Immunogenicity and epitope mapping of foreign sequences via genetically engineered filamentous phage
Repeat regions of the circumsporozoite protein gene of Plasmodium falciparum were cloned into the pIII gene of a filamentous phage. These genetically engineered filamentous phage display the recombinant proteins on their surface. We demonstrate that they are both antigenic and immunogenic in rabbits...
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| Veröffentlicht in: | The Journal of biological chemistry 1988-03, Vol.263 (9), p.4318-4322 |
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| container_end_page | 4322 |
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| container_issue | 9 |
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| container_title | The Journal of biological chemistry |
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| creator | de la Cruz, V F Lal, A A McCutchan, T F |
| description | Repeat regions of the circumsporozoite protein gene of Plasmodium falciparum were cloned into the pIII gene of a filamentous phage. These genetically engineered filamentous phage display the recombinant proteins on their surface. We demonstrate that they are both antigenic and immunogenic in rabbits. The recombinant phage were shown to be useful as a source of antigen for this scarce malaria protein, for producing carrier-hapten conjugates for obtaining immunological reagents in rabbits, and for B epitope mapping. In addition, in mice the antibody response to the cloned antigens seems to be controlled by immune response genes. Therefore this system also has the potential for use in helper T cell epitope mapping using inbred mouse strains. This advantage will be of use in vaccine development. |
| doi_str_mv | 10.1016/S0021-9258(18)68927-6 |
| format | Article |
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Technologies ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Microscopy, Electron ; Molecular and cellular biology ; Molecular cloning ; Plasmodium falciparum ; Plasmodium falciparum - genetics ; Rabbits ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - immunology ; Recombinant Proteins - immunology ; Repetitive Sequences, Nucleic Acid</subject><ispartof>The Journal of biological chemistry, 1988-03, Vol.263 (9), p.4318-4322</ispartof><rights>1988 © 1988 ASBMB. 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These genetically engineered filamentous phage display the recombinant proteins on their surface. We demonstrate that they are both antigenic and immunogenic in rabbits. The recombinant phage were shown to be useful as a source of antigen for this scarce malaria protein, for producing carrier-hapten conjugates for obtaining immunological reagents in rabbits, and for B epitope mapping. In addition, in mice the antibody response to the cloned antigens seems to be controlled by immune response genes. Therefore this system also has the potential for use in helper T cell epitope mapping using inbred mouse strains. This advantage will be of use in vaccine development.</description><subject>Animals</subject><subject>Antibody Formation</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cell coat. Cell surface</subject><subject>Cell structures and functions</subject><subject>Cloning, Molecular</subject><subject>Coliphages - genetics</subject><subject>Coliphages - immunology</subject><subject>Epitopes - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Methods. Procedures. Technologies</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred C57BL</subject><subject>Microscopy, Electron</subject><subject>Molecular and cellular biology</subject><subject>Molecular cloning</subject><subject>Plasmodium falciparum</subject><subject>Plasmodium falciparum - genetics</subject><subject>Rabbits</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - immunology</subject><subject>Recombinant Proteins - immunology</subject><subject>Repetitive Sequences, Nucleic Acid</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUuLFDEUhYMoYzv6EwaCiOiiNI9KKlkNMvgYGHChgruQSm6qI1WpMqke6X9verrp7dxNFvc7uYdzELqi5AMlVH78QQijjWZCvaPqvVSadY18gjaUKN5wQX8_RZsz8hy9KOUPqdNqeoEuWCsI0XSD_O007dI8QIourntsk8ewxHVeAE92WWIa8BxwmDPEIeECf3eQHBR8Hy2uKlijs-O4x5CGmAAyeBziaCdI67wreNnaAV6iZ8GOBV6d3kv068vnnzffmrvvX29vPt01TlK2NpwF6ZXvaeeZFr4NUthAdNcRYVXQVoHoe-Go7zUnlhEWgpLcasnblgth-SV6e_x3yXP1WVYzxeJgHG2CasZ0ijKqJXsUpK0mbYUrKI6gy3MpGYJZcpxs3htKzKEG81CDOWRsqDIPNRhZdVenA7t-An9WnXKv-zenvS01v5BtcrGcsa7rWk50xV4fsW0ctv9iBtPH2W1hMkxyo03LqarQ9RGCGu19hGyKi4eSfBW41fg5PuL2P-2msJw</recordid><startdate>19880325</startdate><enddate>19880325</enddate><creator>de la Cruz, V F</creator><creator>Lal, A A</creator><creator>McCutchan, T F</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>19880325</creationdate><title>Immunogenicity and epitope mapping of foreign sequences via genetically engineered filamentous phage</title><author>de la Cruz, V F ; Lal, A A ; McCutchan, T F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c612t-32f6d8db17d295d4f65af097705a8f9a8e5bb5c1db930a202ff863a96344355a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Animals</topic><topic>Antibody Formation</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cell coat. Cell surface</topic><topic>Cell structures and functions</topic><topic>Cloning, Molecular</topic><topic>Coliphages - genetics</topic><topic>Coliphages - immunology</topic><topic>Epitopes - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Methods. Procedures. 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| fulltext | fulltext |
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| ispartof | The Journal of biological chemistry, 1988-03, Vol.263 (9), p.4318-4322 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78121962 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Antibody Formation Biological and medical sciences Biotechnology Cell coat. Cell surface Cell structures and functions Cloning, Molecular Coliphages - genetics Coliphages - immunology Epitopes - analysis Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Methods. Procedures. Technologies Mice Mice, Inbred BALB C Mice, Inbred C57BL Microscopy, Electron Molecular and cellular biology Molecular cloning Plasmodium falciparum Plasmodium falciparum - genetics Rabbits Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - immunology Recombinant Proteins - immunology Repetitive Sequences, Nucleic Acid |
| title | Immunogenicity and epitope mapping of foreign sequences via genetically engineered filamentous phage |
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