Involvement of GABA systems in acetylcholine release induced by 5-HT3 receptor blockade in slices from rat entorhinal cortex
The aim of the present study was to examine the role of 5-HT3 receptors in spontaneous and K(+)-evoked acetylcholine (ACh) release from rat entorhinal cortex and striatal slices. The 5-HT3 receptor antagonists ondansetron and granisetron (0.01-10 microM) produced a concentration-dependent increase i...
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| Veröffentlicht in: | Brain research 1996-03, Vol.712 (2), p.274-280 |
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| creator | RAMIREZ, M. J CENARRUZABEITIA, E LASHERAS, B DEL RIO, J |
| description | The aim of the present study was to examine the role of 5-HT3 receptors in spontaneous and K(+)-evoked acetylcholine (ACh) release from rat entorhinal cortex and striatal slices. The 5-HT3 receptor antagonists ondansetron and granisetron (0.01-10 microM) produced a concentration-dependent increase in both spontaneous and K(+)-evoked [3H]ACh release in the two brain regions studied. The release of ACh was Ca(2+)-dependent and tetrodotoxin-sensitive. 5-HT3 receptor agonists, such as 2-methyl-5-HT and 1-phenylbiguanide, at concentrations up to 1 microM, did not show any intrinsic effect on [3H]ACh release in both rat brain regions. However, 2-methyl-5-HT, 1 microM, fully blocked the ondansetron-induced enhancement in both basal and K(+)-evoked ACh release, suggesting that 5-HT, through 5-HT3 receptor activation, tonically inhibits ACh release. The possible implication of interposed inhibitory systems in ACh release after 5-HT3 receptor blockade was subsequently analyzed. While the effect of ondansetron was not modified by haloperidol or naloxone, the GABAA receptor antagonist bicuculline produced a marked potentiation of ACh release in the entorhinal cortex but not in the striatum. The results suggest that in this cortical area 5-HT activates 5-HT3 receptors located on GABAergic neurons which in turn inhibit cholinergic function. |
| doi_str_mv | 10.1016/0006-8993(95)01471-3 |
| format | Article |
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J ; CENARRUZABEITIA, E ; LASHERAS, B ; DEL RIO, J</creator><creatorcontrib>RAMIREZ, M. J ; CENARRUZABEITIA, E ; LASHERAS, B ; DEL RIO, J</creatorcontrib><description>The aim of the present study was to examine the role of 5-HT3 receptors in spontaneous and K(+)-evoked acetylcholine (ACh) release from rat entorhinal cortex and striatal slices. The 5-HT3 receptor antagonists ondansetron and granisetron (0.01-10 microM) produced a concentration-dependent increase in both spontaneous and K(+)-evoked [3H]ACh release in the two brain regions studied. The release of ACh was Ca(2+)-dependent and tetrodotoxin-sensitive. 5-HT3 receptor agonists, such as 2-methyl-5-HT and 1-phenylbiguanide, at concentrations up to 1 microM, did not show any intrinsic effect on [3H]ACh release in both rat brain regions. However, 2-methyl-5-HT, 1 microM, fully blocked the ondansetron-induced enhancement in both basal and K(+)-evoked ACh release, suggesting that 5-HT, through 5-HT3 receptor activation, tonically inhibits ACh release. The possible implication of interposed inhibitory systems in ACh release after 5-HT3 receptor blockade was subsequently analyzed. While the effect of ondansetron was not modified by haloperidol or naloxone, the GABAA receptor antagonist bicuculline produced a marked potentiation of ACh release in the entorhinal cortex but not in the striatum. The results suggest that in this cortical area 5-HT activates 5-HT3 receptors located on GABAergic neurons which in turn inhibit cholinergic function.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/0006-8993(95)01471-3</identifier><identifier>PMID: 8814902</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier</publisher><subject>Acetylcholine - metabolism ; Animals ; Biological and medical sciences ; Central nervous system ; Central neurotransmission. Neuromudulation. Pathways and receptors ; Cerebral Cortex - drug effects ; Cerebral Cortex - metabolism ; Cholinergic Agents - pharmacology ; Entorhinal Cortex - drug effects ; Entorhinal Cortex - metabolism ; Fundamental and applied biological sciences. Psychology ; gamma-Aminobutyric Acid - physiology ; In Vitro Techniques ; Male ; Neostriatum - drug effects ; Neostriatum - metabolism ; Potassium - pharmacology ; Rats ; Rats, Wistar ; Receptors, Serotonin - drug effects ; Receptors, Serotonin - metabolism ; Serotonin Antagonists - pharmacology ; Vertebrates: nervous system and sense organs</subject><ispartof>Brain research, 1996-03, Vol.712 (2), p.274-280</ispartof><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c331t-5d50669175b9c0668ac0ca2d018694baf9a7c406617e7dac192ece7ad89ac55f3</citedby><cites>FETCH-LOGICAL-c331t-5d50669175b9c0668ac0ca2d018694baf9a7c406617e7dac192ece7ad89ac55f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3030452$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8814902$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>RAMIREZ, M. J</creatorcontrib><creatorcontrib>CENARRUZABEITIA, E</creatorcontrib><creatorcontrib>LASHERAS, B</creatorcontrib><creatorcontrib>DEL RIO, J</creatorcontrib><title>Involvement of GABA systems in acetylcholine release induced by 5-HT3 receptor blockade in slices from rat entorhinal cortex</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>The aim of the present study was to examine the role of 5-HT3 receptors in spontaneous and K(+)-evoked acetylcholine (ACh) release from rat entorhinal cortex and striatal slices. The 5-HT3 receptor antagonists ondansetron and granisetron (0.01-10 microM) produced a concentration-dependent increase in both spontaneous and K(+)-evoked [3H]ACh release in the two brain regions studied. The release of ACh was Ca(2+)-dependent and tetrodotoxin-sensitive. 