Manduca sexta hemolymph ferritin: cDNA sequence and mRNA expression
A cDNA clone encoding a subunit of the tobacco hornworm Manduca sexta ( Ms) hemolymph (serum) ferritin (Fer) has been identified and sequenced. The deduced amino acid (aa) sequence shows approx. 50% similarity to vertebrate Fer subunit sequences, and the nucleotide sequence contains a stem-loop stru...
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| Veröffentlicht in: | Gene 1996-06, Vol.172 (2), p.255-259 |
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| creator | Pham, Daphne Q.-D. Zhang, Dianzheng Hufnagel, David H. Winzerling, Joy J. |
| description | A cDNA clone encoding a subunit of the tobacco hornworm
Manduca sexta (
Ms) hemolymph (serum) ferritin (Fer) has been identified and sequenced. The deduced amino acid (aa) sequence shows approx. 50% similarity to vertebrate Fer subunit sequences, and the nucleotide sequence contains a stem-loop structure in the 5′ untranslated region that could serve as an iron-responsive element (
IRE). The stem-loop of this putative
IRE exhibits high identity to vertebrate
IRE that play an essential role in the control of Fer synthesis. The
Ms Fer subunit lacks one of the three Tyr residues required for the rapid biomineralization of iron shown in vertebrate heavy-chain Fer. In addition, aa residues that comprise the putative ferroxidase centers generally are not conserved, suggesting that the
Ms Fer subunit more closely resembles the vertebrate light-chain subunit. Northern blot analyses indicate that the
fer mRNA is expressed in the midgut, fat body and hemocytes, with the greatest expression in the midgut. |
| doi_str_mv | 10.1016/0378-1119(96)00012-1 |
| format | Article |
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Manduca sexta (
Ms) hemolymph (serum) ferritin (Fer) has been identified and sequenced. The deduced amino acid (aa) sequence shows approx. 50% similarity to vertebrate Fer subunit sequences, and the nucleotide sequence contains a stem-loop structure in the 5′ untranslated region that could serve as an iron-responsive element (
IRE). The stem-loop of this putative
IRE exhibits high identity to vertebrate
IRE that play an essential role in the control of Fer synthesis. The
Ms Fer subunit lacks one of the three Tyr residues required for the rapid biomineralization of iron shown in vertebrate heavy-chain Fer. In addition, aa residues that comprise the putative ferroxidase centers generally are not conserved, suggesting that the
Ms Fer subunit more closely resembles the vertebrate light-chain subunit. Northern blot analyses indicate that the
fer mRNA is expressed in the midgut, fat body and hemocytes, with the greatest expression in the midgut.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(96)00012-1</identifier><identifier>PMID: 8682313</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>ADN ; Amino Acid Sequence ; Animals ; ARN MENSAJERO ; ARN MESSAGER ; Base Sequence ; Blotting, Northern ; circulatory ; Cloning, Molecular ; DNA ; DNA, Complementary ; FER ; Ferritins - blood ; Ferritins - genetics ; Hemolymph ; HIERRO ; Humans ; IRON ; Manduca - genetics ; MANDUCA SEXTA ; MESSENGER RNA ; Molecular Sequence Data ; Northern blot analysis ; nucleic acid ; Nucleic Acid Conformation ; NUCLEOTIDE SEQUENCE ; RNA, Messenger ; SECUENCIA NUCLEOTIDICA ; SEQUENCE NUCLEOTIDIQUE ; serum ; Tobacco hornworm</subject><ispartof>Gene, 1996-06, Vol.172 (2), p.255-259</ispartof><rights>1996</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c407t-e61bdc85ad391ee9b8c0abe13edbcfcc276db2d93f0ff5eb495a00fcca87c8a83</citedby><cites>FETCH-LOGICAL-c407t-e61bdc85ad391ee9b8c0abe13edbcfcc276db2d93f0ff5eb495a00fcca87c8a83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0378111996000121$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8682313$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pham, Daphne Q.