The precursor of PsaD assembles into the photosystem I complex in two steps
The present study addresses the assembly in the chloroplast thylakoid membranes of PsaD, a peripheral membrane protein of the photosystem I complex. Located on the stromal side of the thylakoids, PsaD was found to assemble in vitro into the membranes in its precursor (pre-PsaD) and also in its matur...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1996-06, Vol.93 (13), p.6338-6342 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Minai, L. (The Hebrew University of Jerusalen, Jerusalem, Israel.) Cohen, Y Chitnis, P.R Nechushtai, R |
| description | The present study addresses the assembly in the chloroplast thylakoid membranes of PsaD, a peripheral membrane protein of the photosystem I complex. Located on the stromal side of the thylakoids, PsaD was found to assemble in vitro into the membranes in its precursor (pre-PsaD) and also in its mature (PsaD) form. Newly assembled unprocessed pre-PsaD was resistant to NaBr and alkaline wash. Yet it was sensitive to proteolytic digestion. In contradistinction, when the assembled precursor was processed, the resulting mature PsaD was resistant to proteases to the same extent as endogeneous PsaD. The accumulation of protease-resistant PsaD in the thylakoids correlated with the increase of mature-PsaD in the membranes. This protection of mature PsaD from proteolysis could not be observed when PsaD was in a soluble form--i.e. not assembled within the thylakoids. The data suggest that pre-PsaD assembles to the membranes and only in a second step processing takes place. The observation that the assembly of pre-PsaD is affected by salts to a much lesser extent than that of mature-PsaD supports a two-step assembly of pre-PsaD |
| doi_str_mv | 10.1073/pnas.93.13.6338 |
| format | Article |
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(The Hebrew University of Jerusalen, Jerusalem, Israel.) ; Cohen, Y ; Chitnis, P.R ; Nechushtai, R</creator><creatorcontrib>Minai, L. (The Hebrew University of Jerusalen, Jerusalem, Israel.) ; Cohen, Y ; Chitnis, P.R ; Nechushtai, R</creatorcontrib><description>The present study addresses the assembly in the chloroplast thylakoid membranes of PsaD, a peripheral membrane protein of the photosystem I complex. Located on the stromal side of the thylakoids, PsaD was found to assemble in vitro into the membranes in its precursor (pre-PsaD) and also in its mature (PsaD) form. Newly assembled unprocessed pre-PsaD was resistant to NaBr and alkaline wash. Yet it was sensitive to proteolytic digestion. In contradistinction, when the assembled precursor was processed, the resulting mature PsaD was resistant to proteases to the same extent as endogeneous PsaD. The accumulation of protease-resistant PsaD in the thylakoids correlated with the increase of mature-PsaD in the membranes. This protection of mature PsaD from proteolysis could not be observed when PsaD was in a soluble form--i.e. not assembled within the thylakoids. The data suggest that pre-PsaD assembles to the membranes and only in a second step processing takes place. The observation that the assembly of pre-PsaD is affected by salts to a much lesser extent than that of mature-PsaD supports a two-step assembly of pre-PsaD</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.93.13.6338</identifier><identifier>PMID: 8692816</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Botany ; Chlorophylls ; Chloroplasts ; Flowers & plants ; FOTOSISTEMAS ; Hydrolysis ; IMMUNOLOGIE ; Imports ; INMUNOLOGIA ; Intracellular Membranes - metabolism ; Membrane proteins ; Membranes ; Photosynthetic Reaction Center Complex Proteins - metabolism ; Photosystem I Protein Complex ; PHOTOSYSTEME ; Plant Proteins - metabolism ; Plants ; PLASTE ; PLASTIDIOS ; Plastids ; Protein precursors ; Protein Precursors - metabolism ; Proteins ; PROTEOLISIS ; PROTEOLYSE ; SINTESIS DE PROTEINAS ; Spinach ; SPINACIA OLERACEA ; Spinacia oleracea - metabolism ; SYNTHESE PROTEIQUE ; Thylakoids</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1996-06, Vol.93 (13), p.6338-6342</ispartof><rights>Copyright 1996 National Academy of Sciences</rights><rights>Copyright National Academy of Sciences Jun 25, 1996</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c506t-f84c09b3c5f3d430512bf1c0c3b1e6f2ba68d1d917a7cf839f4b1ac54798f9263</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/93/13.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/39306$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/39306$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27923,27924,53790,53792,58016,58249</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8692816$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Minai, L. (The Hebrew University of Jerusalen, Jerusalem, Israel.)