A simple method for maintaining, detecting and recovering virulent Leishmania donovani in hamsters
The ability of hamsters ( Mesocricetus auratus) to retain amastigotes of Leishmania donovani at cutaneous sites was examined. Following intradermal inoculation of L. donovani stationary phase culture promastigotes in fore and hind footpads, nasal area and belly skin, cultures of aspirates taken fort...
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| Veröffentlicht in: | Acta tropica 1996-02, Vol.60 (4), p.263-267 |
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| creator | Anjili, C.O. Mbati, P.A. Mwangi, R.W. Githure, J.I. Koech, D.K. |
| description | The ability of hamsters (
Mesocricetus auratus) to retain amastigotes of
Leishmania donovani at cutaneous sites was examined. Following intradermal inoculation of
L. donovani stationary phase culture promastigotes in fore and hind footpads, nasal area and belly skin, cultures of aspirates taken fortnightly from these sites showed that amastigotes can survive in the skin for up to 10 months without visceralizing. Hairless cutaneous sites were better at retaining
L. donovani amastigotes than the hairy belly skin.
L. donovani promastigotes cultivated from aspirates of sites of inoculation were highly virulent. The skin is suggested as one of the sites where viscerotropic
L. donovani can remain cryptic for a long time before the infection either visceralizes or is aborted. Skins of hamsters when inoculated intradermally can serve as an easy site for maintaining, detecting and recovering virulent
L. donovani without killing the hamster. |
| doi_str_mv | 10.1016/0001-706X(95)00129-3 |
| format | Article |
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Mesocricetus auratus) to retain amastigotes of
Leishmania donovani at cutaneous sites was examined. Following intradermal inoculation of
L. donovani stationary phase culture promastigotes in fore and hind footpads, nasal area and belly skin, cultures of aspirates taken fortnightly from these sites showed that amastigotes can survive in the skin for up to 10 months without visceralizing. Hairless cutaneous sites were better at retaining
L. donovani amastigotes than the hairy belly skin.
L. donovani promastigotes cultivated from aspirates of sites of inoculation were highly virulent. The skin is suggested as one of the sites where viscerotropic
L. donovani can remain cryptic for a long time before the infection either visceralizes or is aborted. Skins of hamsters when inoculated intradermally can serve as an easy site for maintaining, detecting and recovering virulent
L. donovani without killing the hamster.</description><identifier>ISSN: 0001-706X</identifier><identifier>EISSN: 1873-6254</identifier><identifier>DOI: 10.1016/0001-706X(95)00129-3</identifier><identifier>PMID: 8659325</identifier><identifier>CODEN: ACTRAQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Cells, Cultured ; Cricetinae ; Hamsters ; Human protozoal diseases ; Infectious diseases ; Infectivity ; Injections, Intradermal ; Leishmania donovani ; Leishmania donovani - isolation & purification ; Leishmania donovani - pathogenicity ; Leshmaniasis ; Male ; Medical sciences ; Mesocricetus - parasitology ; Parasitic diseases ; Promastigotes ; Protozoal diseases ; Skin - parasitology ; Tropical medicine ; Virulence</subject><ispartof>Acta tropica, 1996-02, Vol.60 (4), p.263-267</ispartof><rights>1996</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-a98e6f9c49626b6bae9a4f4f1ec2035769814751bd6a2c78e6d053804bb1ffe33</citedby><cites>FETCH-LOGICAL-c417t-a98e6f9c49626b6bae9a4f4f1ec2035769814751bd6a2c78e6d053804bb1ffe33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0001706X95001293$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3020358$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8659325$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Anjili, C.O.</creatorcontrib><creatorcontrib>Mbati, P.A.</creatorcontrib><creatorcontrib>Mwangi, R.W.</creatorcontrib><creatorcontrib>Githure, J.I.</creatorcontrib><creatorcontrib>Koech, D.K.</creatorcontrib><title>A simple method for maintaining, detecting and recovering virulent Leishmania donovani in hamsters</title><title>Acta tropica</title><addtitle>Acta Trop</addtitle><description>The ability of hamsters (
Mesocricetus auratus) to retain amastigotes of
Leishmania donovani at cutaneous sites was examined. Following intradermal inoculation of
L. donovani stationary phase culture promastigotes in fore and hind footpads, nasal area and belly skin, cultures of aspirates taken fortnightly from these sites showed that amastigotes can survive in the skin for up to 10 months without visceralizing. Hairless cutaneous sites were better at retaining