-D.</creatorcontrib><creatorcontrib>Zhang, Dianzheng</creatorcontrib><creatorcontrib>Hufnagel, David H.</creatorcontrib><creatorcontrib>Winzerling, Joy J.</creatorcontrib><title>Manduca sexta hemolymph ferritin: cDNA sequence and mRNA expression</title><title>Gene</title><addtitle>Gene</addtitle><description>A cDNA clone encoding a subunit of the tobacco hornworm
Manduca sexta (
Ms) hemolymph (serum) ferritin (Fer) has been identified and sequenced. The deduced amino acid (aa) sequence shows approx. 50% similarity to vertebrate Fer subunit sequences, and the nucleotide sequence contains a stem-loop structure in the 5′ untranslated region that could serve as an iron-responsive element (
IRE). The stem-loop of this putative
IRE exhibits high identity to vertebrate
IRE that play an essential role in the control of Fer synthesis. The
Ms Fer subunit lacks one of the three Tyr residues required for the rapid biomineralization of iron shown in vertebrate heavy-chain Fer. In addition, aa residues that comprise the putative ferroxidase centers generally are not conserved, suggesting that the
Ms Fer subunit more closely resembles the vertebrate light-chain subunit. Northern blot analyses indicate that the
fer mRNA is expressed in the midgut, fat body and hemocytes, with the greatest expression in the midgut.</description><subject>ADN</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>ARN MENSAJERO</subject><subject>ARN MESSAGER</subject><subject>Base Sequence</subject><subject>Blotting, Northern</subject><subject>circulatory</subject><subject>Cloning, Molecular</subject><subject>DNA</subject><subject>DNA, Complementary</subject><subject>FER</subject><subject>Ferritins - blood</subject><subject>Ferritins - genetics</subject><subject>Hemolymph</subject><subject>HIERRO</subject><subject>Humans</subject><subject>IRON</subject><subject>Manduca - genetics</subject><subject>MANDUCA SEXTA</subject><subject>MESSENGER RNA</subject><subject>Molecular Sequence Data</subject><subject>Northern blot analysis</subject><subject>nucleic acid</subject><subject>Nucleic Acid Conformation</subject><subject>NUCLEOTIDE SEQUENCE</subject><subject>RNA, Messenger</subject><subject>SECUENCIA NUCLEOTIDICA</subject><subject>SEQUENCE NUCLEOTIDIQUE</subject><subject>serum</subject><subject>Tobacco hornworm</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFO3DAQhi0EWrbAC1RFyqlqD6Ez8TqxOSChbQtI261Uwdly7AkYbZLFzlbw9jjaFUc4Wfb__TPWx9gXhDMELH8Ar2SOiOqbKr8DABY57rEpykrlAFzus-kbcsg-xfiYIBCimLCJLGXBkU_Z_I_p3MaaLNLzYLIHavvVS7t-yBoKwQ--O8_sz-Vlip821FnKEp61_9ILPa8Dxej77pgdNGYV6WR3HrG7379u59f54u_VzfxykdsZVENOJdbOSmEcV0ikamnB1IScXG0ba4uqdHXhFG-gaQTVMyUMQAqMrKw0kh-xr9u569Cn38RBtz5aWq1MR_0m6koiL0UhPgRRCCk4FgmcbUEb-hgDNXodfGvCi0bQo2Q9GtSjQa3SZZSsMdVOd_M3dUvurbSzmvLP27wxvTb3wUe9XKgKYFaO5YttSEnVf09BR-tHtc4HsoN2vX9_-yueKJPf</recordid><startdate>19960626</startdate><enddate>19960626</enddate><creator>Pham, Daphne Q.-D.</creator><creator>Zhang, Dianzheng</creator><creator>Hufnagel, David H.</creator><creator>Winzerling, Joy J.</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19960626</creationdate><title>Manduca sexta hemolymph ferritin: cDNA sequence and mRNA expression</title><author>Pham, Daphne Q.