Sequence Variation as a Strategy for Crystallizing RNA Motifs
The determination of RNA structures by X-ray crystallography is an exciting and developing field. At present, the crystallographic characteriz ation of RNA is limited by the difficulty in obtaining large, high quality crystals. This paper outlines several techniques for improving the likelihood of o...
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| Veröffentlicht in: | Journal of molecular biology 1996-06, Vol.259 (4), p.696-703 |
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| container_end_page | 703 |
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| container_issue | 4 |
| container_start_page | 696 |
| container_title | Journal of molecular biology |
| container_volume | 259 |
| creator | Anderson, Amy C. Earp, Brandon E. Frederick, Christin A. |
| description | The determination of RNA structures by X-ray crystallography is an exciting and developing field. At present, the crystallographic characteriz ation of RNA is limited by the difficulty in obtaining large, high quality crystals. This paper outlines several techniques for improving the likelihood of obtaining RNA crystals, for improving the size of those crystals, and for extending the limit of the diffraction maxima. Sequence variations have proven to be more effective in changing the quality of the crystals than variations in crystallization conditions, often making the difference between obtaining true single crystals and multiply twinned crystalline material. |
| doi_str_mv | 10.1006/jmbi.1996.0351 |
| format | Article |
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Sequence variations have proven to be more effective in changing the quality of the crystals than variations in crystallization conditions, often making the difference between obtaining true single crystals and multiply twinned crystalline material.</description><identifier>ISSN: 0022-2836</identifier><identifier>EISSN: 1089-8638</identifier><identifier>DOI: 10.1006/jmbi.1996.0351</identifier><identifier>PMID: 8683576</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Base Sequence ; Bromine - chemistry ; Crystallization ; Crystallography, X-Ray ; hairpin ; Molecular Sequence Data ; Nucleic Acid Conformation ; oligonucleotide synthesis ; Oligoribonucleotides - chemical synthesis ; Oligoribonucleotides - chemistry ; Oligoribonucleotides - isolation & purification ; pseudoknot ; RNA - chemistry ; RNA structure ; X-ray crystallography</subject><ispartof>Journal of molecular biology, 1996-06, Vol.259 (4), p.696-703</ispartof><rights>1996 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-dfd345c38d6e9c37dd03d227e17f3c23d88b3a03647d0aa3d23e03dd89ab09db3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/jmbi.1996.0351$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8683576$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Anderson, Amy C.</creatorcontrib><creatorcontrib>Earp, Brandon E.</creatorcontrib><creatorcontrib>Frederick, Christin A.</creatorcontrib><title>Sequence Variation as a Strategy for Crystallizing RNA Motifs</title><title>Journal of molecular biology</title><addtitle>J Mol Biol</addtitle><description>The determination of RNA structures by X-ray crystallography is an exciting and developing field. 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Sequence variations have proven to be more effective in changing the quality of the crystals than variations in crystallization conditions, often making the difference between obtaining true single crystals and multiply twinned crystalline material.</description><subject>Base Sequence</subject><subject>Bromine - chemistry</subject><subject>Crystallization</subject><subject>Crystallography, X-Ray</subject><subject>hairpin</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Conformation</subject><subject>oligonucleotide synthesis</subject><subject>Oligoribonucleotides - chemical synthesis</subject><subject>Oligoribonucleotides - chemistry</subject><subject>Oligoribonucleotides - isolation & purification</subject><subject>pseudoknot</subject><subject>RNA - chemistry</subject><subject>RNA structure</subject><subject>X-ray crystallography</subject><issn>0022-2836</issn><issn>1089-8638</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM9LwzAUx4Moc06v3oScvLUmeW2aHjzI8BdMBadeQ5qkI6NdZ5IJ86-3ZcObeHrwvp_35fFB6JySlBLCr5Zt5VJaljwlkNMDNKZElIngIA7RmBDGEiaAH6OTEJaEkBwyMUIjwQXkBR-j67n93NiVtvhDeaei61ZYBazwPHoV7WKL687jqd-GqJrGfbvVAr8-3-CnLro6nKKjWjXBnu3nBL3f3b5NH5LZy_3j9GaWaChITExtIMs1CMNt2a-MIWAYKywtatAMjBAVKAI8KwxRqs_A9oQRpapIaSqYoMtd79p3_bshytYFbZtGrWy3CbIQFGhW5v-CNBcZYzCA6Q7UvgvB21quvWuV30pK5CBWDmLlIFYOYvuDi33zpmqt-cX3Jvtc7HLbe_hy1sug3SDWOG91lKZzf1X_AM87hpM</recordid><startdate>19960621</startdate><enddate>19960621</enddate><creator>Anderson, Amy C.</creator><creator>Earp, Brandon E.</creator><creator>Frederick, Christin A.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19960621</creationdate><title>Sequence Variation as a Strategy for Crystallizing RNA Motifs</title><author>Anderson, Amy C. ; Earp, Brandon E. ; Frederick, Christin A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-dfd345c38d6e9c37dd03d227e17f3c23d88b3a03647d0aa3d23e03dd89ab09db3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Base Sequence</topic><topic>Bromine - chemistry</topic><topic>Crystallization</topic><topic>Crystallography, X-Ray</topic><topic>hairpin</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Conformation</topic><topic>oligonucleotide synthesis</topic><topic>Oligoribonucleotides - chemical synthesis</topic><topic>Oligoribonucleotides - chemistry</topic><topic>Oligoribonucleotides - isolation & purification</topic><topic>pseudoknot</topic><topic>RNA - chemistry</topic><topic>RNA structure</topic><topic>X-ray crystallography</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Anderson, Amy C.</creatorcontrib><creatorcontrib>Earp, Brandon E.</creatorcontrib><creatorcontrib>Frederick, Christin A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Anderson, Amy C.</au><au>Earp, Brandon E.</au><au>Frederick, Christin A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sequence Variation as a Strategy for Crystallizing RNA Motifs</atitle><jtitle>Journal of molecular biology</jtitle><addtitle>J Mol Biol</addtitle><date>1996-06-21</date><risdate>1996</risdate><volume>259</volume><issue>4</issue><spage>696</spage><epage>703</epage><pages>696-703</pages><issn>0022-2836</issn><eissn>1089-8638</eissn><abstract>The determination of RNA structures by X-ray crystallography is an exciting and developing field. 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| fulltext | fulltext |
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| ispartof | Journal of molecular biology, 1996-06, Vol.259 (4), p.696-703 |
| issn | 0022-2836 1089-8638 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78131495 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Base Sequence Bromine - chemistry Crystallization Crystallography, X-Ray hairpin Molecular Sequence Data Nucleic Acid Conformation oligonucleotide synthesis Oligoribonucleotides - chemical synthesis Oligoribonucleotides - chemistry Oligoribonucleotides - isolation & purification pseudoknot RNA - chemistry RNA structure X-ray crystallography |
| title | Sequence Variation as a Strategy for Crystallizing RNA Motifs |
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