5-HT3 receptor agonists, such as 2-methyl-5-HT and 1-phenylbiguanide, at concentrations up to 1 microM, did not show any intrinsic effect on [3H]ACh release in both rat brain regions. However, 2-methyl-5-HT, 1 microM, fully blocked the ondansetron-induced enhancement in both basal and K(+)-evoked ACh release, suggesting that 5-HT, through 5-HT3 receptor activation, tonically inhibits ACh release. The possible implication of interposed inhibitory systems in ACh release after 5-HT3 receptor blockade was subsequently analyzed. While the effect of ondansetron was not modified by haloperidol or naloxone, the GABAA receptor antagonist bicuculline produced a marked potentiation of ACh release in the entorhinal cortex but not in the striatum. The results suggest that in this cortical area 5-HT activates 5-HT3 receptors located on GABAergic neurons which in turn inhibit cholinergic function.</description><subject>Acetylcholine - metabolism</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Central nervous system</subject><subject>Central neurotransmission. Neuromudulation. Pathways and receptors</subject><subject>Cerebral Cortex - drug effects</subject><subject>Cerebral Cortex - metabolism</subject><subject>Cholinergic Agents - pharmacology</subject><subject>Entorhinal Cortex - drug effects</subject><subject>Entorhinal Cortex - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gamma-Aminobutyric Acid - physiology</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Neostriatum - drug effects</subject><subject>Neostriatum - metabolism</subject><subject>Potassium - pharmacology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Receptors, Serotonin - drug effects</subject><subject>Receptors, Serotonin - metabolism</subject><subject>Serotonin Antagonists - pharmacology</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kMFq3DAQhkVoSDdp3qAFHUppDk4ly7Kt4ya0m0Agl-QsxuMxcStbW8leupCHr5wsexoN_zcz4mPssxTXUsjyhxCizGpj1Hejr4QsKpmpE7aSdZVnZV6ID2x1RD6y8xh_p1YpI87YWV3Lwoh8xV7vx513OxponLjv-GZ9s-ZxHycaIu9HDkjT3uGLd_1IPJAjiJSCdkZqebPnOrt7UilA2k4-8MZ5_APtgvDoeqTIu-AHHmDi6YQPL_0IjqMPE_37xE47cJEuD_WCPf_6-XR7lz08bu5v1w8ZKiWnTLdalKWRlW4MplcNKBDyVsi6NEUDnYEKixTIiqoWUJo8faeCtjaAWnfqgn1737sN_u9McbJDH5Gcg5H8HG1Vy8WoTGDxDmLwMQbq7Db0A4S9lcIuiF2M2sWoNdq-SbcqjX057J-bgdrj0MFyyr8ecogIrgswYh-PmBJKFDpX_wGCtoqL</recordid><startdate>19960318</startdate><enddate>19960318</enddate><creator>RAMIREZ, M. 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J ; CENARRUZABEITIA, E ; LASHERAS, B ; DEL RIO, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c331t-5d50669175b9c0668ac0ca2d018694baf9a7c406617e7dac192ece7ad89ac55f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Acetylcholine - metabolism</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Central nervous system</topic><topic>Central neurotransmission. Neuromudulation. Pathways and receptors</topic><topic>Cerebral Cortex - drug effects</topic><topic>Cerebral Cortex - metabolism</topic><topic>Cholinergic Agents - pharmacology</topic><topic>Entorhinal Cortex - drug effects</topic><topic>Entorhinal Cortex - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gamma-Aminobutyric Acid - physiology</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Neostriatum - drug effects</topic><topic>Neostriatum - metabolism</topic><topic>Potassium - pharmacology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Receptors, Serotonin - drug effects</topic><topic>Receptors, Serotonin - metabolism</topic><topic>Serotonin Antagonists - pharmacology</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>RAMIREZ, M. 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The release of ACh was Ca(2+)-dependent and tetrodotoxin-sensitive. 5-HT3 receptor agonists, such as 2-methyl-5-HT and 1-phenylbiguanide, at concentrations up to 1 microM, did not show any intrinsic effect on [3H]ACh release in both rat brain regions. However, 2-methyl-5-HT, 1 microM, fully blocked the ondansetron-induced enhancement in both basal and K(+)-evoked ACh release, suggesting that 5-HT, through 5-HT3 receptor activation, tonically inhibits ACh release. The possible implication of interposed inhibitory systems in ACh release after 5-HT3 receptor blockade was subsequently analyzed. While the effect of ondansetron was not modified by haloperidol or naloxone, the GABAA receptor antagonist bicuculline produced a marked potentiation of ACh release in the entorhinal cortex but not in the striatum. 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| subjects | Acetylcholine - metabolism Animals Biological and medical sciences Central nervous system Central neurotransmission. Neuromudulation. Pathways and receptors Cerebral Cortex - drug effects Cerebral Cortex - metabolism Cholinergic Agents - pharmacology Entorhinal Cortex - drug effects Entorhinal Cortex - metabolism Fundamental and applied biological sciences. Psychology gamma-Aminobutyric Acid - physiology In Vitro Techniques Male Neostriatum - drug effects Neostriatum - metabolism Potassium - pharmacology Rats Rats, Wistar Receptors, Serotonin - drug effects Receptors, Serotonin - metabolism Serotonin Antagonists - pharmacology Vertebrates: nervous system and sense organs |
| title | Involvement of GABA systems in acetylcholine release induced by 5-HT3 receptor blockade in slices from rat entorhinal cortex |
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