Determination of wogonin in rat plasma by liquid chromatography and its pharmacokinetic application
Abstract A high-performance liquid chromatographic method has been developed for the determination of wogonin in the rat plasma. After addition of an internal standard (clomipramine), plasma was deproteinized by acetonitrile and centrifuged for sample clean-up. The supernatant was separated by a rev...
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| Veröffentlicht in: | Planta medica 1996-06, Vol.62 (3), p.263-266 |
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| container_title | Planta medica |
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| creator | Tsai, T.H Chou, C.J Tsai, T.R Chen, C.F. (National Research Institute of Chinese Medicine, Taipei (Taiwan). Dept. of Pharmacology) |
| description | Abstract
A high-performance liquid chromatographic method has been developed for the determination of wogonin in the rat plasma. After addition of an internal standard (clomipramine), plasma was deproteinized by acetonitrile and centrifuged for sample clean-up. The supernatant was separated by a reversed-phase column chromatography, identified by a photodiode-array detector, and quantified by ultraviolet detection at a wavelength of 275 nm. Acetonitrile-water-diethylamine (50:50:0.1, v/v/v, pH 3.0 adjusted by orthophosphoric acid) was used as the mobile phase. The method was applied to the pharmacokinetic study of wogonin in rats after a dose of 5 mg/kg by intravenous administration. A biphasic phenomenon with a rapid distribution followed by a slower elimination phase was observed from the plasma concentration time profile. |
| doi_str_mv | 10.1055/s-2006-957874 |
| format | Article |
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A high-performance liquid chromatographic method has been developed for the determination of wogonin in the rat plasma. After addition of an internal standard (clomipramine), plasma was deproteinized by acetonitrile and centrifuged for sample clean-up. The supernatant was separated by a reversed-phase column chromatography, identified by a photodiode-array detector, and quantified by ultraviolet detection at a wavelength of 275 nm. Acetonitrile-water-diethylamine (50:50:0.1, v/v/v, pH 3.0 adjusted by orthophosphoric acid) was used as the mobile phase. The method was applied to the pharmacokinetic study of wogonin in rats after a dose of 5 mg/kg by intravenous administration. A biphasic phenomenon with a rapid distribution followed by a slower elimination phase was observed from the plasma concentration time profile.</description><identifier>ISSN: 0032-0943</identifier><identifier>EISSN: 1439-0221</identifier><identifier>DOI: 10.1055/s-2006-957874</identifier><identifier>PMID: 8693042</identifier><identifier>CODEN: PLMEAA</identifier><language>eng</language><publisher>Stuttgart: Thieme</publisher><subject>Analysis ; Animals ; Biological and medical sciences ; CHEMICAL COMPOSITION ; CHEMICAL STRUCTURE ; Chromatography, High Pressure Liquid - methods ; COMPOSICION QUIMICA ; COMPOSITION CHIMIQUE ; DRUG PLANTS ; Drugs, Chinese Herbal - pharmacokinetics ; ESTRUCTURA QUIMICA ; FARMACOLOGIA ; Flavanones ; Flavonoids - blood ; Flavonoids - pharmacokinetics ; General pharmacology ; LABIATAE ; Male ; Medical sciences ; PHARMACOLOGIE ; PHARMACOLOGY ; Pharmacology. Drug treatments ; Plant Roots ; PLANTAS MEDICINALES ; PLANTE MEDICINALE ; RACINE ; RAICES ; Rats ; Rats, Sprague-Dawley ; Reproducibility of Results ; ROOTS ; STRUCTURE CHIMIQUE</subject><ispartof>Planta medica, 1996-06, Vol.62 (3), p.263-266</ispartof><rights>Georg Thieme Verlag Stuttgart · New