Isolation of varicella-zoster virus from vesicles in children with varicella
Varicella‐zoster virus (VZV) was isolated from 29 samples of the vesicular fluid in 13 otherwise healthy children with varicella who were aged from 7 months to 7 years. Human embryonic lung cells were used for viral isolation, and VZV was identified by a characteristic cytopathic effect and an indir...
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| Veröffentlicht in: | Journal of medical virology 1996-04, Vol.48 (4), p.326-328 |
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| creator | Ozaki, Takao Kajita, Yuji Namazue, Junko Yamanishi, Koichi |
| description | Varicella‐zoster virus (VZV) was isolated from 29 samples of the vesicular fluid in 13 otherwise healthy children with varicella who were aged from 7 months to 7 years. Human embryonic lung cells were used for viral isolation, and VZV was identified by a characteristic cytopathic effect and an indirect immunofluorescence assay. VZV was found in 17 samples; in two (12%) of which it was also detected after filtration (0.45 μm). The rate of isolation was 100% in the first two days after the onset of the disease. It declined gradually with time to 1 of 6 in the samples 6 days after the clinical onset. Specific IgG antibody to VZV was investigated in the same materials. The positive rate was 0% (0/13) in the first 3 days and increased to 7 of 16 in the following 3 days after the onset. VZV was not isolated from samples with specific antibody. In conclusion, VZV can be isolated easily from vesicles within the first 3 days of onset, but the filtration of samples affects its isolation. Infective VZV disappears gradually in vesicles after the first 3 days, and this may be related to the establishment of immune reactions including specific antibody. © 1996 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/(SICI)1096-9071(199604)48:4<326::AID-JMV5>3.0.CO;2-9 |
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Human embryonic lung cells were used for viral isolation, and VZV was identified by a characteristic cytopathic effect and an indirect immunofluorescence assay. VZV was found in 17 samples; in two (12%) of which it was also detected after filtration (0.45 μm). The rate of isolation was 100% in the first two days after the onset of the disease. It declined gradually with time to 1 of 6 in the samples 6 days after the clinical onset. Specific IgG antibody to VZV was investigated in the same materials. The positive rate was 0% (0/13) in the first 3 days and increased to 7 of 16 in the following 3 days after the onset. VZV was not isolated from samples with specific antibody. In conclusion, VZV can be isolated easily from vesicles within the first 3 days of onset, but the filtration of samples affects its isolation. Infective VZV disappears gradually in vesicles after the first 3 days, and this may be related to the establishment of immune reactions including specific antibody. © 1996 Wiley‐Liss, Inc.</description><identifier>ISSN: 0146-6615</identifier><identifier>EISSN: 1096-9071</identifier><identifier>DOI: 10.1002/(SICI)1096-9071(199604)48:4<326::AID-JMV5>3.0.CO;2-9</identifier><identifier>PMID: 8699164</identifier><identifier>CODEN: JMVIDB</identifier><language>eng</language><publisher>New York: John Wiley & Sons, Inc</publisher><subject>Antibodies, Viral - analysis ; Biological and medical sciences ; Blister - immunology ; Blister - virology ; Cells, Cultured ; Chickenpox - immunology ; Chickenpox - virology ; Child ; Child, Preschool ; Female ; Fundamental and applied biological sciences. Psychology ; Herpesvirus 3, Human - immunology ; Herpesvirus 3, Human - isolation & purification ; Humans ; Immunoglobulin G - analysis ; Infant ; Male ; Microbiology ; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains ; varicella-zoster virus ; vesicle ; viral isolation ; Virology</subject><ispartof>Journal of medical virology, 1996-04, Vol.48 (4), p.326-328</ispartof><rights>Copyright © 1996 Wiley‐Liss, Inc.</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c4585-82cbced1844971c67e083e2d439850109582f2a86d4a0c03e313276e60bd5b1b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F%28SICI%291096-9071%28199604%2948%3A4%3C326%3A%3AAID-JMV5%3E3.0.CO%3B2-9$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F%28SICI%291096-9071%28199604%2948%3A4%3C326%3A%3AAID-JMV5%3E3.0.CO%3B2-9$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3048948$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8699164$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ozaki, Takao</creatorcontrib><creatorcontrib>Kajita, Yuji</creatorcontrib><creatorcontrib>Namazue, Junko</creatorcontrib><creatorcontrib>Yamanishi, Koichi</creatorcontrib><title>Isolation of varicella-zoster virus from vesicles in children with varicella</title><title>Journal of medical virology</title><addtitle>J. Med. Virol</addtitle><description>Varicella‐zoster virus (VZV) was isolated from 29 samples of the vesicular fluid in 13 otherwise healthy children with varicella who were aged from 7 months to 7 years. Human embryonic lung cells were used for viral isolation, and VZV was identified by a characteristic cytopathic effect and an indirect immunofluorescence assay. VZV was found in 17 samples; in two (12%) of which it was also detected after filtration (0.45 μm). The rate of isolation was 100% in the first two days after the onset of the disease. It declined gradually with time to 1 of 6 in the samples 6 days after the clinical onset. Specific IgG antibody to VZV was investigated in the same materials. The positive rate was 0% (0/13) in the first 3 days and increased to 7 of 16 in the following 3 days after the onset. VZV was not isolated from samples with specific antibody. In conclusion, VZV can be isolated easily from vesicles within the first 3 days of onset, but the filtration of samples affects its isolation. Infective VZV disappears gradually in vesicles after the first 3 days, and this may be related to the establishment of immune reactions including specific antibody. © 1996 Wiley‐Liss, Inc.