Preparation of Liposomes That Mimic the Membrane of Endoplasmic Reticulum of Rat Hepatocytes
To examine the interaction between biomembranes and membrane-bound proteins, large unilamellar liposomes have been required. In the present study, we prepared liposomes from a mixture of phospholipids having a phospholipid composition similar to that in the endoplasmic reticula (microsomes) of rat h...
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| Veröffentlicht in: | Journal of electron microscopy 1996-04, Vol.45 (2), p.171-176 |
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| container_issue | 2 |
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| container_title | Journal of electron microscopy |
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| creator | Watanabe, Jun Asaka, Youko Mino, Kazuto Kanamura, Shinsuke |
| description | To examine the interaction between biomembranes and membrane-bound proteins, large unilamellar liposomes have been required. In the present study, we prepared liposomes from a mixture of phospholipids having a phospholipid composition similar to that in the endoplasmic reticula (microsomes) of rat hepatocytes by eight different methods. The resulting liposomes were examined by a combination of the freeze-fracture-replica procedure with biochemical methods. The freeze-thawing method of Pick (1981) gave the best results; large unilamellar liposomes that mimic the membrane of endoplasmic reticulum were obtained. Liposomes made by this method are thus suitable for analysis of the interaction between the endoplasmic reticulum membrane and membrane-bound proteins. |
| doi_str_mv | 10.1093/oxfordjournals.jmicro.a023429 |
| format | Article |
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In the present study, we prepared liposomes from a mixture of phospholipids having a phospholipid composition similar to that in the endoplasmic reticula (microsomes) of rat hepatocytes by eight different methods. The resulting liposomes were examined by a combination of the freeze-fracture-replica procedure with biochemical methods. The freeze-thawing method of Pick (1981) gave the best results; large unilamellar liposomes that mimic the membrane of endoplasmic reticulum were obtained. Liposomes made by this method are thus suitable for analysis of the interaction between the endoplasmic reticulum membrane and membrane-bound proteins.</description><identifier>ISSN: 0022-0744</identifier><identifier>EISSN: 1477-9986</identifier><identifier>DOI: 10.1093/oxfordjournals.jmicro.a023429</identifier><identifier>PMID: 8691092</identifier><language>eng</language><publisher>Japan: Oxford University Press</publisher><subject>Animals ; Cytochrome P-450 Enzyme System - metabolism ; endoplasmic reticulum ; Endoplasmic Reticulum - metabolism ; Endoplasmic Reticulum - ultrastructure ; Freeze Fracturing - methods ; freeze-fracture ; hepatocytes ; Intracellular Membranes - metabolism ; Intracellular Membranes - ultrastructure ; liposome ; Liposomes - chemistry ; Liposomes - isolation & purification ; Liver - metabolism ; Liver - ultrastructure ; Microscopy, Electron - methods ; microsome ; NADPH-Ferrihemoprotein Reductase - metabolism ; Rats ; Rats, Wistar</subject><ispartof>Journal of electron microscopy, 1996-04, Vol.45 (2), p.171-176</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c358t-60fe6f39d56c7ebd811495c0d9f73b0edac7221d5c2273c61b225f725f8818823</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8691092$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Watanabe, Jun</creatorcontrib><creatorcontrib>Asaka, Youko</creatorcontrib><creatorcontrib>Mino, Kazuto</creatorcontrib><creatorcontrib>Kanamura, Shinsuke</creatorcontrib><title>Preparation of Liposomes That Mimic the Membrane of Endoplasmic Reticulum of Rat Hepatocytes</title><title>Journal of electron microscopy</title><addtitle>J Electron Microsc (Tokyo)</addtitle><description>To examine the interaction between biomembranes and membrane-bound proteins, large unilamellar liposomes have been required. In the present study, we prepared liposomes from a mixture of phospholipids having a phospholipid composition similar to that in the endoplasmic reticula (microsomes) of rat hepatocytes by eight different methods. The resulting liposomes were examined by a combination of the freeze-fracture-replica procedure with biochemical methods. The freeze-thawing method of Pick (1981) gave the best results; large unilamellar liposomes that mimic the membrane of endoplasmic reticulum were obtained. Liposomes made by this method are thus suitable for analysis of the interaction between the endoplasmic reticulum membrane and membrane-bound proteins.