AN IMMUNOHISTOCHEMICAL STUDY OF TAL-1 PROTEIN EXPRESSION IN LEUKAEMIAS AND LYMPHOMAS WITH A NOVEL MONOCLONAL ANTIBODY, 2TL 242
Fifty formalin fixed, paraffin‐embedded cases of T‐acute lymphoblastic leukaemia (T‐ALL) from 12 bone marrow trephines and 38 lymph nodes were stained with a new monoclonal antibody, 2TL 242, raised against recombinant TAL1 protein. The antibody recognizes TAL‐1 polypeptides of molecular weight 39 a...
Gespeichert in:
| Veröffentlicht in: | The Journal of pathology 1996-03, Vol.178 (3), p.311-315 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 315 |
|---|---|
| container_issue | 3 |
| container_start_page | 311 |
| container_title | The Journal of pathology |
| container_volume | 178 |
| creator | CHETTY, RUNJAN PULFORD, KAREN JONES, MARGARET MATHIEU-MAHUL, DANIELE CLOSE, PAULINE HUSSEIN, SONAY PALLESEN, GORM RALFKIAER, ELIZABETH STEIN, HARALD GATTER, KEVIN MASON, DAVID |
| description | Fifty formalin fixed, paraffin‐embedded cases of T‐acute lymphoblastic leukaemia (T‐ALL) from 12 bone marrow trephines and 38 lymph nodes were stained with a new monoclonal antibody, 2TL 242, raised against recombinant TAL1 protein. The antibody recognizes TAL‐1 polypeptides of molecular weight 39 and 41 kD (full length). In addition, a variety of other leukaemias and lymphomas were also stained with 2TL 242. Twenty‐four of the 50 cases of T‐ALL showed nuclear positivity, ranging from 10 to 90 per cent of leukaemic cells. A positive staining reaction was nuclear and stippled in pattern. Nuclear staining was not seen in any other type of leukaemia or lymphoma. Five cases of follicular lymphoma showed diffuse cytoplasmic staining of variable intensity. Although some background staining is obtained with this antibody, positive nuclear staining is easily distinguishable. This monoclonal antibody has a potential role in primary diagnosis and in the detection of minimal residual disease in T‐ALL. |
| doi_str_mv | 10.1002/(SICI)1096-9896(199603)178:3<311::AID-PATH477>3.0.CO;2-B |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_78105967</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>78105967</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4407-f691a9a8e70dc80195a31ce4a06d48bfda93339fff9fa38202a5acdc493614c83</originalsourceid><addsrcrecordid>eNqFkdFu0zAUhiMEGmXwCEi-QGiTlmLHSRx3CJGmWRNI4mpJ2SoujrzUkQLtusWrYDc8O45aegMSV1Hs_3z-dT7L-kjwkGDsvDsp0yg9JZj7Ng-4f0I49zE9JSwY0feUkNEoTCf2LKwSl7EPdIiHkTh37PETa3AYemoNDMqxqUvYc-uF1t8wxpx73pF1FDAWUMoG1q-wQGmezwuRpGUloiTO0yjMUFnNJwskLlAVZjZBs0tRxWmB4uvZZVyWqTBTBcri-efQDIQlCosJyhb5LBG5-btKqwSFqBBf4gzlohBRJgpDDYsqHYvJ4gw5VYYc13lpPWvkSqtX---xNb-IqyixMzHte9i162JmNz4nkstAMbysA0y4JymplSuxv3SDm2YpOaWUN03DG0kDBzvSk_Wydjn1iVsH9Nh6u-PedZv7rdIPsG51rVYreas2Ww0sINjjPjPB612w7jZad6qBu65dy-4RCIZeDUCvBvotQ79l2KkBowYoGDUARg3s1ZgjDJEAB8YG_XrfYXuzVssDeO_C3L_Z30tdy1XTydu61YcYxS4xD5rY113sR7tSj3_V-1-7f5f7c2To9o7e6gf180CX3Xcw22EeXBVTSKJxEn3yI5jS3-HIu7o</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>78105967</pqid></control><display><type>article</type><title>AN IMMUNOHISTOCHEMICAL STUDY OF TAL-1 PROTEIN EXPRESSION IN LEUKAEMIAS AND LYMPHOMAS WITH A NOVEL MONOCLONAL ANTIBODY, 2TL 242</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>CHETTY, RUNJAN ; PULFORD, KAREN ; JONES, MARGARET ; MATHIEU-MAHUL, DANIELE ; CLOSE, PAULINE ; HUSSEIN, SONAY ; PALLESEN, GORM ; RALFKIAER, ELIZABETH ; STEIN, HARALD ; GATTER, KEVIN ; MASON, DAVID</creator><creatorcontrib>CHETTY, RUNJAN ; PULFORD, KAREN ; JONES, MARGARET ; MATHIEU-MAHUL, DANIELE ; CLOSE, PAULINE ; HUSSEIN, SONAY ; PALLESEN, GORM ; RALFKIAER, ELIZABETH ; STEIN, HARALD ; GATTER, KEVIN ; MASON, DAVID</creatorcontrib><description>Fifty formalin fixed, paraffin‐embedded cases of T‐acute lymphoblastic leukaemia (T‐ALL) from 12 bone marrow trephines and 38 lymph nodes were stained with a new monoclonal antibody, 2TL 242, raised against recombinant TAL1 protein. The antibody recognizes TAL‐1 polypeptides of molecular weight 39 and 41 kD (full length). In addition, a variety of other leukaemias and lymphomas were also stained with 2TL 242. Twenty‐four of the 50 cases of T‐ALL showed nuclear positivity, ranging from 10 to 90 per cent of leukaemic cells. A positive staining reaction was nuclear and stippled in pattern. Nuclear staining was not seen in any other type of leukaemia or lymphoma. Five cases of follicular lymphoma showed diffuse cytoplasmic staining of variable intensity. Although some background staining is obtained with this antibody, positive nuclear staining is easily distinguishable. This monoclonal antibody has a potential role in primary diagnosis and in the detection of minimal residual disease in T‐ALL.</description><identifier>ISSN: 0022-3417</identifier><identifier>EISSN: 1096-9896</identifier><identifier>DOI: 10.1002/(SICI)1096-9896(199603)178:3<311::AID-PATH477>3.0.CO;2-B</identifier><identifier>PMID: 8778337</identifier><identifier>CODEN: JPTLAS</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Antibodies, Monoclonal - isolation & purification ; Basic Helix-Loop-Helix Transcription Factors ; Biological and medical sciences ; DNA-Binding Proteins - analysis ; Endothelium - chemistry ; Erythroid Precursor Cells - chemistry ; Hematologic and hematopoietic diseases ; Humans ; Immunohistochemistry ; Leukemia - metabolism ; Leukemia-Lymphoma, Adult T-Cell - metabolism ; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis ; Lymphoma - metabolism ; Lymphoma, Follicular - metabolism ; Medical sciences ; Megakaryocytes - chemistry ; monoclonal antibody ; Muscle, Smooth - chemistry ; Proto-Oncogene Proteins - analysis ; Sweat Glands - chemistry ; T-acute lymphoblastic leukaemia ; T-Cell Acute Lymphocytic Leukemia Protein 1 ; TAL1 gene ; Transcription Factors</subject><ispartof>The Journal of pathology, 1996-03, Vol.178 (3), p.311-315</ispartof><rights>Copyright © 1996 John Wiley & Sons, Ltd.</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c4407-f691a9a8e70dc80195a31ce4a06d48bfda93339fff9fa38202a5acdc493614c83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F%28SICI%291096-9896%28199603%29178%3A3%3C311%3A%3AAID-PATH477%3E3.0.CO%3B2-B$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F%28SICI%291096-9896%28199603%29178%3A3%3C311%3A%3AAID-PATH477%3E3.0.CO%3B2-B$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3041989$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8778337$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>CHETTY, RUNJAN</creatorcontrib><creatorcontrib>PULFORD, KAREN</creatorcontrib><creatorcontrib>JONES, MARGARET</creatorcontrib><creatorcontrib>MATHIEU-MAHUL, DANIELE</creatorcontrib><creatorcontrib>CLOSE, PAULINE</creatorcontrib><creatorcontrib>HUSSEIN, SONAY</creatorcontrib><creatorcontrib>PALLESEN, GORM</creatorcontrib><creatorcontrib>RALFKIAER, ELIZABETH</creatorcontrib><creatorcontrib>STEIN, HARALD</creatorcontrib><creatorcontrib>GATTER, KEVIN</creatorcontrib><creatorcontrib>MASON, DAVID</creatorcontrib><title>AN