Modulation of Proliferation, Second Messenger Levels, and Morphotype Expression of the Rat Intestinal Epithelial Cell Line IEC-6 by Fermented Milk
Trophic effects of milk fermented with Lactobacillus helveticus, Lactobacillus paracasei ssp. paracasei, Bifidobacterium sp., or the combination of Lactobacillus bulgaricus and Streptococcus thermophilus (yogurt) were studied on the IEC-6 intestinal epithelial cell line. Incorporation of methyl-3Hth...
Gespeichert in:
| Veröffentlicht in: | Journal of dairy science 1996-01, Vol.79 (1), p.33-43 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 43 |
|---|---|
| container_issue | 1 |
| container_start_page | 33 |
| container_title | Journal of dairy science |
| container_volume | 79 |
| creator | Thoreux, Karine Senegas-Balas, Francoise Bernard-Perrone, Francoise Giannarelli, Sylvie Denariaz, Gerard Bouley, Christine Balas, Daniel |
| description | Trophic effects of milk fermented with Lactobacillus helveticus, Lactobacillus paracasei ssp. paracasei, Bifidobacterium sp., or the combination of Lactobacillus bulgaricus and Streptococcus thermophilus (yogurt) were studied on the IEC-6 intestinal epithelial cell line. Incorporation of methyl-3Hthymidine, mitochondrial dehydrogenase activities, cyclic AMP production, ad differentiation of levels of the IEC-6 strain were evaluated between the 15th and 30th passage in culture. All fermented and unfermented milks enhanced trophic responses of IEC-6 cells in a dose-dependent manner. Compared with the corresponding milks, supernatant fractions were more effective in stimulating mitochondrial dehydrogenase response. Fermented milk supernatants were also more effective than the corresponding unfermented fractions. Increases in DNA synthesis and cyclic AMP confirmed the activation observed with mitochondrial dehydrogenase. Yogurt induced the more trophic response with an increased number of the more differentiated cell morphotype. Fermentation with L. casei also demonstrated an important trophic adaptation of IEC-6 cells. Milk processing by lactic acid bacteria enhanced trophic and proliferation responses of intestinal epithelial cell line IEC-6. These results suggested that IEC-6 cells could represent an accurate and easy in vitro model for testing the trophic quality of various nutrients and for an optimization of physioloical digestive functions. |
| doi_str_mv | 10.3168/jds.S0022-0302(96)76331-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_78093253</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0022030296763312</els_id><sourcerecordid>1877095377</sourcerecordid><originalsourceid>FETCH-LOGICAL-c499t-b4406dadfce5efa8a43dc7336050eb4c069c6245328a311de1e75493c069872e3</originalsourceid><addsrcrecordid>eNqNkc1uEzEUhUcIVErhBZCQjEAIpE7xz9geL1GUQqRUIELXluO5kzg446k9KeQ1eGI8SdQFK1a27_nu8bVPUbwm-IoRUX_cNOlqgTGlJWaYvlfigxSMkZI-Ks4Jp7xkRNWPi_MH5GnxLKVNPhKK-VlxVgvJZY3Piz83odl5M7jQodCibzF410I8FC7RAmzoGnQDKUG3gojmcA8-XSIzVkPs12HY94Cmv_uYmZPJsAb03Qxo1g2QBtcZj6a9y1Xv8nYC3qO56wDNppNSoOUeXUPcQoazp_M_nxdPWuMTvDitF8Xt9fTH5Es5__p5Nvk0L22l1FAuqwqLxjStBQ6tqU3FGisZE5hjWFYWC2UFrTijtWGENEBA8kqxUaglBXZRvDv69jHc7fKkeuuSzdOZDsIu6fw_ilHOMvjmH3ATdjE_K2lSS4kVZ1JmSh0pG0NKEVrdR7c1ca8J1mNqOqemD6npMRKthD6kpmnufXW6YbfcQvPQeYop62-P-tqt1r9cBJ22xvtMk9FVKk00Gwd9ecRaE7RZRZf07UIJwitWZ3FyFHOAcO8g6mQddBaa7GcH3QT3H5P-BYKvvuE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1877095377</pqid></control><display><type>article</type><title>Modulation of Proliferation, Second Messenger Levels, and Morphotype Expression of the Rat Intestinal Epithelial Cell Line IEC-6 by Fermented Milk</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Periodicals Index Online</source><creator>Thoreux, Karine ; Senegas-Balas, Francoise ; Bernard-Perrone, Francoise ; Giannarelli, Sylvie ; Denariaz, Gerard ; Bouley, Christine ; Balas, Daniel</creator><creatorcontrib>Thoreux, Karine ; Senegas-Balas, Francoise ; Bernard-Perrone, Francoise ; Giannarelli, Sylvie ; Denariaz, Gerard ; Bouley, Christine ; Balas, Daniel</creatorcontrib><description>Trophic effects of milk fermented with Lactobacillus helveticus, Lactobacillus paracasei ssp. paracasei, Bifidobacterium sp., or the combination of Lactobacillus bulgaricus and Streptococcus thermophilus (yogurt) were studied on the IEC-6 intestinal epithelial cell line. Incorporation of methyl-3Hthymidine, mitochondrial dehydrogenase activities, cyclic AMP production, ad differentiation of levels of the IEC-6 strain were evaluated between the 15th and 30th passage in culture. All fermented and unfermented milks enhanced trophic responses of IEC-6 cells in a dose-dependent manner. Compared with the corresponding milks, supernatant fractions were more effective in stimulating mitochondrial dehydrogenase response. Fermented milk supernatants were also more effective than the corresponding unfermented fractions. Increases in DNA synthesis and cyclic AMP confirmed the activation observed with mitochondrial dehydrogenase. Yogurt induced the more trophic response with an increased number of the more differentiated cell morphotype. Fermentation with L. casei also demonstrated an important trophic adaptation of IEC-6 cells. Milk processing by lactic acid bacteria enhanced trophic and proliferation responses of intestinal epithelial cell line IEC-6. These results suggested that IEC-6 cells could represent an accurate and easy in vitro model for testing the trophic quality of various nutrients and for an optimization of physioloical digestive functions.</description><identifier>ISSN: 0022-0302</identifier><identifier>EISSN: 1525-3198</identifier><identifier>DOI: 10.3168/jds.S0022-0302(96)76331-2</identifier><identifier>PMID: 8675780</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>ADENOSINE MONOPHOSPHATE ; ADENOSINMONOFOSFATO ; ADN ; ANIMAL NOUVEAU NE ; ANIMAL RECIEN NACIDO ; Animals ; BIFIDOBACTERIUM ; Bifidobacterium - metabolism ; Cell Division ; Cell Line ; cell proliferation ; CULTIVO DE CELULAS ; CULTURE DE CELLULE ; Cyclic AMP - metabolism ; DNA - biosynthesis ; Epithelial Cells ; Epithelium - metabolism ; EXPERIMENTACION ; EXPERIMENTATION ; Fermentation ; fermented milk ; IEC-6 cells ; INTESTIN ; Intestines - cytology ; Intestines - metabolism ; INTESTINOS ; lactic acid bacteria ; LACTOBACILLUS ; Lactobacillus - metabolism ; LACTOBACILLUS BULGARICUS ; LACTOBACILLUS HELVETICUS ; LAIT FERMENTE ; LECHE FERMENTADA ; LECTINAS ; LECTINE ; MEMBRANA MUCOSA ; Milk - microbiology ; Milk - physiology ; MITOCHONDRIE ; MITOCONDRIA ; MUQUEUSE ; Rats ; Second Messenger Systems ; STREPTOCOCCUS THERMOPHILUS ; Thymidine - analogs & derivatives ; Thymidine - metabolism ; YAOURT ; YOGUR ; Yogurt</subject><ispartof>Journal of dairy science, 1996-01, Vol.79 (1), p.33-43</ispartof><rights>1996 American Dairy Science Association</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-b4406dadfce5efa8a43dc7336050eb4c069c6245328a311de1e75493c069872e3</citedby><cites>FETCH-LOGICAL-c499t-b4406dadfce5efa8a43dc7336050eb4c069c6245328a311de1e75493c069872e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.3168/jds.S0022-0302(96)76331-2$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27869,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8675780$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thoreux, Karine</creatorcontrib><creatorcontrib>Senegas-Balas, Francoise</creatorcontrib><creatorcontrib>Bernard-Perrone, Francoise</creatorcontrib><creatorcontrib>Giannarelli, Sylvie</creatorcontrib><creatorcontrib>Denariaz, Gerard</creatorcontrib><creatorcontrib>Bouley, Christine</creatorcontrib><creatorcontrib>Balas, Daniel</creatorcontrib><title>Modulation