2820610919>3.0.CO;2-J |
| format | Article |
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Transforming growth factors have been found in tumor extracts and in the urine of cancer patients. The specific questions of our study were whether TGF activity can be detected in malignant effusions, how different assays for TGF correlate with each other, and how assays for TGF correlate with soft agar tumor colony formation as measured by the human tumor cloning assay (HTCA). The TGF activity was measured by a normal rat kidney transformation assay (NRKA), a 125I‐EGF radioreceptor assay (RRA), and a radioimmunoassay (RIA) for TGF‐α. Cells from effusions were cytologically examined and plated in the HTCA. A total of 104 effusions from cancer patients and 17 effusions from non‐cancer patients was tested. Transforming growth factor activity was detected in some specimens. Only the TGF‐α RIA and the HTCA showed significant differences between cancer and noncancer patients. Immunoreactive TGF‐α was measurable in some cases by RIA even when cytologic testing failed to detect malignant cells. Spearman correlations between assays indicated that RIA results correlate significantly with all other assays. It is concluded that TGF‐α activity might be important for in vitro colony formation of human tumor cells.</description><identifier>ISSN: 0008-543X</identifier><identifier>EISSN: 1097-0142</identifier><identifier>DOI: 10.1002/1097-0142(19880501)61:9<1832::AID-CNCR2820610919>3.0.CO;2-J</identifier><identifier>PMID: 3162700</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Ascitic Fluid - analysis ; Ascitic Fluid - pathology ; Breast Neoplasms - metabolism ; Breast Neoplasms - pathology ; ErbB Receptors - analysis ; Female ; Humans ; Lung Neoplasms - metabolism ; Lung Neoplasms - pathology ; Neoplasms - metabolism ; Neoplasms - pathology ; Ovarian Neoplasms - metabolism ; Ovarian Neoplasms - pathology ; Peptides - analysis ; Pleural Effusion - metabolism ; Pleural Effusion - pathology ; Transforming Growth Factors ; Tumor Stem Cell Assay</subject><ispartof>Cancer, 1988-05, Vol.61 (9), p.1832-1837</ispartof><rights>Copyright © 1988 American Cancer Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c4699-b461d3d103bec2ed5cebda70fe91c29f9094a12cb63695a9bded1d8628de97d43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3162700$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hanauske, Axel‐R.</creatorcontrib><creatorcontrib>Arteaga, Carlos L.</creatorcontrib><creatorcontrib>Clark, Gary M.</creatorcontrib><creatorcontrib>Buchok, Joy</creatorcontrib><creatorcontrib>Marshall, Martha</creatorcontrib><creatorcontrib>Hazarika, Parul</creatorcontrib><creatorcontrib>Pardue, Robert L.</creatorcontrib><creatorcontrib>Von Hoff, Daniel D.</creatorcontrib><title>Determination of transforming growth factor activity in effusions from cancer patients</title><title>Cancer</title><addtitle>Cancer</addtitle><description>Transforming growth factors (TGF) are polypeptides that stimulate anchorage‐independent growth of various nontransformed cells in vitro. Transforming growth factors have been found in tumor extracts and in the urine of cancer patients. The specific questions of our study were whether TGF activity can be detected in malignant effusions, how different assays for TGF correlate with each other, and how assays for TGF correlate with soft agar tumor colony formation as measured by the human tumor cloning assay (HTCA). The TGF activity was measured by a normal rat kidney transformation assay (NRKA), a 125I‐EGF radioreceptor assay (RRA), and a radioimmunoassay (RIA) for TGF‐α. Cells from effusions were cytologically examined and plated in the HTCA. A total of 104 effusions from cancer patients and 17 effusions from non‐cancer patients was tested. Transforming growth factor activity was detected in some specimens. Only the TGF‐α RIA and the HTCA showed significant differences between cancer and noncancer patients. Immunoreactive TGF‐α was measurable in some cases by RIA even when cytologic testing failed to detect malignant cells. Spearman correlations between assays indicated that RIA results correlate significantly with all other assays. It is concluded that TGF‐α activity might be important for in vitro colony formation of human tumor cells.</description><subject>Ascitic Fluid - analysis</subject><subject>Ascitic Fluid - pathology</subject><subject>Breast Neoplasms - metabolism</subject><subject>Breast Neoplasms - pathology</subject><subject>ErbB Receptors - analysis</subject><subject>Female</subject><subject>Humans</subject><subject>Lung Neoplasms - metabolism</subject><subject>Lung Neoplasms - pathology</subject><subject>Neoplasms - metabolism</subject><subject>Neoplasms - pathology</subject><subject>Ovarian Neoplasms - metabolism</subject><subject>Ovarian Neoplasms - pathology</subject><subject>Peptides - analysis</subject><subject>Pleural Effusion - metabolism</subject><subject>Pleural Effusion - pathology</subject><subject>Transforming Growth Factors</subject><subject>Tumor Stem Cell Assay</subject><issn>0008-543X</issn><issn>1097-0142</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkFuL1EAQhRtR1nH1Jwj9JPqQsao76aRHEZasl10WB0RF8KHo9GWNTJKxO-My_96EGRf0QfCli65z6lTxMVYjLBFAPEfQZQaYi6eoqwoKwGcKV_olVlKsVmcX51n9vv4gKgFqsqJ-JZewrNcvRHZ5hy1up--yBQBUWZHLL_fZg5S-T99SFPKEnUhUogRYsM_nfvSxa3sztkPPh8DHaPoUhrl3za_jcDN-48HYcYh8etuf7bjnbc99CLs0jSQe4tBxa3rrI99OMb4f00N2L5hN8o-O9ZR9evP6Y_0uu1q_vajPrjKbK62zJlfopEOQjbfCu8L6xpkSgtdohQ4adG5Q2EZJpQujG-cdukqJynldulyesieH3G0cfux8Gqlrk_Wbjen9sEtUVqikQDEZvx6MNg4pRR9oG9vOxD0h0EydZm40c6Pf1EkhaZqpE03U6U_qJAmoXpOgyyn98fGMXdN5d5t9xDzp4aDftBu__7_V_9z8lyJ_AbuLoYk</recordid><startdate>19880501</startdate><enddate>19880501</enddate><creator>Hanauske, Axel‐R.