IMMUNOHISTOCHEMICAL STUDY OF TAL-1 PROTEIN EXPRESSION IN LEUKAEMIAS AND LYMPHOMAS WITH A NOVEL MONOCLONAL ANTIBODY, 2TL 242</title><title>The Journal of pathology</title><addtitle>J. Pathol</addtitle><description>Fifty formalin fixed, paraffin‐embedded cases of T‐acute lymphoblastic leukaemia (T‐ALL) from 12 bone marrow trephines and 38 lymph nodes were stained with a new monoclonal antibody, 2TL 242, raised against recombinant TAL1 protein. The antibody recognizes TAL‐1 polypeptides of molecular weight 39 and 41 kD (full length). In addition, a variety of other leukaemias and lymphomas were also stained with 2TL 242. Twenty‐four of the 50 cases of T‐ALL showed nuclear positivity, ranging from 10 to 90 per cent of leukaemic cells. A positive staining reaction was nuclear and stippled in pattern. Nuclear staining was not seen in any other type of leukaemia or lymphoma. Five cases of follicular lymphoma showed diffuse cytoplasmic staining of variable intensity. Although some background staining is obtained with this antibody, positive nuclear staining is easily distinguishable. This monoclonal antibody has a potential role in primary diagnosis and in the detection of minimal residual disease in T‐ALL.</description><subject>Antibodies, Monoclonal - isolation & purification</subject><subject>Basic Helix-Loop-Helix Transcription Factors</subject><subject>Biological and medical sciences</subject><subject>DNA-Binding Proteins - analysis</subject><subject>Endothelium - chemistry</subject><subject>Erythroid Precursor Cells - chemistry</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Leukemia - metabolism</subject><subject>Leukemia-Lymphoma, Adult T-Cell - metabolism</subject><subject>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</subject><subject>Lymphoma - metabolism</subject><subject>Lymphoma, Follicular - metabolism</subject><subject>Medical sciences</subject><subject>Megakaryocytes - chemistry</subject><subject>monoclonal antibody</subject><subject>Muscle, Smooth - chemistry</subject><subject>Proto-Oncogene Proteins - analysis</subject><subject>Sweat Glands - chemistry</subject><subject>T-acute lymphoblastic leukaemia</subject><subject>T-Cell Acute Lymphocytic Leukemia Protein 1</subject><subject>TAL1 gene</subject><subject>Transcription Factors</subject><issn>0022-3417</issn><issn>1096-9896</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkdFu0zAUhiMEGmXwCEi-QGiTlmLHSRx3CJGmWRNI4mpJ2SoujrzUkQLtusWrYDc8O45aegMSV1Hs_3z-dT7L-kjwkGDsvDsp0yg9JZj7Ng-4f0I49zE9JSwY0feUkNEoTCf2LKwSl7EPdIiHkTh37PETa3AYemoNDMqxqUvYc-uF1t8wxpx73pF1FDAWUMoG1q-wQGmezwuRpGUloiTO0yjMUFnNJwskLlAVZjZBs0tRxWmB4uvZZVyWqTBTBcri-efQDIQlCosJyhb5LBG5-btKqwSFqBBf4gzlohBRJgpDDYsqHYvJ4gw5VYYc13lpPWvkSqtX---xNb-IqyixMzHte9i162JmNz4nkstAMbysA0y4JymplSuxv3SDm2YpOaWUN03DG0kDBzvSk_Wydjn1iVsH9Nh6u-PedZv7rdIPsG51rVYreas2Ww0sINjjPjPB612