York</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c373t-a8e5d2ebe6ef223bb780b810940e30b73422c91807a1621cf1fba3595c30ecf73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.thieme-connect.de/products/ejournals/pdf/10.1055/s-2006-957874.pdf$$EPDF$$P50$$Gthieme$$H</linktopdf><link.rule.ids>314,780,784,3017,3018,27924,27925,54559</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3118713$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8693042$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tsai, T.H</creatorcontrib><creatorcontrib>Chou, C.J</creatorcontrib><creatorcontrib>Tsai, T.R</creatorcontrib><creatorcontrib>Chen, C.F. (National Research Institute of Chinese Medicine, Taipei (Taiwan). Dept. of Pharmacology)</creatorcontrib><title>Determination of wogonin in rat plasma by liquid chromatography and its pharmacokinetic application</title><title>Planta medica</title><addtitle>Planta Med</addtitle><description>Abstract
A high-performance liquid chromatographic method has been developed for the determination of wogonin in the rat plasma. After addition of an internal standard (clomipramine), plasma was deproteinized by acetonitrile and centrifuged for sample clean-up. The supernatant was separated by a reversed-phase column chromatography, identified by a photodiode-array detector, and quantified by ultraviolet detection at a wavelength of 275 nm. Acetonitrile-water-diethylamine (50:50:0.1, v/v/v, pH 3.0 adjusted by orthophosphoric acid) was used as the mobile phase. The method was applied to the pharmacokinetic study of wogonin in rats after a dose of 5 mg/kg by intravenous administration. A biphasic phenomenon with a rapid distribution followed by a slower elimination phase was observed from the plasma concentration time profile.</description><subject>Analysis</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>CHEMICAL COMPOSITION</subject><subject>CHEMICAL STRUCTURE</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>COMPOSICION QUIMICA</subject><subject>COMPOSITION CHIMIQUE</subject><subject>DRUG PLANTS</subject><subject>Drugs, Chinese Herbal - pharmacokinetics</subject><subject>ESTRUCTURA QUIMICA</subject><subject>FARMACOLOGIA</subject><subject>Flavanones</subject><subject>Flavonoids - blood</subject><subject>Flavonoids - pharmacokinetics</subject><subject>General pharmacology</subject><subject>LABIATAE</subject><subject>Male</subject><subject>Medical sciences</subject><subject>PHARMACOLOGIE</subject><subject>PHARMACOLOGY</subject><subject>Pharmacology. Drug treatments</subject><subject>Plant Roots</subject><subject>PLANTAS MEDICINALES</subject><subject>PLANTE MEDICINALE</subject><subject>RACINE</subject><subject>RAICES</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reproducibility of Results</subject><subject>ROOTS</subject><subject>STRUCTURE CHIMIQUE</subject><issn>0032-0943</issn><issn>1439-0221</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1v1DAQhi0EKtvCkQsSkg-IE4GxnaydY2nLh1SJC5ytidfedUns1HaE9t_jNqvekEaawzx6Z-Yh5A2DTwy67nNuOMC26TupZPuMbFgr-gY4Z8_JBkDwBvpWvCTnOd8BsLYHOCNnatsLaPmGmGtbbJp8wOJjoNHRv3Efgw-0VsJC5xHzhHQ40tHfL35HzSHFCUvcJ5wPR4phR33JdD5gmtDEPz7Y4g3FeR69eUx9RV44HLN9feoX5PfXm19X35vbn99-XF3eNkZIURpUtttxO9itdZyLYZAKBsXq-WAFDFK0nJueKZDItpwZx9yAous7I8AaJ8UF-bDmzineLzYXPfls7DhisHHJWiomFGvbCjYraFLMOVmn5-QnTEfNQD9I1Vk_SNWr1Mq_OwUvw2R3T_TJYp2_P80xGxxdwmB8fsIEY0oyUbGPK1YO3k5W38UlhSrkv1vfrrjDqHGfauL1Ta--AK9__AM8VJXT</recordid><startdate>19960601</startdate><enddate>19960601</enddate><creator>Tsai, T.H</creator><creator>Chou, C.J</creator><creator>Tsai, T.R</creator><creator>Chen, C.F. (National Research Institute of Chinese Medicine, Taipei (Taiwan). Dept. of Pharmacology)</creator><general>Thieme</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19960601</creationdate><title>Determination of wogonin in rat plasma by liquid chromatography and its pharmacokinetic application</title><author>Tsai, T.H ; Chou, C.J ; Tsai, T.R ; Chen, C.F. (National Research Institute of Chinese Medicine, Taipei (Taiwan). Dept. of