Pituitary adenylate cyclase-activating polypeptide acts within the medial basal hypothalamus to inhibit prolactin and luteinizing hormone secretion
In this study, we investigated the hypothalamic regulatory role of pituitary adenylate cyclase-activating polypeptide (PACAP) in the control of LH and PRL secretion. Overiectomized ewes were surgically prepared with bilateral guide tubes directed at the preoptic area (POA) or medial basal hypothalam...
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| Veröffentlicht in: | Endocrinology (Philadelphia) 1996-08, Vol.137 (8), p.3424-3429 |
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| creator | Anderson, S T Sawangjaroen, K Curlewis, J D |
| description | In this study, we investigated the hypothalamic regulatory role of pituitary adenylate cyclase-activating polypeptide (PACAP) in the control of LH and PRL secretion. Overiectomized ewes were surgically prepared with bilateral guide tubes directed at the preoptic area (POA) or medial basal hypothalamus (MBH). After recovery from surgery, PACAP38 (0.1 nmol in 2.5 microliters over 1 h) or vehicle was bilaterally infused into each site in separate trials. Infusion of PACAP38 into the POA had no effect on either LH or PRL secretion. However, infusion of the peptide into the MBH suppressed PRL secretion during the 3-h postinfusion period; the responding animals (n = 9) had injectors located in the arcuate nucleus. In the three nonresponding animals, both injectors were outside the arcuate nucleus. Mean LH concentration, LH pulse frequency, and pulse amplitude were also significantly suppressed, with LH pulsatility declining in seven of eight animals during infusion of the peptide in the MBH. These results suggest that PACAP acts in the arcuate nucleus region of the MBH, and not the rostral POA, to inhibit both LH and PRL secretion. |
| doi_str_mv | 10.1210/en.137.8.3424 |
| format | Article |
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Overiectomized ewes were surgically prepared with bilateral guide tubes directed at the preoptic area (POA) or medial basal hypothalamus (MBH). After recovery from surgery, PACAP38 (0.1 nmol in 2.5 microliters over 1 h) or vehicle was bilaterally infused into each site in separate trials. Infusion of PACAP38 into the POA had no effect on either LH or PRL secretion. However, infusion of the peptide into the MBH suppressed PRL secretion during the 3-h postinfusion period; the responding animals (n = 9) had injectors located in the arcuate nucleus. In the three nonresponding animals, both injectors were outside the arcuate nucleus. Mean LH concentration, LH pulse frequency, and pulse amplitude were also significantly suppressed, with LH pulsatility declining in seven of eight animals during infusion of the peptide in the MBH. These results suggest that PACAP acts in the arcuate nucleus region of the MBH, and not the rostral POA, to inhibit both LH and PRL secretion.</description><identifier>ISSN: 0013-7227</identifier><identifier>EISSN: 1945-7170</identifier><identifier>DOI: 10.1210/en.137.8.3424</identifier><identifier>PMID: 8754770</identifier><language>eng</language><publisher>United States: Endocrine Society</publisher><subject>Animals ; Arcuate nucleus ; Arcuate Nucleus of Hypothalamus - physiology ; Female ; Guide tubes ; Hypothalamus ; Hypothalamus (medial) ; Hypothalamus, Middle - physiology ; Injectors ; Luteinizing hormone ; Luteinizing Hormone - antagonists & inhibitors ; Luteinizing Hormone - secretion ; Neuropeptides - pharmacology ; Ovariectomy ; Pituitary Adenylate Cyclase-Activating Polypeptide ; Polypeptides ; Preoptic area ; Preoptic area (medial) ; Preoptic Area - physiology ; Prolactin ; Prolactin - antagonists & inhibitors ; Prolactin - secretion ; Pulse amplitude ; Secretion ; Sheep</subject><ispartof>Endocrinology (Philadelphia), 1996-08, Vol.137 (8), p.3424-3429</ispartof><rights>Copyright © 1996 by The Endocrine Society 1996</rights><rights>Copyright © 1996 by The Endocrine Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c348t-5fccd7d9a598ebb75feffd38fb12f29b7d99fa243418191283575e5f395dcc773</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8754770$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Anderson, S T</creatorcontrib><creatorcontrib>Sawangjaroen, K</creatorcontrib><creatorcontrib>Curlewis, J D</creatorcontrib><title>Pituitary adenylate cyclase-activating polypeptide acts within the medial basal hypothalamus to inhibit prolactin and luteinizing hormone secretion</title><title>Endocrinology (Philadelphia)</title><addtitle>Endocrinology</addtitle><description>In this study, we investigated the hypothalamic regulatory role of pituitary adenylate cyclase-activating polypeptide (PACAP) in the control of LH and PRL secretion. Overiectomized ewes were surgically prepared with bilateral guide tubes directed at the preoptic area (POA) or medial basal hypothalamus (MBH). After recovery from surgery, PACAP38 (0.1 nmol in 2.5 microliters over 1 h) or vehicle was bilaterally infused into each site in separate trials. Infusion of PACAP38 into the POA had no effect on either LH or PRL secretion. However, infusion of the peptide into the MBH suppressed PRL secretion during the 3-h postinfusion period; the responding animals (n = 9) had injectors located in the arcuate nucleus. In the three nonresponding animals, both injectors were outside the arcuate nucleus. Mean LH concentration, LH pulse frequency, and pulse amplitude were also significantly suppressed, with LH pulsatility declining in seven of eight animals during infusion of the peptide in the MBH. These results suggest that PACAP acts in the arcuate nucleus region of the MBH, and not the rostral POA, to inhibit both LH and PRL secretion.</description><subject>Animals</subject><subject>Arcuate nucleus</subject><subject>Arcuate Nucleus of Hypothalamus - physiology</subject><subject>Female</subject><subject>Guide tubes</subject><subject>Hypothalamus</subject><subject>Hypothalamus (medial)</subject><subject>Hypothalamus, Middle - physiology</subject><subject>Injectors</subject><subject>Luteinizing hormone</subject><subject>Luteinizing Hormone - antagonists & inhibitors</subject><subject>Luteinizing Hormone - secretion</subject><subject>Neuropeptides - pharmacology</subject><subject>Ovariectomy</subject><subject>Pituitary Adenylate Cyclase-Activating Polypeptide</subject><subject>Polypeptides</subject><subject>Preoptic area</subject><subject>Preoptic area (medial)</subject><subject>Preoptic Area - physiology</subject><subject>Prolactin</subject><subject>Prolactin - antagonists & inhibitors</subject><subject>Prolactin - secretion</subject><subject>Pulse amplitude</subject><subject>Secretion</subject><subject>Sheep</subject><issn>0013-7227</issn><issn>1945-7170</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdUU2LFTEQDKKsz9WjRyEgLF7mmU-SHGXRVVjQg3sOmUyPkyWTjJOMMvs3_MPmsQ8PXrrprqIoqhB6TcmRMkreQzpSro76yAUTT9CBGiE7RRV5ig6EUN4pxtRz9KKU-3YKIfgFutBKCqXIAf35FuoWqlt37AZIe3QVsN99dAU652v45WpIP_CS477AUsMAuL0L_h3qFBKuE-AZhuAi7l1pc9qXXCcX3bwVXDMOaQp9qHhZczzpJezSgONWIaTwcJKe8jrnBLiAX6GGnF6iZ6OLBV6d9yW6-_Tx-_Xn7vbrzZfrD7ed50LXTo7eD2owThoNfa_kCOM4cD32lI3M9A0yo2OCC6qpoUxzqSTIkRs5eK8Uv0RXj7rN288NSrVzKB5idAnyVqzSVBpDdCO-_Y94n7c1NW-WU06E4ZSxxnpzZm19i8Qua5hbrvacdcPfPeJ5W_6BlNhTixaSbS1abU8t8r-fD5FL</recordid><startdate>19960801</startdate><enddate>19960801</enddate><creator>Anderson, S T</creator><creator>Sawangjaroen, K</creator><creator>Curlewis, J D</creator><general>Endocrine Society</general><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19960801</creationdate><title>Pituitary adenylate cyclase-activating polypeptide acts within the medial basal hypothalamus to inhibit prolactin and luteinizing hormone secretion</title><author>Anderson, S T ; Sawangjaroen, K ; Curlewis, J