w7jZad6qBu65dy-4RCIZeDUCvBvotQ79l2KkBowYoGDUARg3s1ZgjDJEAB8YG_XrfYXuzVssDeO_C3L_Z30tdy1XTydu61YcYxS4xD5rY113sR7tSj3_V-1-7f5f7c2To9o7e6gf180CX3Xcw22EeXBVTSKJxEn3yI5jS3-HIu7o</recordid><startdate>199603</startdate><enddate>199603</enddate><creator>CHETTY, RUNJAN</creator><creator>PULFORD, KAREN</creator><creator>JONES, MARGARET</creator><creator>MATHIEU-MAHUL, DANIELE</creator><creator>CLOSE, PAULINE</creator><creator>HUSSEIN, SONAY</creator><creator>PALLESEN, GORM</creator><creator>RALFKIAER, ELIZABETH</creator><creator>STEIN, HARALD</creator><creator>GATTER, KEVIN</creator><creator>MASON, DAVID</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199603</creationdate><title>AN IMMUNOHISTOCHEMICAL STUDY OF TAL-1 PROTEIN EXPRESSION IN LEUKAEMIAS AND LYMPHOMAS WITH A NOVEL MONOCLONAL ANTIBODY, 2TL 242</title><author>CHETTY, RUNJAN ; PULFORD, KAREN ; JONES, MARGARET ; MATHIEU-MAHUL, DANIELE ; CLOSE, PAULINE ; HUSSEIN, SONAY ; PALLESEN, GORM ; RALFKIAER, ELIZABETH ; STEIN, HARALD ; GATTER, KEVIN ; MASON, DAVID</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4407-f691a9a8e70dc80195a31ce4a06d48bfda93339fff9fa38202a5acdc493614c83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Antibodies, Monoclonal - isolation & purification</topic><topic>Basic Helix-Loop-Helix Transcription Factors</topic><topic>Biological and medical sciences</topic><topic>DNA-Binding Proteins - analysis</topic><topic>Endothelium - chemistry</topic><topic>Erythroid Precursor Cells - chemistry</topic><topic>Hematologic and hematopoietic diseases</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Leukemia - metabolism</topic><topic>Leukemia-Lymphoma, Adult T-Cell - metabolism</topic><topic>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</topic><topic>Lymphoma - metabolism</topic><topic>Lymphoma, Follicular - metabolism</topic><topic>Medical sciences</topic><topic>Megakaryocytes - chemistry</topic><topic>monoclonal antibody</topic><topic>Muscle, Smooth - chemistry</topic><topic>Proto-Oncogene Proteins - analysis</topic><topic>Sweat Glands - chemistry</topic><topic>T-acute lymphoblastic leukaemia</topic><topic>T-Cell Acute Lymphocytic Leukemia Protein 1</topic><topic>TAL1 gene</topic><topic>Transcription Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CHETTY, RUNJAN</creatorcontrib><creatorcontrib>PULFORD, KAREN</creatorcontrib><creatorcontrib>JONES, MARGARET</creatorcontrib><creatorcontrib>MATHIEU-MAHUL, DANIELE</creatorcontrib><creatorcontrib>CLOSE, PAULINE</creatorcontrib><creatorcontrib>HUSSEIN, SONAY</creatorcontrib><creatorcontrib>PALLESEN, GORM</creatorcontrib><creatorcontrib>RALFKIAER, ELIZABETH</creatorcontrib><creatorcontrib>STEIN, HARALD</creatorcontrib><creatorcontrib>GATTER, KEVIN</creatorcontrib><creatorcontrib>MASON, DAVID</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CHETTY, RUNJAN</au><au>PULFORD, KAREN</au><au>JONES, MARGARET</au><au>MATHIEU-MAHUL, DANIELE</au><au>CLOSE, PAULINE</au><au>HUSSEIN, SONAY</au><au>PALLESEN, GORM</au><au>RALFKIAER, ELIZABETH</au><au>STEIN, HARALD</au><au>GATTER, KEVIN</au><au>MASON, DAVID</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>AN IMMUNOHISTOCHEMICAL STUDY OF TAL-1 PROTEIN EXPRESSION IN LEUKAEMIAS AND LYMPHOMAS WITH A NOVEL MONOCLONAL ANTIBODY, 2TL 242</atitle><jtitle>The