The effects of detergent on the enzyme-linked immunosorbent assay (ELISA) of blood group substances

The detergents 1-0-n- octyl-β- D- glucopyranoside (OBG) and sodium n-dodecyl sulphate (SDS) have been used to extract blood group substances from human erythrocyte membranse for detection by enzyme-linked immunosorbent assay (ELISA). The effect of detergent concentration on the extraction process an...

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Veröffentlicht in:	Journal of immunological methods 1988-04, Vol.108 (1), p.129-135
Hauptverfasser:	McCabe, J.P., Fletcher, S.M., Jones, M.N.
Format:	Artikel
Sprache:	eng
Schlagworte:	ABO Blood-Group System Binding, Competitive Biological and medical sciences Blood Group Antigens Deoxycholic Acid - pharmacology Detergent-solubilized membrane protein Detergents - pharmacology ELISA Enzyme-Linked Immunosorbent Assay Erythrocyte membrane Erythrocyte Membrane - analysis Erythrocyte Membrane - drug effects Female Fundamental and applied biological sciences. Psychology Fundamental immunology Glucosides - pharmacology Glycosides - pharmacology Humans Molecular immunology Ovarian cyst glycoprotein blood group activity Ovarian Cysts - metabolism Sodium Dodecyl Sulfate - pharmacology Surface-Active Agents - pharmacology Techniques
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