Two essential steps for a successful intracytoplasmic sperm injection: injection of immobilized spermatozoa after rupture of the oolema
A total of 740 cycles of intracytoplasmic sperm injection (ICSI) were performed: 625 cycles when
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| Veröffentlicht in: | Human reproduction (Oxford) 1996-03, Vol.11 (3), p.540-547 |
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| container_title | Human reproduction (Oxford) |
| container_volume | 11 |
| creator | Vanderzwalmen, P. Bertin, G. Lejeune, B. Nijs, M. Vandamme, B. Schoysman, R. |
| description | A total of 740 cycles of intracytoplasmic sperm injection (ICSI) were performed: 625 cycles when |
| doi_str_mv | 10.1093/HUMREP/11.3.540 |
| format | Article |
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An average of two pronuclei were observed in 63% of the injected oocytes, allowing 725 transfers of a maximum of three embryos (98%). Of 214 pregnancies initiated, 179 were established (25% of ICSI attempts). Because the fertilization rates from our initial 80 ICSI cycles were 2-fold less than those achieved previously, we changed the injection procedure and analysed, in 740 ICSI attempts, the importance of interfering technical factors and how to establish a successful ICSI programme, A remarkable change in the fertilization rate up to 68% (595 cycles) occurred when two steps in the injection procedure were performed well, i.e. immobilization of the spermatozoon and placement of the spermatozoon into the ooplasm after cytoplasmic aspiration into the pipette until oolema rupture. This immobilization, by touching the tail with the pipette, is mandatory for increasing the percentage of fertilization, even with totally non-motile spermatozoa (41%). Because aspiration of the cytoplasm is an invasive part of the ICSI procedure and influences the quality of the embryos, it is essential to reduce the amount of cytoplasm drawn into the pipette. This could be attained by using a spikeless injection pipette with the smallest possible internal diameter.</description><identifier>ISSN: 0268-1161</identifier><identifier>EISSN: 1460-2350</identifier><identifier>DOI: 10.1093/HUMREP/11.3.540</identifier><identifier>PMID: 8671262</identifier><identifier>CODEN: HUREEE</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Biological and medical sciences ; Birth control ; Cytoplasm ; cytoplasmic aspiration ; Embryo Transfer ; Embryonic and Fetal Development ; Female ; Fertilization in Vitro - methods ; Gynecology. Andrology. Obstetrics ; Humans ; ICSI ; immobilization of the spermatozoon ; immotile spermatozoa ; Male ; male factor infertility ; Medical sciences ; Microinjections - instrumentation ; Microinjections - methods ; Oocytes ; Pregnancy ; Sperm Motility ; Spermatozoa ; Sterility. Assisted procreation</subject><ispartof>Human reproduction (Oxford), 1996-03, Vol.11 (3), p.540-547</ispartof><rights>European Society for Human Reproduction and Embryology 1996</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c501t-32453de0058ff37856c355fbf1c41c4cb2b276a3c11e4c58ff1e4462e41c0d2c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1578,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3062723$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8671262$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vanderzwalmen, P.</creatorcontrib><creatorcontrib>Bertin, G.</creatorcontrib><creatorcontrib>Lejeune, B.</creatorcontrib><creatorcontrib>Nijs, M.</creatorcontrib><creatorcontrib>Vandamme, B.</creatorcontrib><creatorcontrib>Schoysman, R.