</description><subject>Animals</subject><subject>Cytochrome P-450 Enzyme System - metabolism</subject><subject>endoplasmic reticulum</subject><subject>Endoplasmic Reticulum - metabolism</subject><subject>Endoplasmic Reticulum - ultrastructure</subject><subject>Freeze Fracturing - methods</subject><subject>freeze-fracture</subject><subject>hepatocytes</subject><subject>Intracellular Membranes - metabolism</subject><subject>Intracellular Membranes - ultrastructure</subject><subject>liposome</subject><subject>Liposomes - chemistry</subject><subject>Liposomes - isolation & purification</subject><subject>Liver - metabolism</subject><subject>Liver - ultrastructure</subject><subject>Microscopy, Electron - methods</subject><subject>microsome</subject><subject>NADPH-Ferrihemoprotein Reductase - metabolism</subject><subject>Rats</subject><subject>Rats, Wistar</subject><issn>0022-0744</issn><issn>1477-9986</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkF1LwzAUhoMoc05_gtAbvevMR9ukF17o3Jy6oYwJIkJI0xQ726YmKWz_3oyNgReHc_F-nMMDwBWCQwRTcqPXhTb5SnemEZUdrupSGj0UEJMIp0egjyJKwzRlyTHoQ4hxCGkUnYIza1cQIhoh2AM9lqS-DPfB15tRrTDClboJdBHMylZbXSsbLL-FC-alrw_ctwrmqs6MaNTWNG5y3VbCbrWFcqXsqq7eCgsfmfo-p-XGKXsOTgr_o7rY7wF4n4yXo2k4e318Gt3NQkli5sIEFiopSJrHiaQqyxlCURpLmKcFJRlUuZAUY5THEmNKZIIyjOOC-mEMMYbJAFzvelujfztlHa9LK1VV-X91ZzllCMYwot54uzN6ZNYaVfDWlLUwG44g39Ll_-nyHV2-p-vzl_tDXVar_JDe4_R6uNNL69T6IAvzwxNKaMynH5_8Ab6g58n9hMfkD56BjcI</recordid><startdate>19960401</startdate><enddate>19960401</enddate><creator>Watanabe, Jun</creator><creator>Asaka, Youko</creator><creator>Mino, Kazuto</creator><creator>Kanamura, Shinsuke</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19960401</creationdate><title>Preparation of Liposomes That Mimic the Membrane of Endoplasmic Reticulum of Rat Hepatocytes</title><author>Watanabe, Jun ; Asaka, Youko ; Mino, Kazuto ; Kanamura, Shinsuke</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c358t-60fe6f39d56c7ebd811495c0d9f73b0edac7221d5c2273c61b225f725f8818823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Cytochrome P-450 Enzyme System - metabolism</topic><topic>endoplasmic reticulum</topic><topic>Endoplasmic Reticulum - metabolism</topic><topic>Endoplasmic Reticulum - ultrastructure</topic><topic>Freeze Fracturing - methods</topic><topic>freeze-fracture</topic><topic>hepatocytes</topic><topic>Intracellular Membranes - metabolism</topic><topic>Intracellular Membranes - ultrastructure</topic><topic>liposome</topic><topic>Liposomes - chemistry</topic><topic>Liposomes - isolation & purification</topic><topic>Liver - metabolism</topic><topic>Liver - ultrastructure</topic><topic>Microscopy, Electron - methods</topic><topic>microsome</topic><topic>NADPH-Ferrihemoprotein Reductase - metabolism</topic><topic>Rats</topic><topic>Rats, Wistar</topic><toplevel>online_resources</toplevel><creatorcontrib>Watanabe, Jun</creatorcontrib><creatorcontrib>Asaka, Youko</creatorcontrib><creatorcontrib>Mino, Kazuto</creatorcontrib><creatorcontrib>Kanamura, Shinsuke</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of electron microscopy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Watanabe, Jun</au><au>Asaka, Youko</au><au>Mino, Kazuto</au><au>Kanamura, Shinsuke</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation of Liposomes That Mimic the Membrane of Endoplasmic Reticulum of Rat Hepatocytes</atitle><jtitle>Journal of electron microscopy</jtitle><addtitle>J Electron Microsc (Tokyo)</addtitle><date>1996-04-01</date><risdate>1996</risdate><volume>45</volume><issue>2</issue><spage>171</spage><epage>176</epage><pages>171-176</pages><issn>0022-0744</issn><eissn>1477-9986</eissn><abstract>To examine the interaction between biomembranes and membrane-bound proteins, large unilamellar liposomes have been required. 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| ispartof | Journal of electron microscopy, 1996-04, Vol.45 (2), p.171-176 |
| issn | 0022-0744 1477-9986 |
| language | eng |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Animals Cytochrome P-450 Enzyme System - metabolism endoplasmic reticulum Endoplasmic Reticulum - metabolism Endoplasmic Reticulum - ultrastructure Freeze Fracturing - methods freeze-fracture hepatocytes Intracellular Membranes - metabolism Intracellular Membranes - ultrastructure liposome Liposomes - chemistry Liposomes - isolation & purification Liver - metabolism Liver - ultrastructure Microscopy, Electron - methods microsome NADPH-Ferrihemoprotein Reductase - metabolism Rats Rats, Wistar |
| title | Preparation of Liposomes That Mimic the Membrane of Endoplasmic Reticulum of Rat Hepatocytes |
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