L. donovani amastigotes than the hairy belly skin.
L. donovani promastigotes cultivated from aspirates of sites of inoculation were highly virulent. The skin is suggested as one of the sites where viscerotropic
L. donovani can remain cryptic for a long time before the infection either visceralizes or is aborted. Skins of hamsters when inoculated intradermally can serve as an easy site for maintaining, detecting and recovering virulent
L. donovani without killing the hamster.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Cricetinae</subject><subject>Hamsters</subject><subject>Human protozoal diseases</subject><subject>Infectious diseases</subject><subject>Infectivity</subject><subject>Injections, Intradermal</subject><subject>Leishmania donovani</subject><subject>Leishmania donovani - isolation & purification</subject><subject>Leishmania donovani - pathogenicity</subject><subject>Leshmaniasis</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mesocricetus - parasitology</subject><subject>Parasitic diseases</subject><subject>Promastigotes</subject><subject>Protozoal diseases</subject><subject>Skin - parasitology</subject><subject>Tropical medicine</subject><subject>Virulence</subject><issn>0001-706X</issn><issn>1873-6254</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkd-rFCEUxyWK23brPyjwIaKgKR1HHV8uXC79goVeCnoTR4-tMaObugv99zl3l32sB_Er53MOx-8XoeeUvKOEiveEENpJIn68VvxN073q2AO0oaNknej58BBtLshj9KSUXyskeX-FrkbBFev5Bk23uIRlPwNeoO6Swz5lvJgQazsh_nyLHVSwtUlsosMZbDpCXp_HkA8zxIq3EMpuMTEY7FJMx6ZwiHhnllIhl6fokTdzgWfn-xp9__jh293nbvv105e7221nByprZ9QIwis7KNGLSUwGlBn84CnYnjAuhRrpIDmdnDC9lQ12hLORDNNEvQfGrtGr09x9Tr8PUKpeQrEwzyZCOhQtx2abbD3_A1kvBOf34HACbU6lZPB6n8Ni8h9NiV4z0KvBejVYK67vM9DrIi_O8w_TAu7SdDa91V-e66ZYM_tsog3lgjGy_nds2M0Jg2baMUDWxQaIFlxoKVTtUvj3Hn8B9zGjWg</recordid><startdate>19960201</startdate><enddate>19960201</enddate><creator>Anjili, C.O.</creator><creator>Mbati, P.A.</creator><creator>Mwangi, R.W.</creator><creator>Githure, J.I.</creator><creator>Koech, D.K.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>19960201</creationdate><title>A simple method for maintaining, detecting and recovering virulent Leishmania donovani in hamsters</title><author>Anjili, C.O. ; Mbati, P.A. ; Mwangi, R.W. ; Githure, J.I. ; Koech, D.K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-a98e6f9c49626b6bae9a4f4f1ec2035769814751bd6a2c78e6d053804bb1ffe33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Cricetinae</topic><topic>Hamsters</topic><topic>Human protozoal diseases</topic><topic>Infectious diseases</topic><topic>Infectivity</topic><topic>Injections, Intradermal</topic><topic>Leishmania donovani</topic><topic>Leishmania donovani - isolation & purification</topic><topic>Leishmania donovani - pathogenicity</topic><topic>Leshmaniasis</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mesocricetus - parasitology</topic><topic>Parasitic diseases</topic><topic>Promastigotes</topic><topic>Protozoal diseases</topic><topic>Skin - parasitology</topic><topic>Tropical medicine</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Anjili, C.O.</creatorcontrib><creatorcontrib>Mbati, P.A.</creatorcontrib><creatorcontrib>Mwangi, R.W.</creatorcontrib><creatorcontrib>Githure, J.I.</creatorcontrib><creatorcontrib>Koech, D.K.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Acta tropica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Anjili, C.O.</au><au>Mbati, P.A.</au><au>Mwangi, R.W.</au><au>Githure, J.I.</au><au>Koech, D.K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A simple method for maintaining, detecting and recovering virulent Leishmania donovani in hamsters</atitle><jtitle>Acta tropica</jtitle><addtitle>Acta Trop</addtitle><date>1996-02-01</date><risdate>1996</risdate><volume>60</volume><issue>4</issue><spage>263</spage><epage>267</epage><pages>263-267</pages><issn>0001-706X</issn><eissn>1873-6254</eissn><coden>ACTRAQ</coden><abstract>The ability of hamsters (
Mesocricetus auratus) to retain amastigotes of
Leishmania donovani at cutaneous sites was examined. Following intradermal inoculation of
L. donovani stationary phase culture promastigotes in fore and hind footpads, nasal area and belly skin, cultures of aspirates taken fortnightly from these sites showed that amastigotes can survive in the skin for up to 10 months without visceralizing. Hairless cutaneous sites were better at retaining
L. donovani amastigotes than the hairy belly skin.
L. donovani promastigotes cultivated from aspirates of sites of inoculation were highly virulent. The skin is suggested as one of the sites where viscerotropic
L. donovani can remain cryptic for a long time before the infection either visceralizes or is aborted. Skins of hamsters when inoculated intradermally can serve as an easy site for maintaining, detecting and recovering virulent
L. donovani without killing the hamster.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>8659325</pmid><doi>10.1016/0001-706X(95)00129-3</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Acta tropica, 1996-02, Vol.60 (4), p.263-267 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Biological and medical sciences Cells, Cultured Cricetinae Hamsters Human protozoal diseases Infectious diseases Infectivity Injections, Intradermal Leishmania donovani Leishmania donovani - isolation & purification Leishmania donovani - pathogenicity Leshmaniasis Male Medical sciences Mesocricetus - parasitology Parasitic diseases Promastigotes Protozoal diseases Skin - parasitology Tropical medicine Virulence |
| title | A simple method for maintaining, detecting and recovering virulent Leishmania donovani in hamsters |
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