</description><subject>Antibodies, Viral - analysis</subject><subject>Biological and medical sciences</subject><subject>Blister - immunology</subject><subject>Blister - virology</subject><subject>Cells, Cultured</subject><subject>Chickenpox - immunology</subject><subject>Chickenpox - virology</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Herpesvirus 3, Human - immunology</subject><subject>Herpesvirus 3, Human - isolation & purification</subject><subject>Humans</subject><subject>Immunoglobulin G - analysis</subject><subject>Infant</subject><subject>Male</subject><subject>Microbiology</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>varicella-zoster virus</subject><subject>vesicle</subject><subject>viral isolation</subject><subject>Virology</subject><issn>0146-6615</issn><issn>1096-9071</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV1v0zAUhiMEGmXwE5BygdB2kXL8EccuE9IWYGRqqRADJG6OHMfRPNJks9uO8etJ1qq7AGlXluX3PH51nig6IjAmAPTNwdciLw4JKJEoyMgBUUoAP-Rywo8YFZPJcfE-OZt9T9-xMYzz-VuaqEfRaDfwOBoB4SIRgqRPo2chXAKAVJTuRXtSKEUEH0XTInSNXrqujbs6XmvvjG0anfzpwtL6eO38KsS17xbx2gZnGhti18bmwjWVt21845YX91PPoye1boJ9sT33o28fP5znn5Lp_LTIj6eJ4alME0lNaWxFJOcqI0ZkFiSztOJMyRT6_qmkNdVSVFyDAWYZYTQTVkBZpSUp2X70esO98t31yoYlLly4a9DabhUwk4T0MPFgkGQgKM9IHzzfBI3vQvC2xivvFtrfIgEcbCAONnBYLg7LxY0N5BI59jYQexs42ECGgPkcKaoe-3L7_6pc2GoH3a6_f3-1fdfB6Kb2ujUu7GIMuFRc3re7cY29_afaA83-U-zu3mOTDdb1rn_vsNr_QpGxLMUfn0_xizqZ_YSzGTL2F8QXv1k</recordid><startdate>199604</startdate><enddate>199604</enddate><creator>Ozaki, Takao</creator><creator>Kajita, Yuji</creator><creator>Namazue, Junko</creator><creator>Yamanishi, Koichi</creator><general>John Wiley & Sons, Inc</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>199604</creationdate><title>Isolation of varicella-zoster virus from vesicles in children with varicella</title><author>Ozaki, Takao ; Kajita, Yuji ; Namazue, Junko ; Yamanishi, Koichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4585-82cbced1844971c67e083e2d439850109582f2a86d4a0c03e313276e60bd5b1b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Antibodies, Viral - analysis</topic><topic>Biological and medical sciences</topic><topic>Blister - immunology</topic><topic>Blister - virology</topic><topic>Cells, Cultured</topic><topic>Chickenpox - immunology</topic><topic>Chickenpox - virology</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Herpesvirus 3, Human - immunology</topic><topic>Herpesvirus 3, Human - isolation & purification</topic><topic>Humans</topic><topic>Immunoglobulin G - analysis</topic><topic>Infant</topic><topic>Male</topic><topic>Microbiology</topic><topic>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</topic><topic>varicella-zoster virus</topic><topic>vesicle</topic><topic>viral isolation</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ozaki, Takao</creatorcontrib><creatorcontrib>Kajita, Yuji</creatorcontrib><creatorcontrib>Namazue, Junko</creatorcontrib><creatorcontrib>Yamanishi, Koichi</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ozaki, Takao</au><au>Kajita, Yuji</au><au>Namazue, Junko</au><au>Yamanishi, Koichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of varicella-zoster virus from vesicles in children with varicella</atitle><jtitle>Journal of medical virology</jtitle><addtitle>J. Med. Virol</addtitle><date>1996-04</date><risdate>1996</risdate><volume>48</volume><issue>4</issue><spage>326</spage><epage>328</epage><pages>326-328</pages><issn>0146-6615</issn><eissn>1096-9071</eissn><coden>JMVIDB</coden><abstract>Varicella‐zoster virus (VZV) was isolated from 29 samples of the vesicular fluid in 13 otherwise healthy children with varicella who were aged from 7 months to 7 years. Human embryonic lung cells were used for viral isolation, and VZV was identified by a characteristic cytopathic effect and an indirect immunofluorescence assay. VZV was found in 17 samples; in two (12%) of which it was also detected after filtration (0.45 μm). The rate of isolation was 100% in the first two days after the onset of the disease. It declined gradually with time to 1 of 6 in the samples 6 days after the clinical onset. Specific IgG antibody to VZV was investigated in the same materials. The positive rate was 0% (0/13) in the first 3 days and increased to 7 of 16 in the following 3 days after the onset. VZV was not isolated from samples with specific antibody. In conclusion, VZV can be isolated easily from vesicles within the first 3 days of onset, but the filtration of samples affects its isolation. Infective VZV disappears gradually in vesicles after the first 3 days, and this may be related to the establishment of immune reactions including specific antibody. © 1996 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>John Wiley & Sons, Inc</pub><pmid>8699164</pmid><doi>10.1002/(SICI)1096-9071(199604)48:4<326::AID-JMV5>3.0.CO;2-9</doi><tpages>3</tpages></addata></record> |
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| subjects | Antibodies, Viral - analysis Biological and medical sciences Blister - immunology Blister - virology Cells, Cultured Chickenpox - immunology Chickenpox - virology Child Child, Preschool Female Fundamental and applied biological sciences. Psychology Herpesvirus 3, Human - immunology Herpesvirus 3, Human - isolation & purification Humans Immunoglobulin G - analysis Infant Male Microbiology Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains varicella-zoster virus vesicle viral isolation Virology |
| title | Isolation of varicella-zoster virus from vesicles in children with varicella |
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