Enzymatic sequence-specific spin labeling of a DNA fragment containing the recognition sequence of EcoRI endonuclease

Deoxyuridine analogs spin labeled in position 5 have been enzymatically incorporated sequence specifically into an oligodeoxyribonucleotide to form a spin-labeled 26-mer. The 26-mer contains the EcoRI-binding site and two labels which are located symmetrically close to the binding site. The labels a...

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Veröffentlicht in:	FEBS letters 1988-02, Vol.228 (1), p.33-36
Hauptverfasser:	Bobst, A.M., Pauly, G.T., Keyes, R.S., Bobst, E.V.
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity Labels Analytical, structural and metabolic biochemistry Base dynamics Base Sequence Biological and medical sciences Computer Simulation Deoxyribonuclease EcoRI DNA - analysis DNA polymerase DNA Restriction Enzymes - genetics DNA-Directed DNA Polymerase Electron Spin Resonance Spectroscopy Escherichia coli - enzymology ESR Fundamental and applied biological sciences. Psychology Klenow fragment Melting profile Nucleic acids Nucleic bases, nucleotides Oligonucleotides - biosynthesis Oligonucleotides - genetics Peptide Fragments - genetics Spin-labeled oligonucleotide Temperature
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