D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c348t-5fccd7d9a598ebb75feffd38fb12f29b7d99fa243418191283575e5f395dcc773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Arcuate nucleus</topic><topic>Arcuate Nucleus of Hypothalamus - physiology</topic><topic>Female</topic><topic>Guide tubes</topic><topic>Hypothalamus</topic><topic>Hypothalamus (medial)</topic><topic>Hypothalamus, Middle - physiology</topic><topic>Injectors</topic><topic>Luteinizing hormone</topic><topic>Luteinizing Hormone - antagonists & inhibitors</topic><topic>Luteinizing Hormone - secretion</topic><topic>Neuropeptides - pharmacology</topic><topic>Ovariectomy</topic><topic>Pituitary Adenylate Cyclase-Activating Polypeptide</topic><topic>Polypeptides</topic><topic>Preoptic area</topic><topic>Preoptic area (medial)</topic><topic>Preoptic Area - physiology</topic><topic>Prolactin</topic><topic>Prolactin - antagonists & inhibitors</topic><topic>Prolactin - secretion</topic><topic>Pulse amplitude</topic><topic>Secretion</topic><topic>Sheep</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Anderson, S T</creatorcontrib><creatorcontrib>Sawangjaroen, K</creatorcontrib><creatorcontrib>Curlewis, J D</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Endocrinology (Philadelphia)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Anderson, S T</au><au>Sawangjaroen, K</au><au>Curlewis, J D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pituitary adenylate cyclase-activating polypeptide acts within the medial basal hypothalamus to inhibit prolactin and luteinizing hormone secretion</atitle><jtitle>Endocrinology (Philadelphia)</jtitle><addtitle>Endocrinology</addtitle><date>1996-08-01</date><risdate>1996</risdate><volume>137</volume><issue>8</issue><spage>3424</spage><epage>3429</epage><pages>3424-3429</pages><issn>0013-7227</issn><eissn>1945-7170</eissn><abstract>In this study, we investigated the hypothalamic regulatory role of pituitary adenylate cyclase-activating polypeptide (PACAP) in the control of LH and PRL secretion. Overiectomized ewes were surgically prepared with bilateral guide tubes directed at the preoptic area (POA) or medial basal hypothalamus (MBH). After recovery from surgery, PACAP38 (0.1 nmol in 2.5 microliters over 1 h) or vehicle was bilaterally infused into each site in separate trials. Infusion of PACAP38 into the POA had no effect on either LH or PRL secretion. However, infusion of the peptide into the MBH suppressed PRL secretion during the 3-h postinfusion period; the responding animals (n = 9) had injectors located in the arcuate nucleus. In the three nonresponding animals, both injectors were outside the arcuate nucleus. Mean LH concentration, LH pulse frequency, and pulse amplitude were also significantly suppressed, with LH pulsatility declining in seven of eight animals during infusion of the peptide in the MBH. These results suggest that PACAP acts in the arcuate nucleus region of the MBH, and not the rostral POA, to inhibit both LH and PRL secretion.</abstract><cop>United States</cop><pub>Endocrine Society</pub><pmid>8754770</pmid><doi>10.1210/en.137.8.3424</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Animals Arcuate nucleus Arcuate Nucleus of Hypothalamus - physiology Female Guide tubes Hypothalamus Hypothalamus (medial) Hypothalamus, Middle - physiology Injectors Luteinizing hormone Luteinizing Hormone - antagonists & inhibitors Luteinizing Hormone - secretion Neuropeptides - pharmacology Ovariectomy Pituitary Adenylate Cyclase-Activating Polypeptide Polypeptides Preoptic area Preoptic area (medial) Preoptic Area - physiology Prolactin Prolactin - antagonists & inhibitors Prolactin - secretion Pulse amplitude Secretion Sheep |
| title | Pituitary adenylate cyclase-activating polypeptide acts within the medial basal hypothalamus to inhibit prolactin and luteinizing hormone secretion |
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