Measurement of Natural Rubber Proteins in Latex Glove Extracts: Comparison of the Methods
Healthcare workers and individuals with frequent contact with latex are at risk for latex protein allergy. The purpose of this study was to compare several established methods for measuring protein in extracts from latex-containing medical devices. Extracts from latex gloves were analyzed for natura...
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| Veröffentlicht in: | Annals of allergy, asthma, & immunology asthma, & immunology, 1996-06, Vol.76 (6), p.520-526 |
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| container_title | Annals of allergy, asthma, & immunology |
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| creator | Beezhold, Donald Swanson, Mark Zehr, Bradley D Kostyal, David |
| description | Healthcare workers and individuals with frequent contact with latex are at risk for latex protein allergy.
The purpose of this study was to compare several established methods for measuring protein in extracts from latex-containing medical devices.
Extracts from latex gloves were analyzed for natural rubber proteins using a modified Lowry assay and two different immunochemical assays. The imunochemical methods were competitive inhibition assays that employed either immune rabbit serum or human serum with antibodies directed against natural rubber proteins.
Seventy extracts representing five different brands of gloves from four manufacturers were analyzed. A good linear correlation (r = 0.88) was found between the immunoassay methods. Correlation to the modified Lowry method was not possible because many of the samples were below the limit of detection for the Lowry assay. Reference extracts and antisera were further characterized by Western blot analysis. The data demonstrate that the proteins recognized by rabbit antisera and the proteins recognized by human IgE are similar. The greatest difference in the immunochemical assays appears to be the relative binding of the antibody sources to high and low molecular weight natural rubber proteins in the reference extracts.
The immunochemical assays are specific for latex proteins and provide a more sensitive and biologically relevant method for determining protein levels in latex medical products. |
| doi_str_mv | 10.1016/S1081-1206(10)63271-1 |
| format | Article |
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The purpose of this study was to compare several established methods for measuring protein in extracts from latex-containing medical devices.
Extracts from latex gloves were analyzed for natural rubber proteins using a modified Lowry assay and two different immunochemical assays. The imunochemical methods were competitive inhibition assays that employed either immune rabbit serum or human serum with antibodies directed against natural rubber proteins.
Seventy extracts representing five different brands of gloves from four manufacturers were analyzed. A good linear correlation (r = 0.88) was found between the immunoassay methods. Correlation to the modified Lowry method was not possible because many of the samples were below the limit of detection for the Lowry assay. Reference extracts and antisera were further characterized by Western blot analysis. The data demonstrate that the proteins recognized by rabbit antisera and the proteins recognized by human IgE are similar. The greatest difference in the immunochemical assays appears to be the relative binding of the antibody sources to high and low molecular weight natural rubber proteins in the reference extracts.
The immunochemical assays are specific for latex proteins and provide a more sensitive and biologically relevant method for determining protein levels in latex medical products.</description><identifier>ISSN: 1081-1206</identifier><identifier>EISSN: 1534-4436</identifier><identifier>DOI: 10.1016/S1081-1206(10)63271-1</identifier><identifier>PMID: 8673686</identifier><identifier>CODEN: ANAEA3</identifier><language>eng</language><publisher>McLean, VA: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; Blotting, Western ; Enzyme-Linked Immunosorbent Assay ; Gloves, Protective ; Humans ; Immunoassay - methods ; Investigative techniques, diagnostic techniques (general aspects) ; Latex - analysis ; Latex - chemistry ; Medical sciences ; Miscellaneous. Technology ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Plant Proteins - analysis ; Plant Proteins - chemistry ; Precipitin Tests ; Rabbits ; Reference Standards ; Reference Values ; Rubber - analysis ; Rubber - chemistry</subject><ispartof>Annals of allergy, asthma, & immunology, 1996-06, Vol.76 (6), p.520-526</ispartof><rights>1996 American College of Allergy, Asthma & Immunology</rights><rights>1996 INIST-CNRS</rights><rights>Copyright American College of Allergy and Immunology Jun 1996</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c447t-689c7f38ce131c2cd786e6a40682507e03521114e1d0553f07c09a724d18fc903</citedby><cites>FETCH-LOGICAL-c447t-689c7f38ce131c2cd786e6a40682507e03521114e1d0553f07c09a724d18fc903</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S1081-1206(10)63271-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3130298$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8673686$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Beezhold, Donald</creatorcontrib><creatorcontrib>Swanson, Mark</creatorcontrib><creatorcontrib>Zehr, Bradley D</creatorcontrib><creatorcontrib>Kostyal, David</creatorcontrib><title>Measurement of Natural Rubber Proteins in Latex Glove Extracts: Comparison of the Methods</title><title>Annals of allergy, asthma, & immunology</title><addtitle>Ann Allergy Asthma Immunol</addtitle><description>Healthcare workers and individuals with frequent contact with latex are at risk for latex protein allergy.