</creatorcontrib><title>Two essential steps for a successful intracytoplasmic sperm injection: injection of immobilized spermatozoa after rupture of the oolema</title><title>Human reproduction (Oxford)</title><addtitle>Hum Reprod</addtitle><description>A total of 740 cycles of intracytoplasmic sperm injection (ICSI) were performed: 625 cycles when <6×105 total motile spermatozoa were harvestable from the ejaculate and 115 cycles in cases with a history of previous fertilization failure after classic in-vitro fertilization or subzonal sperm injection. An average of two pronuclei were observed in 63% of the injected oocytes, allowing 725 transfers of a maximum of three embryos (98%). Of 214 pregnancies initiated, 179 were established (25% of ICSI attempts). Because the fertilization rates from our initial 80 ICSI cycles were 2-fold less than those achieved previously, we changed the injection procedure and analysed, in 740 ICSI attempts, the importance of interfering technical factors and how to establish a successful ICSI programme, A remarkable change in the fertilization rate up to 68% (595 cycles) occurred when two steps in the injection procedure were performed well, i.e. immobilization of the spermatozoon and placement of the spermatozoon into the ooplasm after cytoplasmic aspiration into the pipette until oolema rupture. This immobilization, by touching the tail with the pipette, is mandatory for increasing the percentage of fertilization, even with totally non-motile spermatozoa (41%). Because aspiration of the cytoplasm is an invasive part of the ICSI procedure and influences the quality of the embryos, it is essential to reduce the amount of cytoplasm drawn into the pipette. This could be attained by using a spikeless injection pipette with the smallest possible internal diameter.</description><subject>Biological and medical sciences</subject><subject>Birth control</subject><subject>Cytoplasm</subject><subject>cytoplasmic aspiration</subject><subject>Embryo Transfer</subject><subject>Embryonic and Fetal Development</subject><subject>Female</subject><subject>Fertilization in Vitro - methods</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>ICSI</subject><subject>immobilization of the spermatozoon</subject><subject>immotile spermatozoa</subject><subject>Male</subject><subject>male factor infertility</subject><subject>Medical sciences</subject><subject>Microinjections - instrumentation</subject><subject>Microinjections - methods</subject><subject>Oocytes</subject><subject>Pregnancy</subject><subject>Sperm Motility</subject><subject>Spermatozoa</subject><subject>Sterility. Assisted procreation</subject><issn>0268-1161</issn><issn>1460-2350</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU-LFDEQxYMo67h69iTkIB6EnsmfTrrH27KsM7Ir6rIL4iWkMxXMmu60SRrd_QJ-bTP0MF6FwCvq_VIFrxB6ScmSkjVfbW8_Xl98XlG65EtRk0doQWtJKsYFeYwWhMm2olTSp-hZSneElLKVJ-iklQ1lki3Qn5tfAUNKMGSnPU4ZxoRtiFjjNBlTHDt57IYctbnPYfQ69c7gNELsS_sOTHZhePevxMFi1_ehc949wG4mdQ4PQWNtM0QcpzFPEfZg_l4keOj1c_TEap_gxUFP0e37i5vzbXX1afPh_OyqMoLQXHFWC74DQkRrLW9aIQ0XwnaWmro807GONVJzQynUZg8VrSWD4pIdM_wUvZnnjjH8nCBl1btkwHs9QJiSaloqZLNeF3A1gyaGlCJYNUbX63ivKFH76NUcvaJUcVWiLz9eHUZPXQ-7I3_IuvivD75ORnsb9WBcOmKcSNYwXrC3Mxam8T92VjPsyuV-H3EdfyjZ8Eao7ddvqhbXXzaby0vV8r91p6vl</recordid><startdate>19960301</startdate><enddate>19960301</enddate><creator>Vanderzwalmen, P.</creator><creator>Bertin, G.</creator><creator>Lejeune, B.</creator><creator>Nijs, M.</creator><creator>Vandamme, B.</creator><creator>Schoysman, R.</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19960301</creationdate><title>Two essential steps for a successful intracytoplasmic sperm injection: injection of immobilized spermatozoa after rupture of the oolema</title><author>Vanderzwalmen, P. ; Bertin, G. ; Lejeune, B. ; Nijs, M. ; Vandamme, B. ; Schoysman, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c501t-32453de0058ff37856c355fbf1c41c4cb2b276a3c11e4c58ff1e4462e41c0d2c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Biological and medical sciences</topic><topic>Birth control</topic><topic>Cytoplasm</topic><topic>cytoplasmic aspiration</topic><topic>Embryo Transfer</topic><topic>Embryonic and Fetal Development</topic><topic>Female</topic><topic>Fertilization in Vitro - methods</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>ICSI</topic><topic>immobilization of the spermatozoon</topic><topic>immotile spermatozoa</topic><topic>Male</topic><topic>male factor infertility</topic><topic>Medical sciences</topic><topic>Microinjections - instrumentation</topic><topic>Microinjections - methods</topic><topic>Oocytes</topic><topic>Pregnancy</topic><topic>Sperm Motility</topic><topic>Spermatozoa</topic><topic>Sterility. Assisted procreation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vanderzwalmen, P.