</creator><creator>Arteaga, Carlos L.</creator><creator>Clark, Gary M.</creator><creator>Buchok, Joy</creator><creator>Marshall, Martha</creator><creator>Hazarika, Parul</creator><creator>Pardue, Robert L.</creator><creator>Von Hoff, Daniel D.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19880501</creationdate><title>Determination of transforming growth factor activity in effusions from cancer patients</title><author>Hanauske, Axel‐R. ; Arteaga, Carlos L. ; Clark, Gary M. ; Buchok, Joy ; Marshall, Martha ; Hazarika, Parul ; Pardue, Robert L. ; Von Hoff, Daniel D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4699-b461d3d103bec2ed5cebda70fe91c29f9094a12cb63695a9bded1d8628de97d43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Ascitic Fluid - analysis</topic><topic>Ascitic Fluid - pathology</topic><topic>Breast Neoplasms - metabolism</topic><topic>Breast Neoplasms - pathology</topic><topic>ErbB Receptors - analysis</topic><topic>Female</topic><topic>Humans</topic><topic>Lung Neoplasms - metabolism</topic><topic>Lung Neoplasms - pathology</topic><topic>Neoplasms - metabolism</topic><topic>Neoplasms - pathology</topic><topic>Ovarian Neoplasms - metabolism</topic><topic>Ovarian Neoplasms - pathology</topic><topic>Peptides - analysis</topic><topic>Pleural Effusion - metabolism</topic><topic>Pleural Effusion - pathology</topic><topic>Transforming Growth Factors</topic><topic>Tumor Stem Cell Assay</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hanauske, Axel‐R.</creatorcontrib><creatorcontrib>Arteaga, Carlos L.</creatorcontrib><creatorcontrib>Clark, Gary M.</creatorcontrib><creatorcontrib>Buchok, Joy</creatorcontrib><creatorcontrib>Marshall, Martha</creatorcontrib><creatorcontrib>Hazarika, Parul</creatorcontrib><creatorcontrib>Pardue, Robert L.</creatorcontrib><creatorcontrib>Von Hoff, Daniel D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hanauske, Axel‐R.</au><au>Arteaga, Carlos L.</au><au>Clark, Gary M.</au><au>Buchok, Joy</au><au>Marshall, Martha</au><au>Hazarika, Parul</au><au>Pardue, Robert L.</au><au>Von Hoff, Daniel D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of transforming growth factor activity in effusions from cancer patients</atitle><jtitle>Cancer</jtitle><addtitle>Cancer</addtitle><date>1988-05-01</date><risdate>1988</risdate><volume>61</volume><issue>9</issue><spage>1832</spage><epage>1837</epage><pages>1832-1837</pages><issn>0008-543X</issn><eissn>1097-0142</eissn><abstract>Transforming growth factors (TGF) are polypeptides that stimulate anchorage‐independent growth of various nontransformed cells in vitro. Transforming growth factors have been found in tumor extracts and in the urine of cancer patients. The specific questions of our study were whether TGF activity can be detected in malignant effusions, how different assays for TGF correlate with each other, and how assays for TGF correlate with soft agar tumor colony formation as measured by the human tumor cloning assay (HTCA). The TGF activity was measured by a normal rat kidney transformation assay (NRKA), a 125I‐EGF radioreceptor assay (RRA), and a radioimmunoassay (RIA) for TGF‐α. Cells from effusions were cytologically examined and plated in the HTCA. A total of 104 effusions from cancer patients and 17 effusions from non‐cancer patients was tested. Transforming growth factor activity was detected in some specimens. Only the TGF‐α RIA and the HTCA showed significant differences between cancer and noncancer patients. Immunoreactive TGF‐α was measurable in some cases by RIA even when cytologic testing failed to detect malignant cells. Spearman correlations between assays indicated that RIA results correlate significantly with all other assays. It is concluded that TGF‐α activity might be important for in vitro colony formation of human tumor cells.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>3162700</pmid><doi>10.1002/1097-0142(19880501)61:9<1832::AID-CNCR2820610919>3.0.CO;2-J</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Cancer, 1988-05, Vol.61 (9), p.1832-1837 |
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| subjects | Ascitic Fluid - analysis Ascitic Fluid - pathology Breast Neoplasms - metabolism Breast Neoplasms - pathology ErbB Receptors - analysis Female Humans Lung Neoplasms - metabolism Lung Neoplasms - pathology Neoplasms - metabolism Neoplasms - pathology Ovarian Neoplasms - metabolism Ovarian Neoplasms - pathology Peptides - analysis Pleural Effusion - metabolism Pleural Effusion - pathology Transforming Growth Factors Tumor Stem Cell Assay |
| title | Determination of transforming growth factor activity in effusions from cancer patients |
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