The purpose of this study was to compare several established methods for measuring protein in extracts from latex-containing medical devices.
Extracts from latex gloves were analyzed for natural rubber proteins using a modified Lowry assay and two different immunochemical assays. The imunochemical methods were competitive inhibition assays that employed either immune rabbit serum or human serum with antibodies directed against natural rubber proteins.
Seventy extracts representing five different brands of gloves from four manufacturers were analyzed. A good linear correlation (r = 0.88) was found between the immunoassay methods. Correlation to the modified Lowry method was not possible because many of the samples were below the limit of detection for the Lowry assay. Reference extracts and antisera were further characterized by Western blot analysis. The data demonstrate that the proteins recognized by rabbit antisera and the proteins recognized by human IgE are similar. The greatest difference in the immunochemical assays appears to be the relative binding of the antibody sources to high and low molecular weight natural rubber proteins in the reference extracts.
The immunochemical assays are specific for latex proteins and provide a more sensitive and biologically relevant method for determining protein levels in latex medical products.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Gloves, Protective</subject><subject>Humans</subject><subject>Immunoassay - methods</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Latex - analysis</subject><subject>Latex - chemistry</subject><subject>Medical sciences</subject><subject>Miscellaneous. Technology</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Plant Proteins - analysis</subject><subject>Plant Proteins - chemistry</subject><subject>Precipitin Tests</subject><subject>Rabbits</subject><subject>Reference Standards</subject><subject>Reference Values</subject><subject>Rubber - analysis</subject><subject>Rubber - chemistry</subject><issn>1081-1206</issn><issn>1534-4436</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhiMEKm3hJ1SKEELlEJixHcfpBaFVKUhbQHwcOFleZ6K6SuLFdqry73G6Sw9cevLHPPNq3nmL4gThDQLKt98RFFbIQJ4ivJacNfn1qDjEmotKCC4f5_s_5GlxFOM1AKCS_KA4ULLhUsnD4tclmTgHGmlKpe_LzybNwQzlt3mzoVB-DT6Rm2LppnJtEt2WF4O_ofL8NgVjUzwrV37cmuCin5b2dEXlJaUr38VnxZPeDJGe78_j4ueH8x-rj9X6y8Wn1ft1ZYVoUiVVa5ueK0vI0TLbNUqSNAKkYjU0BLxmiCgIO6hr3kNjoTUNEx2q3rbAj4tXO91t8L9nikmPLloaBjORn6NuFHIApR4EsVZKtKLN4Iv_wGs_hymb0AxY1gMmMlTvIBt8jIF6vQ1uNOGPRtBLQPouIL1sf_m6C0hj7jvZi8-bkbr7rn0iuf5yXzfRmqEPZrIu3mM8m2HtYubdDqO82htHQUfraLLUuUA26c67Bwb5C8oUqdA</recordid><startdate>19960601</startdate><enddate>19960601</enddate><creator>Beezhold, Donald</creator><creator>Swanson, Mark</creator><creator>Zehr, Bradley D</creator><creator>Kostyal, David</creator><general>Elsevier Inc</general><general>American College of Allergy, Asthma, & Immunology</general><general>American College of Allergy and Immunology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19960601</creationdate><title>Measurement of Natural Rubber Proteins in Latex Glove Extracts: Comparison of the Methods</title><author>Beezhold, Donald ; Swanson, Mark ; Zehr, Bradley D ; Kostyal, David</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c447t-689c7f38ce131c2cd786e6a40682507e03521114e1d0553f07c09a724d18fc903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Gloves, Protective</topic><topic>Humans</topic><topic>Immunoassay - methods</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Latex - analysis</topic><topic>Latex - chemistry</topic><topic>Medical sciences</topic><topic>Miscellaneous. Technology</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Plant