Uptake of γ‐Aminobutyric Acid by a Synaptic Vesicle Fraction Isolated from Rat Brain
: γ‐Aminobutyric acid (GABA) was taken up by a MgATP‐dependent mechanism into synaptic vesicles isolated by hypoosmotic shock and density gradient centrifugation. The properties of the vesicular uptake differed clearly from those of synaptosomal and glial uptake, both with respect to Na+, Mg2+, and...
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| Veröffentlicht in: | Journal of neurochemistry 1988-04, Vol.50 (4), p.1237-1242 |
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| container_title | Journal of neurochemistry |
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| creator | Fykse, Else M. Fonnum, Frode |
| description | : γ‐Aminobutyric acid (GABA) was taken up by a MgATP‐dependent mechanism into synaptic vesicles isolated by hypoosmotic shock and density gradient centrifugation. The properties of the vesicular uptake differed clearly from those of synaptosomal and glial uptake, both with respect to Na+, Mg2+, and ATP dependence and with respect to response to general GABA uptake inhibitors such as nipecotic acid, diaminobutyric acid, and β‐alanine. The uptake showed a Km of 5.6 mM and a net uptake rate of 1,500 pmol/min/mg of protein. It is suggested that the vesicular uptake of GABA is driven by an electrochemical proton gradient generated by a Mg2+‐ATPase. |
| doi_str_mv | 10.1111/j.1471-4159.1988.tb10599.x |
| format | Article |
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The properties of the vesicular uptake differed clearly from those of synaptosomal and glial uptake, both with respect to Na+, Mg2+, and ATP dependence and with respect to response to general GABA uptake inhibitors such as nipecotic acid, diaminobutyric acid, and β‐alanine. The uptake showed a Km of 5.6 mM and a net uptake rate of 1,500 pmol/min/mg of protein. 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Psychology ; gamma-Aminobutyric Acid - metabolism ; Kinetics ; Magnesium - pharmacology ; Male ; Molecular and cellular biology ; Neurotransmission ; Osmotic Pressure ; Rats ; Rats, Inbred Strains ; Sodium - pharmacology ; Synaptic vesicles ; Synaptic Vesicles - metabolism ; Synaptosomes ; Synaptosomes - metabolism ; γ‐Aminobutyric acid uptake ; γ–Aminobutyric acid</subject><ispartof>Journal of neurochemistry, 1988-04, Vol.50 (4), p.1237-1242</ispartof><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4307-637deef0ef89a5fbb1dc9a8a02bb8beba55c02bb9215d51d10f464aebbfedd043</citedby><cites>FETCH-LOGICAL-c4307-637deef0ef89a5fbb1dc9a8a02bb8beba55c02bb9215d51d10f464aebbfedd043</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1471-4159.1988.tb10599.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1471-4159.1988.tb10599.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6975962$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2964510$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fykse, Else M.</creatorcontrib><creatorcontrib>Fonnum, Frode</creatorcontrib><title>Uptake of γ‐Aminobutyric Acid by a Synaptic Vesicle Fraction Isolated from Rat Brain</title><title>Journal of neurochemistry</title><addtitle>J Neurochem</addtitle><description>: γ‐Aminobutyric acid (GABA) was taken up by a MgATP‐dependent mechanism into synaptic vesicles isolated by hypoosmotic shock and density gradient centrifugation. The properties of the vesicular uptake differed clearly from those of synaptosomal and glial uptake, both with respect to Na+, Mg2+, and ATP dependence and with respect to response to general GABA uptake inhibitors such as nipecotic acid, diaminobutyric acid, and β‐alanine. The uptake showed a Km of 5.6 mM and a net uptake rate of 1,500 pmol/min/mg of protein. It is suggested that the vesicular uptake of GABA is driven by an electrochemical proton gradient generated by a Mg2+‐ATPase.</description><subject>Adenosine Triphosphate - pharmacology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Brain - ultrastructure</subject><subject>Ca(2+) Mg(2+)-ATPase - metabolism</subject><subject>Carbonyl Cyanide m-Chlorophenyl Hydrazone - pharmacology</subject><subject>Cell physiology</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Electrochemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gamma-Aminobutyric Acid - metabolism</subject><subject>Kinetics</subject><subject>Magnesium - pharmacology</subject><subject>Male</subject><subject>Molecular and cellular biology</subject><subject>Neurotransmission</subject><subject>Osmotic Pressure</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Sodium - pharmacology</subject><subject>Synaptic vesicles</subject><subject>Synaptic Vesicles - metabolism</subject><subject>Synaptosomes</subject><subject>Synaptosomes - metabolism</subject><subject>γ‐Aminobutyric acid uptake</subject><subject>γ–Aminobutyric acid</subject><issn>0022-3042</issn><issn>1471-4159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkE1u1DAUxy0EKkPhCEgWQuwSbMdOYjZoGFFoVbUSUFhaz1-Sh3xM7Yxodhyhd-EeHIKTkGii2SK8eZb_v_f89EPoBSU5nc7rbU55RTNOhcyprOt80JQIKfO7B2h1jB6iFSGMZQXh7DF6ktKWEFrykp6gEyZLLihZoW83uwG-O9x7_PvXn5_36zZ0vd4PYwwGr02wWI8Y8Oexg90wPX11KZjG4bMIZgh9h89T38DgLPaxb_EnGPC7CKF7ih55aJJ7ttRTdHP2_svmY3Z5_eF8s77MDC9IlZVFZZ3zxPlagvBaU2sk1ECY1rV2GoQw810yKqyglhLPSw5Oa--sJbw4Ra8Oc3exv927NKg2JOOaBjrX75OqasomM_8GKZesKriYwDcH0MQ-pei82sXQQhwVJWrWr7Zqdqxmx2rWrxb96m5qfr78stets8fWxfeUv1xySAYaH6EzIR2xUlZClmzC3h6wH6Fx438soC6uNpQVVfEX0TukXQ</recordid><startdate>198804</startdate><enddate>198804</enddate><creator>Fykse, Else M.</creator><creator>Fonnum, Frode</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>198804</creationdate><title>Uptake of γ‐Aminobutyric Acid by a Synaptic Vesicle Fraction Isolated from Rat Brain</title><author>Fykse, Else M. ; Fonnum, Frode</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4307-637deef0ef89a5fbb1dc9a8a02bb8beba55c02bb9215d51d10f464aebbfedd043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Adenosine Triphosphate - pharmacology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Brain - ultrastructure</topic><topic>Ca(2+) Mg(2+)-ATPase - metabolism</topic><topic>Carbonyl Cyanide m-Chlorophenyl Hydrazone - pharmacology</topic><topic>Cell physiology</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Electrochemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gamma-Aminobutyric Acid - metabolism</topic><topic>Kinetics</topic><topic>Magnesium - pharmacology</topic><topic>Male</topic><topic>Molecular and cellular biology</topic><topic>Neurotransmission</topic><topic>Osmotic Pressure</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Sodium - pharmacology</topic><topic>Synaptic vesicles</topic><topic>Synaptic Vesicles - metabolism</topic><topic>Synaptosomes</topic><topic>Synaptosomes - metabolism</topic><topic>γ‐Aminobutyric acid uptake</topic><topic>γ–Aminobutyric acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fykse, Else M.</creatorcontrib><creatorcontrib>Fonnum, Frode</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fykse, Else M.</au><au>Fonnum, Frode</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Uptake of γ‐Aminobutyric Acid by a Synaptic Vesicle Fraction Isolated from Rat Brain</atitle><jtitle>Journal of neurochemistry</jtitle><addtitle>J Neurochem</addtitle><date>1988-04</date><risdate>1988</risdate><volume>50</volume><issue>4</issue><spage>1237</spage><epage>1242</epage><pages>1237-1242</pages><issn>0022-3042</issn><eissn>1471-4159</eissn><coden>JONRA9</coden><abstract>: γ‐Aminobutyric acid (GABA) was taken up by a MgATP‐dependent mechanism into synaptic vesicles isolated by hypoosmotic shock and density gradient centrifugation. The properties of the vesicular uptake differed clearly from those of synaptosomal and glial uptake, both with respect to Na+, Mg2+, and ATP dependence and with respect to response to general GABA uptake inhibitors such as nipecotic acid, diaminobutyric acid, and β‐alanine. The uptake showed a Km of 5.6 mM and a net uptake rate of 1,500 pmol/min/mg of protein. It is suggested that the vesicular uptake of GABA is driven by an electrochemical proton gradient generated by a Mg2+‐ATPase.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>2964510</pmid><doi>10.1111/j.1471-4159.1988.tb10599.x</doi><tpages>6</tpages></addata></record> |
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| ispartof | Journal of neurochemistry, 1988-04, Vol.50 (4), p.1237-1242 |
| issn | 0022-3042 1471-4159 |
| language | eng |
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| source | MEDLINE; Access via Wiley Online Library |
| subjects | Adenosine Triphosphate - pharmacology Animals Biological and medical sciences Brain - ultrastructure Ca(2+) Mg(2+)-ATPase - metabolism Carbonyl Cyanide m-Chlorophenyl Hydrazone - pharmacology Cell physiology Chromatography, High Pressure Liquid Electrochemistry Fundamental and applied biological sciences. Psychology gamma-Aminobutyric Acid - metabolism Kinetics Magnesium - pharmacology Male Molecular and cellular biology Neurotransmission Osmotic Pressure Rats Rats, Inbred Strains Sodium - pharmacology Synaptic vesicles Synaptic Vesicles - metabolism Synaptosomes Synaptosomes - metabolism γ‐Aminobutyric acid uptake γ–Aminobutyric acid |
| title | Uptake of γ‐Aminobutyric Acid by a Synaptic Vesicle Fraction Isolated from Rat Brain |
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