of Proliferation, Second Messenger Levels, and Morphotype Expression of the Rat Intestinal Epithelial Cell Line IEC-6 by Fermented Milk</title><title>Journal of dairy science</title><addtitle>J Dairy Sci</addtitle><description>Trophic effects of milk fermented with Lactobacillus helveticus, Lactobacillus paracasei ssp. paracasei, Bifidobacterium sp., or the combination of Lactobacillus bulgaricus and Streptococcus thermophilus (yogurt) were studied on the IEC-6 intestinal epithelial cell line. Incorporation of methyl-3Hthymidine, mitochondrial dehydrogenase activities, cyclic AMP production, ad differentiation of levels of the IEC-6 strain were evaluated between the 15th and 30th passage in culture. All fermented and unfermented milks enhanced trophic responses of IEC-6 cells in a dose-dependent manner. Compared with the corresponding milks, supernatant fractions were more effective in stimulating mitochondrial dehydrogenase response. Fermented milk supernatants were also more effective than the corresponding unfermented fractions. Increases in DNA synthesis and cyclic AMP confirmed the activation observed with mitochondrial dehydrogenase. Yogurt induced the more trophic response with an increased number of the more differentiated cell morphotype. Fermentation with L. casei also demonstrated an important trophic adaptation of IEC-6 cells. Milk processing by lactic acid bacteria enhanced trophic and proliferation responses of intestinal epithelial cell line IEC-6. These results suggested that IEC-6 cells could represent an accurate and easy in vitro model for testing the trophic quality of various nutrients and for an optimization of physioloical digestive functions.</description><subject>ADENOSINE MONOPHOSPHATE</subject><subject>ADENOSINMONOFOSFATO</subject><subject>ADN</subject><subject>ANIMAL NOUVEAU NE</subject><subject>ANIMAL RECIEN NACIDO</subject><subject>Animals</subject><subject>BIFIDOBACTERIUM</subject><subject>Bifidobacterium - metabolism</subject><subject>Cell Division</subject><subject>Cell Line</subject><subject>cell proliferation</subject><subject>CULTIVO DE CELULAS</subject><subject>CULTURE DE CELLULE</subject><subject>Cyclic AMP - metabolism</subject><subject>DNA - biosynthesis</subject><subject>Epithelial Cells</subject><subject>Epithelium - metabolism</subject><subject>EXPERIMENTACION</subject><subject>EXPERIMENTATION</subject><subject>Fermentation</subject><subject>fermented milk</subject><subject>IEC-6 cells</subject><subject>INTESTIN</subject><subject>Intestines - cytology</subject><subject>Intestines - metabolism</subject><subject>INTESTINOS</subject><subject>lactic acid bacteria</subject><subject>LACTOBACILLUS</subject><subject>Lactobacillus - metabolism</subject><subject>LACTOBACILLUS BULGARICUS</subject><subject>LACTOBACILLUS HELVETICUS</subject><subject>LAIT FERMENTE</subject><subject>LECHE FERMENTADA</subject><subject>LECTINAS</subject><subject>LECTINE</subject><subject>MEMBRANA MUCOSA</subject><subject>Milk - microbiology</subject><subject>Milk - physiology</subject><subject>MITOCHONDRIE</subject><subject>MITOCONDRIA</subject><subject>MUQUEUSE</subject><subject>Rats</subject><subject>Second Messenger Systems</subject><subject>STREPTOCOCCUS THERMOPHILUS</subject><subject>Thymidine - analogs & derivatives</subject><subject>Thymidine - metabolism</subject><subject>YAOURT</subject><subject>YOGUR</subject><subject>Yogurt</subject><issn>0022-0302</issn><issn>1525-3198</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>K30</sourceid><recordid>eNqNkc1uEzEUhUcIVErhBZCQjEAIpE7xz9geL1GUQqRUIELXluO5kzg446k9KeQ1eGI8SdQFK1a27_nu8bVPUbwm-IoRUX_cNOlqgTGlJWaYvlfigxSMkZI-Ks4Jp7xkRNWPi_MH5GnxLKVNPhKK-VlxVgvJZY3Piz83odl5M7jQodCibzF410I8FC7RAmzoGnQDKUG3gojmcA8-XSIzVkPs12HY94Cmv_uYmZPJsAb03Qxo1g2QBtcZj6a9y1Xv8nYC3qO56wDNppNSoOUeXUPcQoazp_M_nxdPWuMTvDitF8Xt9fTH5Es5__p5Nvk0L22l1FAuqwqLxjStBQ6tqU3FGisZE5hjWFYWC2UFrTijtWGENEBA8kqxUaglBXZRvDv69jHc7fKkeuuSzdOZDsIu6fw_ilHOMvjmH3ATdjE_K2lSS4kVZ1JmSh0pG0NKEVrdR7c1ca8J1mNqOqemD6npMRKthD6kpmnufXW6YbfcQvPQeYop62-P-tqt1r9cBJ22xvtMk9FVKk00Gwd9ecRaE7RZRZf07UIJwitWZ3FyFHOAcO8g6mQddBaa7GcH3QT3H5P-BYKvvuE</recordid><startdate>199601</startdate><enddate>199601</enddate><creator>Thoreux, Karine</creator><creator>Senegas-Balas, Francoise</creator><creator>Bernard-Perrone, Francoise</creator><creator>Giannarelli, Sylvie</creator><creator>Denariaz, Gerard</creator><creator>Bouley, Christine</creator><creator>Balas, Daniel</creator><general>Elsevier Inc</general><general>Am Dairy Sci Assoc</general><general>American Dairy Science Association</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7WH</scope><scope>K30</scope><scope>PAAUG</scope><scope>PAWHS</scope><scope>PAWZZ</scope><scope>PAXOH</scope><scope>PBHAV</scope><scope>PBQSW</scope><scope>PBYQZ</scope><scope>PCIWU</scope><scope>PCMID</scope><scope>PCZJX</scope><scope>PDGRG</scope><scope>PDWWI</scope><scope>PETMR</scope><scope>PFVGT</scope><scope>PGXDX</scope><scope>PIHIL</scope><scope>PISVA</scope><scope>PJCTQ</scope><scope>PJTMS</scope><scope>PLCHJ</scope><scope>PMHAD</scope><scope>PNQDJ</scope><scope>POUND</scope><scope>PPLAD</scope><scope>PQAPC</scope><scope>PQCAN</scope><scope>PQCMW</scope><scope>PQEME</scope><scope>PQHKH</scope><scope>PQMID</scope><scope>PQNCT</scope><scope>PQNET</scope><scope>PQSCT</scope><scope>PQSET</scope><scope>PSVJG</scope><scope>PVMQY</scope><scope>PZGFC</scope><scope>7X8</scope></search><sort><creationdate>199601</creationdate><title>Modulation of Proliferation, Second Messenger Levels, and Morphotype Expression of the Rat Intestinal Epithelial Cell Line IEC-6 by Fermented Milk</title><author>Thoreux, Karine ; Senegas-Balas, Francoise ; Bernard-Perrone, Francoise ; Giannarelli, Sylvie ; Denariaz, Gerard ; Bouley, Christine ; Balas, Daniel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-b4406dadfce5efa8a43dc7336050eb4c069c6245328a311de1e75493c069872e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>ADENOSINE MONOPHOSPHATE</topic><topic>ADENOSINMONOFOSFATO</topic><topic>ADN</topic><topic>ANIMAL NOUVEAU NE</topic><topic>ANIMAL RECIEN NACIDO</topic><topic>Animals</topic><topic>BIFIDOBACTERIUM</topic><topic>Bifidobacterium - metabolism</topic><topic>Cell Division</topic><topic>Cell Line</topic><topic>cell proliferation</topic><topic>CULTIVO DE CELULAS</topic><topic>CULTURE DE CELLULE</topic><topic>Cyclic AMP - metabolism</topic><topic>DNA - biosynthesis</topic><topic>Epithelial Cells</topic><topic>Epithelium - metabolism</topic><topic>EXPERIMENTACION</topic><topic>EXPERIMENTATION</topic><topic>Fermentation</topic><topic>fermented milk</topic><topic>IEC-6 cells</topic><topic>INTESTIN</topic><topic>Intestines - cytology</topic><topic>Intestines - metabolism</topic><topic>INTESTINOS</topic><topic>lactic acid bacteria</topic><topic>LACTOBACILLUS</topic><topic>Lactobacillus - metabolism</topic><topic>LACTOBACILLUS BULGARICUS</topic><topic>LACTOBACILLUS HELVETICUS</topic><topic>LAIT FERMENTE</topic><topic>LECHE FERMENTADA</topic><topic>LECTINAS</topic><topic>LECTINE</topic><topic>MEMBRANA MUCOSA</topic><topic>Milk - microbiology</topic><topic>Milk - physiology</topic><topic>MITOCHONDRIE</topic><topic>MITOCONDRIA</topic><topic>MUQUEUSE</topic><topic>Rats</topic><topic>Second Messenger Systems</topic><topic>STREPTOCOCCUS THERMOPHILUS</topic><topic>Thymidine - analogs & derivatives</topic><topic>Thymidine - metabolism</topic><topic>YAOURT</topic><topic>YOGUR</topic><topic>Yogurt</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thoreux, Karine</creatorcontrib><creatorcontrib>Senegas-Balas, Francoise</creatorcontrib><creatorcontrib>Bernard-Perrone, Francoise</creatorcontrib><creatorcontrib>Giannarelli, Sylvie</creatorcontrib><creatorcontrib>Denariaz, Gerard</creatorcontrib><creatorcontrib>Bouley, Christine</creatorcontrib><creatorcontrib>Balas, Daniel</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Periodicals Index Online Segment 50</collection><collection>Periodicals Index Online</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - West</collection><collection>Primary Sources Access (Plan D) - International</collection><collection>Primary Sources Access & Build (Plan A) - MEA</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - Midwest</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - Northeast</collection><collection>Primary Sources Access (Plan D) - Southeast</collection><collection>Primary Sources Access (Plan D) - North Central</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - Southeast</collection><collection>Primary Sources Access (Plan D) - South Central</collection><collection>Primary Sources Access & Build (Plan A) - UK / I</collection><collection>Primary Sources Access (Plan D) - Canada</collection><collection>Primary Sources Access (Plan D) - EMEALA</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - North Central</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - South Central</collection><collection>Primary Sources Access & Build (Plan A) - International</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - International</collection><collection>Primary Sources Access (Plan D) - West</collection><collection>Periodicals Index Online Segments 1-50</collection><collection>Primary Sources Access (Plan D) - APAC</collection><collection>Primary Sources Access (Plan D) - Midwest</collection><collection>Primary Sources Access (Plan D) - MEA</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - Canada</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - UK / I</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - EMEALA</collection><collection>Primary Sources Access & Build (Plan A) - APAC</collection><collection>Primary Sources Access & Build (Plan A) - Canada</collection><collection>Primary Sources Access & Build (Plan A) - West</collection><collection>Primary Sources Access & Build (Plan A) - EMEALA</collection><collection>Primary Sources Access (Plan D) - Northeast</collection><collection>Primary Sources Access & Build (Plan A) - Midwest</collection><collection>Primary Sources Access & Build (Plan A) - North Central</collection><collection>Primary Sources Access & Build (Plan A) - Northeast</collection><collection>Primary Sources Access & Build (Plan A) - South Central</collection><collection>Primary Sources Access & Build (Plan A) - Southeast</collection><collection>Primary Sources Access (Plan D) - UK / I</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - APAC</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - MEA</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of dairy science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thoreux, Karine</au><au>Senegas-Balas, Francoise</au><au>Bernard-Perrone, Francoise</au><au>Giannarelli, Sylvie</au><au>Denariaz, Gerard</au><au>Bouley, Christine</au><au>Balas, Daniel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modulation of Proliferation, Second Messenger Levels, and Morphotype Expression of the Rat Intestinal Epithelial Cell Line IEC-6 by Fermented Milk</atitle><jtitle>Journal of dairy science</jtitle><addtitle>J Dairy