</creatorcontrib><creatorcontrib>Cohen, Y</creatorcontrib><creatorcontrib>Chitnis, P.R</creatorcontrib><creatorcontrib>Nechushtai, R</creatorcontrib><title>The precursor of PsaD assembles into the photosystem I complex in two steps</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The present study addresses the assembly in the chloroplast thylakoid membranes of PsaD, a peripheral membrane protein of the photosystem I complex. Located on the stromal side of the thylakoids, PsaD was found to assemble in vitro into the membranes in its precursor (pre-PsaD) and also in its mature (PsaD) form. Newly assembled unprocessed pre-PsaD was resistant to NaBr and alkaline wash. Yet it was sensitive to proteolytic digestion. In contradistinction, when the assembled precursor was processed, the resulting mature PsaD was resistant to proteases to the same extent as endogeneous PsaD. The accumulation of protease-resistant PsaD in the thylakoids correlated with the increase of mature-PsaD in the membranes. This protection of mature PsaD from proteolysis could not be observed when PsaD was in a soluble form--i.e. not assembled within the thylakoids. The data suggest that pre-PsaD assembles to the membranes and only in a second step processing takes place. The observation that the assembly of pre-PsaD is affected by salts to a much lesser extent than that of mature-PsaD supports a two-step assembly of pre-PsaD</description><subject>Botany</subject><subject>Chlorophylls</subject><subject>Chloroplasts</subject><subject>Flowers & plants</subject><subject>FOTOSISTEMAS</subject><subject>Hydrolysis</subject><subject>IMMUNOLOGIE</subject><subject>Imports</subject><subject>INMUNOLOGIA</subject><subject>Intracellular Membranes - metabolism</subject><subject>Membrane proteins</subject><subject>Membranes</subject><subject>Photosynthetic Reaction Center Complex Proteins - metabolism</subject><subject>Photosystem I Protein Complex</subject><subject>PHOTOSYSTEME</subject><subject>Plant Proteins - metabolism</subject><subject>Plants</subject><subject>PLASTE</subject><subject>PLASTIDIOS</subject><subject>Plastids</subject><subject>Protein precursors</subject><subject>Protein Precursors - metabolism</subject><subject>Proteins</subject><subject>PROTEOLISIS</subject><subject>PROTEOLYSE</subject><subject>SINTESIS DE PROTEINAS</subject><subject>Spinach</subject><subject>SPINACIA OLERACEA</subject><subject>Spinacia oleracea - metabolism</subject><subject>SYNTHESE PROTEIQUE</subject><subject>Thylakoids</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFrFDEUh4Moda2eBUEJHvQ025d52UwCXqRWLRYUbM8hk026s8xMxiSj7X_fGXbdqgdPgXzf7_EeP0KeM1gyqPBk6E1aKlwyXApE-YAsGChWCK7gIVkAlFUheckfkycpbQFArSQckSMpVCmZWJAvlxtHh-jsGFOINHj6LZkP1KTkurp1iTZ9DjTP0ibkkG5Tdh09pzZ0Q-tuJkzzr0Cn3yE9JY-8aZN7tn-PydXHs8vTz8XF10_np-8vCrsCkQsvuQVVo115XHOEFStrzyxYrJkTvqyNkGu2VqwylfUSlec1M3bFKyW9KgUek3e7ucNYd25tXZ-jafUQm87EWx1Mo_8mfbPR1-GnRgUlTvE3-3gMP0aXsu6aZF3bmt6FMelKMg7I-SS-_kfchjH202m6BIYcOM7TTnaSjSGl6PxhDwZ6rkjPFWmFmqGeK5oSL_9c_-DvO5n42z2fg7_p_QDtx7bN7iZP5qv_mpPwYidsUw7xYKBCEPfQm6DNdWySvvquRCm4VHgHSOS24A</recordid><startdate>19960625</startdate><enddate>19960625</enddate><creator>Minai, L. 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(The Hebrew University of Jerusalen, Jerusalem, Israel.)</au><au>Cohen, Y</au><au>Chitnis, P.R</au><au>Nechushtai, R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The precursor of PsaD assembles into the photosystem I complex in two steps</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1996-06-25</date><risdate>1996</risdate><volume>93</volume><issue>13</issue><spage>6338</spage><epage>6342</epage><pages>6338-6342</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>The present study addresses the assembly in the chloroplast thylakoid membranes of PsaD, a peripheral membrane protein of the photosystem I complex. Located on the stromal side of the thylakoids, PsaD was found to assemble in vitro into the membranes in its precursor (pre-PsaD) and also in its mature (PsaD) form. Newly assembled unprocessed pre-PsaD was resistant to NaBr and alkaline wash. Yet it was sensitive to proteolytic digestion. In contradistinction, when the assembled precursor was processed, the resulting mature PsaD was resistant to proteases to the same extent as endogeneous PsaD. The accumulation of protease-resistant PsaD in the thylakoids correlated with the increase of mature-PsaD in the membranes. This protection of mature PsaD from proteolysis could not be observed when PsaD was in a soluble form--i.e. not assembled within the thylakoids. The data suggest that pre-PsaD assembles to the membranes and only in a second step processing takes place. The observation that the assembly of pre-PsaD is affected by salts to a much lesser extent than that of mature-PsaD supports a two-step assembly of pre-PsaD</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>8692816</pmid><doi>10.1073/pnas.93.13.6338</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1996-06, Vol.93 (13), p.6338-6342 |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Botany Chlorophylls Chloroplasts Flowers & plants FOTOSISTEMAS Hydrolysis IMMUNOLOGIE Imports INMUNOLOGIA Intracellular Membranes - metabolism Membrane proteins Membranes Photosynthetic Reaction Center Complex Proteins - metabolism Photosystem I Protein Complex PHOTOSYSTEME Plant Proteins - metabolism Plants PLASTE PLASTIDIOS Plastids Protein precursors Protein Precursors - metabolism Proteins PROTEOLISIS PROTEOLYSE SINTESIS DE PROTEINAS Spinach SPINACIA OLERACEA Spinacia oleracea - metabolism SYNTHESE PROTEIQUE Thylakoids |
| title | The precursor of PsaD assembles into the photosystem I complex in two steps |
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