Pharmacology)</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c373t-a8e5d2ebe6ef223bb780b810940e30b73422c91807a1621cf1fba3595c30ecf73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Analysis</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>CHEMICAL COMPOSITION</topic><topic>CHEMICAL STRUCTURE</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>COMPOSICION QUIMICA</topic><topic>COMPOSITION CHIMIQUE</topic><topic>DRUG PLANTS</topic><topic>Drugs, Chinese Herbal - pharmacokinetics</topic><topic>ESTRUCTURA QUIMICA</topic><topic>FARMACOLOGIA</topic><topic>Flavanones</topic><topic>Flavonoids - blood</topic><topic>Flavonoids - pharmacokinetics</topic><topic>General pharmacology</topic><topic>LABIATAE</topic><topic>Male</topic><topic>Medical sciences</topic><topic>PHARMACOLOGIE</topic><topic>PHARMACOLOGY</topic><topic>Pharmacology. Drug treatments</topic><topic>Plant Roots</topic><topic>PLANTAS MEDICINALES</topic><topic>PLANTE MEDICINALE</topic><topic>RACINE</topic><topic>RAICES</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reproducibility of Results</topic><topic>ROOTS</topic><topic>STRUCTURE CHIMIQUE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tsai, T.H</creatorcontrib><creatorcontrib>Chou, C.J</creatorcontrib><creatorcontrib>Tsai, T.R</creatorcontrib><creatorcontrib>Chen, C.F. (National Research Institute of Chinese Medicine, Taipei (Taiwan). Dept. of Pharmacology)</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Planta medica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tsai, T.H</au><au>Chou, C.J</au><au>Tsai, T.R</au><au>Chen, C.F. (National Research Institute of Chinese Medicine, Taipei (Taiwan). Dept. of Pharmacology)</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of wogonin in rat plasma by liquid chromatography and its pharmacokinetic application</atitle><jtitle>Planta medica</jtitle><addtitle>Planta Med</addtitle><date>1996-06-01</date><risdate>1996</risdate><volume>62</volume><issue>3</issue><spage>263</spage><epage>266</epage><pages>263-266</pages><issn>0032-0943</issn><eissn>1439-0221</eissn><coden>PLMEAA</coden><abstract>Abstract
A high-performance liquid chromatographic method has been developed for the determination of wogonin in the rat plasma. After addition of an internal standard (clomipramine), plasma was deproteinized by acetonitrile and centrifuged for sample clean-up. The supernatant was separated by a reversed-phase column chromatography, identified by a photodiode-array detector, and quantified by ultraviolet detection at a wavelength of 275 nm. Acetonitrile-water-diethylamine (50:50:0.1, v/v/v, pH 3.0 adjusted by orthophosphoric acid) was used as the mobile phase. The method was applied to the pharmacokinetic study of wogonin in rats after a dose of 5 mg/kg by intravenous administration. A biphasic phenomenon with a rapid distribution followed by a slower elimination phase was observed from the plasma concentration time profile.</abstract><cop>Stuttgart</cop><cop>New York, NY</cop><pub>Thieme</pub><pmid>8693042</pmid><doi>10.1055/s-2006-957874</doi><tpages>4</tpages></addata></record> |
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| language | eng |
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| source | MEDLINE; Thieme Connect Journals |
| subjects | Analysis Animals Biological and medical sciences CHEMICAL COMPOSITION CHEMICAL STRUCTURE Chromatography, High Pressure Liquid - methods COMPOSICION QUIMICA COMPOSITION CHIMIQUE DRUG PLANTS Drugs, Chinese Herbal - pharmacokinetics ESTRUCTURA QUIMICA FARMACOLOGIA Flavanones Flavonoids - blood Flavonoids - pharmacokinetics General pharmacology LABIATAE Male Medical sciences PHARMACOLOGIE PHARMACOLOGY Pharmacology. Drug treatments Plant Roots PLANTAS MEDICINALES PLANTE MEDICINALE RACINE RAICES Rats Rats, Sprague-Dawley Reproducibility of Results ROOTS STRUCTURE CHIMIQUE |
| title | Determination of wogonin in rat plasma by liquid chromatography and its pharmacokinetic application |
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