Sci</addtitle><date>1996-01</date><risdate>1996</risdate><volume>79</volume><issue>1</issue><spage>33</spage><epage>43</epage><pages>33-43</pages><issn>0022-0302</issn><eissn>1525-3198</eissn><abstract>Trophic effects of milk fermented with Lactobacillus helveticus, Lactobacillus paracasei ssp. paracasei, Bifidobacterium sp., or the combination of Lactobacillus bulgaricus and Streptococcus thermophilus (yogurt) were studied on the IEC-6 intestinal epithelial cell line. Incorporation of methyl-3Hthymidine, mitochondrial dehydrogenase activities, cyclic AMP production, ad differentiation of levels of the IEC-6 strain were evaluated between the 15th and 30th passage in culture. All fermented and unfermented milks enhanced trophic responses of IEC-6 cells in a dose-dependent manner. Compared with the corresponding milks, supernatant fractions were more effective in stimulating mitochondrial dehydrogenase response. Fermented milk supernatants were also more effective than the corresponding unfermented fractions. Increases in DNA synthesis and cyclic AMP confirmed the activation observed with mitochondrial dehydrogenase. Yogurt induced the more trophic response with an increased number of the more differentiated cell morphotype. Fermentation with L. casei also demonstrated an important trophic adaptation of IEC-6 cells. Milk processing by lactic acid bacteria enhanced trophic and proliferation responses of intestinal epithelial cell line IEC-6. These results suggested that IEC-6 cells could represent an accurate and easy in vitro model for testing the trophic quality of various nutrients and for an optimization of physioloical digestive functions.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>8675780</pmid><doi>10.3168/jds.S0022-0302(96)76331-2</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-0302 |
| ispartof | Journal of dairy science, 1996-01, Vol.79 (1), p.33-43 |
| issn | 0022-0302 1525-3198 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78093253 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Periodicals Index Online |
| subjects | ADENOSINE MONOPHOSPHATE ADENOSINMONOFOSFATO ADN ANIMAL NOUVEAU NE ANIMAL RECIEN NACIDO Animals BIFIDOBACTERIUM Bifidobacterium - metabolism Cell Division Cell Line cell proliferation CULTIVO DE CELULAS CULTURE DE CELLULE Cyclic AMP - metabolism DNA - biosynthesis Epithelial Cells Epithelium - metabolism EXPERIMENTACION EXPERIMENTATION Fermentation fermented milk IEC-6 cells INTESTIN Intestines - cytology Intestines - metabolism INTESTINOS lactic acid bacteria LACTOBACILLUS Lactobacillus - metabolism LACTOBACILLUS BULGARICUS LACTOBACILLUS HELVETICUS LAIT FERMENTE LECHE FERMENTADA LECTINAS LECTINE MEMBRANA MUCOSA Milk - microbiology Milk - physiology MITOCHONDRIE MITOCONDRIA MUQUEUSE Rats Second Messenger Systems STREPTOCOCCUS THERMOPHILUS Thymidine - analogs & derivatives Thymidine - metabolism YAOURT YOGUR Yogurt |
| title | Modulation of Proliferation, Second Messenger Levels, and Morphotype Expression of the Rat Intestinal Epithelial Cell Line IEC-6 by Fermented Milk |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T11%3A52%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Modulation%20of%20Proliferation,%20Second%20Messenger%20Levels,%20and%20Morphotype%20Expression%20of%20the%20Rat%20Intestinal%20Epithelial%20Cell%20Line%20IEC-6%20by%20Fermented%20Milk&rft.jtitle=Journal%20of%20dairy%20science&rft.au=Thoreux,%20Karine&rft.date=1996-01&rft.volume=79&rft.issue=1&rft.spage=33&rft.epage=43&rft.pages=33-43&rft.issn=0022-0302&rft.eissn=1525-3198&rft_id=info:doi/10.3168/jds.S0022-0302(96)76331-2&rft_dat=%3Cproquest_cross%3E1877095377%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1877095377&rft_id=info:pmid/8675780&rft_els_id=S0022030296763312&rfr_iscdi=true |