Equine arteritis virus subgenomic mRNA synthesis: analysis of leader-body junctions and replicative-form RNAs
In addition to the genomic RNA, a 3' coterminal nested set of six subgenomic mRNAs is produced in equine arteritis virus (EAV)-infected cells. The seven viral RNAs are also 5' coterminal, since they all contain a 206-nucleotide common leader sequence which is identical to the 5' end o...
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| Veröffentlicht in: | Journal of Virology 1996-07, Vol.70 (7), p.4291-4298 |
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| container_title | Journal of Virology |
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| creator | Boon, J.A. den (Leiden University, Leiden, The Netherlands.) Kleijnen, M.F Spaan, W.J.M Snijder, E.J |
| description | In addition to the genomic RNA, a 3' coterminal nested set of six subgenomic mRNAs is produced in equine arteritis virus (EAV)-infected cells. The seven viral RNAs are also 5' coterminal, since they all contain a 206-nucleotide common leader sequence which is identical to the 5' end of the genome. A conserved pentanucleotide sequence motif, 5' UCAAC 3', was shown to be present at the junctions between the leader and body sequences in each of the mRNAs. In addition, two alternative junction sites were detected for mRNA 3. Seven replicative-form (RF) RNAs (RFs I to VII), corresponding to the genomic RNA and each of the subgenomic EAV mRNAs, could be prepared from lysates of infected cells. The minus-strand RNA contents of these RF RNAs were analyzed by using an RNase protection assay with an RNA probe containing the mRNA 2 leader-body junction. It was established that RF 11 contained a negative-stranded copy of mRNA 2, including a complementary leader sequence. The presence of subgenomic minus-strand RNA in RFs is indicative of a function as a transcription template during the production of EAV subgenomic mRNAs |
| doi_str_mv | 10.1128/jvi.70.7.4291-4298.1996 |
| format | Article |
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The seven viral RNAs are also 5' coterminal, since they all contain a 206-nucleotide common leader sequence which is identical to the 5' end of the genome. A conserved pentanucleotide sequence motif, 5' UCAAC 3', was shown to be present at the junctions between the leader and body sequences in each of the mRNAs. In addition, two alternative junction sites were detected for mRNA 3. Seven replicative-form (RF) RNAs (RFs I to VII), corresponding to the genomic RNA and each of the subgenomic EAV mRNAs, could be prepared from lysates of infected cells. The minus-strand RNA contents of these RF RNAs were analyzed by using an RNase protection assay with an RNA probe containing the mRNA 2 leader-body junction. It was established that RF 11 contained a negative-stranded copy of mRNA 2, including a complementary leader sequence. 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The seven viral RNAs are also 5' coterminal, since they all contain a 206-nucleotide common leader sequence which is identical to the 5' end of the genome. A conserved pentanucleotide sequence motif, 5' UCAAC 3', was shown to be present at the junctions between the leader and body sequences in each of the mRNAs. In addition, two alternative junction sites were detected for mRNA 3. Seven replicative-form (RF) RNAs (RFs I to VII), corresponding to the genomic RNA and each of the subgenomic EAV mRNAs, could be prepared from lysates of infected cells. The minus-strand RNA contents of these RF RNAs were analyzed by using an RNase protection assay with an RNA probe containing the mRNA 2 leader-body junction. It was established that RF 11 contained a negative-stranded copy of mRNA 2, including a complementary leader sequence. 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Kleijnen, M.F ; Spaan, W.J.M ; Snijder, E.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c607t-8bb2ce5b7cbce53dfb7c3b25d883137912ed2db54853629e88f381d5de1a1e473</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>ADN</topic><topic>Animals</topic><topic>ARN</topic><topic>ARN MENSAJERO</topic><topic>ARN MESSAGER</topic><topic>Arteritis Virus, Equine - genetics</topic><topic>Base Sequence</topic><topic>Cell Line</topic><topic>Conserved Sequence</topic><topic>Cricetinae</topic><topic>equine arteritis virus</topic><topic>Genome, Viral</topic><topic>Molecular Sequence Data</topic><topic>Oligonucleotide Probes</topic><topic>PESTIVIRUS</topic><topic>RNA, Messenger - biosynthesis</topic><topic>RNA, Viral - biosynthesis</topic><topic>SECUENCIA NUCLEOTIDICA</topic><topic>SEQUENCE NUCLEOTIDIQUE</topic><topic>VARIACION GENETICA</topic><topic>VARIATION GENETIQUE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Boon, J.A. den (Leiden University, Leiden, The Netherlands.)</creatorcontrib><creatorcontrib>Kleijnen, M.F</creatorcontrib><creatorcontrib>Spaan, W.J.M</creatorcontrib><creatorcontrib>Snijder, E.J</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boon, J.A. den (Leiden University, Leiden, The Netherlands.)</au><au>Kleijnen, M.F</au><au>Spaan, W.J.M</au><au>Snijder, E.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Equine arteritis virus subgenomic mRNA synthesis: analysis of leader-body junctions and replicative-form RNAs</atitle><jtitle>Journal of Virology</jtitle><addtitle>J Virol</addtitle><date>1996-07-01</date><risdate>1996</risdate><volume>70</volume><issue>7</issue><spage>4291</spage><epage>4298</epage><pages>4291-4298</pages><issn>0022-538X</issn><eissn>1098-5514</eissn><abstract>In addition to the genomic RNA, a 3' coterminal nested set of six subgenomic mRNAs is produced in equine arteritis virus (EAV)-infected cells. 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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | ADN Animals ARN ARN MENSAJERO ARN MESSAGER Arteritis Virus, Equine - genetics Base Sequence Cell Line Conserved Sequence Cricetinae equine arteritis virus Genome, Viral Molecular Sequence Data Oligonucleotide Probes PESTIVIRUS RNA, Messenger - biosynthesis RNA, Viral - biosynthesis SECUENCIA NUCLEOTIDICA SEQUENCE NUCLEOTIDIQUE VARIACION GENETICA VARIATION GENETIQUE |
| title | Equine arteritis virus subgenomic mRNA synthesis: analysis of leader-body junctions and replicative-form RNAs |
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