Identification of bldA mutants of Streptomyces griseus

Identification of bldA mutants of Streptomyces griseus

The bldA gene (encoding tRNA UUA Leu) from Streptomyces griseus (Sg) was cloned by hybridization with bldA from Streptomyces coelicolor (Sc). Introduction of Sg bldA into Sc bldA mutants restored sporulation and actinorhodin production. Sporulation of a subset of Sg bald mutants, which produce no ae...

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Veröffentlicht in:Gene 1996-05, Vol.171 (1), p.75-78
Hauptverfasser: Kwak, Jangyul, McCue, Lee Ann, Kendrick, Kathleen E.
Format: Artikel
Sprache:eng
Schlagworte:
Anthraquinones - metabolism
Base Sequence
Cloning, Molecular
Conserved Sequence - genetics
Differentiation
Genetic Complementation Test
Molecular Sequence Data
Nucleic Acid Conformation
Point Mutation - genetics
Promoter Regions, Genetic - genetics
RNA, Transfer, Leu - chemistry
RNA, Transfer, Leu - genetics
Spores, Bacterial - genetics
sporulation
Streptomyces - genetics
Streptomyces griseus
Streptomyces griseus - genetics
Suppression, Genetic
tRNA
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Zusammenfassung:The bldA gene (encoding tRNA UUA Leu) from Streptomyces griseus (Sg) was cloned by hybridization with bldA from Streptomyces coelicolor (Sc). Introduction of Sg bldA into Sc bldA mutants restored sporulation and actinorhodin production. Sporulation of a subset of Sg bald mutants, which produce no aerial mycelium or spores, was restored in the presence of bldA from Sc or Sg. The nucleotide sequences of the bldA alleles from two such bald mutants revealed point mutations in the anticodon stem and the TΨC stem.
ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(96)00066-2