Detection of lytic antimycobacterial antibodies by immune lysis of liposomes sensitized to tuberculin

Two procedures were used in order to incorporate purified protein derivative tuberculin (PPD) from M. tuberculosis, strain H37Rv, into calcein-containing liposomes: formation of multilamellar vesicles (MLV) in a PPD solution or exposure of preformed MLV to this solution. Immune lysis of these PPD-se...

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Veröffentlicht in:	Journal of immunological methods 1988-04, Vol.108 (1), p.223-230
Hauptverfasser:	Legros, Franz, Castillo, Manuel, Praet, Michel, Vandenbranden, Michel, Lemoine, Olivier, Cabiaux, Véronique, Van Vooren, Jean-Paul, Nyabenda, Jean, Dierckx, Paul, Turneer, Mireille, Ruysschaert, Jean-Marie
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Bacterial - analysis Antibodies, Bacterial - physiology Bacteriology Biological and medical sciences Female Fluoresceins - metabolism Fundamental and applied biological sciences. Psychology human Humans Immune lysis Immune Sera - pharmacology Immunoassay - methods Kinetics Liposomes - immunology Lytic antimycobacterial antibody Microbiology Mycobacterium tuberculosis - immunology Rabbits Sheep Tuberculin - immunology Tuberculoprotein-bearing liposome Tuberculosis Tuberculosis - immunology Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
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