-D. ; Zhang, Dianzheng ; Hufnagel, David H. ; Winzerling, Joy J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c407t-e61bdc85ad391ee9b8c0abe13edbcfcc276db2d93f0ff5eb495a00fcca87c8a83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>ADN</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>ARN MENSAJERO</topic><topic>ARN MESSAGER</topic><topic>Base Sequence</topic><topic>Blotting, Northern</topic><topic>circulatory</topic><topic>Cloning, Molecular</topic><topic>DNA</topic><topic>DNA, Complementary</topic><topic>FER</topic><topic>Ferritins - blood</topic><topic>Ferritins - genetics</topic><topic>Hemolymph</topic><topic>HIERRO</topic><topic>Humans</topic><topic>IRON</topic><topic>Manduca - genetics</topic><topic>MANDUCA SEXTA</topic><topic>MESSENGER RNA</topic><topic>Molecular Sequence Data</topic><topic>Northern blot analysis</topic><topic>nucleic acid</topic><topic>Nucleic Acid Conformation</topic><topic>NUCLEOTIDE SEQUENCE</topic><topic>RNA, Messenger</topic><topic>SECUENCIA NUCLEOTIDICA</topic><topic>SEQUENCE NUCLEOTIDIQUE</topic><topic>serum</topic><topic>Tobacco hornworm</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pham, Daphne Q.-D.</creatorcontrib><creatorcontrib>Zhang, Dianzheng</creatorcontrib><creatorcontrib>Hufnagel, David H.</creatorcontrib><creatorcontrib>Winzerling, Joy J.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pham, Daphne Q.-D.</au><au>Zhang, Dianzheng</au><au>Hufnagel, David H.</au><au>Winzerling, Joy J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Manduca sexta hemolymph ferritin: cDNA sequence and mRNA expression</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1996-06-26</date><risdate>1996</risdate><volume>172</volume><issue>2</issue><spage>255</spage><epage>259</epage><pages>255-259</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>A cDNA clone encoding a subunit of the tobacco hornworm
Manduca sexta (
Ms) hemolymph (serum) ferritin (Fer) has been identified and sequenced. The deduced amino acid (aa) sequence shows approx. 50% similarity to vertebrate Fer subunit sequences, and the nucleotide sequence contains a stem-loop structure in the 5′ untranslated region that could serve as an iron-responsive element (
IRE). The stem-loop of this putative
IRE exhibits high identity to vertebrate
IRE that play an essential role in the control of Fer synthesis. The
Ms Fer subunit lacks one of the three Tyr residues required for the rapid biomineralization of iron shown in vertebrate heavy-chain Fer. In addition, aa residues that comprise the putative ferroxidase centers generally are not conserved, suggesting that the
Ms Fer subunit more closely resembles the vertebrate light-chain subunit. Northern blot analyses indicate that the
fer mRNA is expressed in the midgut, fat body and hemocytes, with the greatest expression in the midgut.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>8682313</pmid><doi>10.1016/0378-1119(96)00012-1</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Gene, 1996-06, Vol.172 (2), p.255-259 |
| issn | 0378-1119 1879-0038 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | ADN Amino Acid Sequence Animals ARN MENSAJERO ARN MESSAGER Base Sequence Blotting, Northern circulatory Cloning, Molecular DNA DNA, Complementary FER Ferritins - blood Ferritins - genetics Hemolymph HIERRO Humans IRON Manduca - genetics MANDUCA SEXTA MESSENGER RNA Molecular Sequence Data Northern blot analysis nucleic acid Nucleic Acid Conformation NUCLEOTIDE SEQUENCE RNA, Messenger SECUENCIA NUCLEOTIDICA SEQUENCE NUCLEOTIDIQUE serum Tobacco hornworm |
| title | Manduca sexta hemolymph ferritin: cDNA sequence and mRNA expression |
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