Proteins - analysis</topic><topic>Plant Proteins - chemistry</topic><topic>Precipitin Tests</topic><topic>Rabbits</topic><topic>Reference Standards</topic><topic>Reference Values</topic><topic>Rubber - analysis</topic><topic>Rubber - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Beezhold, Donald</creatorcontrib><creatorcontrib>Swanson, Mark</creatorcontrib><creatorcontrib>Zehr, Bradley D</creatorcontrib><creatorcontrib>Kostyal, David</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of allergy, asthma, & immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Beezhold, Donald</au><au>Swanson, Mark</au><au>Zehr, Bradley D</au><au>Kostyal, David</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Measurement of Natural Rubber Proteins in Latex Glove Extracts: Comparison of the Methods</atitle><jtitle>Annals of allergy, asthma, & immunology</jtitle><addtitle>Ann Allergy Asthma Immunol</addtitle><date>1996-06-01</date><risdate>1996</risdate><volume>76</volume><issue>6</issue><spage>520</spage><epage>526</epage><pages>520-526</pages><issn>1081-1206</issn><eissn>1534-4436</eissn><coden>ANAEA3</coden><abstract>Healthcare workers and individuals with frequent contact with latex are at risk for latex protein allergy.
The purpose of this study was to compare several established methods for measuring protein in extracts from latex-containing medical devices.
Extracts from latex gloves were analyzed for natural rubber proteins using a modified Lowry assay and two different immunochemical assays. The imunochemical methods were competitive inhibition assays that employed either immune rabbit serum or human serum with antibodies directed against natural rubber proteins.
Seventy extracts representing five different brands of gloves from four manufacturers were analyzed. A good linear correlation (r = 0.88) was found between the immunoassay methods. Correlation to the modified Lowry method was not possible because many of the samples were below the limit of detection for the Lowry assay. Reference extracts and antisera were further characterized by Western blot analysis. The data demonstrate that the proteins recognized by rabbit antisera and the proteins recognized by human IgE are similar. The greatest difference in the immunochemical assays appears to be the relative binding of the antibody sources to high and low molecular weight natural rubber proteins in the reference extracts.
The immunochemical assays are specific for latex proteins and provide a more sensitive and biologically relevant method for determining protein levels in latex medical products.</abstract><cop>McLean, VA</cop><pub>Elsevier Inc</pub><pmid>8673686</pmid><doi>10.1016/S1081-1206(10)63271-1</doi><tpages>7</tpages></addata></record> |
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| ispartof | Annals of allergy, asthma, & immunology, 1996-06, Vol.76 (6), p.520-526 |
| issn | 1081-1206 1534-4436 |
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| subjects | Animals Biological and medical sciences Blotting, Western Enzyme-Linked Immunosorbent Assay Gloves, Protective Humans Immunoassay - methods Investigative techniques, diagnostic techniques (general aspects) Latex - analysis Latex - chemistry Medical sciences Miscellaneous. Technology Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Plant Proteins - analysis Plant Proteins - chemistry Precipitin Tests Rabbits Reference Standards Reference Values Rubber - analysis Rubber - chemistry |
| title | Measurement of Natural Rubber Proteins in Latex Glove Extracts: Comparison of the Methods |
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