Journal of pathology</jtitle><addtitle>J. Pathol</addtitle><date>1996-03</date><risdate>1996</risdate><volume>178</volume><issue>3</issue><spage>311</spage><epage>315</epage><pages>311-315</pages><issn>0022-3417</issn><eissn>1096-9896</eissn><coden>JPTLAS</coden><abstract>Fifty formalin fixed, paraffin‐embedded cases of T‐acute lymphoblastic leukaemia (T‐ALL) from 12 bone marrow trephines and 38 lymph nodes were stained with a new monoclonal antibody, 2TL 242, raised against recombinant TAL1 protein. The antibody recognizes TAL‐1 polypeptides of molecular weight 39 and 41 kD (full length). In addition, a variety of other leukaemias and lymphomas were also stained with 2TL 242. Twenty‐four of the 50 cases of T‐ALL showed nuclear positivity, ranging from 10 to 90 per cent of leukaemic cells. A positive staining reaction was nuclear and stippled in pattern. Nuclear staining was not seen in any other type of leukaemia or lymphoma. Five cases of follicular lymphoma showed diffuse cytoplasmic staining of variable intensity. Although some background staining is obtained with this antibody, positive nuclear staining is easily distinguishable. This monoclonal antibody has a potential role in primary diagnosis and in the detection of minimal residual disease in T‐ALL.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>8778337</pmid><doi>10.1002/(SICI)1096-9896(199603)178:3<311::AID-PATH477>3.0.CO;2-B</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-3417 |
| ispartof | The Journal of pathology, 1996-03, Vol.178 (3), p.311-315 |
| issn | 0022-3417 1096-9896 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78105967 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Antibodies, Monoclonal - isolation & purification Basic Helix-Loop-Helix Transcription Factors Biological and medical sciences DNA-Binding Proteins - analysis Endothelium - chemistry Erythroid Precursor Cells - chemistry Hematologic and hematopoietic diseases Humans Immunohistochemistry Leukemia - metabolism Leukemia-Lymphoma, Adult T-Cell - metabolism Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis Lymphoma - metabolism Lymphoma, Follicular - metabolism Medical sciences Megakaryocytes - chemistry monoclonal antibody Muscle, Smooth - chemistry Proto-Oncogene Proteins - analysis Sweat Glands - chemistry T-acute lymphoblastic leukaemia T-Cell Acute Lymphocytic Leukemia Protein 1 TAL1 gene Transcription Factors |
| title | AN IMMUNOHISTOCHEMICAL STUDY OF TAL-1 PROTEIN EXPRESSION IN LEUKAEMIAS AND LYMPHOMAS WITH A NOVEL MONOCLONAL ANTIBODY, 2TL 242 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-21T21%3A21%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=AN%20IMMUNOHISTOCHEMICAL%20STUDY%20OF%20TAL-1%20PROTEIN%20EXPRESSION%20IN%20LEUKAEMIAS%20AND%20LYMPHOMAS%20WITH%20A%20NOVEL%20MONOCLONAL%20ANTIBODY,%202TL%20242&rft.jtitle=The%20Journal%20of%20pathology&rft.au=CHETTY,%20RUNJAN&rft.date=1996-03&rft.volume=178&rft.issue=3&rft.spage=311&rft.epage=315&rft.pages=311-315&rft.issn=0022-3417&rft.eissn=1096-9896&rft.coden=JPTLAS&rft_id=info:doi/10.1002/(SICI)1096-9896(199603)178:3%3C311::AID-PATH477%3E3.0.CO;2-B&rft_dat=%3Cproquest_cross%3E78105967%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=78105967&rft_id=info:pmid/8778337&rfr_iscdi=true |