</creatorcontrib><creatorcontrib>Bertin, G.</creatorcontrib><creatorcontrib>Lejeune, B.</creatorcontrib><creatorcontrib>Nijs, M.</creatorcontrib><creatorcontrib>Vandamme, B.</creatorcontrib><creatorcontrib>Schoysman, R.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Human reproduction (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vanderzwalmen, P.</au><au>Bertin, G.</au><au>Lejeune, B.</au><au>Nijs, M.</au><au>Vandamme, B.</au><au>Schoysman, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Two essential steps for a successful intracytoplasmic sperm injection: injection of immobilized spermatozoa after rupture of the oolema</atitle><jtitle>Human reproduction (Oxford)</jtitle><addtitle>Hum Reprod</addtitle><date>1996-03-01</date><risdate>1996</risdate><volume>11</volume><issue>3</issue><spage>540</spage><epage>547</epage><pages>540-547</pages><issn>0268-1161</issn><eissn>1460-2350</eissn><coden>HUREEE</coden><abstract>A total of 740 cycles of intracytoplasmic sperm injection (ICSI) were performed: 625 cycles when <6×105 total motile spermatozoa were harvestable from the ejaculate and 115 cycles in cases with a history of previous fertilization failure after classic in-vitro fertilization or subzonal sperm injection. An average of two pronuclei were observed in 63% of the injected oocytes, allowing 725 transfers of a maximum of three embryos (98%). Of 214 pregnancies initiated, 179 were established (25% of ICSI attempts). Because the fertilization rates from our initial 80 ICSI cycles were 2-fold less than those achieved previously, we changed the injection procedure and analysed, in 740 ICSI attempts, the importance of interfering technical factors and how to establish a successful ICSI programme, A remarkable change in the fertilization rate up to 68% (595 cycles) occurred when two steps in the injection procedure were performed well, i.e. immobilization of the spermatozoon and placement of the spermatozoon into the ooplasm after cytoplasmic aspiration into the pipette until oolema rupture. This immobilization, by touching the tail with the pipette, is mandatory for increasing the percentage of fertilization, even with totally non-motile spermatozoa (41%). Because aspiration of the cytoplasm is an invasive part of the ICSI procedure and influences the quality of the embryos, it is essential to reduce the amount of cytoplasm drawn into the pipette. This could be attained by using a spikeless injection pipette with the smallest possible internal diameter.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>8671262</pmid><doi>10.1093/HUMREP/11.3.540</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0268-1161 |
| ispartof | Human reproduction (Oxford), 1996-03, Vol.11 (3), p.540-547 |
| issn | 0268-1161 1460-2350 |
| language | eng |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Biological and medical sciences Birth control Cytoplasm cytoplasmic aspiration Embryo Transfer Embryonic and Fetal Development Female Fertilization in Vitro - methods Gynecology. Andrology. Obstetrics Humans ICSI immobilization of the spermatozoon immotile spermatozoa Male male factor infertility Medical sciences Microinjections - instrumentation Microinjections - methods Oocytes Pregnancy Sperm Motility Spermatozoa Sterility. Assisted procreation |
| title | Two essential steps for a successful intracytoplasmic sperm injection: injection of immobilized spermatozoa after rupture of the oolema |
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